Article(id=1226296959393247294, tenantId=1146029695717560320, journalId=1192105938417971205, issueId=1226296952975966478, articleNumber=null, orderNo=null, doi=10.13343/j.cnki.wsxb.20240592, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1727280000000, receivedDateStr=2024-09-26, revisedDate=null, revisedDateStr=null, acceptedDate=1732118400000, acceptedDateStr=2024-11-21, onlineDate=1770301578614, onlineDateStr=2026-02-05, pubDate=1738598400000, pubDateStr=2025-02-04, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1770301578614, onlineIssueDateStr=2026-02-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1770301578614, creator=13701087609, updateTime=1770301578614, updator=13701087609, issue=Issue{id=1226296952975966478, tenantId=1146029695717560320, journalId=1192105938417971205, year='2025', volume='65', issue='2', pageStart='421', pageEnd='861', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1770301577085, creator=13701087609, updateTime=1770353593135, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1226515124169650204, tenantId=1146029695717560320, journalId=1192105938417971205, issueId=1226296952975966478, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1226515124173844509, tenantId=1146029695717560320, journalId=1192105938417971205, issueId=1226296952975966478, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=758, endPage=770, ext={EN=ArticleExt(id=1226296962341843162, articleId=1226296959393247294, tenantId=1146029695717560320, journalId=1192105938417971205, language=EN, title=Screening and genomic analysis of biocontrol bacteria against cobweb disease of
Oudemansiella raphanipes, columnId=1192149543992045670, journalTitle=Acta Microbiologica Sinica, columnName=Research Article, runingTitle=null, highlight=null, articleAbstract=
Oudemansiella raphanipes is a rare edible and medicinal mushroom, while it is highly susceptible to cobweb disease caused by Cladobotryum varium. [Objective] To obtain bacteria capable of controlling cobweb disease and promoting the mycelial growth of O. raphanipes from casing soil. [Methods] Bacteria were isolated from the casing soil of O. raphanipes, and their antagonistic activity against the mycelial growth of both O. raphanipes and C. varium was evaluated. The strains with disease-preventing and growth-promoting properties were screened out, and their functioning mechanisms were studied based on genome data. [Results] A total of 90 bacterial strains were isolated from the casing soil, of which 38 strains showed significant inhibitory activity against the mycelial growth of C. varium and no inhibitory activity against that of O. raphanipes. The supernatants of two strains significantly inhibited the mycelial growth of C. varium and promoted that of O. raphanipes. Based on the genome data, the above two strains were identified as Peribacillus simplex and Ochrobactrum rhizosphaerae, respectively, and they carried multiple functional genes and biosynthetic gene clusters of secondary metabolites. [Conclusion] Two bacterial strains capable of controlling the disease and promoting the growth of edible fungi were obtained, providing valuable microbial resources for the future biocontrol of cobweb disease.
, correspAuthors=Wentao QIN, authorNote=null, correspAuthorsNote=
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yayong LIU, Taotao ZHANG, Lin GAO, Juan ZHAO, Panpan MENG, Yingfei LI, Wentao QIN), CN=ArticleExt(id=1226296965349159300, articleId=1226296959393247294, tenantId=1146029695717560320, journalId=1192105938417971205, language=CN, title=防治卵孢小奥德蘑蛛网病生防菌筛选及其基因组分析, columnId=1192149544164012138, journalTitle=微生物学报, columnName=研究报告, runingTitle=null, highlight=null, articleAbstract=
卵孢小奥德蘑(Oudemansiella raphanipes)是一种食药兼用的珍稀食用菌,但其易受到异形枝葡霉(Cladobotryum varium)引起的蛛网病侵害。 【目的】 获得能够生物防控蛛网病且促进卵孢小奥德蘑生长的覆土细菌。 【方法】 从卵孢小奥德蘑覆土中分离细菌,检测其对卵孢小奥德蘑及蛛网病菌的拮抗活性,筛选出具有防病促生的多功能菌株,并基于基因组数据分析明确其潜在的生物机制。 【结果】 从覆土中共分离获得90株细菌,其中38株细菌对异形枝葡霉具有明显的抑制活性,且对卵孢小奥德蘑无抑制活性。进一步筛选发现,2株细菌的上清液不仅显著促进卵孢小奥德蘑菌丝的生长,还能显著抑制异形枝葡霉菌丝的生长。基于基因组数据分析,鉴定出上述2株细菌分别为简单近芽孢杆菌(Peribacillus simplex)与根际苍白杆菌(Ochrobactrum rhizosphaerae),且它们含有多种功能基因与次级代谢产物基因簇。 【结论】 本研究获得2株具有食用菌防病促生功能的细菌,为未来蛛网病的生态防控储备了有益微生物资源。
, correspAuthors=秦文韬, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=QYSEvHDEq0V/8gbSgIL0qg==, magXml=yZzOki3Nx3YFYGagcvYOiw==, pdfUrl=null, pdf=4SGj/KgifeMvslTRRgFKpQ==, pdfFileSize=6949815, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=L4bqC/6CJ35mWczApiwojQ==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=I0sMG+goFG9loL28eUH0bw==, mapNumber=null, authorCompany=null, fund=null, authors=
作者贡献声明
刘亚勇:数据收集与处理,论文撰写;张涛涛:微生物分离培养;高琳:微生物分离及鉴定;赵娟:课题讨论,论文修改;孟盼盼:微生物基因组测序;李莹菲:微生物分离培养;秦文韬:课题设计,实验指导,论文修改。
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1, address=1 北京市农林科学院植物保护研究所,农业农村部北方果蔬有害生物绿色防控重点实验室(部省共建),北京, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1226514035986837758, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, xref=null, ext=[AuthorCompanyExt(id=1226514035995226368, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, companyId=1226514035986837758, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-construction by Ministry and Province), Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China), AuthorCompanyExt(id=1226514036003614977, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, companyId=1226514035986837758, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 北京市农林科学院植物保护研究所,农业农村部北方果蔬有害生物绿色防控重点实验室(部省共建),北京)])]), Author(id=1226514036578234685, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1226514036720841031, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, authorId=1226514036578234685, language=EN, stringName=Taotao ZHANG, firstName=Taotao, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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1, address=1 北京市农林科学院植物保护研究所,农业农村部北方果蔬有害生物绿色防控重点实验室(部省共建),北京, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1226514035986837758, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, xref=null, ext=[AuthorCompanyExt(id=1226514035995226368, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, companyId=1226514035986837758, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-construction by Ministry and Province), Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China), AuthorCompanyExt(id=1226514036003614977, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, companyId=1226514035986837758, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 北京市农林科学院植物保护研究所,农业农村部北方果蔬有害生物绿色防控重点实验室(部省共建),北京)])]), Author(id=1226514038583112053, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1226514038708941181, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, authorId=1226514038583112053, language=EN, stringName=Juan ZHAO, firstName=Juan, middleName=null, lastName=ZHAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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1, 2, address=1 Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-construction by Ministry and Province), Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
2 College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1226514039233229217, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, authorId=1226514038956405134, language=CN, stringName=孟盼盼, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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The composition of the isolated bacteria in casing soil of Oudemansiella raphanipes at the genus level., figureFileSmall=Wuchgwc3THbUH82Sxmq56A==, figureFileBig=bX9APO6nLcnUUWsJegLDWA==, tableContent=null), ArticleFig(id=1226514043138126394, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=CN, label=图1, caption=
卵孢小奥德蘑覆土中分离获得的细菌属水平组成, figureFileSmall=Wuchgwc3THbUH82Sxmq56A==, figureFileBig=bX9APO6nLcnUUWsJegLDWA==, tableContent=null), ArticleFig(id=1226514043305898566, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=EN, label=Figure 2, caption=
Inhibitory effect of bacteria in casing soil on the mycelial growth of Oudemansiella raphanipes and Cladobotryum varium. A: Antagonism of Bacillus sp. 11-36 on mycelial growth of O. raphanipes HPJZ; B: Antagonism of Pseudomonas sp. 55-10 on mycelial growth of O. raphanipes HPJZ; C: Antagonism of Chryseobacterium sp.26-1 on mycelial growth of O. raphanipes HPJZ; D: Antagonism of Chryseobacterium sp. 26-1 against mycelial growth of C. varium JZBQA1., figureFileSmall=baWQUcWfZiADl922B/+pZg==, figureFileBig=WXWE9a0QBf5wjaIoRjaD7g==, tableContent=null), ArticleFig(id=1226514043452699213, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=CN, label=图2, caption=
覆土细菌对卵孢小奥德蘑及其蛛网病菌异形枝葡霉的抑制作用。A:芽孢杆菌11-36对卵孢小奥德蘑HPJZ的抑制作用;B:假单胞菌55-10对于卵孢小奥德蘑HPJZ的抑制作用;C:金黄杆菌26-1对卵孢小奥德蘑HPJZ的抑制作用;D:金黄杆菌26-1对异形枝葡霉菌JZBQA1的抑制作用。, figureFileSmall=baWQUcWfZiADl922B/+pZg==, figureFileBig=WXWE9a0QBf5wjaIoRjaD7g==, tableContent=null), ArticleFig(id=1226514043553362515, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=EN, label=Figure 3, caption=
Inhibition of the bacteria in casing soil on the mycelial growth of Oudemansiella raphanipes and Cladobotryum varium., figureFileSmall=8MDG33AbKf+or4uGGLHiVQ==, figureFileBig=9oBy/oPUEsx1HzrNDzug2Q==, tableContent=null), ArticleFig(id=1226514043704357471, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=CN, label=图3, caption=
覆土细菌对卵孢小奥德蘑和异形枝葡霉菌丝生长的抑制作用, figureFileSmall=8MDG33AbKf+or4uGGLHiVQ==, figureFileBig=9oBy/oPUEsx1HzrNDzug2Q==, tableContent=null), ArticleFig(id=1226514043880518243, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=EN, label=Figure 4, caption=
Effect of supernatant of bacteria in casing soil on the mycelial growth of Oudemansiella raphanipes and Cladobotryum varium. A: Effect of supernatant on the mycelial growth of O. raphanipes (*: P<0.05; ***: P<0.001; ****: P<0.000 1); B: Effect of supernatant on the mycelial growth of C. varium (*: P<0.05; **: P<0.01); C: Effect of supernatant of strains 6-22 and 27-12 on the mycelial growth of O. raphanipes and C. varium., figureFileSmall=yCfDDC0O3c4RkTMnhjQ6zg==, figureFileBig=qjqTVO8a9NB62ER2WdLtlw==, tableContent=null), ArticleFig(id=1226514044014735981, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=CN, label=图4, caption=
覆土细菌上清液对卵孢小奥德蘑和异形枝葡霉菌丝生长的影响。A:上清液对卵孢小奥德蘑菌丝生长的影响(*: P<0.05; ***: P<0.001; ****: P<0.000 1);B:上清液对异形枝葡霉菌丝生长的影响(*: P<0.05; **: P<0.01);C:菌株6-22和27-12的上清液对卵孢小奥德蘑和异形枝葡霉菌丝生长的影响。, figureFileSmall=yCfDDC0O3c4RkTMnhjQ6zg==, figureFileBig=qjqTVO8a9NB62ER2WdLtlw==, tableContent=null), ArticleFig(id=1226514044136370801, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=EN, label=Figure 5, caption=
Phylogenetic tree and genome analysis of strain 6-22. A: Colony morphology, Gram staining, and IAA activity detection; B: Phylogenetic tree analysis; C: Secondary metabolite biosynthesis gene clusters; D: Pangenome analysis., figureFileSmall=1HkzJ0IxkeoAdlN0Huy+Eg==, figureFileBig=suMn/Of05fApmVcH4I41YQ==, tableContent=null), ArticleFig(id=1226514044262199932, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=CN, label=图5, caption=
菌株6-22系统发育树及基因组分析。A:菌落形态、革兰氏染色及IAA活性检测;B:系统发育树分析;C:次级代谢产物合成基因簇;D:泛基因组分析。, figureFileSmall=1HkzJ0IxkeoAdlN0Huy+Eg==, figureFileBig=suMn/Of05fApmVcH4I41YQ==, tableContent=null), ArticleFig(id=1226514044383834754, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=EN, label=Figure 6, caption=
Phylogenetic tree and genome analysis of strain 27-12. A: Colony morphology, Gram staining, and IAA activity detection; B: Phylogenetic tree analysis; C: Secondary metabolite biosynthesis gene clusters; D: Pangenome analysis., figureFileSmall=sTEH9O1iJcYmiCFA2UVLRQ==, figureFileBig=oM1WnWhCSrHrSkNXyCj2QA==, tableContent=null), ArticleFig(id=1226514044526441100, tenantId=1146029695717560320, journalId=1192105938417971205, articleId=1226296959393247294, language=CN, label=图6, caption=
菌株27-12系统发育树及基因组分析。A:菌落形态、革兰氏染色及IAA活性检测;B:系统发育树分析;C:次级代谢产物合成基因簇;D:泛基因组分析。, figureFileSmall=sTEH9O1iJcYmiCFA2UVLRQ==, figureFileBig=oM1WnWhCSrHrSkNXyCj2QA==, tableContent=null)], attaches=null, journal=Journal(id=1192105720683257860, delFlag=0, nameCn=微生物学报, nameEn=Acta Microbiologica Sinica, nameHistory1=null, nameHistory2=null, issn=0001-6209, eissn=null, cn=11-1995/Q, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=tNA7JigLZj/rxynSmzKgDQ==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1762149752067, updatedTime=1762150746905, createdBy=18614031015, updatedBy=13701087609, 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