首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1307007628861796566, tenantId=1146029695717560320, journalId=1306274286668972124, issueId=1307007396681900041, articleNumber=null, orderNo=null, doi=10.11843/j.issn.0366-6964.2026.01.031, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1744732800000, receivedDateStr=2025-04-16, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1789544501747, onlineDateStr=2026-09-16, pubDate=1769097600000, pubDateStr=2026-01-23, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789544501747, onlineIssueDateStr=2026-09-16, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789544501747, creator=13701087609, updateTime=1789544501747, updator=13701087609, issue=Issue{id=1307007396681900041, tenantId=1146029695717560320, journalId=1306274286668972124, year='2026', volume='57', issue='1', pageStart='1', pageEnd='568', issueExtLink='null', onlineDate='null', pubDate='1769097600000', pubDateStr='2026-01-23', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789544446392, creator='13701087609', updateTime=1789609890784, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1307281890638057732, tenantId=1146029695717560320, journalId=1306274286668972124, issueId=1307007396681900041, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1307281890738721029, tenantId=1146029695717560320, journalId=1306274286668972124, issueId=1307007396681900041, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=360, endPage=368, ext={EN=ArticleExt(id=1307007629298004183, articleId=1307007628861796566, tenantId=1146029695717560320, journalId=1306274286668972124, language=EN, title=Detection of Virulence Genes and Drug Resistance Analysis of 15 Serotypes of Riemerella anatipestifer, columnId=1307007432299930010, journalTitle=Acta Veterinaria et Zootechnica Sinica, columnName=PREVENTIVE VETERINARY MEDICINE, runingTitle=null, highlight=null, articleAbstract=

In order to understand the drug resistance and virulence gene distribution of Riemerella anatipestifer isolates in some areas of China， K-B method was used to determine the sensitivity of 15 serotypes of R. anatipestifer to 10 commonly used antibiotics， and PCR technology was used to detect 16 drug resistance genes and 16 virulence genes of different serotypes of R. anatipestifer isolates. The results showed that the resistance rates of amoxicillin and ampicillin were 93.33% and 80%， respectively. The detection results of drug-resistant genes showed that the detection rates of tet（X） gene of tetracycline， floR gene of amide alcohol and aph（3'）-Ⅱ gene of aminoglycoside were 93.33%， 80% and 73.33% respectively. The results of virulence gene detection showed that tctR， TbdR1， TR， CAMP and AS87-0405 had the highest detection rates， which were 86.67%， 86.67%， 80%， 73.33% and 73.33% respectively. These isolates are sensitive to tetracyclines， quinolones and amide alcohols， and have high resistance to β-lactams. At the same time， these isolates carry a variety of virulence genes， which may indicate that they have strong virulence. The above results provide some reference for the prevention and control of R. anatipestifer in some areas of China.

, authors=Chenyang LI1, Chuanmei ZHANG2, Yutao HUI2, Ying LÜ2, Min YANG1, authorsList=Chenyang LI, Chuanmei ZHANG, Yutao HUI, Ying LÜ, Min YANG, authorCompany=null, correspAuthors=Min YANG, authorNote=null, correspAuthorsNote=
YANG Min， E-mail：
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307007632460509405, articleId=1307007628861796566, tenantId=1146029695717560320, journalId=1306274286668972124, language=CN, title=15种血清型的鸭疫里默氏杆菌毒力基因检测和耐药性分析, columnId=1307007432459313564, journalTitle=畜牧兽医学报, columnName=预防兽医, runingTitle=null, highlight=null, articleAbstract=

旨在了解我国部分地区鸭疫里默氏杆菌分离株的耐药性及毒力基因分布情况，采用K-B法测定15种血清型鸭疫里默氏杆菌对10种临床常用抗菌药物的敏感性，同时采用PCR技术对不同血清型RA分离株进行了16种耐药基因和16种毒力基因的检测。结果表明，菌株对阿莫西林的耐药率为93.33%，对氨苄西林的耐药率为80%；耐药基因检测结果显示，四环素类的tet（X）基因、酰胺醇类的floR基因、氨基糖苷类的aph（3'）-Ⅱ基因的检出率分别为93.33%、80%、73.33%。毒力基因检测结果显示，tctR、TbdR1、TR、CAMP和AS87-0405毒力基因检出率最高，分别为86.67%、86.67%、80%、73.33%、73.33%。这些分离株对四环素类、喹诺酮类、酰胺醇类药物较敏感，对β-内酰胺类药物具有较高的耐药性；同时这些分离株携带多种毒力基因，可能预示着其具有较强毒力。以上结果为我国部分地区鸭疫里默氏杆菌病防控提供了一定的参考依据。

, authors=李晨阳1, 张传美2, 惠宇韬2, 吕莹2, 杨敏1, authorsList=李晨阳, 张传美, 惠宇韬, 吕莹, 杨敏, authorCompany=null, correspAuthors=杨敏, authorNote=

李晨阳，博士，讲师，主要从事动物病原菌的耐药性研究，E-mail：

, correspAuthorsNote=
杨敏，主要从事药物化学研究，E-mail：
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=X4K+mrJ+nAV8gm4Hcw+gzw==, magXml=rAAUKxETD0dSyKTh7L76KA==, pdfUrl=null, pdf=5I5g2jEN5JCLdD4cfdjscw==, pdfFileSize=545202, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=qQMMP46bqmh0PaUqIDUGbA==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=Eus2wNcvxp10WPdDpaG0/g==, mapNumber=null, fund=null)}, authors=[Author(id=1307286561163338639, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=1003354610@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561234641809, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561163338639, language=EN, stringName=Chenyang LI, firstName=Chenyang, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286561377248146, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561163338639, language=CN, stringName=李晨阳, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.甘肃农业大学动物医学院，兰州 730070, bio={"content":"

李晨阳，博士，讲师，主要从事动物病原菌的耐药性研究，E-mail：

"}, bioImg=null, bioContent=

李晨阳，博士，讲师，主要从事动物病原菌的耐药性研究，E-mail：

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286560957817736, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=1., ext=[AuthorCompanyExt(id=1307286560991372169, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China), AuthorCompanyExt(id=1307286561003955082, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.甘肃农业大学动物医学院，兰州 730070)])]), Author(id=1307286561444357012, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561524048790, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561444357012, language=EN, stringName=Chuanmei ZHANG, firstName=Chuanmei, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286561591157655, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561444357012, language=CN, stringName=张传美, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛农业大学动物医学院，青岛 266109, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286561083646859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=2., ext=[AuthorCompanyExt(id=1307286561092035468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China), AuthorCompanyExt(id=1307286561100424077, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛农业大学动物医学院，青岛 266109)])]), Author(id=1307286561662460825, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561737958299, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561662460825, language=EN, stringName=Yutao HUI, firstName=Yutao, middleName=null, lastName=HUI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286561809261468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561662460825, language=CN, stringName=惠宇韬, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛农业大学动物医学院，青岛 266109, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286561083646859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=2., ext=[AuthorCompanyExt(id=1307286561092035468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China), AuthorCompanyExt(id=1307286561100424077, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛农业大学动物医学院，青岛 266109)])]), Author(id=1307286561872176030, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561943479200, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561872176030, language=EN, stringName=Ying LÜ, firstName=Ying, middleName=null, lastName=LÜ, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286562002199457, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561872176030, language=CN, stringName=吕莹, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛农业大学动物医学院，青岛 266109, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286561083646859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=2., ext=[AuthorCompanyExt(id=1307286561092035468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China), AuthorCompanyExt(id=1307286561100424077, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛农业大学动物医学院，青岛 266109)])]), Author(id=1307286562094474147, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=yummy12@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1307286562207720357, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286562094474147, language=EN, stringName=Min YANG, firstName=Min, middleName=null, lastName=YANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286562279023526, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286562094474147, language=CN, stringName=杨敏, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.甘肃农业大学动物医学院，兰州 730070, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286560957817736, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=1., ext=[AuthorCompanyExt(id=1307286560991372169, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China), AuthorCompanyExt(id=1307286561003955082, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.甘肃农业大学动物医学院，兰州 730070)])])], keywords=[Keyword(id=1307286562438407079, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, orderNo=1, keyword=Riemerella anatipestifer), Keyword(id=1307286562505515944, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, orderNo=2, keyword=virulence gene), Keyword(id=1307286564158071721, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, orderNo=3, keyword=drug resistance gene), Keyword(id=1307286564296483754, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, orderNo=4, keyword=drug-fast), Keyword(id=1307286564380369835, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, orderNo=1, keyword=鸭疫里默氏杆菌), Keyword(id=1307286564464255916, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, orderNo=2, keyword=毒力基因), Keyword(id=1307286564531364781, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, orderNo=3, keyword=耐药基因), Keyword(id=1307286564598473646, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, orderNo=4, keyword=耐药性)], refs=[Reference(id=1307286566582379453, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=李富祥，赵文华，宋建领，等.鸡源鸭疫里默杆菌的分离鉴定及ompA基因遗传进化分析［J］.中国兽医学报，2021，41（2）：253-258., articleTitle=null, refAbstract=null), Reference(id=1307286566653682622, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=LI F X，ZHAO W H，SONG J L，et al.Isolation and identification of Riemerella anatipestifer from Chickens and genetic evolutionary analysis of its ompA gene.1［J］.Chinese Journal of Veterinary Medicine，2021，41（2）：253-258.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286566724985791, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=CHEN S，ZENG X，ZHENG X，et al.Identification of a highly pathogenic Riemerella anatipestifer strain causing duck spleen marble-like necrosis disease in China［J］.Microb Pathog，2024，196：106912., articleTitle=null, refAbstract=null), Reference(id=1307286566792094656, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=欧芳杏，张 霞，陈佳琪，等.2021—2023年贵州省鸭疫里默氏杆菌病流行病学调查［J］.中国畜禽种业，2024，20（6）：110-119., articleTitle=null, refAbstract=null), Reference(id=1307286566863397825, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=OU F X，ZHANG X，CHEN J Q，et al.Epidemiological survey of Riemerella anatipestifer disease in guizhou province from 2021 to 2023［J］.Chinese Journal of Livestock and Poultry Breeding，2024，20（6）：110-119.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286566930506690, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=郭玉璞，陈德威，范国雄，等.北京鸭小鸭传染性浆膜炎的调查研究［J］.畜牧兽医学报，1982，2：35- 41., articleTitle=null, refAbstract=null), Reference(id=1307286566993421251, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=GUO Y P，CHEN D W，FAN G X，et al.Investigation and study on infectious serositis in ducklings of Peking duck［J］.Acta Veterinaria et Zootechnica Sinica，1982，2：35-41.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286568666948548, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=YANG Z，YANG X，WANG M，et al.Genome-wide association study reveals serovar-associated genetic loci in Riemerella anatipestifer［J］.BMC Genomics，2024，25（1）：57-57., articleTitle=null, refAbstract=null), Reference(id=1307286568746640325, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=RYLL M，HINZ K H.Exclusion of strain 670/89 as type strain for serovar 20 of Riemerella anatipestifer［J］.Berl Munch Tierarztl Wochenschr，2000，113（2）：65-66., articleTitle=null, refAbstract=null), Reference(id=1307286568843109318, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=9, authorNames=null, journalName=null, refType=null, unstructuredReference=PATHANASOPHON P，PHUEKTES P，TANTICHAROENYOS T，et al.A potential new serotype of Riemerella anatipestifer isolated from ducks in Thailand［J］.Avian Pathol，2002，31（3）：267-270., articleTitle=null, refAbstract=null), Reference(id=1307286568922801095, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=10, authorNames=null, journalName=null, refType=null, unstructuredReference=PATHANASOPHON P，SAWADA T，TANTICHAROENYOS T.New serotypes of Riemerella anatipestifer isolated from ducks in Thailand［J］.Avian Pathol，1995，24（1）：195-199., articleTitle=null, refAbstract=null), Reference(id=1307286568994104264, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=11, authorNames=null, journalName=null, refType=null, unstructuredReference=陈国权，吴征卓，姚碧琼，等．鸭疫里默氏杆菌贵州流行株的分离鉴定及耐药性分析［J］.中国兽医科学，2020，50 （6） ：731-739．, articleTitle=null, refAbstract=null), Reference(id=1307286569065407433, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=12, authorNames=null, journalName=null, refType=null, unstructuredReference=CHEN G Q，WU Z Z，YAO B Q，et al.Isolation，identification and drug resistance analysis of Riemerella anatipestifer epidemic strains in Guizhou［J］.Chinese Veterinary Science，2020，50（6）：731-739.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286569145099210, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=13, authorNames=null, journalName=null, refType=null, unstructuredReference=程安春，汪铭书，陈孝跃，等.我国鸭疫里默氏杆菌血清型调查及新血清型的发现和病原特性［J］.中国兽医学报，2003，4：320-323., articleTitle=null, refAbstract=null), Reference(id=1307286569413534667, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=14, authorNames=null, journalName=null, refType=null, unstructuredReference=CHENG A C，WANG M S，CHEN X Y，et al.Serotype survey of Riemerella anatipestifer in China，discovery of new serotypes and pathogen characteristics［J］.Chinese Journal of Veterinary Medicine，2003，4：320-323.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286569493226444, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=15, authorNames=null, journalName=null, refType=null, unstructuredReference=祝希辉，李艳兰，王志伟，等.1株鸭疫里默氏杆菌的全基因组测序及耐药性与遗传进化分析［J］.中国畜牧兽医，2023，50（3）：1140-1149., articleTitle=null, refAbstract=null), Reference(id=1307286569556141005, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=16, authorNames=null, journalName=null, refType=null, unstructuredReference=ZHU X H，LI Y L，WANG Z W，et al.Whole-genome sequencing of one strain of Riemerella anatipestifer and analysis of its drug resistance and genetic evolution［J］.China Animal Husbandry & Veterinary Medicine，2023，50（3）：1140-1149.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286569644221390, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=17, authorNames=null, journalName=null, refType=null, unstructuredReference=张旭财，李燕红，陆毅兴，等.广东肇庆地区鸭疫里氏杆菌耐药性和毒力基因分析及ERIC-PCR分型研究［J］.中国动物传染病学报，2022，30（4）：39-47., articleTitle=null, refAbstract=null), Reference(id=1307286569719718863, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=18, authorNames=null, journalName=null, refType=null, unstructuredReference=ZHANG X C，LI Y H，LU Y X，et al.Analysis of drug resistance，virulence genes and ERIC-PCR typing of Riemerella anatipestifer in Zhaoqing area，Guangdong province［J］.Chinese Journal of Animal Infectious Diseases，2022，30（4）：39-47.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286569791022032, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[13], rfOrder=19, authorNames=null, journalName=null, refType=null, unstructuredReference=张 燕.鸭疫里默氏杆菌安徽分离株耐药性分析与致病性研究［D］.扬州：扬州大学，2022., articleTitle=null, refAbstract=null), Reference(id=1307286569853936593, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[13], rfOrder=20, authorNames=null, journalName=null, refType=null, unstructuredReference=ZHANG Y.Analysis of drug resistance and study on pathogenicity of Riemerella anatipestifer isolates from Anhui［D］.Yangzhou：Yangzhou University，2022.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286569916851154, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=21, authorNames=null, journalName=null, refType=null, unstructuredReference=王小兰.鸭疫里默氏杆菌烟酰胺酶基因的鉴定及功能研究［D］.北京：中国农业科学院，2018., articleTitle=null, refAbstract=null), Reference(id=1307286569988154323, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=22, authorNames=null, journalName=null, refType=null, unstructuredReference=WANG X L.Identification and functional study of the nicotinamidase gene in Riemerella anatipestifer［D］.Beijing：Chinese Academy of Agricultural Sciences，2018.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286570080429012, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=23, authorNames=null, journalName=null, refType=null, unstructuredReference=MAGIORAKOS A P，SRINIVASAN A，CAREY R B，et al.Multidrug-resistant，extensively drug-resistant and pandrug-resistant bacteria：an international expert proposal for interim standard definitions for acquired resistance［J］.Clin Microbiol Infect，2012，18（3）：268-281., articleTitle=null, refAbstract=null), Reference(id=1307286570151732181, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[16], rfOrder=24, authorNames=null, journalName=null, refType=null, unstructuredReference=常陈星，刘美含，杨跃飞，等.鸭疫里默氏杆菌氨基糖苷类药物的耐药性分析［J］.黑龙江畜牧兽医，2020，13：101-103，108., articleTitle=null, refAbstract=null), Reference(id=1307286570218841046, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[16], rfOrder=25, authorNames=null, journalName=null, refType=null, unstructuredReference=CHANG C X，LIU M H，YANG Y F，et al.Analysis of drug resistance of Riemerella anatipestifer to aminoglycoside antibiotics［J］.Heilongjiang Animal Science and Veterinary Medicine，2020，（13）：101-103，108.（in Chinese）, articleTitle=null, refAbstract=null), Reference(id=1307286570290144215, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=26, authorNames=null, journalName=null, refType=null, unstructuredReference=ZHU D，LUO H，LIU M，et al.Various profiles of tet genes addition to tet（X） in Riemerella anatipestifer isolates from ducks in China［J］.Front Microbiol，2018，9：585., articleTitle=null, refAbstract=null), Reference(id=1307286570353058776, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[18], rfOrder=27, authorNames=null, journalName=null, refType=null, unstructuredReference=TAO M，WANG J，LI K，et al.Development of signature tagged mutagenesis in Riemerella anatipestifer to identify genes essential for survival and pathogenesis［J］.Vet Microbiol，2020，250：108857., articleTitle=null, refAbstract=null)], funds=null, companyList=[AuthorCompany(id=1307286560957817736, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=1., ext=[AuthorCompanyExt(id=1307286560991372169, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China), AuthorCompanyExt(id=1307286561003955082, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.甘肃农业大学动物医学院，兰州 730070)]), AuthorCompany(id=1307286561083646859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=2., ext=[AuthorCompanyExt(id=1307286561092035468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China), AuthorCompanyExt(id=1307286561100424077, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛农业大学动物医学院，青岛 266109)])], figs=[ArticleFig(id=1307286564833354671, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, label=Fig.1, caption=PCR identification of isolated strains

M represents DL2000 marker; 1-17 represent strains sample of different serotypes; - represents negative control

, figureFileSmall=B859KO+JypsaJbWWIxpk1A==, figureFileBig=yM/xxuWkGfILMVksoJWgew==, tableContent=null), ArticleFig(id=1307286564900463536, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, label=图1, caption=部分分离菌PCR鉴定结果

M. DL2000 DNA相对分子质量标准；1-17. 不同血清型菌株样品；-. 阴性对照

, figureFileSmall=B859KO+JypsaJbWWIxpk1A==, figureFileBig=yM/xxuWkGfILMVksoJWgew==, tableContent=null), ArticleFig(id=1307286565005321137, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, label=Table 1, caption=

Sequence information of drug resistance gene primers

, figureFileSmall=null, figureFileBig=null, tableContent=

耐药基因

Resistance gene

引物序列（5'→3'）

Primer sequences

产物大小/bp

Product length

退火温度/℃

Annealing temperature

aac（6′）-ⅡF：TTGCGATGCTCTATGAGTGGCTA48052
R：CTCGAATGCCTGGCGTGTTT
aph（3'）-ⅡaF：TGACTGGGCACAACAGACA71754
R：TCAAGAAGGCGATAGAAGGC
aph（3'）-ⅡF：TCTGAAACATGGCAAAGGTAG58254
R：AGCCGTTTCTGTAATGAAGGA
rmtCF：CGAAGAAGTAACAGCCAAAG71155
R：ATCCCAACATCTCTCCCACT
tet （A）F：GCGCCTTTCCTTTGGGTTCT83151
R：CCACCCGTTCCACGTTGTTA
tet （B）F：CATTAATAGGCGCATCGCTG93055
R：TGAAGGTCATCGATAGCAGG
tet （C）F：GCTGTAGGCATAGGCTTGGT88855
R：GCCGGAAGCGAGAAGAATCA
tet （X）F：ATGACAATGCGAATAGATACAGAC1 16753
R：CAATTGCTGAAACGTAAAGTC
blaSHVF：CACTCAAGGATGTATTGTG88555
R：TTAGCGTTGCCAGTGCTCG
blaDHAF：AACTTTCACAGGTGTGCTGT38756
R：CCGTACGCATACTGGCTTTC
qnrAF：ATTTCTCACGCCAGGATTTG51658
R：GATCGGCAAAGGTTAGGTCA
qnrBF：GATCGTGAAAGCCAGAAAGG46958
R：ACGATGCCTGGTAGTTGTCC
qnrSF：ACGACATTCGTCAACTGCAA41753
R：TAAATTGGCACCCTGTAGGC
Sul1F：GTGACGGTGTTCGGCATTCT77968
R：CCGAGAAGGTGATTGCGCT
Sul2F：CCTGTTTCGTCCGACACAGA43551
R：GAAGCGCAGCCGCAATTCAT
FloRF：CTGAGGGTGTCGTCATCTAC67358
R：GCTCCGACAATGCTGACTAT
), ArticleFig(id=1307286565168898994, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, label=表1, caption=

耐药基因引物序列信息

, figureFileSmall=null, figureFileBig=null, tableContent=

耐药基因

Resistance gene

引物序列（5'→3'）

Primer sequences

产物大小/bp

Product length

退火温度/℃

Annealing temperature

aac（6′）-ⅡF：TTGCGATGCTCTATGAGTGGCTA48052
R：CTCGAATGCCTGGCGTGTTT
aph（3'）-ⅡaF：TGACTGGGCACAACAGACA71754
R：TCAAGAAGGCGATAGAAGGC
aph（3'）-ⅡF：TCTGAAACATGGCAAAGGTAG58254
R：AGCCGTTTCTGTAATGAAGGA
rmtCF：CGAAGAAGTAACAGCCAAAG71155
R：ATCCCAACATCTCTCCCACT
tet （A）F：GCGCCTTTCCTTTGGGTTCT83151
R：CCACCCGTTCCACGTTGTTA
tet （B）F：CATTAATAGGCGCATCGCTG93055
R：TGAAGGTCATCGATAGCAGG
tet （C）F：GCTGTAGGCATAGGCTTGGT88855
R：GCCGGAAGCGAGAAGAATCA
tet （X）F：ATGACAATGCGAATAGATACAGAC1 16753
R：CAATTGCTGAAACGTAAAGTC
blaSHVF：CACTCAAGGATGTATTGTG88555
R：TTAGCGTTGCCAGTGCTCG
blaDHAF：AACTTTCACAGGTGTGCTGT38756
R：CCGTACGCATACTGGCTTTC
qnrAF：ATTTCTCACGCCAGGATTTG51658
R：GATCGGCAAAGGTTAGGTCA
qnrBF：GATCGTGAAAGCCAGAAAGG46958
R：ACGATGCCTGGTAGTTGTCC
qnrSF：ACGACATTCGTCAACTGCAA41753
R：TAAATTGGCACCCTGTAGGC
Sul1F：GTGACGGTGTTCGGCATTCT77968
R：CCGAGAAGGTGATTGCGCT
Sul2F：CCTGTTTCGTCCGACACAGA43551
R：GAAGCGCAGCCGCAATTCAT
FloRF：CTGAGGGTGTCGTCATCTAC67358
R：GCTCCGACAATGCTGACTAT
), ArticleFig(id=1307286565236007859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, label=Table 2, caption=

Sequence information of virulence gene primers

, figureFileSmall=null, figureFileBig=null, tableContent=

目的基因

Target genes

引物序列（5'→3'）

Primer sequences

产物大小/bp

Product length

退火温度/℃Annealing temperature
luxCF：CGCGGATCCATGAATATCAGTATTAAAATTTC1 03552
R：CGCGTCGACTTAGTCTATGGTTCTTAGAAAAG
luxr1F：CGCGGATCCATGGAAGATATCAATGGTTT75952
R：CGCGTCGACTTAAATCAGTTTATTAATTTTAGC
AMTF：CGCGGATCCATGAATATGTTTTTGTAGACG25852
R：CGCGTCGACTCAATCATAATGAAATCTGC
luxEF：CGCGGATCCATGCCTTCTATTTTTGATATTA99952
R：CGCGTCGACCTAAGAAACCAAAAGGCTAC
IS1F：CGCGGATCCATGATAGAAATTAAGACCTTG65452
R：CGCGTCGACCTAATACCAAAAATATATTTTC
tctRF：CGCGGATCCATGAGCAACAGGATATTATT68752
R：CGCGTCGACTTAATTTTTAACTAGAAGCC
lipidAF：CGCGGATCCATGCTAAATAATATCCTTTT88552
R：CGCGTCGACTTTATTGTTTAGCAAAAGTT
TRF：CGCGGATCCATGGAAGATATCAATGGTTT75952
R：CGCGTCGACTTAAATCAGTTTATTAATTTTAGC
cwaHF：CGCGGATCCATGAGAAGATTTATTGGTTT52252
R：CGCGTCGACTTAAAGTATTCTTTTTGCAA
CAMPF：ATGAAACAATCTATTATCTTAGGTA1 02655
R：TTACTTTGCATTTAACTCATATC
TbdR1F：TCGTGGGGCAATCTAAACTAAGC42755
R：AGCCCTGCCCAAACATCATAA
wzaF：AGTCTTTAGACAAGTAGATGCC93650
R：TTACTTTGCATTTAACTCATATC
AS87-0405F：AGGCCGCTTTAGGTTCTGTTC58555
R：CTTCGCCTATTTTCTCATCGTATTT
SIPF：ATGCCTAAGACACCGAAATGGATG72955
R：TTACAAACCTTGTTTTCCTTCCACC
luxr2F：CGCGGATCCATGGGGGATTTTGACGGT75952
R：CGCGTCGACTTACACTAATTTATTAATTTTAG
FurF：ATGGAACATCAAGAGAAAGATATAG46849
R：TTATGCTTTTTTATGACCGTAGA
), ArticleFig(id=1307286565328282548, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, label=表2, caption=

毒力基因引物序列信息

, figureFileSmall=null, figureFileBig=null, tableContent=

目的基因

Target genes

引物序列（5'→3'）

Primer sequences

产物大小/bp

Product length

退火温度/℃Annealing temperature
luxCF：CGCGGATCCATGAATATCAGTATTAAAATTTC1 03552
R：CGCGTCGACTTAGTCTATGGTTCTTAGAAAAG
luxr1F：CGCGGATCCATGGAAGATATCAATGGTTT75952
R：CGCGTCGACTTAAATCAGTTTATTAATTTTAGC
AMTF：CGCGGATCCATGAATATGTTTTTGTAGACG25852
R：CGCGTCGACTCAATCATAATGAAATCTGC
luxEF：CGCGGATCCATGCCTTCTATTTTTGATATTA99952
R：CGCGTCGACCTAAGAAACCAAAAGGCTAC
IS1F：CGCGGATCCATGATAGAAATTAAGACCTTG65452
R：CGCGTCGACCTAATACCAAAAATATATTTTC
tctRF：CGCGGATCCATGAGCAACAGGATATTATT68752
R：CGCGTCGACTTAATTTTTAACTAGAAGCC
lipidAF：CGCGGATCCATGCTAAATAATATCCTTTT88552
R：CGCGTCGACTTTATTGTTTAGCAAAAGTT
TRF：CGCGGATCCATGGAAGATATCAATGGTTT75952
R：CGCGTCGACTTAAATCAGTTTATTAATTTTAGC
cwaHF：CGCGGATCCATGAGAAGATTTATTGGTTT52252
R：CGCGTCGACTTAAAGTATTCTTTTTGCAA
CAMPF：ATGAAACAATCTATTATCTTAGGTA1 02655
R：TTACTTTGCATTTAACTCATATC
TbdR1F：TCGTGGGGCAATCTAAACTAAGC42755
R：AGCCCTGCCCAAACATCATAA
wzaF：AGTCTTTAGACAAGTAGATGCC93650
R：TTACTTTGCATTTAACTCATATC
AS87-0405F：AGGCCGCTTTAGGTTCTGTTC58555
R：CTTCGCCTATTTTCTCATCGTATTT
SIPF：ATGCCTAAGACACCGAAATGGATG72955
R：TTACAAACCTTGTTTTCCTTCCACC
luxr2F：CGCGGATCCATGGGGGATTTTGACGGT75952
R：CGCGTCGACTTACACTAATTTATTAATTTTAG
FurF：ATGGAACATCAAGAGAAAGATATAG46849
R：TTATGCTTTTTTATGACCGTAGA
), ArticleFig(id=1307286565428945845, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, label=Table 3, caption=

Information of clinical isolates of Riemerella anatipestifer

, figureFileSmall=null, figureFileBig=null, tableContent=

序号

No.

地区

District

分离时间

Isolation time

家禽品种

Breed

日龄/d

Age （Day）

血清型

Serotype

1福建2022-09肉鸭28214
2山东2022-10肉鸭346
3山东2022-10肉鸭2911
4山东2022-10肉鸭3313
5河北2022-10肉鸭2712
6河北2022-11肉鸭3210
7广东2022-11肉种鸡564
8广西2022-12肉鸭3216
9广东2023-01鹅181
10山东2023-02肉鸭2415
11广西2023-12肉鸭205
12山东2022-09肉鸭2717
13河北2023-08蛋鸡2507
14山东2023-05肉鸭249
15福建2023-12肉鸭182
), ArticleFig(id=1307286565571552182, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, label=表3, caption=

鸭疫里默氏杆菌临床分离菌株信息表

, figureFileSmall=null, figureFileBig=null, tableContent=

序号

No.

地区

District

分离时间

Isolation time

家禽品种

Breed

日龄/d

Age （Day）

血清型

Serotype

1福建2022-09肉鸭28214
2山东2022-10肉鸭346
3山东2022-10肉鸭2911
4山东2022-10肉鸭3313
5河北2022-10肉鸭2712
6河北2022-11肉鸭3210
7广东2022-11肉种鸡564
8广西2022-12肉鸭3216
9广东2023-01鹅181
10山东2023-02肉鸭2415
11广西2023-12肉鸭205
12山东2022-09肉鸭2717
13河北2023-08蛋鸡2507
14山东2023-05肉鸭249
15福建2023-12肉鸭182
), ArticleFig(id=1307286565680604087, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, label=Table 4, caption=

Drug susceptibility test results of isolated strains

, figureFileSmall=null, figureFileBig=null, tableContent=

药物名称

Drug name

血清型Serotype

耐药率/%

Drug resistance rate

12456791011121314151617

链霉素

Streptomycin

SRRSRSSSSSSRSSS26.67

庆大霉素

Gentamicin

SSRSSSSSSSSSRSS13.33

多西环素

Doxycycline

SSIRRSSSISSSSSS13.33

替加环素

Tigecycline

SSSSSSSSSSSSSSS0

氨苄西林

Ampicillin

RRRSRRRIRRRRRRS80

阿莫西林

Amoxicillin

RRRRRRRRRRRRIRR93.33

恩诺沙星

Enrofloxacin

SSSSSISSISRSRSI13.33

环丙沙星

Ciprofloxacin

SSSSSIRSISISISS6.67

复方新诺明

Co-trimoxazole

SRRSRRRRSRSRRRR73.33

氯霉素

Chloramphenicol

SIISSSSSSISSRSS6.67
), ArticleFig(id=1307286565756101560, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, label=表4, caption=

分离菌的药敏试验结果

, figureFileSmall=null, figureFileBig=null, tableContent=

药物名称

Drug name

血清型Serotype

耐药率/%

Drug resistance rate

12456791011121314151617

链霉素

Streptomycin

SRRSRSSSSSSRSSS26.67

庆大霉素

Gentamicin

SSRSSSSSSSSSRSS13.33

多西环素

Doxycycline

SSIRRSSSISSSSSS13.33

替加环素

Tigecycline

SSSSSSSSSSSSSSS0

氨苄西林

Ampicillin

RRRSRRRIRRRRRRS80

阿莫西林

Amoxicillin

RRRRRRRRRRRRIRR93.33

恩诺沙星

Enrofloxacin

SSSSSISSISRSRSI13.33

环丙沙星

Ciprofloxacin

SSSSSIRSISISISS6.67

复方新诺明

Co-trimoxazole

SRRSRRRRSRSRRRR73.33

氯霉素

Chloramphenicol

SIISSSSSSISSRSS6.67
), ArticleFig(id=1307286565990982585, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, label=Table 5, caption=

Detection rate of resistance genes of isolated strains

, figureFileSmall=null, figureFileBig=null, tableContent=

耐药基因

Resistance gene

血清型Serotype

检出率/%

Detection rate

12456791011121314151617
aac（6'）-Ⅱ+6.67
aph（3'）-ILa
aph（3'）-Ⅱ+++++++++++73.33
rmtC
tet （A）
tet （B）
tet （C）+6.67
tet（X）++++++++++++++93.33
blaSHV
blaDHA
qnrA
qnrB+6.67
qnrS
Sul1+6.67
Sul2+6.67
floR++++++++++++80
合计 Total224223333244233
), ArticleFig(id=1307286566095840186, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, label=表5, caption=

分离菌耐药基因检测结果

, figureFileSmall=null, figureFileBig=null, tableContent=

耐药基因

Resistance gene

血清型Serotype

检出率/%

Detection rate

12456791011121314151617
aac（6'）-Ⅱ+6.67
aph（3'）-ILa
aph（3'）-Ⅱ+++++++++++73.33
rmtC
tet （A）
tet （B）
tet （C）+6.67
tet（X）++++++++++++++93.33
blaSHV
blaDHA
qnrA
qnrB+6.67
qnrS
Sul1+6.67
Sul2+6.67
floR++++++++++++80
合计 Total224223333244233
), ArticleFig(id=1307286566175531963, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=EN, label=Table 6, caption=

Detection rate of Virulence genes of isolated strains

, figureFileSmall=null, figureFileBig=null, tableContent=

毒力基因

Virulence genes

血清型 Serotype

检出率/%

Detectionrate

12456791011121314151617
luxC+6.67（1/15）
luxr+++20.00（3/15）
AMT++++26.67（4/15）
luxE++++++++53.33（8/15）
ISl+++++++46.67（7/15）
tctR+++++++++++++86.67（13/15）
lipidA+++++++46.67（7/15）
TR+++++++++++73.33（11/15）
cwaH+++++++46.67（7/15）
CAMP++++++++++++80（12/15）
TbdR1+++++++++++++86.67（13/15）
wza+++++33.33（5/15）
AS87-0405++++++++53.33（8/15）
SIP+++++++++++73.33（11/15）
luxr2+++20.00（3/15）
Fur+++++++46.67（7/15）
合计Total6554118913981312188
), ArticleFig(id=1307286566263612348, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, language=CN, label=表6, caption=

分离菌毒力基因检测结果

, figureFileSmall=null, figureFileBig=null, tableContent=

毒力基因

Virulence genes

血清型 Serotype

检出率/%

Detectionrate

12456791011121314151617
luxC+6.67（1/15）
luxr+++20.00（3/15）
AMT++++26.67（4/15）
luxE++++++++53.33（8/15）
ISl+++++++46.67（7/15）
tctR+++++++++++++86.67（13/15）
lipidA+++++++46.67（7/15）
TR+++++++++++73.33（11/15）
cwaH+++++++46.67（7/15）
CAMP++++++++++++80（12/15）
TbdR1+++++++++++++86.67（13/15）
wza+++++33.33（5/15）
AS87-0405++++++++53.33（8/15）
SIP+++++++++++73.33（11/15）
luxr2+++20.00（3/15）
Fur+++++++46.67（7/15）
合计Total6554118913981312188
)], attaches=null, journal=Journal(id=1306267422417846359, delFlag=0, nameCn=畜牧兽医学报, nameEn=Acta Veterinaria et Zootechnica Sinica, nameHistory1=null, nameHistory2=null, issn=0366-6964, eissn=null, cn=11-1985/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=lkbuTWCUJ7iVBIRfH50Jkw==, journalPrice=null, startedYear=null, abbrevIsoEn=Acta Veterinaria et Zootechnica Sinica, journalRemark=null, publicationField=null, createdTime=1789368022782, updatedTime=1789370363065, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=X, firstLetterEn=X, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=lkbuTWCUJ7iVBIRfH50Jkw==, picEn=lpjFQiQXDp8Dj5mnLAs9og==, jcr=null, cjcr=null, exts=[JournalExt(id=1306277238341656590, language=CN, name=畜牧兽医学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789370363078, updatedTime=1789370363078, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://www.xmsyxb.com/JournalX_xmsy/authorLogOn.action, submissionEditorUrl=https://www.xmsyxb.com/JournalX_xmsy/editorLogOn.action, submissionReviewUrl=https://www.xmsyxb.com/JournalX_xmsy/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1306277238396182543, language=EN, name=Acta Veterinaria et Zootechnica Sinica, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789370363091, updatedTime=1789370363091, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://www.xmsyxb.com/JournalX_xmsy/authorLogOn.action, submissionEditorUrl=https://www.xmsyxb.com/JournalX_xmsy/editorLogOn.action, submissionReviewUrl=https://www.xmsyxb.com/JournalX_xmsy/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1306274286668972124, websiteList=[Website(id=1306284065666130885, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1306274286668972124, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xmsyxb/CN, language=CN, createTime=1789371990840, createBy=18614031015, updateTime=1789373819393, updateBy=18614031015, name=畜牧兽医学报-中文, tplId=1146099689490845704, title=畜牧兽医学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1306291866169790796, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=articleTextType, value=kx, createTime=1789373850624, updateTime=1789373850624, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866144624969, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=banner, value=null, createTime=1789373850618, updateTime=1789373850618, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866186568015, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=grayFlag, value=0, createTime=1789373850628, updateTime=1789373850628, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866136236360, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=logo, value=https://castjournals.cast.org.cn/joweb/xmsyxb/CN/file/pic?fileId=UW9FSLxGySpUV3G8KWC+Vw==, createTime=1789373850616, updateTime=1789373850616, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866203345233, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=minRunFlag, value=0, createTime=1789373850632, updateTime=1789373850632, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866161402187, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xmsyxb/CN/file/pic, createTime=1789373850622, updateTime=1789373850622, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866194956624, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=silenceFlag, value=0, createTime=1789373850630, updateTime=1789373850630, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866153013578, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1789373850620, updateTime=1789373850620, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866173985101, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=themeColor, value=null, createTime=1789373850625, updateTime=1789373850625, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291866182373710, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065666130885, code=themeStyle, value=null, createTime=1789373850627, updateTime=1789373850627, creator=18614031015, updator=18614031015)]), Website(id=1306284065745822666, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1306274286668972124, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xmsyxb/EN, language=EN, createTime=1789371990858, createBy=18614031015, updateTime=1789373814653, updateBy=18614031015, name=畜牧兽医学报-英文, tplId=1146101810881728533, title=Acta Veterinaria et Zootechnica Sinica, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1306291829457047871, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=articleTextType, value=kx, createTime=1789373841871, updateTime=1789373841871, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829431882044, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=banner, value=null, createTime=1789373841865, updateTime=1789373841865, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829478019394, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=grayFlag, value=0, createTime=1789373841876, updateTime=1789373841876, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829419299131, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=logo, value=https://castjournals.cast.org.cn/joweb/xmsyxb/EN/file/pic?fileId=UW9FSLxGySpUV3G8KWC+Vw==, createTime=1789373841862, updateTime=1789373841862, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829490602308, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=minRunFlag, value=0, createTime=1789373841879, updateTime=1789373841879, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829448659262, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xmsyxb/EN/file/pic, createTime=1789373841869, updateTime=1789373841869, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829486408003, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=silenceFlag, value=0, createTime=1789373841878, updateTime=1789373841878, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829440270653, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1789373841867, updateTime=1789373841867, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829465436480, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=themeColor, value=null, createTime=1789373841873, updateTime=1789373841873, creator=18614031015, updator=18614031015), WebsiteProps(id=1306291829469630785, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1306284065745822666, code=themeStyle, value=null, createTime=1789373841874, updateTime=1789373841874, creator=18614031015, updator=18614031015)])], journalTitle=畜牧兽医学报, weixinUrl=null, journalUrl=https://www.xmsyxb.com/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Acta Veterinaria et Zootechnica Sinica, journalPhotoCn=lkbuTWCUJ7iVBIRfH50Jkw==, journalPhotoEn=lpjFQiQXDp8Dj5mnLAs9og==, journalFirstLetter=X, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/xmsyxb/CN/10.11843/j.issn.0366-6964.2026.01.031, detailUrlEn=https://castjournals.cast.org.cn/joweb/xmsyxb/EN/10.11843/j.issn.0366-6964.2026.01.031, pdfUrlCn=https://castjournals.cast.org.cn/joweb/xmsyxb/CN/PDF/10.11843/j.issn.0366-6964.2026.01.031, pdfUrlEn=https://castjournals.cast.org.cn/joweb/xmsyxb/EN/PDF/10.11843/j.issn.0366-6964.2026.01.031, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=1, orderTime=1769097600000, fullTextJson=null, articleText=null, reference=null)
收藏切换
15种血清型的鸭疫里默氏杆菌毒力基因检测和耐药性分析
收藏切换
PDF下载
李晨阳 1 , 张传美 2 , 惠宇韬 2 , 吕莹 2 , 杨敏 1
畜牧兽医学报 | 预防兽医 2026,57(1): 360-368
收起
收藏切换
畜牧兽医学报 |预防兽医 2026 , 57 (1) : 360 -368
15种血清型的鸭疫里默氏杆菌毒力基因检测和耐药性分析
全屏
[Author(id=1307286561163338639, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=1003354610@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561234641809, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561163338639, language=EN, stringName=Chenyang LI, firstName=Chenyang, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286561377248146, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561163338639, language=CN, stringName=李晨阳, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.甘肃农业大学动物医学院，兰州 730070, bio={"content":"

李晨阳，博士，讲师，主要从事动物病原菌的耐药性研究，E-mail：

"}, bioImg=null, bioContent=

李晨阳，博士，讲师，主要从事动物病原菌的耐药性研究，E-mail：

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286560957817736, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=1., ext=[AuthorCompanyExt(id=1307286560991372169, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China), AuthorCompanyExt(id=1307286561003955082, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.甘肃农业大学动物医学院，兰州 730070)])]), Author(id=1307286561444357012, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561524048790, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561444357012, language=EN, stringName=Chuanmei ZHANG, firstName=Chuanmei, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286561591157655, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561444357012, language=CN, stringName=张传美, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛农业大学动物医学院，青岛 266109, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286561083646859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=2., ext=[AuthorCompanyExt(id=1307286561092035468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China), AuthorCompanyExt(id=1307286561100424077, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛农业大学动物医学院，青岛 266109)])]), Author(id=1307286561662460825, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561737958299, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561662460825, language=EN, stringName=Yutao HUI, firstName=Yutao, middleName=null, lastName=HUI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286561809261468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561662460825, language=CN, stringName=惠宇韬, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛农业大学动物医学院，青岛 266109, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286561083646859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=2., ext=[AuthorCompanyExt(id=1307286561092035468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China), AuthorCompanyExt(id=1307286561100424077, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛农业大学动物医学院，青岛 266109)])]), Author(id=1307286561872176030, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307286561943479200, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561872176030, language=EN, stringName=Ying LÜ, firstName=Ying, middleName=null, lastName=LÜ, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286562002199457, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286561872176030, language=CN, stringName=吕莹, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛农业大学动物医学院，青岛 266109, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286561083646859, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=2., ext=[AuthorCompanyExt(id=1307286561092035468, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China), AuthorCompanyExt(id=1307286561100424077, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286561083646859, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛农业大学动物医学院，青岛 266109)])]), Author(id=1307286562094474147, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=yummy12@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1307286562207720357, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286562094474147, language=EN, stringName=Min YANG, firstName=Min, middleName=null, lastName=YANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307286562279023526, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, authorId=1307286562094474147, language=CN, stringName=杨敏, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.甘肃农业大学动物医学院，兰州 730070, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307286560957817736, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, xref=1., ext=[AuthorCompanyExt(id=1307286560991372169, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China), AuthorCompanyExt(id=1307286561003955082, tenantId=1146029695717560320, journalId=1306274286668972124, articleId=1307007628861796566, companyId=1307286560957817736, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.甘肃农业大学动物医学院，兰州 730070)])])]
李晨阳1 , 张传美2, 惠宇韬2, 吕莹2, 杨敏1
作者信息
  • 1.甘肃农业大学动物医学院，兰州 730070
  • 2.青岛农业大学动物医学院，青岛 266109
通讯作者:
杨敏，主要从事药物化学研究，E-mail：
作者简介:

李晨阳，博士，讲师，主要从事动物病原菌的耐药性研究，E-mail：

Detection of Virulence Genes and Drug Resistance Analysis of 15 Serotypes of Riemerella anatipestifer
Chenyang LI1 , Chuanmei ZHANG2, Yutao HUI2, Ying LÜ2, Min YANG1
Affiliations
  • 1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China
  • 2.College of Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China
    • Corresponding Author:
    YANG Min， E-mail：
出版时间: 2026-01-23 doi: 10.11843/j.issn.0366-6964.2026.01.031
文章导航
收藏切换
摘要
收起

旨在了解我国部分地区鸭疫里默氏杆菌分离株的耐药性及毒力基因分布情况，采用K-B法测定15种血清型鸭疫里默氏杆菌对10种临床常用抗菌药物的敏感性，同时采用PCR技术对不同血清型RA分离株进行了16种耐药基因和16种毒力基因的检测。结果表明，菌株对阿莫西林的耐药率为93.33%，对氨苄西林的耐药率为80%；耐药基因检测结果显示，四环素类的tet（X）基因、酰胺醇类的floR基因、氨基糖苷类的aph（3'）-Ⅱ基因的检出率分别为93.33%、80%、73.33%。毒力基因检测结果显示，tctR、TbdR1、TR、CAMP和AS87-0405毒力基因检出率最高，分别为86.67%、86.67%、80%、73.33%、73.33%。这些分离株对四环素类、喹诺酮类、酰胺醇类药物较敏感，对β-内酰胺类药物具有较高的耐药性；同时这些分离株携带多种毒力基因，可能预示着其具有较强毒力。以上结果为我国部分地区鸭疫里默氏杆菌病防控提供了一定的参考依据。

鸭疫里默氏杆菌  /  毒力基因  /  耐药基因  /  耐药性

In order to understand the drug resistance and virulence gene distribution of Riemerella anatipestifer isolates in some areas of China， K-B method was used to determine the sensitivity of 15 serotypes of R. anatipestifer to 10 commonly used antibiotics， and PCR technology was used to detect 16 drug resistance genes and 16 virulence genes of different serotypes of R. anatipestifer isolates. The results showed that the resistance rates of amoxicillin and ampicillin were 93.33% and 80%， respectively. The detection results of drug-resistant genes showed that the detection rates of tet（X） gene of tetracycline， floR gene of amide alcohol and aph（3'）-Ⅱ gene of aminoglycoside were 93.33%， 80% and 73.33% respectively. The results of virulence gene detection showed that tctR， TbdR1， TR， CAMP and AS87-0405 had the highest detection rates， which were 86.67%， 86.67%， 80%， 73.33% and 73.33% respectively. These isolates are sensitive to tetracyclines， quinolones and amide alcohols， and have high resistance to β-lactams. At the same time， these isolates carry a variety of virulence genes， which may indicate that they have strong virulence. The above results provide some reference for the prevention and control of R. anatipestifer in some areas of China.

Riemerella anatipestifer  /  virulence gene  /  drug resistance gene  /  drug-fast
李晨阳, 张传美, 惠宇韬, 吕莹, 杨敏. 15种血清型的鸭疫里默氏杆菌毒力基因检测和耐药性分析. 畜牧兽医学报, 2026 , 57 (1) : 360 -368 . DOI: 10.11843/j.issn.0366-6964.2026.01.031
Chenyang LI, Chuanmei ZHANG, Yutao HUI, Ying LÜ, Min YANG. Detection of Virulence Genes and Drug Resistance Analysis of 15 Serotypes of Riemerella anatipestifer[J]. Acta Veterinaria et Zootechnica Sinica, 2026 , 57 (1) : 360 -368 . DOI: 10.11843/j.issn.0366-6964.2026.01.031
正文
收起
鸭疫里默氏杆菌（Riemerella anatipestifer，RA）是引起鸭、鹅等水禽鸭疫里默氏菌病的病原菌，近年来也有引起鸡群发病的报道［1］。1932年，美国学者在纽约长岛首次发现了鸭疫里默氏杆菌，并将之引起的鸭病称为“新鸭病”［2］。我国于1975年首次报道该病存在［3］。1982年，郭玉璞等［4］首次在国内分离到该病原菌。鸭疫里默氏杆菌引起的疾病具有高死亡率、高淘汰率等特点，对养鸭业危害巨大，已成为对养鸭业危害最为严重的传染病之一［5］。
鸭疫里默氏杆菌的血清型众多，目前分离鉴定的血清型有21种［6⁃8］；我国目前已经报道的血清型有18种［9⁃10］。由于鸭疫里默氏杆菌各个血清型之间缺乏交叉保护作用，疫苗防控具有一定的局限性，因此采用药物治疗尤为重要。但近年来细菌耐药性问题越来越严重，许多致病菌株已产生多重耐药性，不仅给禽类养殖业用药方案造成了较大困扰，也给人类公共健康带来了潜在的威胁。基于减抗、禁抗的养殖大环境，科学用药在鸭疫里默氏杆菌病的防治中的地位更加突出。
本研究通过对我国部分地区鸭疫里默氏杆菌分离株的药敏试验、耐药基因检测，为该病原菌的合理用药和科学防治提供参考依据。同时，本研究也对该病原菌分离株的毒力基因进行了检测，为探索该病原菌的致病机理提供了基础数据。
1　材料与方法
收起
1.1　试验菌株
2022年9月—2023年12月，自山东、福建、广东、河北、广西五省分离到15种血清型的鸭疫里默氏杆菌，分别是1、2、4、5、6、7、9、10、11、12、13、14、15、16、17型。
采用玻片凝集试验和琼脂扩散沉淀试验两种方法同时对RA分离株进行血清型鉴别，血清型分型鉴别工作由四川农业大学朱德康教授完成。
1.2　主要试剂
TSA（大豆酪蛋白琼脂培养基）、TSB（胰蛋白胨大豆液体培养基）和革兰氏染色液均购自青岛海博生物技术有限公司；绿色荧光核酸染料购自青岛睿博兴科生物技术有限公司；新生牛血清、50×TAE缓冲液购自北京索莱宝科技有限公司；药敏纸片购自常德比克曼生物科技有限公司；细菌基因组DNA 提取试剂盒购自天根生化科技有限公司；DNA Marker购自南京诺维赞生物技术有限公司。
1.3　鸭疫里默氏杆菌鉴定引物
根据GenBank中发表的鸭疫里默氏杆菌16S rRNA基因序列设计引物，扩增片段大小为475 bp［11］。上游引物RA-001F：5′-TTAGATAG- TTGGTGAGGTAA-3′。下游引物RA-002R：5′- ATCGGTGTTCTGAGTAAT-3′。引物由生工生物青岛有限公司合成。
1.4　耐药基因和毒力基因的检测引物
根据文献［12⁃14］和GenBank上已公布的序列，确定16种耐药基因和16种毒力基因的引物序列，详见表1、2。
1.5　细菌分离鉴定
1.5.1　细菌分离
无菌取病死鸭的脑组织，在含5%新生牛血清的TSA培养基上划线接种，置5%~10%CO2的条件下，37 ℃培养24~48 h，挑取典型菌落（圆形、微隆起、表面光滑、直径1~2 mm无色素菌落，革兰染色为短杆状、杆状或丝状革兰阴性菌）纯化1代后保存，并按以下步骤进行鉴定。
1.5.2　PCR鉴定
1.5.2.1　模板制备：挑取待检菌株典型单菌落，溶于100 μL去离子水中，沸水浴5 min，瞬时离心后作为待检模板；挑取已鉴定为鸭疫里默氏杆菌菌株典型单菌落，溶于100 μL去离子水中，沸水浴5 min，瞬时离心后作为阳性对照模板；取去离子水，均立即使用或-15 ℃以下保存备用。
1.5.2.2　PCR检测：采用25 μL扩增体系。扩增体系：模板5.0 μL，2×PCR Premix 12.5 μL，上游引物1.0 μL（50 pmol），下游引物1.0 μL（50 pmol），去离子水5.5 μL，瞬时离心备用。反应程序：94 ℃预变性5 min；94 ℃变性30 s，54 ℃退火30 s，72 ℃延伸30 s，30个循环；72 ℃延伸10 min。扩增结束后，取扩增产物10.0 μL，点样于1%琼脂糖凝胶电泳孔中，以150~200 V电压于1倍TAE电泳缓冲液中电泳，用凝胶成像仪观察结果。
结果判定：阳性对照应出现475 bp的目的条带，而阴性对照应无此条带。待检样品出现与阳性对照位置一致的条带，则判定为阳性；否则判定为阴性。
1.6　菌株耐药性检测
参照美国临床和实验室标准协会（Clinical ＆ Laboratory Standards Institute）的Kirby-Bauer法，结合临床用药实际，对鸭疫里默氏杆菌分离株进行阿莫西林、氨苄西林、恩诺沙星等10种药物的敏感性试验，测量抑菌圈直径，根据药敏纸片使用说明书和CLSI的药敏判定标准，分别以R（耐药）、I（中介）、S（敏感）对其耐药性进行评价。参照Magiorakos等［11］对多重耐药性的定义，对3种及以上药物耐药定义为多药耐药菌株［15］。
1.7　耐药基因和毒力基因的检测
根据文献［12⁃14］方法进行鉴定。
2　结果
收起
2.1　细菌分离鉴定
经过分离、纯化、PCR鉴定和血清型分型，确认分离菌株为鸭疫里默氏杆菌。菌株背景信息、鉴定结果详见表3，部分PCR鉴定结果见图1。
2.2　耐药性检测
15种不同血清型鸭疫里默氏杆菌对临床常用的10种抗菌药物的耐药情况见表4。可以看出，分离菌株对10种抗菌药物存在不同程度的耐药性。其中，对阿莫西林耐药率最高，达到93.33%，其次，对氨苄西林（80%）、复方新诺明（73.33%）、链霉素（26.67%）的耐药率也较高，而对四环素类、喹诺酮类、酰胺醇类药物则比较敏感。
2.3　耐药基因检测
对15种不同血清型的鸭疫里默氏杆菌进行16种耐药基因检测，结果如表5所示。其中，tet（X）、floR、aph（3'）-Ⅱ 三种耐药基因的检出率均大于70%，分别为93.33%、80%、73.33%。aac（6′）-Ⅱ、tet（C）、qnrB、Sul1、Sul2五种耐药基因的检出率均为6.67%，其余未检出。
2.4　毒力基因检测
对分离的15种不同血清型的鸭疫里默氏杆菌进行16种毒力基因检测，结果如表6所示。其中，毒力基因tctR、CAMP、TbdR1分别高达86.67%、80%、86.67%；毒力基因TR、SIP的检出率为73.33%、73.33%；毒力基因luxC的检出率最低，仅为6.67%。此外，血清型10型、13型鸭疫里默氏杆菌携带毒力基因数量最多，均为13种，14型检出12种，6型检出11种，5型鸭疫里默氏杆菌毒力基因检出数量最少，仅检出4种。
3　讨论
收起
药敏试验结果显示，本研究中鸭疫里默氏杆菌普遍对阿莫西林、氨苄西林、复方新诺明和链霉素等药物具有一定的耐药性，而对四环素类、喹诺酮类、酰胺醇类药物的耐药率较低。所以在临床用药要尽量避免使用阿莫西林、氨苄西林、复方新诺明和链霉素等药物。
本研究中，16种耐药基因检测出8种，耐药表型与耐药基因的相关性不尽相同。本研究中鸭疫里默氏杆菌对β-内酰胺类药物的耐药表型为高度耐药，而所有分离株均未检测出β-内酰胺类耐药基因bla SHV、bla DHA，这可能是菌株通过其他机制介导耐药或同类的其他耐药基因介导有关［16］。此外，RA分离株对磺胺类药物复方新诺明耐药率为73.33%，而磺胺类耐药基因中，sul 1和sul 2检出率均为6.67%，由此可以推断，β-内酰胺类、磺胺类耐药基因阳性率与耐药率的相关性较差，这可能是基因隐性表达、基因不表达或其他外界环境因素所致。值得注意的是，15种不同血清型鸭疫里默氏杆菌tet（X）耐药基因阳性率高达93.33%（14/15），tet（X）是一种黄素依赖的单加氧酶，可以在有氧和NADPH的作用下对四环素进行化学修饰，使其失去活性。这种修饰作用包括临床上应用的替加环素等四环素类抗生素。有研究发现，RA可以携带多种tet型耐药基因，并且可能是tet（X）基因的源头宿主，同时扮演着tet（X）家族传播至临床病原菌的关键角色［17⁃18］。不同亚型的tet（X）基因可能从鸭传播到其他动物以及人类身上，对于养殖场从业人员来说，长期暴露在携带tet（X）细菌污染的环境中可能存在潜在风险。
鸭疫里默氏杆菌的致病性与其携带的毒力基因种类和数量密切相关。本研究选取16种毒力相关基因进行鉴定，16种基因全部被检出。其中，tctR、CAMP、TbdR1、TR、SIP检出率在70%以上。血清型6型、10型、13型、14型检出的毒力基因数量分别是11种、13种、13种、12种，不同血清型致病性强弱是否因为这些毒力基因共同作用的结果还有待研究。
4　结论
收起
本研究中的15种血清型鸭疫里默氏杆菌对β-内酰胺类药物耐药性强，分离菌株毒力基因种类多样，tet（X）耐药基因检出率高。基于tet（X）在鸭疫里默氏杆菌中的高度流行，有必要对其通过鸭疫里默氏杆菌传播到其他临床病原菌的可能性进一步研究。

文献
收起
参考文献 引证文献
排序方式：
[1]
李富祥，赵文华，宋建领，等.鸡源鸭疫里默杆菌的分离鉴定及ompA基因遗传进化分析［J］.中国兽医学报，2021，41（2）：253-258.
LI F X，ZHAO W H，SONG J L，et al.Isolation and identification of Riemerella anatipestifer from Chickens and genetic evolutionary analysis of its ompA gene.1［J］.Chinese Journal of Veterinary Medicine，2021，41（2）：253-258.（in Chinese）
[2]
CHEN S，ZENG X，ZHENG X，et al.Identification of a highly pathogenic Riemerella anatipestifer strain causing duck spleen marble-like necrosis disease in China［J］.Microb Pathog，2024，196：106912.
[3]
欧芳杏，张 霞，陈佳琪，等.2021—2023年贵州省鸭疫里默氏杆菌病流行病学调查［J］.中国畜禽种业，2024，20（6）：110-119.
OU F X，ZHANG X，CHEN J Q，et al.Epidemiological survey of Riemerella anatipestifer disease in guizhou province from 2021 to 2023［J］.Chinese Journal of Livestock and Poultry Breeding，2024，20（6）：110-119.（in Chinese）
[4]
郭玉璞，陈德威，范国雄，等.北京鸭小鸭传染性浆膜炎的调查研究［J］.畜牧兽医学报，1982，2：35- 41.
GUO Y P，CHEN D W，FAN G X，et al.Investigation and study on infectious serositis in ducklings of Peking duck［J］.Acta Veterinaria et Zootechnica Sinica，1982，2：35-41.（in Chinese）
[5]
YANG Z，YANG X，WANG M，et al.Genome-wide association study reveals serovar-associated genetic loci in Riemerella anatipestifer［J］.BMC Genomics，2024，25（1）：57-57.
[6]
RYLL M，HINZ K H.Exclusion of strain 670/89 as type strain for serovar 20 of Riemerella anatipestifer［J］.Berl Munch Tierarztl Wochenschr，2000，113（2）：65-66.
[7]
PATHANASOPHON P，PHUEKTES P，TANTICHAROENYOS T，et al.A potential new serotype of Riemerella anatipestifer isolated from ducks in Thailand［J］.Avian Pathol，2002，31（3）：267-270.
[8]
PATHANASOPHON P，SAWADA T，TANTICHAROENYOS T.New serotypes of Riemerella anatipestifer isolated from ducks in Thailand［J］.Avian Pathol，1995，24（1）：195-199.
[9]
陈国权，吴征卓，姚碧琼，等．鸭疫里默氏杆菌贵州流行株的分离鉴定及耐药性分析［J］.中国兽医科学，2020，50 （6） ：731-739．
CHEN G Q，WU Z Z，YAO B Q，et al.Isolation，identification and drug resistance analysis of Riemerella anatipestifer epidemic strains in Guizhou［J］.Chinese Veterinary Science，2020，50（6）：731-739.（in Chinese）
[10]
程安春，汪铭书，陈孝跃，等.我国鸭疫里默氏杆菌血清型调查及新血清型的发现和病原特性［J］.中国兽医学报，2003，4：320-323.
CHENG A C，WANG M S，CHEN X Y，et al.Serotype survey of Riemerella anatipestifer in China，discovery of new serotypes and pathogen characteristics［J］.Chinese Journal of Veterinary Medicine，2003，4：320-323.（in Chinese）
[11]
祝希辉，李艳兰，王志伟，等.1株鸭疫里默氏杆菌的全基因组测序及耐药性与遗传进化分析［J］.中国畜牧兽医，2023，50（3）：1140-1149.
ZHU X H，LI Y L，WANG Z W，et al.Whole-genome sequencing of one strain of Riemerella anatipestifer and analysis of its drug resistance and genetic evolution［J］.China Animal Husbandry & Veterinary Medicine，2023，50（3）：1140-1149.（in Chinese）
[12]
张旭财，李燕红，陆毅兴，等.广东肇庆地区鸭疫里氏杆菌耐药性和毒力基因分析及ERIC-PCR分型研究［J］.中国动物传染病学报，2022，30（4）：39-47.
ZHANG X C，LI Y H，LU Y X，et al.Analysis of drug resistance，virulence genes and ERIC-PCR typing of Riemerella anatipestifer in Zhaoqing area，Guangdong province［J］.Chinese Journal of Animal Infectious Diseases，2022，30（4）：39-47.（in Chinese）
[13]
张 燕.鸭疫里默氏杆菌安徽分离株耐药性分析与致病性研究［D］.扬州：扬州大学，2022.
ZHANG Y.Analysis of drug resistance and study on pathogenicity of Riemerella anatipestifer isolates from Anhui［D］.Yangzhou：Yangzhou University，2022.（in Chinese）
[14]
王小兰.鸭疫里默氏杆菌烟酰胺酶基因的鉴定及功能研究［D］.北京：中国农业科学院，2018.
WANG X L.Identification and functional study of the nicotinamidase gene in Riemerella anatipestifer［D］.Beijing：Chinese Academy of Agricultural Sciences，2018.（in Chinese）
[15]
MAGIORAKOS A P，SRINIVASAN A，CAREY R B，et al.Multidrug-resistant，extensively drug-resistant and pandrug-resistant bacteria：an international expert proposal for interim standard definitions for acquired resistance［J］.Clin Microbiol Infect，2012，18（3）：268-281.
[16]
常陈星，刘美含，杨跃飞，等.鸭疫里默氏杆菌氨基糖苷类药物的耐药性分析［J］.黑龙江畜牧兽医，2020，13：101-103，108.
CHANG C X，LIU M H，YANG Y F，et al.Analysis of drug resistance of Riemerella anatipestifer to aminoglycoside antibiotics［J］.Heilongjiang Animal Science and Veterinary Medicine，2020，（13）：101-103，108.（in Chinese）
[17]
ZHU D，LUO H，LIU M，et al.Various profiles of tet genes addition to tet（X） in Riemerella anatipestifer isolates from ducks in China［J］.Front Microbiol，2018，9：585.
[18]
TAO M，WANG J，LI K，et al.Development of signature tagged mutagenesis in Riemerella anatipestifer to identify genes essential for survival and pathogenesis［J］.Vet Microbiol，2020，250：108857.
2026年第57卷第1期
PDF下载
156
70
引用本文
BibTeX
文章信息
doi: 10.11843/j.issn.0366-6964.2026.01.031
  • 接收时间：2025-04-16
  • 首发时间：2026-09-16
  • 出版时间：2026-01-23
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2025-04-16
基金
作者信息
    1.甘肃农业大学动物医学院，兰州 730070
    2.青岛农业大学动物医学院，青岛 266109

通讯作者:

杨敏，主要从事药物化学研究，E-mail：
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/xmsyxb/CN/10.11843/j.issn.0366-6964.2026.01.031
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62662699（运营及技术支持）
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持