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In order to strengthen the rapid and stable formation of hydrostatically oxygenic photogranules (HSP), a strategy of inoculating Oscillatoria sp., a typical kind of filamentous cyanobacteria, into activated sludge at the early stage of cultivation was proposed. The photogranulation process, morphology, extracellular polymeric substances contents, surface thermodynamics properties and microbial community were analyzed to elucidate the mechanism for efficient photogranulation in R1 (cultivated with Oscillatoria sp.). Results showed that the mixed liquid suspended solid of mature HSP in R1 (2.17g/L) was larger than that in R2 (cultivated without Oscillatoria sp.) (2.03g/L), and the surface of HSP in R1 was smoother. Values of PN/PS in R1 (2.01) was slightly larger than R2 (1.45) on 30d, implying that inoculating Oscillatoria sp. enhanced the hydrophobicity of granular sludge. Furthermore, results showed that the interfacial free energy was strengthened in R1 with the potential energy barrier of 2616.75kT, which was lower than the potential energy barrier in R2 (5037.85kT). The EPS producing bacteria were enriched and the relative abundance of enzymes related to EPS production was increased by 8%~156% in HSP after being strengthened by Oscillatoria sp. Overall, inoculating filamentous cyanobacteria Oscillatoria sp. into activated sludge facilitated the attachment and aggregation between bacteria and microalgae in HSP, contributing to the rapid development of HSP.

, authors=Yu-chen AN, Bing ZHANG, Jia-wei FAN, Wen-xin SHI*, authorsList=Yu-chen AN, Bing ZHANG, Jia-wei FAN, Wen-xin SHI, authorCompany=null, correspAuthors=Wen-xin SHI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314917532541219205, articleId=1314917531815604611, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=静态产氧光颗粒的快速形成过程及自凝聚机理, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

为强化静态产氧光颗粒(HSP)的快速稳定形成,通过在HSP培养初期接种颤藻Oscillatoria sp.(一种典型丝状蓝藻)得出了一种强化HSP快速高效造粒的新方法;研究HSP的形成过程、形态特征、胞外聚合物(EPS)分泌、表面热力学特性以及微生物群落结构,分析该丝状蓝藻强化HSP造粒机理.研究表明,添加颤藻培养组的污泥生物量(2.17g/L)大于未添加颤藻培养组(2.03g/L),且颗粒表面形态更加光滑.在第30d,添加颤藻培养组中PN/PS为2.01,高于未添加颤藻培养组(1.45),表明污泥表面疏水性得到提高.此外,表面热力学分析结果表明接种颤藻培养组具有更强的界面自由能,其势能能垒(2616.75kT)低于未接种颤藻培养组(5037.85kT).经颤藻强化后,HSP中的EPS产生菌得到富集,与EPS分泌相关的功能酶相对丰度提高了8%~156%.在HSP培养初期接种丝状蓝藻(颤藻)促进了污泥中微生物与藻类附着生长与相互聚集,有效强化了HSP的快速稳定形成.

, authors=安宇晨, 张冰, 樊佳炜, 时文歆*, authorsList=安宇晨, 张冰, 樊佳炜, 时文歆, authorCompany=null, correspAuthors=时文歆, authorNote=

安宇晨(2000-),女,河北邯郸人,重庆大学硕士研究生,研究方向为污水生物处理技术.发表论文1篇..

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安宇晨(2000-),女,河北邯郸人,重庆大学硕士研究生,研究方向为污水生物处理技术.发表论文1篇..

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安宇晨(2000-),女,河北邯郸人,重庆大学硕士研究生,研究方向为污水生物处理技术.发表论文1篇..

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静态产氧光颗粒的快速形成过程及自凝聚机理
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安宇晨 , 张冰 , 樊佳炜 , 时文歆 *
中国环境科学 | 水污染与控制 2025,45(7): 3714-3723
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中国环境科学 |水污染与控制 2025 , 45 (7) : 3714 -3723
静态产氧光颗粒的快速形成过程及自凝聚机理
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安宇晨 , 张冰, 樊佳炜, 时文歆*
作者信息
  • 重庆大学环境与生态学院,三峡库区生态环境教育部重点实验室,重庆 400045
通讯作者:
* 责任作者,教授,
作者简介:

安宇晨(2000-),女,河北邯郸人,重庆大学硕士研究生,研究方向为污水生物处理技术.发表论文1篇..

Unveiling the mechanism for the rapid development of hydrostatically oxygenic photogranules
Yu-chen AN , Bing ZHANG, Jia-wei FAN, Wen-xin SHI*
Affiliations
  • Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400045, China
出版时间: 2025-07-20
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为强化静态产氧光颗粒(HSP)的快速稳定形成,通过在HSP培养初期接种颤藻Oscillatoria sp.(一种典型丝状蓝藻)得出了一种强化HSP快速高效造粒的新方法;研究HSP的形成过程、形态特征、胞外聚合物(EPS)分泌、表面热力学特性以及微生物群落结构,分析该丝状蓝藻强化HSP造粒机理.研究表明,添加颤藻培养组的污泥生物量(2.17g/L)大于未添加颤藻培养组(2.03g/L),且颗粒表面形态更加光滑.在第30d,添加颤藻培养组中PN/PS为2.01,高于未添加颤藻培养组(1.45),表明污泥表面疏水性得到提高.此外,表面热力学分析结果表明接种颤藻培养组具有更强的界面自由能,其势能能垒(2616.75kT)低于未接种颤藻培养组(5037.85kT).经颤藻强化后,HSP中的EPS产生菌得到富集,与EPS分泌相关的功能酶相对丰度提高了8%~156%.在HSP培养初期接种丝状蓝藻(颤藻)促进了污泥中微生物与藻类附着生长与相互聚集,有效强化了HSP的快速稳定形成.

菌藻共生  /  强化策略  /  形成机理  /  亲疏水性  /  XDLVO理论

In order to strengthen the rapid and stable formation of hydrostatically oxygenic photogranules (HSP), a strategy of inoculating Oscillatoria sp., a typical kind of filamentous cyanobacteria, into activated sludge at the early stage of cultivation was proposed. The photogranulation process, morphology, extracellular polymeric substances contents, surface thermodynamics properties and microbial community were analyzed to elucidate the mechanism for efficient photogranulation in R1 (cultivated with Oscillatoria sp.). Results showed that the mixed liquid suspended solid of mature HSP in R1 (2.17g/L) was larger than that in R2 (cultivated without Oscillatoria sp.) (2.03g/L), and the surface of HSP in R1 was smoother. Values of PN/PS in R1 (2.01) was slightly larger than R2 (1.45) on 30d, implying that inoculating Oscillatoria sp. enhanced the hydrophobicity of granular sludge. Furthermore, results showed that the interfacial free energy was strengthened in R1 with the potential energy barrier of 2616.75kT, which was lower than the potential energy barrier in R2 (5037.85kT). The EPS producing bacteria were enriched and the relative abundance of enzymes related to EPS production was increased by 8%~156% in HSP after being strengthened by Oscillatoria sp. Overall, inoculating filamentous cyanobacteria Oscillatoria sp. into activated sludge facilitated the attachment and aggregation between bacteria and microalgae in HSP, contributing to the rapid development of HSP.

algal-bacterial symbiotic  /  strengthening strategy  /  formation mechanism  /  hydrophilicity and hydrophobicity  /  extended Derjaguin-Landau-Verwey-Overbeek theory
安宇晨, 张冰, 樊佳炜, 时文歆. 静态产氧光颗粒的快速形成过程及自凝聚机理. 中国环境科学, 2025 , 45 (7) : 3714 -3723 .
Yu-chen AN, Bing ZHANG, Jia-wei FAN, Wen-xin SHI. Unveiling the mechanism for the rapid development of hydrostatically oxygenic photogranules[J]. China Environmental Science, 2025 , 45 (7) : 3714 -3723 .
  • 国家自然科学基金资助项目(52370029)
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2025年第45卷第7期
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  • 接收时间:2024-12-17
  • 首发时间:2026-10-08
  • 出版时间:2025-07-20
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  • 收稿日期:2024-12-17
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国家自然科学基金资助项目(52370029)
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    重庆大学环境与生态学院,三峡库区生态环境教育部重点实验室,重庆 400045

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2种不同金属材料的力学参数
科
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属数
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genus
种数
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species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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