首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1234106390516659030, tenantId=1146029695717560320, journalId=1234093305789726721, issueId=1234106384963400440, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1734192000000, receivedDateStr=2024-12-15, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1772163492087, onlineDateStr=2026-02-27, pubDate=1750348800000, pubDateStr=2025-06-20, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772163492087, onlineIssueDateStr=2026-02-27, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772163492086, creator=13701087609, updateTime=1772163492086, updator=13701087609, issue=Issue{id=1234106384963400440, tenantId=1146029695717560320, journalId=1234093305789726721, year='2025', volume='45', issue='6', pageStart='2961', pageEnd='3552', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1772163490763, creator=13701087609, updateTime=1772163969484, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1234108392948682946, tenantId=1146029695717560320, journalId=1234093305789726721, issueId=1234106384963400440, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1234108392948682947, tenantId=1146029695717560320, journalId=1234093305789726721, issueId=1234106384963400440, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=3394, endPage=3401, ext={EN=ArticleExt(id=1234106390940283758, articleId=1234106390516659030, tenantId=1146029695717560320, journalId=1234093305789726721, language=EN, title=Remediation of contaminated soil with PAEs and Cd using an immobilized microbial agent, columnId=1234106386213294713, journalTitle=China Environmental Science, columnName=Agriculture and Country Emerging Contaminants, runingTitle=null, highlight=null, articleAbstract=

To address the co-contamination of phthalic acid esters (PAEs) and cadmium (Cd) in agricultural soils of Guangxi province, a novel approach using immobilized functional microbial agent has been proposed. A composite microbial consortium, composed of three functional bacterial strains including Gordonia sp., Rhodococcus sp., and Bacillus sp., was developed with the ability to tolerate Cd and degrade PAEs. The microbial agent was immobilized on a thiol-modified montmorillonite-biochar composite carrier with optimized preparation conditions to enhance their remediation capabilities. The synergistic remediation efficacy of the agent on PAEs-Cd co-contaminated soils and the underlying mechanisms were elucidated. The results demonstrated that the composite microbial consortium achieved a degradation rate of 92.7% for total PAEs within 5days, while the carrier material exhibited a Cd saturation adsorption capacity of 15.2mg/kg. The optimal immobilization conditions were determined to be 30℃, with a bacteria-to-carrier ratio of 1:20 (V/M) for 1day. Under these conditions, the immobilized microbial agent achieved a degradation rate of 95.4% for ΣPAEs within 5days. When applied at a dosage of 1% to PAEs-Cd co-contaminated soils, the immobilized microbial agent resulted in 54.14% PAEs elimination and 37.06% decrease of exchangeable Cd after 50days. The immobilized microbial agent exhibited favorable synergistic remediation efficacy for PAEs-Cd co-contamination. The research findings provided a theoretical basis for the remediation of PAEs-Cd co-contamination in farmland soil of Guangxi and filled the theoretical gap in the control and remediation of PAEs-Cd co-contamination.

, correspAuthors=Yan-zheng GAO, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Fan WANG, Wen-jie LI, Peng-fei MAO, Yan-zheng GAO), CN=ArticleExt(id=1234106397936382263, articleId=1234106390516659030, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=固定化菌剂对PAEs-Cd复合污染土壤的修复作用, columnId=1234106388545327751, journalTitle=中国环境科学, columnName=农业与农村新污染物, runingTitle=null, highlight=null, articleAbstract=

针对广西农田土壤中存在的邻苯二甲酸酯(PAEs)和镉(Cd)复合污染问题，提出了固定化功能菌剂修复复合污染农田土壤的思路.由Gordonia sp.、Rhodococcus sp.、Bacillus sp.等3种功能菌株构建出具备耐Cd和降解PAEs性能的复合菌群，以巯基改性蒙脱石-生物炭复合材料为固定化载体，制备了固定化菌剂，优化了其制备条件，揭示了菌剂施用对PAEs-Cd复合污染土壤的协同修复效能，探究功能菌剂的作用机制.结果表明，5d内复合菌群对6种优控PAEs的总降解率达92.7%，载体材料对Cd饱和吸附量达15.2mg/kg;菌剂最优固定化条件为30℃、菌料比1:20(V/M)下固定1d.制备的固定化菌剂5d内对ΣPAEs降解率达95.4%;以1%施用量投加固定化菌剂至PAEs-Cd复合污染土壤中，50d后土壤中PAEs消减率达54.14%，可交换态Cd减少37.06%，菌剂对PAEs-Cd复合污染有良好的协同修复效能.

, correspAuthors=高彦征, authorNote=null, correspAuthorsNote=
* 责任作者，教授，
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=VQ3KuMtmpWBFYGn8pSnX/g==, magXml=IE+F5qInI3EINbZerfT3CA==, pdfUrl=null, pdf=fLmsiwCfI+aecgCvJnB+mQ==, pdfFileSize=2251364, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=rEZFf/U8Lnpm+ZPaaFDWQQ==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=Cu5r4JZ7SZ1nVO90tac3wg==, mapNumber=null, authorCompany=null, fund=null, authors=

王凡(2000-)，男，江苏宿迁人，南京农业大学硕士研究生，主要从事土壤有机污染控制与修复研究..

, authorsList=王凡, 李文杰, 毛鹏飞, 高彦征)}, authors=[Author(id=1234106398557139323, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=2022103031@stu.njau.edu, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1234106398871712161, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106398557139323, language=EN, stringName=Fan WANG, firstName=Fan, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1234106399018512821, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106398557139323, language=CN, stringName=王凡, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800, bio={"content":"

王凡(2000-)，男，江苏宿迁人，南京农业大学硕士研究生，主要从事土壤有机污染控制与修复研究..

"}, bioImg=null, bioContent=

王凡(2000-)，男，江苏宿迁人，南京农业大学硕士研究生，主要从事土壤有机污染控制与修复研究..

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1234106398301286753, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, xref=null, ext=[AuthorCompanyExt(id=1234106398309675363, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China), AuthorCompanyExt(id=1234106398318063972, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800)])]), Author(id=1234106399299531214, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1234106399425360346, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106399299531214, language=EN, stringName=Wen-jie LI, firstName=Wen-jie, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1234106399580549612, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106399299531214, language=CN, stringName=李文杰, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1234106398301286753, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, xref=null, ext=[AuthorCompanyExt(id=1234106398309675363, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China), AuthorCompanyExt(id=1234106398318063972, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800)])]), Author(id=1234106399727350267, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1234106399857373710, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106399727350267, language=EN, stringName=Peng-fei MAO, firstName=Peng-fei, middleName=null, lastName=MAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1234106399983202852, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106399727350267, language=CN, stringName=毛鹏飞, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1234106398301286753, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, xref=null, ext=[AuthorCompanyExt(id=1234106398309675363, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China), AuthorCompanyExt(id=1234106398318063972, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800)])]), Author(id=1234106401497346615, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=gaoyanzheng@njau.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1234106401652535885, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106401497346615, language=EN, stringName=Yan-zheng GAO, firstName=Yan-zheng, middleName=null, lastName=GAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=*, address=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1234106401807725155, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, authorId=1234106401497346615, language=CN, stringName=高彦征, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=*, address=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1234106398301286753, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, xref=null, ext=[AuthorCompanyExt(id=1234106398309675363, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China), AuthorCompanyExt(id=1234106398318063972, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800)])])], keywords=[Keyword(id=1234106401979691645, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, orderNo=1, keyword=immobilized microbial agent), Keyword(id=1234106402092937871, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, orderNo=2, keyword=phthalic acid esters), Keyword(id=1234106402222961320, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, orderNo=3, keyword=cadmium), Keyword(id=1234106402327818934, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, orderNo=4, keyword=co-contamination), Keyword(id=1234106402503979722, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, orderNo=1, keyword=固定化菌剂), Keyword(id=1234106402671751907, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, orderNo=2, keyword=邻苯二甲酸酯), Keyword(id=1234106402805969646, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, orderNo=3, keyword=镉), Keyword(id=1234106402969547513, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, orderNo=4, keyword=复合污染)], refs=[Reference(id=1234106408808018049, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2011, volume=40, issue=6, pageStart=1695, pageEnd=1704, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=Shi J, Yu X, Zhang M, journalName=Journal of Environmental Quality, refType=null, unstructuredReference=Shi J，Yu X，Zhang M，et al. Potential risks of copper，zinc，and cadmium pollution due to pig manure application in a soil-rice system under intensive farming: a case study of Nanhu，China [J]. Journal of Environmental Quality，2011，40(6):1695-1704., articleTitle=Potential risks of copper，zinc，and cadmium pollution due to pig manure application in a soil-rice system under intensive farming: a case study of Nanhu，China, refAbstract=null), Reference(id=1234106408938041488, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2013, volume=180, issue=null, pageStart=265, pageEnd=273, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=Wang J, Luo Y, Teng Y, journalName=Environmental Pollution, refType=null, unstructuredReference=Wang J，Luo Y，Teng Y，et al. Soil contamination by phthalate esters in Chinese intensive vegetable production systems with different modes of use of plastic film [J]. Environmental Pollution，2013，180:265-273., articleTitle=Soil contamination by phthalate esters in Chinese intensive vegetable production systems with different modes of use of plastic film, refAbstract=null), Reference(id=1234106410401853597, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2013, volume=14, issue=5, pageStart=10197, pageEnd=10228, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=Olaniran A O, Balgobind A, Pillay B, journalName=International Journal of Molecular Sciences, refType=null, unstructuredReference=Olaniran A O，Balgobind A，Pillay B. Bioavailability of heavy metals in soil: impact on microbial biodegradation of organic compounds and possible improvement strategies [J]. International Journal of Molecular Sciences，2013，14(5):10197-10228., articleTitle=Bioavailability of heavy metals in soil: impact on microbial biodegradation of organic compounds and possible improvement strategies, refAbstract=null), Reference(id=1234106410590597295, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2020, volume=396, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=Rahman Z, journalName=Journal of Hazardous Materials, refType=null, unstructuredReference=Rahman Z. An overview on heavy metal resistant microorganisms for simultaneous treatment of multiple chemical pollutants at co- contaminated sites，and their multipurpose application [J]. Journal of Hazardous Materials，2020，396:122682., articleTitle=An overview on heavy metal resistant microorganisms for simultaneous treatment of multiple chemical pollutants at co- contaminated sites，and their multipurpose application, refAbstract=null), Reference(id=1234106410754175158, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2021, volume=38, issue=9, pageStart=867, pageEnd=876, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=Zhao S, Xu W, Zong S, journalName=Environmental Engineering Science, refType=null, unstructuredReference=Zhao S，Xu W，Zong S，et al. The pollution characteristics and full-scale risk assessment of coking plant soil co-contaminated with polycyclic aromatic hydrocarbons and heavy metals [J]. Environmental Engineering Science，2021，38(9):867-876., articleTitle=The pollution characteristics and full-scale risk assessment of coking plant soil co-contaminated with polycyclic aromatic hydrocarbons and heavy metals, refAbstract=null), Reference(id=1234106410921947330, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2004, volume=17, issue=null, pageStart=491, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=Aitio A, Tritscher A, journalName=Biometals, refType=null, unstructuredReference=Aitio A，Tritscher A. Effects on health of cadmium-WHO approaches and conclusions [J]. Biometals，2004，17:491., articleTitle=Effects on health of cadmium-WHO approaches and conclusions, refAbstract=null), Reference(id=1234106411030999245, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2020, volume=17, issue=11, pageStart=3782, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=Genchi G, Sinicropi M S, Lauria G, journalName=International Journal of Environmental Research and Public Health, refType=null, unstructuredReference=Genchi G，Sinicropi M S，Lauria G，et al. The effects of cadmium toxicity [J]. International Journal of Environmental Research and Public Health，2020，17(11):3782., articleTitle=The effects of cadmium toxicity, refAbstract=null), Reference(id=1234106411131662554, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2003, volume=53, issue=null, pageStart=583, pageEnd=591, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=Wilson S C, Meharg A A, journalName=Chemosphere, refType=null, unstructuredReference=Wilson S C，Meharg A A. Investigation of organic xenobiotic transfers，partitioning and processing in air-soil-plant systems using a microcosm apparatus. Part II: comparing the fate of chlorobenzenes in grass planted soil [J]. Chemosphere，2003，53:583-591., articleTitle=Investigation of organic xenobiotic transfers，partitioning and processing in air-soil-plant systems using a microcosm apparatus. Part II: comparing the fate of chlorobenzenes in grass planted soil, refAbstract=null), Reference(id=1234106411286851817, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2017, volume=171, issue=null, pageStart=621, pageEnd=645, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=Antoniadis V, Levizou E, Shaheen S M, journalName=Earth-Science Reviews, refType=null, unstructuredReference=Antoniadis V，Levizou E，Shaheen S M，et al. Trace elements in the soil-plant interface: Phytoavailability，translocation，and phytoremediation-A review [J]. Earth-Science Reviews，2017，171:621-645., articleTitle=Trace elements in the soil-plant interface: Phytoavailability，translocation，and phytoremediation-A review, refAbstract=null), Reference(id=1234106411437846776, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2015, volume=512, issue=null, pageStart=143, pageEnd=153, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=Chen H, Teng Y, Lu S, journalName=Science of The Total Environment, refType=null, unstructuredReference=Chen H，Teng Y，Lu S，et al. Contamination features and health risk of soil heavy metals in China [J]. Science of The Total Environment，2015，512:143-153., articleTitle=Contamination features and health risk of soil heavy metals in China, refAbstract=null), Reference(id=1234106411576258822, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2021, volume=64, issue=7, pageStart=1126, pageEnd=1139, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=Yang Q, Yang Z, Zhang Q, journalName=Science China Earth Sciences, refType=null, unstructuredReference=Yang Q，Yang Z，Zhang Q，et al. Ecological risk assessment of Cd and other heavy metals in soil-rice system in the karst areas with high geochemical background of Guangxi，China [J]. Science China Earth Sciences，2021，64(7):1126-1139., articleTitle=Ecological risk assessment of Cd and other heavy metals in soil-rice system in the karst areas with high geochemical background of Guangxi，China, refAbstract=null), Reference(id=1234106411794362646, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2020, volume=57, issue=5, pageStart=1137, pageEnd=1142, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=骆永明, 滕应, journalName=土壤学报, refType=null, unstructuredReference=骆永明，滕应.中国土壤污染与修复科技研究进展和展望[J]. 土壤学报，2020，57(5):1137-1142., articleTitle=中国土壤污染与修复科技研究进展和展望, refAbstract=null), Reference(id=1234106411953746220, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2020, volume=57, issue=5, pageStart=1137, pageEnd=1142, url=null, language=null, rfNumber=[12], rfOrder=12, authorNames=Luo Y M, Teng Y, journalName=Acta Pedologica Sinica, refType=null, unstructuredReference=Luo Y M，Teng Y. Progress and Prospects in Soil Pollution and Remediation Research in China [J]. Acta Pedologica Sinica，2020，57(5):1137-1142., articleTitle=Progress and Prospects in Soil Pollution and Remediation Research in China, refAbstract=null), Reference(id=1234106412100546880, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2018, volume=29, issue=6, pageStart=1583, pageEnd=1594, url=null, language=null, rfNumber=[13], rfOrder=13, authorNames=Deary M E, Ekumankama C C, Cummings S P, journalName=Land Degradation and Development, refType=null, unstructuredReference=Deary M E，Ekumankama C C，Cummings S P. Effect of lead，cadmium，and mercury co-contaminants on biodegradation in PAH-polluted soils [J]. Land Degradation and Development，2018，29(6):1583-1594., articleTitle=Effect of lead，cadmium，and mercury co-contaminants on biodegradation in PAH-polluted soils, refAbstract=null), Reference(id=1234106412247347533, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2002, volume=53, issue=3, pageStart=404, pageEnd=415, url=null, language=null, rfNumber=[14], rfOrder=14, authorNames=Otitoloju A A, journalName=Ecotoxicology and Environmental Safety, refType=null, unstructuredReference=Otitoloju A A. Evaluation of the joint-action toxicity of binary mixtures of heavy metals against the mangrove periwinkle Tympanotonus fuscatus var radula (L.) [J]. Ecotoxicology and Environmental Safety，2002，53(3):404-415., articleTitle=Evaluation of the joint-action toxicity of binary mixtures of heavy metals against the mangrove periwinkle Tympanotonus fuscatus var radula (L.), refAbstract=null), Reference(id=1234106412377370969, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2001, volume=12, issue=3, pageStart=469, pageEnd=473, url=null, language=null, rfNumber=[15], rfOrder=15, authorNames=Zheng Z, Zhou P, Wu Z, journalName=Journal of Applied Ecology, refType=null, unstructuredReference=Zheng Z，Zhou P，Wu Z. New advances in research of combined pollution [J]. Journal of Applied Ecology，2001，12(3):469-473., articleTitle=New advances in research of combined pollution, refAbstract=null), Reference(id=1234106412691943790, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2006, volume=65, issue=8, pageStart=1355, pageEnd=1361, url=null, language=null, rfNumber=[16], rfOrder=16, authorNames=Gao Y Z, Xiong W, Ling W T, journalName=Chemosphere, refType=null, unstructuredReference=Gao Y Z，Xiong W，Ling W T，et al. Sorption of phenanthrene by soils contaminated with heavy metals [J]. Chemosphere，2006，65(8):1355-1361., articleTitle=Sorption of phenanthrene by soils contaminated with heavy metals, refAbstract=null), Reference(id=1234106412863910268, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2022, volume=306, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=17, authorNames=Wang X, Wu H, Dai C, journalName=Environmental Pollution, refType=null, unstructuredReference=Wang X，Wu H，Dai C，et al. Microbial interactions enhanced environmental fitness and expanded ecological niches under dibutyl phthalate and cadmium co-contamination [J]. Environmental Pollution，2022，306:119362., articleTitle=Microbial interactions enhanced environmental fitness and expanded ecological niches under dibutyl phthalate and cadmium co-contamination, refAbstract=null), Reference(id=1234106412981350785, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=1975, volume=13, issue=null, pageStart=342, pageEnd=347, url=null, language=null, rfNumber=[18], rfOrder=18, authorNames=Engelhardt G, Wallnofer P R, Hutzinger O, journalName=Bulletin of Environmental Contamination and Toxicology, refType=null, unstructuredReference=Engelhardt G，Wallnofer P R，Hutzinger O，et al. The microbial metabolism of di-n-butyl phthalate and related dialkyl phthalates [J]. Bulletin of Environmental Contamination and Toxicology，1975，13:342-347., articleTitle=The microbial metabolism of di-n-butyl phthalate and related dialkyl phthalates, refAbstract=null), Reference(id=1234106413149122962, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2003, volume=309, issue=1, pageStart=36, pageEnd=43, url=null, language=null, rfNumber=[19], rfOrder=19, authorNames=Chatterjee S, Dutta T K, journalName=Biochemical and Biophysical Research Communications, refType=null, unstructuredReference=Chatterjee S，Dutta T K. Metabolism of butyl benzyl phthalate by Gordonia sp. strain MTCC 4818 [J]. Biochemical and Biophysical Research Communications，2003，309(1):36-43., articleTitle=Metabolism of butyl benzyl phthalate by Gordonia sp. strain MTCC 4818, refAbstract=null), Reference(id=1234106413337866655, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2003, volume=16, issue=2, pageStart=126, pageEnd=132, url=null, language=null, rfNumber=[20], rfOrder=20, authorNames=Wang J L, Ye Y C, Wu W Z, journalName=Biomedical and Environmental Sciences, refType=null, unstructuredReference=Wang J L，Ye Y C，Wu W Z. Comparison of di-n-methyl phthalate biodegradation by free and immobilized microbial cells [J]. Biomedical and Environmental Sciences，2003，16(2):126-132., articleTitle=Comparison of di-n-methyl phthalate biodegradation by free and immobilized microbial cells, refAbstract=null), Reference(id=1234106413467890091, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2010, volume=20, issue=10, pageStart=1440, pageEnd=1445, url=null, language=null, rfNumber=[21], rfOrder=21, authorNames=Jin D C, Liang R X, Dai Q Y, journalName=Journal of Microbiology and Biotechnology, refType=null, unstructuredReference=Jin D C，Liang R X，Dai Q Y，et al. Biodegradation of di-n-butyl phthalate by Rhodococcus sp. JDC-11and molecular detection of 3，4-phthalate dioxygenase gene [J]. Journal of Microbiology and Biotechnology，2010，20(10):1440-1445., articleTitle=Biodegradation of di-n-butyl phthalate by Rhodococcus sp. JDC-11and molecular detection of 3，4-phthalate dioxygenase gene, refAbstract=null), Reference(id=1234106414935896503, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2022, volume=204, issue=null, pageStart=112, pageEnd=136, url=null, language=null, rfNumber=[22], rfOrder=22, authorNames=Priyanka J V, Rajalakshmi S, Kumar P S, journalName=Environmental Research, refType=null, unstructuredReference=Priyanka J V，Rajalakshmi S，Kumar P S，et al. Bioremediation of soil contaminated with toxic mixed reactive azo dyes by co-cultured cells of Enterobacter cloacae and Bacillus subtilis [J]. Environmental Research，2022，204:112-136., articleTitle=Bioremediation of soil contaminated with toxic mixed reactive azo dyes by co-cultured cells of Enterobacter cloacae and Bacillus subtilis, refAbstract=null), Reference(id=1234106415133028812, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2017, volume=8, issue=1, pageStart=1089, pageEnd=null, url=null, language=null, rfNumber=[23], rfOrder=23, authorNames=Hagemann N, Joseph S, Schmidt H P, journalName=Nature communications, refType=null, unstructuredReference=Hagemann N，Joseph S，Schmidt H P，et al. Organic coating on biochar explains its nutrient retention and stimulation of soil fertility [J]. Nature communications，2017，8(1):1089., articleTitle=Organic coating on biochar explains its nutrient retention and stimulation of soil fertility, refAbstract=null), Reference(id=1234106415279829472, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2021, volume=55, issue=22, pageStart=15256, pageEnd=15265, url=null, language=null, rfNumber=[24], rfOrder=24, authorNames=Guo D, Xia Q, Zeng Q, journalName=Environmental Science and Technology, refType=null, unstructuredReference=Guo D，Xia Q，Zeng Q，et al. Antibacterial mechanisms of reduced iron-containing smectite–illite clay minerals [J]. Environmental Science and Technology，2021，55(22):15256-15265., articleTitle=Antibacterial mechanisms of reduced iron-containing smectite–illite clay minerals, refAbstract=null), Reference(id=1234106415401464301, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2021, volume=55, issue=3, pageStart=1650, pageEnd=1658, url=null, language=null, rfNumber=[25], rfOrder=25, authorNames=Van G N, Christl I, Kretzschmar R, journalName=Environmental Science and Technology, refType=null, unstructuredReference=Van G N，Christl I，Kretzschmar R，et al. The effect of aeration on Mn(II) sorbed to clay minerals and its impact on Cd retention [J]. Environmental Science and Technology，2021，55(3):1650-1658., articleTitle=The effect of aeration on Mn(II) sorbed to clay minerals and its impact on Cd retention, refAbstract=null), Reference(id=1234106415544070647, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2017, volume=140, issue=null, pageStart=37, pageEnd=47, url=null, language=null, rfNumber=[26], rfOrder=26, authorNames=Abbas T, Rizwan M, Ali S, journalName=Ecotoxicology and Environmental Safety, refType=null, unstructuredReference=Abbas T，Rizwan M，Ali S，et al. Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in a soil with aged contamination [J]. Ecotoxicology and Environmental Safety，2017，140:37-47., articleTitle=Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in a soil with aged contamination, refAbstract=null), Reference(id=1234106415707648516, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2009, volume=42, issue=3/4, pageStart=410, pageEnd=414, url=null, language=null, rfNumber=[27], rfOrder=27, authorNames=Guimarães A M, Ciminelli V S, Vasconcelos W L, journalName=Applied Clay Science, refType=null, unstructuredReference=Guimarães A M，Ciminelli V S，Vasconcelos W L，et al. Smectite organofunctionalized with thiol groups for adsorption of heavy metal ions [J]. Applied Clay Science，2009，42(3/4):410-414., articleTitle=Smectite organofunctionalized with thiol groups for adsorption of heavy metal ions, refAbstract=null), Reference(id=1234106415904780824, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2018, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[28], rfOrder=28, authorNames=李静, journalName=null, refType=null, unstructuredReference=李静.邻苯二甲酸酯降解菌的降解特性与降解机制的初步研究[D]. 重庆:西南大学，2018., articleTitle=邻苯二甲酸酯降解菌的降解特性与降解机制的初步研究, refAbstract=null), Reference(id=1234106416038998562, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2018, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[28], rfOrder=29, authorNames=Li J, journalName=null, refType=null, unstructuredReference=Li J. Preliminary study on the degrading charicteristics and mechanisms of phthalic acid esters-degrading bacteria [D]. Chongqing: Southwest University，2018, articleTitle=Preliminary study on the degrading charicteristics and mechanisms of phthalic acid esters-degrading bacteria, refAbstract=null), Reference(id=1234106416194187826, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2007, volume=244, issue=3, pageStart=511, pageEnd=517, url=null, language=null, rfNumber=[29], rfOrder=30, authorNames=Yates G T, Smotzer T, journalName=Journal of Theoretical Biology, refType=null, unstructuredReference=Yates G T，Smotzer T. On the lag phase and initial decline of microbial growth curves [J]. Journal of Theoretical Biology，2007，244(3):511-517., articleTitle=On the lag phase and initial decline of microbial growth curves, refAbstract=null), Reference(id=1234106416332599875, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2024, volume=44, issue=3, pageStart=1584, pageEnd=1591, url=null, language=null, rfNumber=[30], rfOrder=31, authorNames=陆雯逸, 王泽铭, 左翔之, journalName=中国环境科学, refType=null, unstructuredReference=陆雯逸，王泽铭，左翔之，等.固定化菌剂对邻苯二甲酸酯的降解效能及途径[J]. 中国环境科学，2024，44(3):1584-1591., articleTitle=固定化菌剂对邻苯二甲酸酯的降解效能及途径, refAbstract=null), Reference(id=1234106416475206222, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2024, volume=44, issue=3, pageStart=1584, pageEnd=1591, url=null, language=null, rfNumber=[30], rfOrder=32, authorNames=Lu W Y, Wang Z M, Zuo X Z, journalName=China Environmental Science, refType=null, unstructuredReference=Lu W Y，Wang Z M，Zuo X Z，et al. The degradation efficiency and pathways of phthalic acid esters by immobilized bacterial agent [J]. China Environmental Science，2024，44(3):1584-1591., articleTitle=The degradation efficiency and pathways of phthalic acid esters by immobilized bacterial agent, refAbstract=null), Reference(id=1234106416642978387, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2021, volume=37, issue=1, pageStart=223, pageEnd=null, url=null, language=null, rfNumber=[31], rfOrder=33, authorNames=张小红, 陶红, 王亚娟, journalName=生物技术通报, refType=null, unstructuredReference=张小红，陶红，王亚娟，等.固定化微球降解土壤中PAEs效果及影响因素[J]. 生物技术通报，2021，37(1):223., articleTitle=固定化微球降解土壤中PAEs效果及影响因素, refAbstract=null), Reference(id=1234106416869470821, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2021, volume=37, issue=1, pageStart=223, pageEnd=233, url=null, language=null, rfNumber=[31], rfOrder=34, authorNames=Zhang X H, Tao H, Wang Y J, journalName=Biotechnology Bulletin, refType=null, unstructuredReference=Zhang X H，Tao H，Wang Y J，et al. Effect and influencing factors of immobilized microspheres on degradation of phthalate esters in soil [J]. Biotechnology Bulletin，2021，37(1):223-233., articleTitle=Effect and influencing factors of immobilized microspheres on degradation of phthalate esters in soil, refAbstract=null), Reference(id=1234106417033048686, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2024, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[32], rfOrder=35, authorNames=褚美函, journalName=null, refType=null, unstructuredReference=褚美函.固定化菌剂对复合污染土壤的修复效果及微生物群落结构的影响[D]. 沈阳:沈阳大学，2024., articleTitle=固定化菌剂对复合污染土壤的修复效果及微生物群落结构的影响, refAbstract=null), Reference(id=1234106417276318329, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2024, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[32], rfOrder=36, authorNames=Chu M H, journalName=null, refType=null, unstructuredReference=Chu M H. The remediation effect of immobilized microbial agents on co-contaminated soils and their impact on microbial community structure [D]. Shenyang: Shenyang University，2024., articleTitle=The remediation effect of immobilized microbial agents on co-contaminated soils and their impact on microbial community structure, refAbstract=null), Reference(id=1234106417402147466, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2022, volume=290, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[33], rfOrder=37, authorNames=Zhao H, Li P, He X, journalName=Chemosphere, refType=null, unstructuredReference=Zhao H，Li P，He X，et al. Remediation of cadmium contaminated soil by modified gangue material: characterization，performance and mechanisms [J]. Chemosphere，2022，290:133347., articleTitle=Remediation of cadmium contaminated soil by modified gangue material: characterization，performance and mechanisms, refAbstract=null), Reference(id=1234106417527976598, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, doi=null, pmid=null, pmcid=null, year=2018, volume=194, issue=null, pageStart=579, pageEnd=587, url=null, language=null, rfNumber=[34], rfOrder=38, authorNames=Bashir S, Zhu J, Fu Q, journalName=Chemosphere, refType=null, unstructuredReference=Bashir S，Zhu J，Fu Q，et al. Cadmium mobility，uptake and anti-oxidative response of water spinach (Ipomoea aquatic) under rice straw biochar，zeolite and rock phosphate as amendments [J]. Chemosphere，2018，194:579-587., articleTitle=Cadmium mobility，uptake and anti-oxidative response of water spinach (Ipomoea aquatic) under rice straw biochar，zeolite and rock phosphate as amendments, refAbstract=null)], funds=[Fund(id=1234106408355033181, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, awardId=U22A20590; 42430703, language=CN, fundingSource=国家自然科学基金资助项目(U22A20590; 42430703), fundOrder=null, country=null), Fund(id=1234106408501833826, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, awardId=2023YFE0110800, language=CN, fundingSource=国家重点研发计划项目(2023YFE0110800), fundOrder=null, country=null), Fund(id=1234106408640245870, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, awardId=CX（24）1002, language=CN, fundingSource=江苏省农业科技自主创新资金(CX（24）1002), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1234106398301286753, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, xref=null, ext=[AuthorCompanyExt(id=1234106398309675363, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China), AuthorCompanyExt(id=1234106398318063972, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, companyId=1234106398301286753, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800)])], figs=[ArticleFig(id=1234106403145708302, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.1, caption=Degradation ability of cadmium tolerant domesticated strains on PAEs, figureFileSmall=6QXxI2CRsxOjtBNyOTMOZg==, figureFileBig=rEZFf/U8Lnpm+ZPaaFDWQQ==, tableContent=null), ArticleFig(id=1234106403288314652, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图1, caption=耐Cd驯化菌株PAEs降解效能, figureFileSmall=6QXxI2CRsxOjtBNyOTMOZg==, figureFileBig=rEZFf/U8Lnpm+ZPaaFDWQQ==, tableContent=null), ArticleFig(id=1234106403577721656, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.2, caption=The degradation efficiency of PAEs by the mixed microbial consortium, figureFileSmall=mh/kd+1+z6E6hUrDA2GlAg==, figureFileBig=omFxM2DKr8beClWvrDUIUw==, tableContent=null), ArticleFig(id=1234106403686773571, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图2, caption=复配菌群PAEs降解效能, figureFileSmall=mh/kd+1+z6E6hUrDA2GlAg==, figureFileBig=omFxM2DKr8beClWvrDUIUw==, tableContent=null), ArticleFig(id=1234106403812602705, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.3, caption=SEM morphology images of biochar and composite materials, figureFileSmall=0rR3+adMGBZKK2Lpal+iSg==, figureFileBig=vCvMVTdgS91DMqy40wk/mg==, tableContent=null), ArticleFig(id=1234106403946820447, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图3, caption=生物炭和复合材料的SEM形貌图, figureFileSmall=0rR3+adMGBZKK2Lpal+iSg==, figureFileBig=vCvMVTdgS91DMqy40wk/mg==, tableContent=null), ArticleFig(id=1234106404106204013, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.4, caption=FT-IR spectra of biochar and composite materials, figureFileSmall=3FrjBpxDcrDusSlSA2Sh5Q==, figureFileBig=lq5fz9MyvhG/e5bhWd/MWQ==, tableContent=null), ArticleFig(id=1234106404240421753, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图4, caption=生物炭和复合材料的FT-IR图, figureFileSmall=3FrjBpxDcrDusSlSA2Sh5Q==, figureFileBig=lq5fz9MyvhG/e5bhWd/MWQ==, tableContent=null), ArticleFig(id=1234106404408193931, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.5, caption=Cd deactivation performance of different carrier materials, figureFileSmall=Ju+EWOWOFifbVay0btPbRQ==, figureFileBig=hmKnMcdviCENS7298Ng8rg==, tableContent=null), ArticleFig(id=1234106404571771802, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图5, caption=复合载体材料的Cd饱和吸附曲线, figureFileSmall=Ju+EWOWOFifbVay0btPbRQ==, figureFileBig=hmKnMcdviCENS7298Ng8rg==, tableContent=null), ArticleFig(id=1234106406048166824, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.6, caption=SEM spectra of immobilized microbial agent, figureFileSmall=TE5RDs0NVKs7yYEQy9U55Q==, figureFileBig=w16mmnZeICLvsDVVAnpOiQ==, tableContent=null), ArticleFig(id=1234106406207550390, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图6, caption=固定化菌剂扫描电镜图, figureFileSmall=TE5RDs0NVKs7yYEQy9U55Q==, figureFileBig=w16mmnZeICLvsDVVAnpOiQ==, tableContent=null), ArticleFig(id=1234106406345962438, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.7, caption=Optimization of preparation conditions for immobilized microbial agents, figureFileSmall=uzOyjY3RINkuVrmw0pOa0w==, figureFileBig=r7YlCcqdTTQvUD82jVgjWA==, tableContent=null), ArticleFig(id=1234106406492763096, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图7, caption=固定化菌剂制备条件优化

不同字母表示不同处理间差异显著(P<0.05)

, figureFileSmall=uzOyjY3RINkuVrmw0pOa0w==, figureFileBig=r7YlCcqdTTQvUD82jVgjWA==, tableContent=null), ArticleFig(id=1234106406668923875, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.8, caption=The PAEs degradation performance of microbial agents under the optimal immobilization conditions, figureFileSmall=tgSGcBmYSRReHDnoWCCrbQ==, figureFileBig=vy1xbBsCadAVd0BLKuwRTg==, tableContent=null), ArticleFig(id=1234106406819918833, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图8, caption=最佳固定条件菌剂的PAEs降解效能, figureFileSmall=tgSGcBmYSRReHDnoWCCrbQ==, figureFileBig=vy1xbBsCadAVd0BLKuwRTg==, tableContent=null), ArticleFig(id=1234106407021245436, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.9, caption=The degradation curve of PAEs by microbial agents and the quasi-first-order kinetic fitting curve, figureFileSmall=FzsMiXExp8jhpxIUA5qNGw==, figureFileBig=sgVeiG5oimLtLYWXZ4zPZg==, tableContent=null), ArticleFig(id=1234106407180627971, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图9, caption=菌剂对PAEs的降解曲线和准一级动力学拟合曲线, figureFileSmall=FzsMiXExp8jhpxIUA5qNGw==, figureFileBig=sgVeiG5oimLtLYWXZ4zPZg==, tableContent=null), ArticleFig(id=1234106407331622924, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Fig.10, caption=The synergistic remediation effect of microbial agents on composite contaminated soil, figureFileSmall=BcyvLxUl6pqMokySNLPmCQ==, figureFileBig=WeZmPY5CCXhsiIFJR9NkKw==, tableContent=null), ArticleFig(id=1234106407478423575, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=图10, caption=菌剂对复合污染土壤协同修复作用, figureFileSmall=BcyvLxUl6pqMokySNLPmCQ==, figureFileBig=WeZmPY5CCXhsiIFJR9NkKw==, tableContent=null), ArticleFig(id=1234106407608447014, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Table 1, caption=

Basic physicochemical properties of the tested soil

, figureFileSmall=null, figureFileBig=null, tableContent=
指标棕色石灰土
黏粒(%)2.06±0.071
粉粒(%)13.08±0.37
砂粒(%)84.86±0.30
有机碳(g/kg)27.42±1.47
全氮(g/kg)2.63±0.39
ΣPAEs(mg/kg)2.56±0.26
全Cd(mg/kg)2.01±0.12
有效Cd(mg/kg)0.63±0.13
), ArticleFig(id=1234106407847522357, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=表1, caption=

供试土壤基础理化性质

, figureFileSmall=null, figureFileBig=null, tableContent=
指标棕色石灰土
黏粒(%)2.06±0.071
粉粒(%)13.08±0.37
砂粒(%)84.86±0.30
有机碳(g/kg)27.42±1.47
全氮(g/kg)2.63±0.39
ΣPAEs(mg/kg)2.56±0.26
全Cd(mg/kg)2.01±0.12
有效Cd(mg/kg)0.63±0.13
), ArticleFig(id=1234106407994323013, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=EN, label=Table 2, caption=

Degradation kinetic equation of PAEs

, figureFileSmall=null, figureFileBig=null, tableContent=
底物动力学方程半衰期(d)R2
DMPy=0.12433x+0.974392.230.907
DEPy=0.18349x+1.38182.320.982
DBPy=0.96563x+0.332975.780.966
BBPy=0.8416x+0.405333.780.842
DEHPy=0.90028x+0.257263.660.900
DnOPy=0.8712x+0.565464.450.871
), ArticleFig(id=1234106408170483791, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1234106390516659030, language=CN, label=表2, caption=

PAEs的降解动力学方程

, figureFileSmall=null, figureFileBig=null, tableContent=
底物动力学方程半衰期(d)R2
DMPy=0.12433x+0.974392.230.907
DEPy=0.18349x+1.38182.320.982
DBPy=0.96563x+0.332975.780.966
BBPy=0.8416x+0.405333.780.842
DEHPy=0.90028x+0.257263.660.900
DnOPy=0.8712x+0.565464.450.871
)], attaches=null, journal=Journal(id=1234092555462295552, delFlag=0, nameCn=中国环境科学, nameEn=China Environmental Science, nameHistory1=null, nameHistory2=null, issn=1000-6923, eissn=null, cn=11-2201/X, coden=ZHKEEI, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=fUkXgpzwRiw9vs+0dX4h8g==, journalPrice=null, startedYear=null, abbrevIsoEn=China Environmental Science, journalRemark=null, publicationField=null, createdTime=1772160193557, updatedTime=1772160729300, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Engineering, subjectName=null, subjectCodeEn=Engineering, subjectNameEn=null, picCn=fUkXgpzwRiw9vs+0dX4h8g==, picEn=w8+EIm00c59F/qhCr1EFJw==, jcr=null, cjcr=null, exts=[JournalExt(id=1234094802610999917, language=CN, name=中国环境科学, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1772160729315, updatedTime=1772160729315, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zghjkxauthor.manuscriptcloud.com/, submissionEditorUrl=https://zghjkxeditor.manuscriptcloud.com/, submissionReviewUrl=https://zghjkxauthor.manuscriptcloud.com/, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1234094802665525870, language=EN, name=China Environmental Science, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1772160729328, updatedTime=1772160729328, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zghjkxauthor.manuscriptcloud.com/, submissionEditorUrl=https://zghjkxeditor.manuscriptcloud.com/, submissionReviewUrl=https://zghjkxauthor.manuscriptcloud.com/, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1234093305789726721, websiteList=[Website(id=1234095050196578613, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1234093305789726721, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zghjkx/CN, language=CN, createTime=1772160788344, createBy=18614031015, updateTime=1772160813480, updateBy=18614031015, name=中国环境科学-中文, tplId=1146099689490845704, title=中国环境科学, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1234097146769756836, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=articleTextType, value=kx, createTime=1772161288206, updateTime=1772161288206, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146748785313, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=banner, value=null, createTime=1772161288201, updateTime=1772161288201, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146786534055, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=grayFlag, value=0, createTime=1772161288210, updateTime=1772161288210, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146740396704, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=logo, value=https://castjournals.cast.org.cn/joweb/zghjkx/CN/file/pic?fileId=MkE5LKk3Qw7XuYcjPisdew==, createTime=1772161288199, updateTime=1772161288199, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146799116969, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=minRunFlag, value=0, createTime=1772161288213, updateTime=1772161288213, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146761368227, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zghjkx/CN/file/pic, createTime=1772161288204, updateTime=1772161288204, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146794922664, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=silenceFlag, value=0, createTime=1772161288212, updateTime=1772161288212, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146757173922, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1772161288203, updateTime=1772161288203, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146773951141, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=themeColor, value=null, createTime=1772161288207, updateTime=1772161288207, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097146782339750, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050196578613, code=themeStyle, value=null, createTime=1772161288209, updateTime=1772161288209, creator=18614031015, updator=18614031015)]), Website(id=1234095050309824825, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1234093305789726721, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zghjkx/EN, language=EN, createTime=1772160788371, createBy=18614031015, updateTime=1772160830384, updateBy=18614031015, name=中国环境科学-英文, tplId=1146101810881728533, title=China Environmental Science, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1234097176519955118, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=articleTextType, value=kx, createTime=1772161295299, updateTime=1772161295299, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176494789291, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=banner, value=null, createTime=1772161295293, updateTime=1772161295293, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176536732337, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=grayFlag, value=0, createTime=1772161295303, updateTime=1772161295303, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176486400682, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=logo, value=https://castjournals.cast.org.cn/joweb/zghjkx/EN/file/pic?fileId=MkE5LKk3Qw7XuYcjPisdew==, createTime=1772161295291, updateTime=1772161295291, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176545120947, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=minRunFlag, value=0, createTime=1772161295305, updateTime=1772161295305, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176511566509, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zghjkx/EN/file/pic, createTime=1772161295297, updateTime=1772161295297, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176540926642, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=silenceFlag, value=0, createTime=1772161295304, updateTime=1772161295304, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176503177900, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1772161295295, updateTime=1772161295295, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176524149423, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=themeColor, value=null, createTime=1772161295300, updateTime=1772161295300, creator=18614031015, updator=18614031015), WebsiteProps(id=1234097176528343728, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1234095050309824825, code=themeStyle, value=null, createTime=1772161295301, updateTime=1772161295301, creator=18614031015, updator=18614031015)])], journalTitle=中国环境科学, weixinUrl=null, journalUrl=http://www.zghjkx.com.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=China Environmental Science, journalPhotoCn=fUkXgpzwRiw9vs+0dX4h8g==, journalPhotoEn=w8+EIm00c59F/qhCr1EFJw==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/zghjkx/CN/Y2025/V45/I6/3394, detailUrlEn=https://castjournals.cast.org.cn/joweb/zghjkx/EN/Y2025/V45/I6/3394, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zghjkx/CN/PDF/Y2025/V45/I6/3394, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zghjkx/EN/PDF/Y2025/V45/I6/3394, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
固定化菌剂对PAEs-Cd复合污染土壤的修复作用
收藏切换
PDF下载
王凡 , 李文杰 , 毛鹏飞 , 高彦征 *
中国环境科学 | 农业与农村新污染物 2025,45(6): 3394-3401
收起
收藏切换
中国环境科学 | 农业与农村新污染物 2025, 45(6): 3394-3401
固定化菌剂对PAEs-Cd复合污染土壤的修复作用
全屏
王凡 , 李文杰, 毛鹏飞, 高彦征*
作者信息
  • 南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800

    • 王凡(2000-)，男，江苏宿迁人，南京农业大学硕士研究生，主要从事土壤有机污染控制与修复研究..

通讯作者:

* 责任作者，教授，
Remediation of contaminated soil with PAEs and Cd using an immobilized microbial agent
Fan WANG , Wen-jie LI, Peng-fei MAO, Yan-zheng GAO*
Affiliations
  • Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University, Nanjing 211800, China
出版时间: 2025-06-20
文章导航
收藏切换
摘要
收起

针对广西农田土壤中存在的邻苯二甲酸酯(PAEs)和镉(Cd)复合污染问题，提出了固定化功能菌剂修复复合污染农田土壤的思路.由Gordonia sp.、Rhodococcus sp.、Bacillus sp.等3种功能菌株构建出具备耐Cd和降解PAEs性能的复合菌群，以巯基改性蒙脱石-生物炭复合材料为固定化载体，制备了固定化菌剂，优化了其制备条件，揭示了菌剂施用对PAEs-Cd复合污染土壤的协同修复效能，探究功能菌剂的作用机制.结果表明，5d内复合菌群对6种优控PAEs的总降解率达92.7%，载体材料对Cd饱和吸附量达15.2mg/kg;菌剂最优固定化条件为30℃、菌料比1:20(V/M)下固定1d.制备的固定化菌剂5d内对ΣPAEs降解率达95.4%;以1%施用量投加固定化菌剂至PAEs-Cd复合污染土壤中，50d后土壤中PAEs消减率达54.14%，可交换态Cd减少37.06%，菌剂对PAEs-Cd复合污染有良好的协同修复效能.

固定化菌剂  /  邻苯二甲酸酯  /  镉  /  复合污染

To address the co-contamination of phthalic acid esters (PAEs) and cadmium (Cd) in agricultural soils of Guangxi province, a novel approach using immobilized functional microbial agent has been proposed. A composite microbial consortium, composed of three functional bacterial strains including Gordonia sp., Rhodococcus sp., and Bacillus sp., was developed with the ability to tolerate Cd and degrade PAEs. The microbial agent was immobilized on a thiol-modified montmorillonite-biochar composite carrier with optimized preparation conditions to enhance their remediation capabilities. The synergistic remediation efficacy of the agent on PAEs-Cd co-contaminated soils and the underlying mechanisms were elucidated. The results demonstrated that the composite microbial consortium achieved a degradation rate of 92.7% for total PAEs within 5days, while the carrier material exhibited a Cd saturation adsorption capacity of 15.2mg/kg. The optimal immobilization conditions were determined to be 30℃, with a bacteria-to-carrier ratio of 1:20 (V/M) for 1day. Under these conditions, the immobilized microbial agent achieved a degradation rate of 95.4% for ΣPAEs within 5days. When applied at a dosage of 1% to PAEs-Cd co-contaminated soils, the immobilized microbial agent resulted in 54.14% PAEs elimination and 37.06% decrease of exchangeable Cd after 50days. The immobilized microbial agent exhibited favorable synergistic remediation efficacy for PAEs-Cd co-contamination. The research findings provided a theoretical basis for the remediation of PAEs-Cd co-contamination in farmland soil of Guangxi and filled the theoretical gap in the control and remediation of PAEs-Cd co-contamination.

immobilized microbial agent  /  phthalic acid esters  /  cadmium  /  co-contamination
王凡, 李文杰, 毛鹏飞, 高彦征. 固定化菌剂对PAEs-Cd复合污染土壤的修复作用. 中国环境科学, 2025 , 45 (6) : 3394 -3401 .
Fan WANG, Wen-jie LI, Peng-fei MAO, Yan-zheng GAO. Remediation of contaminated soil with PAEs and Cd using an immobilized microbial agent[J]. China Environmental Science, 2025 , 45 (6) : 3394 -3401 .
正文
收起
邻苯二甲酸酯类(PAEs)塑化剂和重金属镉(Cd)复合污染是我国污染土壤中常见的高风险污染类型，广受社会关注[1-2].复合污染是土壤污染的主要存在形式，美国环境保护署(EPA)优先控制清单中的高危污染场地有40%均检出有机污染物和重金属复合污染[3-4].PAEs-Cd复合污染对人体神经、呼吸、生殖等多个系统具备毒性[5-7].由于PAEs的亲脂性和Cd的水溶性，PAEs-Cd复合污染极易在蔬菜作物中积累[8-9].由于农业地膜广泛应用，桂林13个监测村的土壤样品中均检测出PAEs污染，ΣPAEs浓度为0.763~ 2.714mg/kg[10].在广西岩溶区，Cd和Ca离子半径相近，易发生同晶替代，Cd背景值高达1.004mg/kg，是全国表层土壤Cd平均值(0.29mg/kg)的数倍[11], PAEs-Cd复合污染严重威胁岩溶区农业安全生产和人群健康.
土壤有毒有机物-重金属复合污染过程与控制原理是国际环境领域研究的一个瓶颈[12-13].土壤复合污染不是污染效应的简单叠加，而是污染物之间发生协同、拮抗等相互作用[14-15]，本课题组早期研究表明，重金属污染后土壤中多环芳烃(菲)的吸附系数增大[16]，使环境污染效应更加复杂，治理难度更高.微生物修复是治理农田土壤PAEs-Cd复合污染的经济有效的途径，如Wang等[17]添加外源性菌株至DBP-Cd复合污染土壤，发现该菌株对DBP的代谢产物还可以吸附Cd.自1975年首次报道PAEs的微生物代谢[18]，之后，Gordonia sp.､Rhodococcus sp.､Bacillus sp.等大批具有PAEs降解功能的菌株陆续被分离纯化[19-21].在PAEs-Cd复合污染土壤中，微生物面临多种PAEs和Cd共同胁迫，因此，将PAEs降解菌株固定至Cd钝化材料，制备固定化功能菌剂，有望实现PAEs降解和Cd钝化的协同修复[22].生物炭和蒙脱石都是具备良好Cd钝化和负载微生物功能的材料[23-25]，常用于PAEs和Cd污染土壤修复[26-27].将生物炭和蒙脱石复合，有望得到理想的微生物固定载体和Cd钝化材料，研制固定化菌剂，实现对PAEs-Cd复合污染土壤的协同修复.对此，国内外相关研究较少.
本研究提出以生物炭-巯基蒙脱石复合材料为固定化载体，构建具有耐Cd和PAEs降解功能的细菌菌群，采用吸附法制备固定化菌剂，研究固定化菌剂对土壤PAEs-Cd复合污染的协同修复效能，旨在为农田土壤PAEs-Cd复合污染控制与修复提供参考.
1　材料与方法
收起
1.1　试验材料
1.1.1　主要试剂
6种EPA优控塑化剂PAEs，包括邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)、邻苯二甲酸丁基苄基酯(BBP)、邻苯二甲酸二(2-乙基)己酯(DEHP)、邻苯二甲酸正辛酯(DnOP)均为分析纯，购于上海麦克林生化科技股份有限公司.正己烷、丙酮为分析纯，甲醇、乙腈为色谱纯，购于南京化学试剂股份有限公司.蒙脱石为分析纯，购于上海麦克林生化科技股份有限公司.3-巯丙基三甲氧基硅烷(3-MPTs)为分析纯，购于上海笛柏生物科技有限公司.无水CdCl2为分析纯，购于上海阿拉丁生化科技股份有限公司.
1.1.2　供试菌株
试验使用菌株为本课题组已有的PAEs降解菌：戈登氏菌属(Gordonia sp. 24107)、红球杆菌属(Rhodococcus sp. 2G)、芽孢杆菌属(Bacillus sp. W34).
1.1.3　培养基
试验所用培养基均为LB培养基和无机盐(MSM)培养基，配置方法为LB培养基：进口蛋白胨10.0g/L，进口酵母提取物5.0g/L，NaCl 10.0g/L.
MSM培养基：1.50g/L (NH4)2SO4,0.5g/L KH2PO4,1.91g/L K2HPO4·3H2O,0.5g/L NaCl, 0.20g/L MgSO4·7H2O.使用前均需使用高压灭菌锅121℃灭菌20min.
1.1.4　供试土壤
广西岩溶区采集的重金属-塑化剂复合污染土壤，其理化性质见表1.
1.2　复合菌群的制备
1.2.1　PAEs降解功能单菌的耐Cd驯化
将实验室前期已富集、筛选、分离、纯化获得的具有PAEs降解功能的单一功能菌株戈登氏菌属(Gordonia sp.24107)、红球杆菌属(Rhodococcus sp. 2G)、芽孢杆菌属(Bacillus sp. W34)使用液体LB培养基，在30℃、150r/min恒温培养箱中活化24h，在0~30mg/L不同Cd2+浓度LB培养基中梯度诱导驯化培养，稀释涂布，得到具有耐Cd和PAEs降解功能的单菌.
1.2.2　复配菌群的构建
将3种功能单菌在LB培养基中，温度30℃、150r/min条件下活化24h，并使用无机盐培养基离心，弃上清液，重悬2次，使用紫外分光光度计调OD600至1.0.拮抗试验发现24107、2G、W34之间不存在拮抗反应，按体积比1:1:1复配，4℃冰箱冷藏.
1.2.3　驯化菌株与固定化菌剂PAEs降解功能验证
向灭菌的锥形瓶中加入PAEs混标，使6种PAEs浓度为5mg/L，加入19mLMSM液体培养基和1mL菌悬液，设置未加菌液的空白对照，每个处理3个重复，在30℃、150r/min条件下避光培养，5d后取样测定PAEs残留浓度.
1.3　固定化载体材料的制备
1.3.1　生物炭制备
称取适量玉米秸秆粉末于坩埚，置于气氛炉中，密封并通入氮气，形成无氧环境.持续通入氮气为保护气，升温至裂解温度600℃，恒温炭化2h.冷却后用0.1%的HCl溶液清洗，去除热解过程产生的焦油和灰分，用超纯水冲洗多次后烘干.
1.3.2　巯基改性蒙脱石的制备
称取适量钙基蒙脱石加入含有3-巯基丙基三甲氧基硅烷(3-MPTs)的乙醇-水溶液中充分混合，调整固液比为1g:20mL、25℃温度下持续搅拌反应6h，离心，烘干，制得巯基改性蒙脱石.
1.3.3　生物炭-巯基蒙脱石复合载体的制备
称取适量巯基改性蒙脱石，加入去离子水，持续搅拌1h，制得巯基蒙脱石悬浮液;按生物炭和蒙脱石质量比3:1加入玉米生物炭，持续搅拌2h，离心，烘干，121℃下灭菌30min，制得生物炭-巯基蒙脱石复合载体.
1.3.4　载体材料Cd吸附能力测定
取0.1000g载体材料加入20mL含0.1mol/L KNO3的100mg/L Cd2+溶液体系，调pH值为6.0，在25℃恒温培养箱振荡1h，静置20h，定时取样，离心30min，取上清液过0.45μm水系滤膜，使用ICP-OES测定Cd2+浓度.
1.4　固定化菌剂的制备及条件优化
称取按1.3制备的生物炭-改性蒙脱石复合载体5g，将其加入100mL按1.2复配的菌群悬液(调OD600为1.0)，均匀混合.混合体系在恒温30℃、150r/min条件下避光振荡培养1d，并在无菌条件下过滤，冲洗，25℃烘干，制得5g的固定化功能菌剂.
1.4.1　固定时间对固定化菌剂降解PAEs的影响
按1.4中流程，分别培养1,2,3d，制备固定化菌剂，投加至1.2.3中体系，验证PAEs降解能力，确定最佳固定时间.
1.4.2　菌料比对固定化菌剂降解PAEs的影响
在最佳固定时间条件下，每1g固定化载体分别添加10,20和30mL复配菌群悬液，制得固定化载体.同上，验证PAEs降解能力，确定最佳菌料比.
1.4.3　固定温度对固定化菌剂降解PAEs的影响
在最佳固定时间和菌液用量的固定条件下，分别设置恒温培养箱在25,30,35℃条件下制备固定化菌剂.同上，验证PAEs降解能力，确定最佳固定温度.
1.5　固定化菌剂降解PAEs的动力学研究
向灭菌的锥形瓶中加入PAEs混标，各PAEs浓度为5mg/L，加入19mLMSM液体培养基和1g固定化菌剂，设置未加菌液的空白对照，每个处理3个重复，在30℃,150r/min条件下避光培养，连续7d每24h取样测定PAEs残留浓度，绘制PAEs降解曲线，利用Origin 2021进行拟合.
1.6　固定化菌剂对PAEs-Cd复合污染土壤修复作用验证
以1%的投加量施用菌剂至PAEs-Cd复合污染土壤中，保持土壤湿度为最大持水量的60%，在25℃恒温下避光培养50d，定期搅拌均匀，以未添加菌剂的处理组为对照，测定PAEs含量与Cd赋存形态.
1.7　分析方法
1.7.1　PAEs的检测方法
采用LC-20AT高效液相色谱仪.样品检测时长40min，进样量20μL，流动相为乙腈-水，初始流速1.0mL/min，以梯度洗脱分离6种PAEs，色谱柱为ϕ4.6mm×250mm Inertsil ODS-P液相色谱柱，柱温40℃，使用紫外检测器､在205和225nm双波长检测.6种PAEs加标回收率范围在80.03%~102.05%，相对标准偏差为0.52%~4.36%，本实验方法符合要求.
式中：Ck为无菌对照组处理PAEs的残留浓度，mg/L;CB为菌群或菌剂培养液中PAEs的残留浓度，mg/L.
1.7.2　Cd的检测方法
采用BCR连续提取法测定土壤Cd赋存形态.
F1可交换态和碳酸盐结合态：准确称取1.00g过0.15mm尼龙筛的风干土壤样品，置于50mL离心管内，加入8mL浓度为1mol/L的MgCl2溶液，用稀HCl调pH值至7.0，常温振荡1h，然后4000r/min离心10min，上清液过滤置于50mL容量瓶，用去离子水清洗滤纸3次后定容.
F2铁锰氧化物结合态：向上一步残余物中加入8mL 1mol/L的NaOAc溶液，并用HOAc调节pH值至5.0，常温下220r/min连续振荡5h，然后在4000r/min下离心10min，上清液过滤置于50mL容量瓶中，去离子水清洗滤纸3次后定容.
F3有机物结合态：向上一步残余物加入3mL 0.02mol/L的HNO3溶液，再加入5mL 30%的H2O2(稀HNO3调pH值为2.0)，于85℃的水浴加热并搅拌反应2h，再加入3mL 30%的H2O2,85℃的水浴加热并间歇搅拌反应3h，冷却后再加入5mL含3.2mol/L NH4OAc的20%的HNO3溶液，并加入约15mL去离子水稀释至20mL，常温连续振荡30min,4000r/min下离心10min，上清液过滤置于50mL容量瓶，去离子水清洗滤纸3次后定容.
F4残渣态：采用差减法，全Cd减去前3种形态之和即为残渣态含量.
四步提取样品采用ICP-OES分析浓度.
1.8　数据处理
使用Excel 2021处理数据，选用Tukey's HSD法进行不同处理的多重比较，采用SPSS 23.0中ANOVA软件进行方差分析，显著性水平为0.05.使用Origin 2021绘制图表.
2　结果与讨论
收起
2.1　复配菌群对PAEs的降解作用
2.1.1　驯化菌株对PAEs降解效能
由图1所示，菌株经过Cd2+梯度驯化后，可在Cd2+胁迫下保持对不同分子量PAEs的特异性降解功能.菌株24107(Gordonia sp.)ΣPAEs降解率可达87.91%，对低分子量PAEs(LMW-PAEs)及中分子量PAEs(MMW-PAEs)的降解率超过94%.菌株2G(Rhodococcus sp.)和W34(Bacillus sp.)对MMW-PAEs的降解率超过90%.
2.1.2　复配菌群PAEs降解效能
通过3种菌株的复配，根据图2所示，菌群对于高分子量PAEs(HMW-PAEs)的降解率由单菌的34.6%提升至87.9%,ΣPAEs降解率可达92.7%.菌群复配后对供试PAEs降解谱更宽，降解效能也得到提升，各降解菌株可通过协同代谢形成互养关系.李静等[28]从活性污泥土著菌群驯化得菌群SD-1，对DMP、DEP和DBP 3d降解率为68.7%、68.5%和80.0%.本研究得到的菌群降解谱更宽，覆盖6种EPA优控PAEs，并且降解效能更强，有望用于实际土壤PAEs污染的修复.
2.2　固定化载体材料性状
2.2.1　生物炭-巯基蒙脱石复合载体性状
由图3所示，玉米生物炭与巯基蒙脱石复合后，表面孔隙变大，出现稳定的多孔网状结构，大大提升了材料的孔径和比表面积.巯基蒙脱石粒径较小，附着在生物炭的表面或孔隙结构中，减少了杂质的附着.玉米生物炭和巯基蒙脱石复合材料增加了大量吸附点位，Cd固定能力得到增强，且适于微生物负载，是制备PAEs-Cd协同修复功能菌剂的理想载体材料.
图4的FT-IR图谱表明，生物炭与巯基蒙脱石复合后改善了表面官能团性状.复合材料表面红外吸收峰的位置主要出现在791,1080,1613,2980,3422cm-1等处.3600~3100cm-1处为O-H键的伸缩振动，表明生物炭和巯基蒙脱石复合后疏水性变差.2980cm-1处的吸收峰为C-H键的吸收振动，1613cm-1处的吸收峰为C=O键的吸收振动，表明复合材料石墨化程度降低.1080cm-1处的吸收峰为Si-O-Si的伸缩振动，是巯基改性蒙脱石的特征吸收峰.
2.2.2　复合载体Cd吸附效能
图5为复合载体的Cd饱和吸附曲线，玉米生物炭与巯基蒙脱石复合后，Cd2+吸附性得到显著提升，菌剂对Cd饱和吸附量达15.2mg/kg，较生物炭Cd吸附量提升了1.11倍.复合材料加入Cd溶液中，各个时期Cd吸附量均高于生物炭，且8~10h吸附达到平衡，12h后略微降低，吸附法结合并不稳定，可能出现解吸现象.
2.3　固定化功能菌剂对PAEs的降解作用
2.3.1　固定化菌剂结构特征
从图6观察到生物炭-巯基蒙脱石复合材料的表面和孔隙结构中附着大量的功能菌株，且菌株以团聚形式或分散状态附着在复合材料中，形状较为饱满，表明固定化菌剂对功能微生物具有良好的负载效能.
2.3.2　固定化菌剂制备条件优化
根据图7，其他条件一致，固定时间为1d制备的菌剂PAEs降解率显著高于其他时间.结合菌株的生长特性，3种菌株在24h内可能处于对数生长期，代谢活动最强，污染物降解效能最高[29].若固定化时间延长，菌株生长进入衰亡期，菌群降解能力不佳.另外，若固定时间太短，复合载体对于菌株的吸附固定不充分，将导致菌剂PAEs降解效能较差.
控制其他条件一致，菌料比为1:20制备的固定化菌剂PAEs降解率显著高于其他比例.在细菌菌群负载到载体材料过程中，若菌群数量太少，细菌进入载体吸附点位的概率降低，污染物降解能力不足;若细菌数量过多，可能导致对底物的竞争[30]，不利于细菌生长定殖，使污染物降解效果不佳.
其他条件一致的情况下，30℃制备的固定化菌剂PAEs降解能力显著优于其他温度.功能细菌降解PAEs多为酶促反应，与温度密切关联[31].过高的温度使细菌体内相关酶活性下降，降解能力减弱.反之温度过低会降低细胞膜的流动性，不利于PAEs作为底物进入生物循环，污染物降解能力减弱.
固定化菌剂制备的最佳负载条件为菌料比1g:20mL,30℃恒温振荡培养1d，该条件下制备的固定化菌剂对6种PAEs降解效果最佳.由图8可见，最优条件下制备的固定化菌剂，ΣPAEs降解率可达95.4%，较菌群降解能力略有提升，高分子量PAEs的降解率提升至85%以上，具备高效性和广谱性.
2.3.3　固定化菌剂对PAEs的降解动力学
在纯培养体系下，菌剂对6种PAEs均具备良好的降解能力，由图9(a)降解曲线所示，第1d时，菌剂对LMW-PAEs和MMW-PAEs的降解率均高于80%，对HMW-PAE降解率为32.7%和35.3%.培养1~3d，菌剂对HMW-PAEs降解率逐渐提高，对LMW-PAEs的降解趋于稳定，均在85%以上，降解能力优良.培养4~7d，随时间延长，菌剂降解趋于平稳.以上结果表明，固定化菌剂降解PAEs具有广谱性，对LMW-PAEs的降解具有高效性.在培养初期，HMW-PAEs降解功能菌株可能经历适应阶段合成相关的酶，或调整代谢途径，随时间延长，对其降解率逐渐提高.利用一级动力学方程对PAEs降解曲线进行拟合，方程如下：
式中：C0为初始底物浓度(mg/L);C为底物浓度(mg/L);t为降解时间(d);K为一级反应速率常数(d-1);A为常数.
如图9(b)和表2所示，菌剂对6种PAEs的降解过程均符合伪一级反应动力学，PAEs残留浓度的自然对数与降解时间线性相关.LMW-PAEs半衰期较短，仅2.2d左右；HMW-PAEs和MMW-PAEs半衰期较长，与PAEs性质和微生物代谢途径相关.
2.4　固定化菌剂对复合污染土壤的修复效能
2.4.1　固定化菌剂对复合污染土壤中PAEs的消减作用
根据图10(a)，按1%投加量施用固定化菌剂至复合污染土壤，ΣPAEs含量由2.56mg/kg降至1.17mg/kg，与同时期CK相比ΣPAEs消减率为54.14%.目前有关PAEs降解微生物的研究局限于溶液培养体系，使用固定化菌剂处理PAEs污染土壤的研究较少.褚美函[32]利用生物炭制备固定化菌剂，以5%投加量，处理东北矿区重金属Cd-多环芳烃(PAHs)复合污染土壤60d后菌剂对萘的降解率为52.39%.参考这一表现，本研究制备的固定化菌剂具有理想的PAEs消减效能.
2.4.2　固定化菌剂对复合污染土壤中Cd的钝化作用
根据图10(b)，施用1%固定化菌剂后，复合污染土壤中可交换态Cd含量较CK降低了37.06%，碳酸盐结合态､铁锰氧化物结合态和残渣态Cd分别增加了19.26%、13.00%和12.55%.在土壤中，可交换态Cd活性更强且易于被植物吸收，碳酸盐结合态在偏碱性土壤中相对稳定，铁锰氧化态含量在氧化环境下也相对稳定.Zhao等[33]按1%投加量施用改性煤矸石MGE至重金属Cd污染土壤，植物根部有效态Cd含量降低了20.4%.Bashir等[34]将水稻生物炭和沸石及磷酸盐结合处理Cd污染土壤，投加量为3%时土壤有效Cd下降了18.18%~37.66%.相对比下，本研究制备的固定化菌剂具有良好的Cd钝化效能.固定化菌剂的施用可能通过提高土壤pH值，复合材料表面的阳离子交换作用和巯基官能团的络合作用等途径，使土壤中Cd2+由活性较强的可交换态向稳定的碳酸盐结合态､铁锰氧化物结合态和残渣态转化，大大降低了Cd的生物有效性.
3　结论
收起
3.1　本研究构建了具备耐Cd和PAEs降解功能的菌群，6种PAEs总降解率达92.7%，对高分子量PAEs降解率提升至85%以上，具备高效性和广谱性.
3.2　制备了巯基改性蒙脱石和生物炭复合载体，对Cd饱和吸附量为15.2mg/kg，较生物炭提升了1.11倍.
3.3　制备了固定化菌剂，优化了制备条件.菌剂最优制备条件为温度30℃，固定时间1d，菌料比1:20(V/M)，最佳条件下制备的固定化菌剂ΣPAEs降解率达95.4%.
3.4　固定化菌剂对土壤PAEs和Cd复合污染具有良好的协同修复作用.以1%投加量施用菌剂，50d后复合污染土壤中ΣPAEs消减率为54.14%，可交换态Cd减少了37.06%.
基金
收起
  • 国家自然科学基金资助项目(U22A20590; 42430703)
  • 国家重点研发计划项目(2023YFE0110800)
  • 江苏省农业科技自主创新资金(CX（24）1002)
文献
收起
参考文献 引证文献
排序方式：
[1]
Shi J，Yu X，Zhang M，et al. Potential risks of copper，zinc，and cadmium pollution due to pig manure application in a soil-rice system under intensive farming: a case study of Nanhu，China [J]. Journal of Environmental Quality，2011，40(6):1695-1704.
[2]
Wang J，Luo Y，Teng Y，et al. Soil contamination by phthalate esters in Chinese intensive vegetable production systems with different modes of use of plastic film [J]. Environmental Pollution，2013，180:265-273.
[3]
Olaniran A O，Balgobind A，Pillay B. Bioavailability of heavy metals in soil: impact on microbial biodegradation of organic compounds and possible improvement strategies [J]. International Journal of Molecular Sciences，2013，14(5):10197-10228.
[4]
Rahman Z. An overview on heavy metal resistant microorganisms for simultaneous treatment of multiple chemical pollutants at co- contaminated sites，and their multipurpose application [J]. Journal of Hazardous Materials，2020，396:122682.
[5]
Zhao S，Xu W，Zong S，et al. The pollution characteristics and full-scale risk assessment of coking plant soil co-contaminated with polycyclic aromatic hydrocarbons and heavy metals [J]. Environmental Engineering Science，2021，38(9):867-876.
[6]
Aitio A，Tritscher A. Effects on health of cadmium-WHO approaches and conclusions [J]. Biometals，2004，17:491.
[7]
Genchi G，Sinicropi M S，Lauria G，et al. The effects of cadmium toxicity [J]. International Journal of Environmental Research and Public Health，2020，17(11):3782.
[8]
Wilson S C，Meharg A A. Investigation of organic xenobiotic transfers，partitioning and processing in air-soil-plant systems using a microcosm apparatus. Part II: comparing the fate of chlorobenzenes in grass planted soil [J]. Chemosphere，2003，53:583-591.
[9]
Antoniadis V，Levizou E，Shaheen S M，et al. Trace elements in the soil-plant interface: Phytoavailability，translocation，and phytoremediation-A review [J]. Earth-Science Reviews，2017，171:621-645.
[10]
Chen H，Teng Y，Lu S，et al. Contamination features and health risk of soil heavy metals in China [J]. Science of The Total Environment，2015，512:143-153.
[11]
Yang Q，Yang Z，Zhang Q，et al. Ecological risk assessment of Cd and other heavy metals in soil-rice system in the karst areas with high geochemical background of Guangxi，China [J]. Science China Earth Sciences，2021，64(7):1126-1139.
[12]
骆永明，滕应.中国土壤污染与修复科技研究进展和展望[J]. 土壤学报，2020，57(5):1137-1142.
Luo Y M，Teng Y. Progress and Prospects in Soil Pollution and Remediation Research in China [J]. Acta Pedologica Sinica，2020，57(5):1137-1142.
[13]
Deary M E，Ekumankama C C，Cummings S P. Effect of lead，cadmium，and mercury co-contaminants on biodegradation in PAH-polluted soils [J]. Land Degradation and Development，2018，29(6):1583-1594.
[14]
Otitoloju A A. Evaluation of the joint-action toxicity of binary mixtures of heavy metals against the mangrove periwinkle Tympanotonus fuscatus var radula (L.) [J]. Ecotoxicology and Environmental Safety，2002，53(3):404-415.
[15]
Zheng Z，Zhou P，Wu Z. New advances in research of combined pollution [J]. Journal of Applied Ecology，2001，12(3):469-473.
[16]
Gao Y Z，Xiong W，Ling W T，et al. Sorption of phenanthrene by soils contaminated with heavy metals [J]. Chemosphere，2006，65(8):1355-1361.
[17]
Wang X，Wu H，Dai C，et al. Microbial interactions enhanced environmental fitness and expanded ecological niches under dibutyl phthalate and cadmium co-contamination [J]. Environmental Pollution，2022，306:119362.
[18]
Engelhardt G，Wallnofer P R，Hutzinger O，et al. The microbial metabolism of di-n-butyl phthalate and related dialkyl phthalates [J]. Bulletin of Environmental Contamination and Toxicology，1975，13:342-347.
[19]
Chatterjee S，Dutta T K. Metabolism of butyl benzyl phthalate by Gordonia sp. strain MTCC 4818 [J]. Biochemical and Biophysical Research Communications，2003，309(1):36-43.
[20]
Wang J L，Ye Y C，Wu W Z. Comparison of di-n-methyl phthalate biodegradation by free and immobilized microbial cells [J]. Biomedical and Environmental Sciences，2003，16(2):126-132.
[21]
Jin D C，Liang R X，Dai Q Y，et al. Biodegradation of di-n-butyl phthalate by Rhodococcus sp. JDC-11and molecular detection of 3，4-phthalate dioxygenase gene [J]. Journal of Microbiology and Biotechnology，2010，20(10):1440-1445.
[22]
Priyanka J V，Rajalakshmi S，Kumar P S，et al. Bioremediation of soil contaminated with toxic mixed reactive azo dyes by co-cultured cells of Enterobacter cloacae and Bacillus subtilis [J]. Environmental Research，2022，204:112-136.
[23]
Hagemann N，Joseph S，Schmidt H P，et al. Organic coating on biochar explains its nutrient retention and stimulation of soil fertility [J]. Nature communications，2017，8(1):1089.
[24]
Guo D，Xia Q，Zeng Q，et al. Antibacterial mechanisms of reduced iron-containing smectite–illite clay minerals [J]. Environmental Science and Technology，2021，55(22):15256-15265.
[25]
Van G N，Christl I，Kretzschmar R，et al. The effect of aeration on Mn(II) sorbed to clay minerals and its impact on Cd retention [J]. Environmental Science and Technology，2021，55(3):1650-1658.
[26]
Abbas T，Rizwan M，Ali S，et al. Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in a soil with aged contamination [J]. Ecotoxicology and Environmental Safety，2017，140:37-47.
[27]
Guimarães A M，Ciminelli V S，Vasconcelos W L，et al. Smectite organofunctionalized with thiol groups for adsorption of heavy metal ions [J]. Applied Clay Science，2009，42(3/4):410-414.
[28]
李静.邻苯二甲酸酯降解菌的降解特性与降解机制的初步研究[D]. 重庆:西南大学，2018.
Li J. Preliminary study on the degrading charicteristics and mechanisms of phthalic acid esters-degrading bacteria [D]. Chongqing: Southwest University，2018
[29]
Yates G T，Smotzer T. On the lag phase and initial decline of microbial growth curves [J]. Journal of Theoretical Biology，2007，244(3):511-517.
[30]
陆雯逸，王泽铭，左翔之，等.固定化菌剂对邻苯二甲酸酯的降解效能及途径[J]. 中国环境科学，2024，44(3):1584-1591.
Lu W Y，Wang Z M，Zuo X Z，et al. The degradation efficiency and pathways of phthalic acid esters by immobilized bacterial agent [J]. China Environmental Science，2024，44(3):1584-1591.
[31]
张小红，陶红，王亚娟，等.固定化微球降解土壤中PAEs效果及影响因素[J]. 生物技术通报，2021，37(1):223.
Zhang X H，Tao H，Wang Y J，et al. Effect and influencing factors of immobilized microspheres on degradation of phthalate esters in soil [J]. Biotechnology Bulletin，2021，37(1):223-233.
[32]
褚美函.固定化菌剂对复合污染土壤的修复效果及微生物群落结构的影响[D]. 沈阳:沈阳大学，2024.
Chu M H. The remediation effect of immobilized microbial agents on co-contaminated soils and their impact on microbial community structure [D]. Shenyang: Shenyang University，2024.
[33]
Zhao H，Li P，He X，et al. Remediation of cadmium contaminated soil by modified gangue material: characterization，performance and mechanisms [J]. Chemosphere，2022，290:133347.
[34]
Bashir S，Zhu J，Fu Q，et al. Cadmium mobility，uptake and anti-oxidative response of water spinach (Ipomoea aquatic) under rice straw biochar，zeolite and rock phosphate as amendments [J]. Chemosphere，2018，194:579-587.
2025年第45卷第6期
PDF下载
53
24
引用本文
BibTeX
文章信息
  • 接收时间：2024-12-15
  • 首发时间：2026-02-27
  • 出版时间：2025-06-20
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2024-12-15
基金
国家自然科学基金资助项目(U22A20590; 42430703)
国家重点研发计划项目(2023YFE0110800)
江苏省农业科技自主创新资金(CX（24）1002)
作者信息
    南京农业大学，土壤有机污染控制与修复研究所，江苏 南京 211800

通讯作者:

* 责任作者，教授，
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/zghjkx/CN/1234106390516659030
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持