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Pseudomonas aeruginosa NY3, columnId=1240689595814302661, journalTitle=China Environmental Science, columnName=Environmental Microbiology, runingTitle=null, highlight=null, articleAbstract=
The catalytic degradation mechanism of petroleum hydrocarbons by Ferripyochelin (FerriPCH), formed by the combination of pyochelin (PCH) from the extracellular fluid of P. aeruginosa NY3 and Ferri, was investigated. The results showed that two chiral PCHs were produced by strain NY3. The purified PCH could degrade both alkanes and polycyclic aromatic compounds of petroleum hydrocarbon after combining with Ferri. A certain ratio of hexadecane, anthracene, as well as phenanthrene could be degraded by FerriPCH which formed by PCH and Ferri in the ratio from 2:1 to 1:1. In addition, degradation efficiency of aromatic hydrocarbons with low bio-availability was significantly higher than that of alkanes. Further studies showed that strongly oxidizing free radicals of •OH and •O2- produced after the binding of Ferri and PCH degraded petroleum hydrocarbons into carbon dioxide and water step by step.
, correspAuthors=Hong-yun NIE, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Zhi-ruo HUANG, Di-ni GUO, Tao ZHAO, Hong-yun NIE, Mai-qian NIE, Lei WANG, Bi-jie ZHAO), CN=ArticleExt(id=1241408726612104045, articleId=1241408718538068037, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=铜绿假单胞菌螯铁蛋白催化降解石油烃的机理, columnId=1240689596959346705, journalTitle=中国环境科学, columnName=环境微生物, runingTitle=null, highlight=null, articleAbstract=
探讨了铜绿假单胞菌Pseudomonas aeruginosa NY3分泌的螯铁蛋白(Pyochelin,PCH)与铁形成复合物(Ferripyochelin,FerriPCH)催化降解石油烃的作用机理.结果表明,菌株NY3分泌的2种PCH为手性对映体.当纯化后的PCH与Fe3+鳌合时,可降解石油烃中的烷烃和多环芳烃等污染物.当PCH与Fe3+的比例为2:1 ~ 1:1时,对十六烷,蒽和菲有一定的降解效果,且生物利用度低的芳香烃降解效率显著高于烷烃.研究发现,Fe3+与PCH鳌合会产生•OH和•O2-自由基,这些具有强氧化性能的自由基可将石油烃逐步降解为二氧化碳和水.
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黄芷若(2000-),女,湖北宜昌人,西安建筑科技大学硕士研究生,主要研究方向为难降解有机污染物的微生物处理技术.2391964534@qq.com.
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黄芷若(2000-),女,湖北宜昌人,西安建筑科技大学硕士研究生,主要研究方向为难降解有机污染物的微生物处理技术.2391964534@qq.com.
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Characterization of PCH by P. aeruginosa NY3, figureFileSmall=+YWfr4Vy5DeLFLYDMi2+Gg==, figureFileBig=7X6YvcZCTXEoE+9hakdmnw==, tableContent=null), ArticleFig(id=1241408732563820968, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=CN, label=图1, caption=
铜绿假单胞菌NY3分泌的PCH表征(a)PCH紫外-可见吸收光谱图;(b)PCH液相色谱图;(c)PCH正离子一级质谱图;(d)PCH正离子二级质谱图
, figureFileSmall=+YWfr4Vy5DeLFLYDMi2+Gg==, figureFileBig=7X6YvcZCTXEoE+9hakdmnw==, tableContent=null), ArticleFig(id=1241408732844839369, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=EN, label=Fig.2, caption=
Effect of FerriPCH on the degradation efficiency of hexadecane, figureFileSmall=/pqS2xOpE7cpskyo5LYjiQ==, figureFileBig=skZZa60P4aGeApc5fnMz5Q==, tableContent=null), ArticleFig(id=1241408733058748896, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=CN, label=图2, caption=
FerriPCH对十六烷降解效率的影响(a)PCH质量浓度对十六烷降解效率的影响;(b)Fe3+质量浓度对十六烷降解效率的影响;(c)H2O2浓度对FerriPCH降解十六烷效率的影响
, figureFileSmall=/pqS2xOpE7cpskyo5LYjiQ==, figureFileBig=skZZa60P4aGeApc5fnMz5Q==, tableContent=null), ArticleFig(id=1241408733255881195, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=EN, label=Fig.3, caption=
Effect of FerriPCH on the degradation feeiciency of anthracene and phenanthrene, figureFileSmall=4x5Kdovtd5h0d/3uw0qj6Q==, figureFileBig=c6ObiJW+DRzRs3AZLQBjZA==, tableContent=null), ArticleFig(id=1241408733411070457, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=CN, label=图3, caption=
FerriPCH对蒽和菲降解效率的影响(a)PCH质量浓度对蒽降解效率的影响;(b)PCH质量浓度对菲降解效率的影响
, figureFileSmall=4x5Kdovtd5h0d/3uw0qj6Q==, figureFileBig=c6ObiJW+DRzRs3AZLQBjZA==, tableContent=null), ArticleFig(id=1241408733553676804, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=EN, label=Fig.4, caption=
Degradation pathway of petroleum hydrocarbons by FerriPCH, figureFileSmall=mzhsaGbVQpBRUChKDKNdvQ==, figureFileBig=G3Rbu7J6kb4BsMYhDcQPXQ==, tableContent=null), ArticleFig(id=1241408733662728720, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=CN, label=图4, caption=
FerriPCH降解石油烃的途径, figureFileSmall=mzhsaGbVQpBRUChKDKNdvQ==, figureFileBig=G3Rbu7J6kb4BsMYhDcQPXQ==, tableContent=null), ArticleFig(id=1241408733780169239, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=EN, label=Fig.5, caption=
Radical signals in the Buffer system, figureFileSmall=yAraima607+4VgdVHGjxow==, figureFileBig=aiN3ZE9Z5tHAWgfYg1Q0/A==, tableContent=null), ArticleFig(id=1241408733939552805, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=CN, label=图5, caption=
缓冲液体系中的自由基信号, figureFileSmall=yAraima607+4VgdVHGjxow==, figureFileBig=aiN3ZE9Z5tHAWgfYg1Q0/A==, tableContent=null), ArticleFig(id=1241408734048604717, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=EN, label=Table 1, caption=
Intermediates produced in the degradation of petroleum hydrocarbons by FerriPCH
, figureFileSmall=null, figureFileBig=null, tableContent=
| 序号 | 石油烃 | 主要离子(m/z) | 结构式 | 名称 |
|---|
| 1 | 十六烷 | 146,131,103,89,75,73,61,59,45 |  | 正丁醇 |
| 2 | 174,159,143,128,114,100,86,73,56,45 |  | 4,5-二羟基-2-戊烯 |
| 3 | 174,159,144,130,86,73,58,45 |  | 2-甲基-3羟基戊烷 |
| 4 | 204,189,174,130,100,86,75,59,45 |  | 6-羟基己酸 |
| 5 | 188,173,158,144,102,73,59,45 |  | 2-甲基-3-羟基己烷 |
| 6 | 290,275,188,170,147,131,98,73 |  | 己二酸 |
| 7 | 328,313,201,132,117,73,55 |  | 棕榈酸 |
| 8 | 菲 | 247,172,147,129,75,73,55,45 |  | 琥珀酸 |
| 9 | 234,203,189,147,144,129,103,73 |  | 1,2-二羟基萘 |
| 10 | 306,216,176,147,128,102,73 |  | 1,2-二羟基-3,4-二氢萘 |
| 11 | 331,275,227,172,147,129,111,73 |  | 1-羟基-2-(3-羟基-1-丙烯基)-3,4-二氢萘 |
| 12 | 蒽 | 227,191,143,129,111,98,75,55,41 |  | 2-羧甲基-3-羟甲基-1,2-二氢萘 |
| 13 | 246,216,147,103,73,55 |  | 2-羧基-1,2-二氢萘 |
| 14 | 306,216,176,147,128,102,73 |  | 2,3-二羟基-1,2-二氢萘 |
| 15 | 208,180,152,126,76,50 |  | 9,10-蒽醌 |
), ArticleFig(id=1241408734191211061, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1241408718538068037, language=CN, label=表1, caption=
FerriPCH降解石油烃过程中产生的中间产物
, figureFileSmall=null, figureFileBig=null, tableContent=
| 序号 | 石油烃 | 主要离子(m/z) | 结构式 | 名称 |
|---|
| 1 | 十六烷 | 146,131,103,89,75,73,61,59,45 |  | 正丁醇 |
| 2 | 174,159,143,128,114,100,86,73,56,45 |  | 4,5-二羟基-2-戊烯 |
| 3 | 174,159,144,130,86,73,58,45 |  | 2-甲基-3羟基戊烷 |
| 4 | 204,189,174,130,100,86,75,59,45 |  | 6-羟基己酸 |
| 5 | 188,173,158,144,102,73,59,45 |  | 2-甲基-3-羟基己烷 |
| 6 | 290,275,188,170,147,131,98,73 |  | 己二酸 |
| 7 | 328,313,201,132,117,73,55 |  | 棕榈酸 |
| 8 | 菲 | 247,172,147,129,75,73,55,45 |  | 琥珀酸 |
| 9 | 234,203,189,147,144,129,103,73 |  | 1,2-二羟基萘 |
| 10 | 306,216,176,147,128,102,73 |  | 1,2-二羟基-3,4-二氢萘 |
| 11 | 331,275,227,172,147,129,111,73 |  | 1-羟基-2-(3-羟基-1-丙烯基)-3,4-二氢萘 |
| 12 | 蒽 | 227,191,143,129,111,98,75,55,41 |  | 2-羧甲基-3-羟甲基-1,2-二氢萘 |
| 13 | 246,216,147,103,73,55 |  | 2-羧基-1,2-二氢萘 |
| 14 | 306,216,176,147,128,102,73 |  | 2,3-二羟基-1,2-二氢萘 |
| 15 | 208,180,152,126,76,50 |  | 9,10-蒽醌 |
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