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Feammox functional microorganisms were enriched from anaerobic digestion sludge with NH4+-N and Fe(Ⅲ) supplementation and their long-term nitrogen removal performance was evaluated. Feammox activity was detected on day 66and remained stable in subsequent batches. The relative abundances of the potential Feammox functional bacteria Geobacter and Anaeromyxobacter increased by 457-fold and 11.6-fold, respectively. Metagenomic analysis revealed a significant increase in the total relative abundance of functional genes associated with three nitrite transformation pathways: ammonia oxidation to nitrite (amo, hao), nitrite denitrification to nitrogen gas (nirSK, norB, nosCZ), and nitrite reduction to ammonium (nirABD, nrfAH). The addition of the electron shuttle AQDS promoted extracellular electron transfer and enhanced the ammonia removal efficiency by 17.8%.

, authors=Dan-yang ZHU1, Si-yuan DIAO2, Hui GONG1, Xiao-hu DAI1, authorsList=Dan-yang ZHU, Si-yuan DIAO, Hui GONG, Xiao-hu DAI, authorCompany=null, correspAuthors=Hui GONG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314917512437915912, articleId=1314917510747611398, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=厌氧铁氨氧化功能微生物的驯化与AQDS强化作用, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

利用厌氧消化污泥,通过添加含氨氮和Fe(Ⅲ)的配水富集厌氧铁氨氧化(Feammox)功能微生物,并评估其长期脱氮性能.结果表明,第66d出现Feammox活性,并在后续批次中保持稳定;Feammox潜在功能菌Geobacter和Anaeromyxobacter的相对丰度分别增加了457倍和11.6倍.宏基因组分析显示,3条与亚硝氮转化相关的途径功能基因总相对丰度显著提升,包括氨氮氧化为亚硝氮(amo、hao),亚硝氮反硝化为氮气(nirSK、norB、nosCZ)和亚硝氮还原为铵(nirABD、nrfAH).添加电子穿梭体蒽醌-2,6-二磺酸(AQDS)后可促进细胞外电子转移,氨氮去除率提高了17.8%.

, authors=朱丹阳1, 刁思圆2, 宫徽1, 戴晓虎1, authorsList=朱丹阳, 刁思圆, 宫徽, 戴晓虎, authorCompany=null, correspAuthors=宫徽, authorNote=

朱丹阳(2000-),女,四川广安人,同济大学博士研究生,主要从事厌氧铁氨氧化研究.发表论文3篇..

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* 责任作者,副教授,
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朱丹阳(2000-),女,四川广安人,同济大学博士研究生,主要从事厌氧铁氨氧化研究.发表论文3篇..

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朱丹阳(2000-),女,四川广安人,同济大学博士研究生,主要从事厌氧铁氨氧化研究.发表论文3篇..

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厌氧铁氨氧化功能微生物的驯化与AQDS强化作用
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朱丹阳 1 , 刁思圆 2 , 宫徽 1 , 戴晓虎 1
中国环境科学 | 水污染与控制 2025,45(7): 3735-3741
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中国环境科学 |水污染与控制 2025 , 45 (7) : 3735 -3741
厌氧铁氨氧化功能微生物的驯化与AQDS强化作用
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朱丹阳1 , 刁思圆2, 宫徽1 , 戴晓虎1
作者信息
  • 1.同济大学环境科学与工程学院,污染控制与资源化研究国家重点实验室,上海 200092
  • 2.上海电力大学环境与化学工程学院,上海 200090
通讯作者:
* 责任作者,副教授,
作者简介:

朱丹阳(2000-),女,四川广安人,同济大学博士研究生,主要从事厌氧铁氨氧化研究.发表论文3篇..

Enrichment of Feammox functional microorganisms and the strengthening effect of AQDS
Dan-yang ZHU1 , Si-yuan DIAO2, Hui GONG1 , Xiao-hu DAI1
Affiliations
  • 1.State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
  • 2.College of Environmental Science and Chemical Engineering, Shanghai University of Electric Power, Shanghai 201306, China
出版时间: 2025-07-20
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利用厌氧消化污泥,通过添加含氨氮和Fe(Ⅲ)的配水富集厌氧铁氨氧化(Feammox)功能微生物,并评估其长期脱氮性能.结果表明,第66d出现Feammox活性,并在后续批次中保持稳定;Feammox潜在功能菌Geobacter和Anaeromyxobacter的相对丰度分别增加了457倍和11.6倍.宏基因组分析显示,3条与亚硝氮转化相关的途径功能基因总相对丰度显著提升,包括氨氮氧化为亚硝氮(amo、hao),亚硝氮反硝化为氮气(nirSK、norB、nosCZ)和亚硝氮还原为铵(nirABD、nrfAH).添加电子穿梭体蒽醌-2,6-二磺酸(AQDS)后可促进细胞外电子转移,氨氮去除率提高了17.8%.

自养脱氮  /  厌氧铁氨氧化  /  AQDS  /  胞外电子传递  /  宏基因分析

Feammox functional microorganisms were enriched from anaerobic digestion sludge with NH4+-N and Fe(Ⅲ) supplementation and their long-term nitrogen removal performance was evaluated. Feammox activity was detected on day 66and remained stable in subsequent batches. The relative abundances of the potential Feammox functional bacteria Geobacter and Anaeromyxobacter increased by 457-fold and 11.6-fold, respectively. Metagenomic analysis revealed a significant increase in the total relative abundance of functional genes associated with three nitrite transformation pathways: ammonia oxidation to nitrite (amo, hao), nitrite denitrification to nitrogen gas (nirSK, norB, nosCZ), and nitrite reduction to ammonium (nirABD, nrfAH). The addition of the electron shuttle AQDS promoted extracellular electron transfer and enhanced the ammonia removal efficiency by 17.8%.

autotrophic nitrogen removal  /  feammox  /  AQDS  /  extracellular electron transfer  /  metagenomic analysis
朱丹阳, 刁思圆, 宫徽, 戴晓虎. 厌氧铁氨氧化功能微生物的驯化与AQDS强化作用. 中国环境科学, 2025 , 45 (7) : 3735 -3741 .
Dan-yang ZHU, Si-yuan DIAO, Hui GONG, Xiao-hu DAI. Enrichment of Feammox functional microorganisms and the strengthening effect of AQDS[J]. China Environmental Science, 2025 , 45 (7) : 3735 -3741 .
  • 国家自然科学基金资助项目(52470159)
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  • 接收时间:2024-12-23
  • 首发时间:2026-10-08
  • 出版时间:2025-07-20
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  • 收稿日期:2024-12-23
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国家自然科学基金资助项目(52470159)
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    1.同济大学环境科学与工程学院,污染控制与资源化研究国家重点实验室,上海 200092
    2.上海电力大学环境与化学工程学院,上海 200090

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2种不同金属材料的力学参数
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种数
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占总种数比例
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属
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species
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species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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