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Transcription factors are essential for the survival of bacteria, and revealing their functions is conducive to the development of antimicrobials, industrial applications, and environmental protection. At present, there are many methods and strategies for the research on transcription factors, which have been developed rapidly in the fields of bioinformatics and molecular biology. This paper summarizes the methods for identifying unknown transcription factors, unveiling the regulatory functions of transcription factors, constructing regulatory networks of transcription factors, and validating the regulatory genes of transcription factors. This review aims to provide new strategies and new ideas for the analysis and verification of the regulatory functions of bacterial transcription factors.
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转录因子对细菌的生存至关重要，其功能解析有助于推动抗菌药物研发、工业应用以及环境保护等多个领域的发展。目前，转录因子的研究方法与策略种类繁多，在生物信息学和分子生物学等领域均取得了迅速发展。本文从未知转录因子的鉴定方法、转录因子调控功能的鉴定方法、转录因子调控网络的构建方法以及转录因子调控基因的验证方法等4个方面进行综述，为细菌转录因子调控功能的分析与验证提供了新策略与新思路。
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作者贡献声明
陈玲莉：负责论文设计与构思，论文撰写与修改；李晓飞：参与论文讨论，论文修改与校对；刘莹：参与论文讨论，论文修改与校对；任方哲：参与论文讨论，论文修改与校对；焦新安：提供资源；黄金林：获取基金，提供资源，指导论文修改与审阅。
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3.Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou, Jiangsu, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1227681721529856842, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226554097206210577, authorId=1227681721257227060, language=CN, stringName=焦新安, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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3.农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室，江苏 扬州, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1227681717998252663, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226554097206210577, xref=3., ext=[AuthorCompanyExt(id=1227681718006641272, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226554097206210577, companyId=1227681717998252663, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
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1, 2, 3, 4, address=
1.Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China
2.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China
3.Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou, Jiangsu, China
4.Joint International Research Laboratory of Agriculture and Agri-product Safety, Ministry of Education, Yangzhou, Jiangsu, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1227681721940898675, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226554097206210577, authorId=1227681721676657497, language=CN, stringName=黄金林, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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1.扬州大学，江苏省人兽共患病学重点实验室，江苏 扬州
2.江苏高校动物重要疫病与人兽共患病防控协同创新中心，江苏 扬州
3.农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室，江苏 扬州
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