Article(id=1238813309860892937, tenantId=1146029695717560320, journalId=1192105938417971205, issueId=1238813307784712441, articleNumber=null, orderNo=null, doi=10.13343/j.cnki.wsxb.20250836, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1762444800000, receivedDateStr=2025-11-07, revisedDate=null, revisedDateStr=null, acceptedDate=1766592000000, acceptedDateStr=2025-12-25, onlineDate=1773285709109, onlineDateStr=2026-03-12, pubDate=1772553600000, pubDateStr=2026-03-04, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1773285709109, onlineIssueDateStr=2026-03-12, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1773285709109, creator=13701087609, updateTime=1773285709109, updator=13701087609, issue=Issue{id=1238813307784712441, tenantId=1146029695717560320, journalId=1192105938417971205, year='2026', volume='66', issue='3', pageStart='961', pageEnd='1466', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1773285708614, creator=13701087609, updateTime=1773291912509, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1238839328915378858, tenantId=1146029695717560320, journalId=1192105938417971205, issueId=1238813307784712441, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1238839328915378859, tenantId=1146029695717560320, journalId=1192105938417971205, issueId=1238813307784712441, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1062, endPage=1073, ext={EN=ArticleExt(id=1238813310238380307, articleId=1238813309860892937, tenantId=1146029695717560320, journalId=1192105938417971205, language=EN, title=Research progress in enzootic nasal tumor virus of sheep and goats, columnId=1192149543727808575, journalTitle=Acta Microbiologica Sinica, columnName=Review, runingTitle=null, highlight=null, articleAbstract=

The enzootic nasal tumor of sheep and goats is a progressive and contagious disease caused by enzootic nasal tumor virus. It is mainly characterized by tumor growth in the mucosal epithelial tissue of the ethmoid bone and nasal turbinates within the nasal cavity of sheep and goats. In the later stage of the disease, the significant enlargement of tumor volume can lead to upper respiratory tract obstruction, which subsequently causes the affected animals to die of asphyxiation. This disease markedly reduces the production performance of infected animals, causing economic losses to the livestock industry. Moreover, it poses a threat to precious local breeds and core breeding flocks, resulting in the loss of high-quality breeding sheep. Consequently, it has become one of the major diseases threatening the sheep and goat industry. Currently, there are no vaccines or specific treatments for this disease. This article reviews enzootic nasal tumor virus in terms of the etiology, epidemiology, clinical symptoms, main pathological changes, diagnosis, and prevention and control, providing references and ideas for the prevention and control of this disease.

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羊地方性鼻内肿瘤是由羊地方性鼻内肿瘤病毒感染引起的一种进行性、接触性传染病，主要表现为羊鼻内筛骨和鼻甲骨上皮黏膜组织出现肿瘤生长。在疾病后期，肿瘤体积显著增大可导致上呼吸道堵塞，进而使发病动物窒息死亡。该病会使感染动物的生产性能大幅下降，给畜牧业带来经济损失，且对珍贵的地方品种和核心种羊群构成威胁，造成优质种羊的损失，成为危害养羊业的重要疫病之一。目前尚无针对该病的疫苗和特效治疗方法。本文就羊地方性鼻内肿瘤病毒的病原学、流行病学、临床症状、主要病理变化、诊断及防治等方面进行综述，为该病的预防和控制提供借鉴与思路。

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Molecular biology detection methods for ENTV

, figureFileSmall=null, figureFileBig=null, tableContent=

检测方法 Methods	靶标 Targets	引物/探针序列 Primer/probe sequences (5′→3′)	参考文献 References
RT-PCR	ENTV-gag	F: GCTGCTTTRAGACCTTATCGAAA R: ATACTGCAGCYCGATGGCCAG	[60]
RT-PCR	ENTV-1-LTR	F: AAGCAAGTTAAGTAACTTGAGATC R: GCTTAGCCGTCCTAAAAGAG	[2]
RT-PCR	ENTV-2-gag	F: AAATGCGACCTTCCGATAATGATGA R: CTTCTGTAGCGGGGACATATT-CTCA	[61]
RT-qPCR (Probe)	ENTV-2-env-U3	F: CCTAACCTTCATTCRTTATGGCARAGT R: CACCGGATCCTTAYGTAATCRGATTTCCTG Probe: FAM-TGTTTAGTTCCTTGCCTCCTTCGTGG-IBFQ	[17]
RT-qPCR (EvaGreen)	ENTV-2-env	F: GAGGCAAATTGAGGCGTTGAT R: CCCGTTCTGCATTCGCTGTAG	[62]
RT-qPCR (Probe)	ENTV-2-env	F: ATGGCAATAGTTTATATCTGCAAT R: GATGGCCTTGTATCAACATAAATGG Probe: FAM-ATATAAGAATCCCGTAACACCTACATCTC-BHQ1	[63]
RT-qPCR (SYBRGreen)	ENTV-2-gag	F: GTCCCTAAAAATGCGACCTT R: GCGACTCCTGAGTTCTGTAAAACCAC	[64]
HRM	ENTV-2-env	F: TCATACTGTGGATTCCCTGTC R: CTAACTACATCATATAAAGCTGATAGTC	[65]
RT-qPCR (Probe)	ENTV-2-env	F: TGGTACGATGAGACTGCCTTAGAG R: CACTTTCGTGACATTATATGACAGG Probe: FAM-CCGCAAGGAAAGAAGTGTGAGCCTGATT-BHQ1	[66]
RT-qPCR (Probe)	ENTV-2-pro	F: GCCTCCTATACAGTACTAGCACCTG R: GATATTAGCTTCCGCTCCTAATAGA Probe: FAM-TGCCTCCAGGGACAGCTGGATTGCTC-BHQ1	[67]

), ArticleFig(id=1238891100279206047, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1238813309860892937, language=CN, label=表1, caption=

ENTV的分子生物学检测方法

, figureFileSmall=null, figureFileBig=null, tableContent=

检测方法 Methods	靶标 Targets	引物/探针序列 Primer/probe sequences (5′→3′)	参考文献 References
RT-PCR	ENTV-gag	F: GCTGCTTTRAGACCTTATCGAAA R: ATACTGCAGCYCGATGGCCAG	[60]
RT-PCR	ENTV-1-LTR	F: AAGCAAGTTAAGTAACTTGAGATC R: GCTTAGCCGTCCTAAAAGAG	[2]
RT-PCR	ENTV-2-gag	F: AAATGCGACCTTCCGATAATGATGA R: CTTCTGTAGCGGGGACATATT-CTCA	[61]
RT-qPCR (Probe)	ENTV-2-env-U3	F: CCTAACCTTCATTCRTTATGGCARAGT R: CACCGGATCCTTAYGTAATCRGATTTCCTG Probe: FAM-TGTTTAGTTCCTTGCCTCCTTCGTGG-IBFQ	[17]
RT-qPCR (EvaGreen)	ENTV-2-env	F: GAGGCAAATTGAGGCGTTGAT R: CCCGTTCTGCATTCGCTGTAG	[62]
RT-qPCR (Probe)	ENTV-2-env	F: ATGGCAATAGTTTATATCTGCAAT R: GATGGCCTTGTATCAACATAAATGG Probe: FAM-ATATAAGAATCCCGTAACACCTACATCTC-BHQ1	[63]
RT-qPCR (SYBRGreen)	ENTV-2-gag	F: GTCCCTAAAAATGCGACCTT R: GCGACTCCTGAGTTCTGTAAAACCAC	[64]
HRM	ENTV-2-env	F: TCATACTGTGGATTCCCTGTC R: CTAACTACATCATATAAAGCTGATAGTC	[65]
RT-qPCR (Probe)	ENTV-2-env	F: TGGTACGATGAGACTGCCTTAGAG R: CACTTTCGTGACATTATATGACAGG Probe: FAM-CCGCAAGGAAAGAAGTGTGAGCCTGATT-BHQ1	[66]
RT-qPCR (Probe)	ENTV-2-pro	F: GCCTCCTATACAGTACTAGCACCTG R: GATATTAGCTTCCGCTCCTAATAGA Probe: FAM-TGCCTCCAGGGACAGCTGGATTGCTC-BHQ1	[67]

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