Article(id=1157393073237053622, tenantId=1146029695717560320, journalId=1146032081894723586, issueId=1157393070754025641, articleNumber=null, orderNo=12, doi=10.3981/j.issn.2097-0781.2024.01.011, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1696953600000, receivedDateStr=2023-10-11, revisedDate=1706112000000, revisedDateStr=2024-01-25, acceptedDate=null, acceptedDateStr=null, onlineDate=1753873611982, onlineDateStr=2025-07-30, pubDate=1710864000000, pubDateStr=2024-03-20, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1711468800000, onlineIssueDateStr=2024-03-27, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1753873611982, creator=13701087609, updateTime=1774072494024, updator=sys-migrate, issue=Issue{id=1157393070754025641, tenantId=1146029695717560320, journalId=1146032081894723586, year='2024', volume='3', issue='1', pageStart='100', pageEnd='147', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=1, createTime=1753873611366, creator=13701087609, updateTime=1776075110816, updator=13041195026, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1250512908678017028, tenantId=1146029695717560320, journalId=1146032081894723586, issueId=1157393070754025641, language=EN, specialIssueTitle=Science and Technology Foresight, coverIllustrator=, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1250512908682211333, tenantId=1146029695717560320, journalId=1146032081894723586, issueId=1157393070754025641, language=CN, specialIssueTitle=月球及火星等地外行星科考基地建设探索专刊, coverIllustrator=, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=126, endPage=136, ext={EN=ArticleExt(id=1157393073841033402, articleId=1157393073237053622, tenantId=1146029695717560320, journalId=1146032081894723586, language=EN, title=Development and Suggestions on Key Biotechnology of Extraterrestrial Recyclable Life Support Systems, columnId=1149656489310208610, journalTitle=Science and Technology Foresight, columnName=Review and Commentary, runingTitle=null, highlight=null, articleAbstract=

Building extraterrestrial scientific research bases on the Moon/Mars based on green and recyclable life support systems is an important foundation and prerequisite for deep space exploration. On the basis of an in-depth analysis of the aerospace life support system, the key biotechnology that affects the recyclability of the life support system has been systematically sorted out, and the research status and problems and challenges of material flow control, plant cultivation, biological processing, environmental monitoring and control are summarised, and the development trends are also reviewed. The development of aerospace biocrossover technology driven by metaomics and synthetic biology is proposed, thus providing a reference for the construction of the new generation of life support systems and subsequent extraterrestrial scientific research bases.

, correspAuthors=Xiyu CHENG, authorNote=null, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Qiong YAN, Jinlei ZHANG, Ke FENG, Huanyu LI, Yue LI, Xiyu CHENG), CN=ArticleExt(id=1157393108565676430, articleId=1157393073237053622, tenantId=1146029695717560320, journalId=1146032081894723586, language=CN, title=地外可循环生命保障系统关键生物技术发展与建议, columnId=1148708266483446458, journalTitle=前瞻科技, columnName=综述与述评, runingTitle=null, highlight=null, articleAbstract=

构建以绿色、可循环生命保障系统（简称生保系统）为支撑的月球/火星等地外科考基地，是开展深空探测的重要基础和前提。文章在对航天生保系统进行深入分析的基础上，系统梳理了影响生保系统可循环性能的关键生物技术，总结了物质流调控、植物栽培、生物加工、环境监测与控制技术的研究现状及面临的问题与挑战，并展望了发展趋势。最后提出发展宏基因组学、合成生物学驱动的空天生物交叉技术等对策建议，以期为新一代生保系统与未来地外科考基地建设提供参考。

, correspAuthors=成喜雨, authorNote=null, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=ryPyJGM0Ltvps1aevEpkeQ==, magXml=UYy6NYF14M6TvjcUIT49cg==, pdfUrl=null, pdf=fj/2y8xahsROz9i8t+kGaA==, pdfFileSize=2421028, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=6pQknWuSQQufyfm4Dv6Jbw==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=Lmb7Af9PsBfEimcrng5ftw==, mapNumber=null, authorCompany=null, fund=null, authors=, authorsList=晏琼, 张金磊, 冯可, 李环宇, 李玥, 成喜雨)}, authors=[Author(id=1242113353674457693, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=qyan@bjtu.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1242113353741566559, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, authorId=1242113353674457693, language=EN, stringName=Qiong YAN, firstName=Qiong, middleName=null, lastName=YAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=School of Physical Science and Engineering, Beijing Jiaotong University, Beijing 100044, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1242113353804481120, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, authorId=1242113353674457693, language=CN, stringName=晏琼, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=北京交通大学物理科学与工程学院，北京 100044, bio={"img":"bIZ7kkw/Ye5rneUAl0rbxQ==","content":"

晏琼，副教授。主要从事环境生物技术与生物材料研究。主持和参与军委科技委国防科技创新特区项目、国家自然科学基金、装备预研教育部联合基金、北京市自然科学基金等项目20余项。发表论文30余篇，完成国防科技报告2份等。电子信箱：qyan@bjtu.edu.cn。

"}, bioImg=bIZ7kkw/Ye5rneUAl0rbxQ==, bioContent=

晏琼，副教授。主要从事环境生物技术与生物材料研究。主持和参与军委科技委国防科技创新特区项目、国家自然科学基金、装备预研教育部联合基金、北京市自然科学基金等项目20余项。发表论文30余篇，完成国防科技报告2份等。电子信箱：qyan@bjtu.edu.cn。

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成喜雨，教授，博士研究生导师。主要从事基因与生物技术研究，包括依托系统与合成生物学的仿生抗肿瘤剂型工程、生物保障系统、环境生物技术、生物安全等。主持和参与国家自然科学基金、装备预研教育部联合基金、军委科技委国防科技创新特区项目等项目20余项。发表论文近50篇，授权发明专利5件。电子信箱：xycheng@bjtu.edu.cn。

"}, bioImg=2OMBJI64H6e1dRbogk5WXw==, bioContent=

成喜雨，教授，博士研究生导师。主要从事基因与生物技术研究，包括依托系统与合成生物学的仿生抗肿瘤剂型工程、生物保障系统、环境生物技术、生物安全等。主持和参与国家自然科学基金、装备预研教育部联合基金、军委科技委国防科技创新特区项目等项目20余项。发表论文近50篇，授权发明专利5件。电子信箱：xycheng@bjtu.edu.cn。

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After analyzing the progress in deep space exploration of major aerospace countries and emerging ones in the world, this paper systematically summarizes the main scientific and technological achievements regarding deep space exploration in China. Moreover, it provides an outlook on future deep space exploration and main key technologies. This review can serve as reference for the follow-up development of deep space exploration in China.

), Reference(id=1242113358275609277, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2020, volume=7, issue=3, pageStart=223, pageEnd=231, url=null, language=null, rfNumber=[2], rfOrder=2, authorNames=吴伟仁, 于登云, 王赤, journalName=深空探测学报, refType=null, unstructuredReference=吴伟仁, 于登云, 王赤, 等. 月球极区探测的主要科学与技术问题研究[J]. 深空探测学报, 2020, 7(3): 223-231, 240., articleTitle=月球极区探测的主要科学与技术问题研究, refAbstract=null), Reference(id=1242113358372078271, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.15982/j.issn.2095-7777.2020.20200113001, pmid=null, pmcid=null, year=2020, volume=7, issue=3, pageStart=223, pageEnd=231, url=null, language=null, rfNumber=[2], rfOrder=3, authorNames=Wu W R, Yu D Y, Wang C, journalName=Journal of Deep Space Exploration, refType=null, unstructuredReference=Wu W R, Yu D Y, Wang C, et al. Research on the main scientific and technological issues on lunar polar exploration[J]. Journal of Deep Space Exploration, 2020, 7(3): 223-231, 240. (in Chinese), articleTitle=Research on the main scientific and technological issues on lunar polar exploration, refAbstract=Lunar polar exploration is a strategic point to for all space powers to compete with each other. Based on the brief summary of the development of lunar exploration in domestic and globe，the main scientific and technical issues that need to be solved are studied and analyzed in order to provide a reference for China’s future polar exploration.), Reference(id=1242113358439187137, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2022, volume=52, issue=9, pageStart=1375, pageEnd=1392, url=null, language=null, rfNumber=[3], rfOrder=4, authorNames=刘向阳, 高峰, 邓一兵, journalName=中国科学: 技术科学, refType=null, unstructuredReference=刘向阳, 高峰, 邓一兵, 等. 中国空间站再生生保系统的设计与实现[J]. 中国科学: 技术科学, 2022, 52(9): 1375-1392., articleTitle=中国空间站再生生保系统的设计与实现, refAbstract=null), Reference(id=1242113358518878914, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2022, volume=52, issue=9, pageStart=1375, pageEnd=1392, url=null, language=null, rfNumber=[3], rfOrder=5, authorNames=Liu X Y, Gao F, Deng Y B, journalName=Scientia Sinica (Technologica), refType=null, unstructuredReference=Liu X Y, Gao F, Deng Y B, et al. Design and implementation of regenerative life support system in the China space station[J]. Scientia Sinica (Technologica), 2022, 52(9): 1375-1392. (in Chinese), articleTitle=Design and implementation of regenerative life support system in the China space station, refAbstract=null), Reference(id=1242113359982691012, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=11537780, pmcid=null, year=1984, volume=4, issue=12, pageStart=231, pageEnd=239, url=null, language=null, rfNumber=[4], rfOrder=6, authorNames=Averner M M, Moore B, Bartholomew I, journalName=Advances in Space Research, refType=null, unstructuredReference=Averner M M, Moore B, Bartholomew I, et al. Atmosphere behavior in gas-closed mouse-algal systems: An experimental and modelling study[J]. Advances in Space Research, 1984, 4(12): 231-239., articleTitle=Atmosphere behavior in gas-closed mouse-algal systems: An experimental and modelling study, refAbstract=Concepts of biologically-based regenerative life support systems anticipate the use of photosynthetic organisms for air revitalization. However, mismatches in the rates of production and uptake of oxygen or carbon dioxide between the crew and the plants will lead to an accumulation or depletion of these gases beyond tolerable limits. One method for correcting these atmospheric changes is to use physiochemical devices. This would conflict with the constraint of minimal size and weight imposed upon the successful development of a competitive bioregenerative system. An alternate control strategy is based upon reducing the gas exchange mismatch by manipulation of those environmental parameters known to affect plant or algae gas exchange ratios. We have initiated a research program using a dual approach of mathematical modelling and laboratory experimentation aimed at examining the gas exchange characteristics of artificial animal/plant systems closed to the ambient atmosphere. Our goal is to develop control techniques and management strategies for maintaining the atmospheric levels of carbon dioxide and oxygen at physiological levels. A mathematical model simulating the atmospheric behavior in these systems has been developed and an experimental gas-closed system has been constructed. These will be described and preliminary results will be presented.), Reference(id=1242113360074965702, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.1016/j.lssr.2021.02.005, pmid=33888285, pmcid=null, year=2021, volume=29, issue=null, pageStart=30, pageEnd=37, url=null, language=null, rfNumber=[5], rfOrder=7, authorNames=De Pascale S, Arena C, Aronne G, journalName=Life Sciences in Space Research, refType=null, unstructuredReference=De Pascale S, Arena C, Aronne G, et al. Biology and crop production in space environments: Challenges and opportunities[J]. Life Sciences in Space Research, 2021, 29: 30-37., articleTitle=Biology and crop production in space environments: Challenges and opportunities, refAbstract=Long-term manned space-exploration missions and the permanence of human colonies on orbital stations or planetary habitats will require the regeneration of resources onboard or in-situ. Bioregenerative Life Support Systems (BLSSs) are artificial environments where different compartments, involving both living organisms and physical-chemical processes, are integrated to achieve a safe, self-regulating, and chemically balanced Earth-like environment to support human life. Higher plants are key elements of such systems and Space greenhouses represent the producers' compartment. Growing plants in Space requires the knowledge of their growth responses not only to all environmental factors acting on Earth, but also to specific Space constraints such as altered gravity, ionizing radiations and confined volume. Moreover, cultivation techniques need to be adjusted considering such limitations. The type and intensity of environmental factors to be taken into account depend on the mission scenarios. Here, we summarize constraints and opportunities of cultivating higher plants in Space to regenerate resources and produce fresh food onboard. Both biological and agro-technological issues are considered briefly going through experiments both ground-based on Earth and in Space.Copyright © 2021 The Committee on Space Research (COSPAR). Published by Elsevier B.V. All rights reserved.), Reference(id=1242113360188211912, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.1016/j.lssr.2020.10.002, pmid=33612173, pmcid=null, year=2021, volume=28, issue=null, pageStart=1, pageEnd=10, url=null, language=null, rfNumber=[6], rfOrder=8, authorNames=Brown L, Peick J, Pickett M, journalName=Life Sciences in Space Research, refType=null, unstructuredReference=Brown L, Peick J, Pickett M, et al. Aquatic invertebrate protein sources for long-duration space travel[J]. Life Sciences in Space Research, 2021, 28: 1-10., articleTitle=Aquatic invertebrate protein sources for long-duration space travel, refAbstract=During the summer of 2020, NASA returned to launching astronauts to the International Space Station (ISS) from American soil. By 2024, NASA's mission is to return to the Moon, and by 2028 create a sustainable presence. Long duration missions come with obstacles, especially when trying to create a sustainable environment in a location where "living off the land" is impossible. Some resources on the Moon can be recovered or resupplied; however, many resources such as those needed for sustaining life must be recycled or grown to support humans. To achieve sustainability, food and water must be grown and recycled using elements found within the habitat. NASA's current work focuses on food resupply and growing plants as supplemental nutrient content. This paper examines the possibility for using aquaculture systems to purify water while growing nutrient-rich species as food sources, which aquatic food sources would be ideal for a habitat environment, and which species might provide an ideal test case for future studies aboard ISS. The aquatic species should be rapidly grown with high protein content and low launch mass requirements. Although there are numerous challenges and unknown technology gaps for maintaining aquaculture systems in reduced gravity environments, the benefit of employing such systems would be of great advantage towards creating a sustainable presence beyond Earth's orbit for sustainable aquaculture.Copyright © 2020. Published by Elsevier B.V.), Reference(id=1242113360259515082, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=S2214-5524(18)30033-6, pmid=30797434, pmcid=null, year=2019, volume=20, issue=null, pageStart=53, pageEnd=61, url=null, language=null, rfNumber=[7], rfOrder=9, authorNames=Tikhomirova N A, Trifonov S V, Ushakova S A, journalName=Life Sciences in Space Research, refType=null, unstructuredReference=Tikhomirova N A, Trifonov S V, Ushakova S A, et al. Incorporation of mineralized human waste and fish waste as a source of higher plant mineral nutrition in the BTLSS mass exchange[J]. Life Sciences in Space Research, 2019, 20: 53-61., articleTitle=Incorporation of mineralized human waste and fish waste as a source of higher plant mineral nutrition in the BTLSS mass exchange, refAbstract=The present study deals with the development of the principles and conditions of fish waste mineralization using the method of wet combustion with hydrogen peroxide in alternating electromagnetic field and describes testing mineralized human waste and fish waste as sources of nutrients for plants in the biotechnical human life support system (BTLSS). The study shows that mineralization of fish waste in the wet combustion reactor should be performed in the presence of readily oxidized organic matter, represented by human waste, as an activator of oxidation. Re-mineralization of the sediment in the mixture of hydrogen peroxide and nitric acid in the wet combustion reactor converts mineral elements bound in the sediment into the form available to plants. Using mineralized fish waste as an additional source of mineral elements in the nutrient solutions for growing plants based on mineralized human waste is a way to reduce the amounts of mineral elements added to the solution to replenish it, enabling fuller closure of material loops in the BTLSS.Copyright © 2019 The Committee on Space Research (COSPAR). Published by Elsevier Ltd. All rights reserved.), Reference(id=1242113360330818252, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.1016/j.lssr.2022.03.002, pmid=35491027, pmcid=null, year=2022, volume=33, issue=null, pageStart=33, pageEnd=40, url=null, language=null, rfNumber=[8], rfOrder=10, authorNames=Tikhomirov A, Ushakova S, Velichko V, journalName=Life Sciences in Space Research, refType=null, unstructuredReference=Tikhomirov A, Ushakova S, Velichko V, et al. Possible risks for the functioning of cyclic processes in the experimental model of a closed ecosystem[J]. Life Sciences in Space Research, 2022, 33: 33-40., articleTitle=Possible risks for the functioning of cyclic processes in the experimental model of a closed ecosystem, refAbstract=The purpose of the present study is to consider a number of possible risks that may emerge when processed human wastes are involved into mass exchange processes as fertilizers for plants cultivated in the experimental model of the closed ecosystem (CEEM). The problems relating to the disruption of cycling processes in closed ecosystems can be tentatively divided into two groups: the problems that can be rather easily overcome and the chronic problems. Addition of plant inedible biomass to the soil-like substrate (SLS) can result in a decrease in plant productivity because of allelopathic interactions and enhanced growth of microorganisms. The 30% decrease in wheat productivity by the end of long-duration experiments in the CEEM, with plants grown on quasi-non-renewed solutions based on liquid products prepared by physicochemical mineralization of human wastes, was caused by lower resistance of the plants affected by toxicants accumulated in the solution because of incomplete mineralization of the wastes. The reason for the differences between the macronutrient inflows and outflows was that the donor of human wastes followed a European-type diet while the system produced only part of the plant-based diet. Moreover, macronutrients were partly sorbed in rooting substrates and became unavailable to plants: the substrates in the system retained about 50% of the Ca and 20% ÷ 25% of the Mg, Na, and P inflows over one cycle. These problems are temporary and can be minimized in the foreseeable future.Copyright © 2022. Published by Elsevier B.V.), Reference(id=1242113360402121422, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2014, volume=20, issue=6, pageStart=510, pageEnd=516, url=null, language=null, rfNumber=[9], rfOrder=11, authorNames=艾为党, 郭双生, 董文平, journalName=载人航天, refType=null, unstructuredReference=艾为党, 郭双生, 董文平, 等. “微藻-小白鼠”二元生态系统气体交换规律研究[J]. 载人航天, 2014, 20(6): 510-516., articleTitle=“微藻-小白鼠”二元生态系统气体交换规律研究, refAbstract=null), Reference(id=1242113360473424591, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2014, volume=20, issue=6, pageStart=510, pageEnd=516, url=null, language=null, rfNumber=[9], rfOrder=12, authorNames=Ai W D, Guo S S, Dong W P, journalName=Manned Spaceflight, refType=null, unstructuredReference=Ai W D, Guo S S, Dong W P, et al. Research on gas exchange in the microalgae-mice system[J]. Manned Spaceflight, 2014, 20(6): 510-516. (in Chinese), articleTitle=Research on gas exchange in the microalgae-mice system, refAbstract=null), Reference(id=1242113360565699281, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2016, volume=41, issue=4, pageStart=16, pageEnd=21, url=null, language=null, rfNumber=[10], rfOrder=13, authorNames=杨有泉, 邓素芳, 陈敏, journalName=福建热作科技, refType=null, unstructuredReference=杨有泉, 邓素芳, 陈敏. CELSS系统中两种叶用蔬菜与红萍光合供O₂效应对比研究[J]. 福建热作科技, 2016, 41(4): 16-21., articleTitle=CELSS系统中两种叶用蔬菜与红萍光合供O₂效应对比研究, refAbstract=null), Reference(id=1242113360628613843, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2016, volume=41, issue=4, pageStart=16, pageEnd=21, url=null, language=null, rfNumber=[10], rfOrder=14, authorNames=Yang Y Q, Deng S F, Chen M, journalName=Fujian Hot Work Science and Technology, refType=null, unstructuredReference=Yang Y Q, Deng S F, Chen M. Comparative study on the effect of photosynthetic O₂ feeding by two leafy vegetables and rhizome in CELSS system[J]. Fujian Hot Work Science and Technology, 2016, 41(4): 16-21. (in Chinese), articleTitle=Comparative study on the effect of photosynthetic O₂ feeding by two leafy vegetables and rhizome in CELSS system, refAbstract=null), Reference(id=1242113360687334101, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.1016/j.scitotenv.2018.10.080, pmid=null, pmcid=null, year=2019, volume=651, issue=null, pageStart=2080, pageEnd=2086, url=https://linkinghub.elsevier.com/retrieve/pii/S0048969718339536, language=null, rfNumber=[11], rfOrder=15, authorNames=Zhang L C, Li T, Ai W D, journalName=Science of the Total Environment, refType=null, unstructuredReference=Zhang L C, Li T, Ai W D, et al. Water management in a controlled ecological life support system during a 4-person-180-day integrated experiment: Configuration and performance[J]. Science of the Total Environment, 2019, 651: 2080-2086., articleTitle=Water management in a controlled ecological life support system during a 4-person-180-day integrated experiment: Configuration and performance, refAbstract=null), Reference(id=1242113360746054359, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2022, volume=null, issue=null, pageStart=806, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=16, authorNames=Zhao T, Liu G H, Liu D L, journalName=Science of the Total Environment, refType=null, unstructuredReference=Zhao T, Liu G H, Liu D L, et al. Water recycle system in an artificial closed ecosystem-Lunar Palace 1: Treatment performance and microbial evolution[J]. Science of the Total Environment, 2022, 806, doi: 10.1016/j.scitotenv.2021.151370., articleTitle=Water recycle system in an artificial closed ecosystem-Lunar Palace 1: Treatment performance and microbial evolution, refAbstract=null), Reference(id=1242113360813163225, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2018, volume=31, issue=2, pageStart=273, pageEnd=281, url=null, language=null, rfNumber=[13], rfOrder=17, authorNames=张良长, 李婷, 余青霓, journalName=航天医学与医学工程, refType=null, unstructuredReference=张良长, 李婷, 余青霓, 等. 4人180天集成试验环控生保系统设计及运行概况[J]. 航天医学与医学工程, 2018, 31(2): 273-281., articleTitle=4人180天集成试验环控生保系统设计及运行概况, refAbstract=null), Reference(id=1242113360888660698, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2018, volume=31, issue=2, pageStart=273, pageEnd=281, url=null, language=null, rfNumber=[13], rfOrder=18, authorNames=Zhang L C, Li T, Yu Q N, journalName=Space Medicine & Medical Engineering, refType=null, unstructuredReference=Zhang L C, Li T, Yu Q N, et al. Design and operation overview of 4-person 180-day integrated experiment in controlled ecological life support system[J]. Space Medicine & Medical Engineering, 2018, 31(2): 273-281. (in Chinese), articleTitle=Design and operation overview of 4-person 180-day integrated experiment in controlled ecological life support system, refAbstract=null), Reference(id=1242113360959963868, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.1016/j.lssr.2021.09.003, pmid=34689943, pmcid=null, year=2021, volume=31, issue=null, pageStart=113, pageEnd=120, url=null, language=null, rfNumber=[14], rfOrder=19, authorNames=Liu H, Yao Z K, Fu Y M, journalName=Life Sciences in Space Research, refType=null, unstructuredReference=Liu H, Yao Z K, Fu Y M, et al. Review of research into bioregenerative life support system(s) which can support humans living in space[J]. Life Sciences in Space Research, 2021, 31: 113-120., articleTitle=Review of research into bioregenerative life support system(s) which can support humans living in space, refAbstract=To travel beyond the Earth and realize long-term survival in deep space, humans need to construct Bioregenerative Life Support System (BLSS), which reduces the requirement for supplies from the Earth by in situ regenerating oxygen, water and food needed by astronauts, and prevents pollution to extraterrestrial bodies by recycling waste. Since the 1960s, the USSR/Russia, the United States, Europe, Japan, and China carried out a number of studies with abundant achievements in BLSS systematic theories, plants/animals/microorganisms unit technologies, design/construction, and long-term operation/regulation. China's "Lunar Palace 365″ experiment realized Earth-based closed human survival for a year, with a material closure of >98%. However, a lot of research work is still needed to ultimately realize BLSS application in space, especially given the space experiment of BLSS never carried out, and the overall impact of space environment on BLSS unknown. Lunar exploration projects such as lunar village and lunar research station are successively proceeding. Therefore, future BLSS research will focus on lunar probe payload carrying experiments to study mechanisms of small uncrewed closed ecosystem in space and clarify the impact of space environmental conditions on the ecosystem, so as to correct the design and operation parameters of Earth-based BLSS. Such research will provide theoretical and technological support for BLSS application in crewed deep space exploration.Copyright © 2021. Published by Elsevier B.V.), Reference(id=1242113361048044254, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.1089/ast.2016.1466, pmid=28068148, pmcid=null, year=2017, volume=17, issue=1, pageStart=78, pageEnd=86, url=null, language=null, rfNumber=[15], rfOrder=20, authorNames=Dong C, Fu Y M, Xie B Z, journalName=Astrobiology, refType=null, unstructuredReference=Dong C, Fu Y M, Xie B Z, et al. Element cycling and energy flux responses in ecosystem simulations conducted at the Chinese Lunar Palace-1[J]. Astrobiology, 2017, 17(1): 78-86., articleTitle=Element cycling and energy flux responses in ecosystem simulations conducted at the Chinese Lunar Palace-1, refAbstract=Bioregenerative life-support systems (BLSS) address interactions between organisms and their environment as an integrated system through the study of factors that regulate the pools and fluxes of materials and energy through ecological systems. As a simple model, using BLSS is very important in the investigation of element cycling and energy flux for sustainable development on Earth. A 105-day experiment with a high degree of closure was carried out in this system from February to May, 2014, with three volunteers. The results indicate that 247 g·d carbon was imported into the system from stored food. Most hydrogen is circulated as water, and more than 99% HO can be lost through leaf transpiration into the atmosphere. A total of 1.8 g·d "unknown oxygen" emerged between the input and output of the plant growth module. For the urine processing module, 20.5% nitrogen was reused and 5.35 g·d was put into the nutrient solution. Key Words: Bioregenerative life-support systems (BLSS)-Lunar Palace-1-Artificial ecosystem-Element cycling-Energy flux. 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Since 1980s, space food has been greatly enriched, except the differences in form, most of the foods on earth can be enjoyed in space. In this article, the space foods are classified, in general divided into five parts that include natural form food, intermediate moisture food, thermostabilized food, rehydrating food and irradiated food. New type of space food processing technology is also reviewed, including freeze-drying, irradiation sterilization, high pressure processing, microwave assisted thermal sterilization, food 3 D printing and the packaging of space food products, mainly including the packaging materials already used by the present space food system, and the feasibility analysis of some emerging high barrier packaging materials in the research stage. 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Importantly, the majority of microbes in the environment are difficult to culture under conventional culture conditions. To improve understanding of the microbial community in the space habitat, culture-independent approaches are required. Furthermore, there is a need to assess the bioburden and physiological activity of microbes during future long-term space habitation, so that the "alert" and/or "action" level can be assessed based on real-time changes in the microbial ecosystem. Here, we review the microbial monitoring in the International Space Station-Kibo, and discuss how these results will be adapted to the microbial control in space habitation and pharmaceutical and food processing industries.), Reference(id=1242113364646757144, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2017, volume=null, issue=null, pageStart=7, pageEnd=null, url=null, language=null, rfNumber=[37], rfOrder=50, authorNames=Castro-Wallace S L, Chiu C Y, John K K, journalName=Scientific Reports, refType=null, unstructuredReference=Castro-Wallace S L, Chiu C Y, John K K, et al. Nanopore DNA sequencing and genome assembly on the international space station[J]. 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(in Chinese), articleTitle=Space microbial detection method based on fluorescent LAMP, refAbstract=null), Reference(id=1242113365007467296, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2022, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[40], rfOrder=55, authorNames=姚坤志, journalName=3种盆栽植物对氨气的主动净化, refType=null, unstructuredReference=姚坤志. 3种盆栽植物对氨气的主动净化[D]. 武汉: 华中农业大学, 2022., articleTitle=null, refAbstract=null), Reference(id=1242113365070381857, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2022, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[40], rfOrder=56, authorNames=Yao K Z, journalName=Active removal of ammonia through three potted plants, refType=null, unstructuredReference=Yao K Z. 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(in Chinese), articleTitle=null, refAbstract=null), Reference(id=1242113365133296418, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2013, volume=19, issue=5, pageStart=67, pageEnd=74, url=null, language=null, rfNumber=[41], rfOrder=57, authorNames=郭双生, 董文平, 艾为党, journalName=载人航天, refType=null, unstructuredReference=郭双生, 董文平, 艾为党, 等. 2人30天受控生态生保系统物质流调控技术研究[J]. 载人航天, 2013, 19(5): 67-74., articleTitle=2人30天受控生态生保系统物质流调控技术研究, refAbstract=null), Reference(id=1242113365192016675, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2013, volume=19, issue=5, pageStart=67, pageEnd=74, url=null, language=null, rfNumber=[41], rfOrder=58, authorNames=Guo S S, Dong W P, Ai W D, journalName=Manned Spaceflight, refType=null, unstructuredReference=Guo S S, Dong W P, Ai W D, et al. 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(in Chinese), articleTitle=Design of in situ repair device for the microbial corrosion surface material used in manned spacecraft, refAbstract=null), Reference(id=1242113365510783784, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2017, volume=23, issue=5, pageStart=680, pageEnd=687, url=null, language=null, rfNumber=[44], rfOrder=63, authorNames=郭双生, journalName=载人航天, refType=null, unstructuredReference=郭双生. 我国月球基地受控生态生保系统物质流调控分析研究[J]. 载人航天, 2017, 23(5): 680-687., articleTitle=我国月球基地受控生态生保系统物质流调控分析研究, refAbstract=null), Reference(id=1242113365573698345, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2017, volume=23, issue=5, pageStart=680, pageEnd=687, url=null, language=null, rfNumber=[44], rfOrder=64, authorNames=Guo S S, journalName=Manned Spaceflight, refType=null, unstructuredReference=Guo S S. Analytic study on material flow regulation in CELSS of future Chinese lunar base[J]. Manned Spaceflight, 2017, 23(5): 680-687. (in Chinese), articleTitle=Analytic study on material flow regulation in CELSS of future Chinese lunar base, refAbstract=null), Reference(id=1242113365632418602, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=10.1002/bit.28365, pmid=36859509, pmcid=null, year=2023, volume=120, issue=9, pageStart=2441, pageEnd=2459, url=null, language=null, rfNumber=[45], rfOrder=65, authorNames=Bachhav B, de Rossi J, Llanos C D, journalName=Biotechnology and Bioengineering, refType=null, unstructuredReference=Bachhav B, de Rossi J, Llanos C D, et al. Cell factory engineering: Challenges and opportunities for synthetic biology applications[J]. Biotechnology and Bioengineering, 2023, 120(9): 2441-2459., articleTitle=Cell factory engineering: Challenges and opportunities for synthetic biology applications, refAbstract=The production of high-quality recombinant proteins is critical to maintaining a continuous supply of biopharmaceuticals, such as therapeutic antibodies. Engineering mammalian cell factories presents a number of limitations typically associated with the proteotoxic stress induced upon aberrant accumulation of off-pathway protein folding intermediates, which eventually culminate in the induction of apoptosis. In this review, we will discuss advances in cell engineering and their applications at different hierarchical levels of control of the expression of recombinant proteins, from transcription and translational to posttranslational modifications and subcellular trafficking. We also highlight challenges and unique opportunities to apply modern synthetic biology tools to the design of programmable cell factories for improved biomanufacturing of therapeutic proteins.© 2023 Wiley Periodicals LLC.), Reference(id=1242113365699527467, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2023, volume=4, issue=1, pageStart=1, pageEnd=4, url=null, language=null, rfNumber=[46], rfOrder=66, authorNames=史硕博, 张翀, 成喜雨, journalName=合成生物学, refType=null, unstructuredReference=史硕博, 张翀, 成喜雨, 等. 挑战造物主: 合成生物学使能工具[J]. 合成生物学, 2023, 4(1): 1-4., articleTitle=挑战造物主: 合成生物学使能工具, refAbstract=null), Reference(id=1242113365762442028, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, doi=null, pmid=null, pmcid=null, year=2023, volume=4, issue=1, pageStart=1, pageEnd=4, url=null, language=null, rfNumber=[46], rfOrder=67, authorNames=Shi S B, Zhang C, Cheng X Y, journalName=Synthetic Biology Journal, refType=null, unstructuredReference=Shi S B, Zhang C, Cheng X Y, et al. Challenging the creator: Enabling tools for synthetic biology[J]. Synthetic Biology Journal, 2023, 4(1): 1-4. (in Chinese), articleTitle=Challenging the creator: Enabling tools for synthetic biology, refAbstract=null)], funds=[Fund(id=1242113357898121909, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, awardId=8091B012103, language=CN, fundingSource=装备预研教育部联合基金(8091B012103), fundOrder=null, country=null), Fund(id=1242113357973619383, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, awardId=8091B022138, language=CN, fundingSource=装备预研教育部联合基金(8091B022138), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1242113353598960217, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, xref=null, ext=[AuthorCompanyExt(id=1242113353607348826, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, companyId=1242113353598960217, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=School of Physical Science and Engineering, Beijing Jiaotong University, Beijing 100044, China), AuthorCompanyExt(id=1242113353611543131, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, companyId=1242113353598960217, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=北京交通大学物理科学与工程学院，北京 100044)])], figs=[ArticleFig(id=1242113357210256033, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=EN, label=Fig. 1, caption=Material flow of life support system, figureFileSmall=9nUChPq4TMCIKUIWsolMKg==, figureFileBig=6pQknWuSQQufyfm4Dv6Jbw==, tableContent=null), ArticleFig(id=1242113357268976291, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=CN, label=图1, caption=生保系统物质流示意图, figureFileSmall=9nUChPq4TMCIKUIWsolMKg==, figureFileBig=6pQknWuSQQufyfm4Dv6Jbw==, tableContent=null), ArticleFig(id=1242113357440942761, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=EN, label=Fig. 2, caption=Large-scale ground plant culture device in China, figureFileSmall=JojI9N+4+l5z0HvjnnWboQ==, figureFileBig=kBxzfH/YtPJWa3O30f6Elg==, tableContent=null), ArticleFig(id=1242113357520634539, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=CN, label=图2, caption=中国大型地面植物培养装置, figureFileSmall=JojI9N+4+l5z0HvjnnWboQ==, figureFileBig=kBxzfH/YtPJWa3O30f6Elg==, tableContent=null), ArticleFig(id=1242113357579354797, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=EN, label=Fig. 3, caption=Small space planting devices in various countries, figureFileSmall=4bXrRJqtEJ0bwaWliBNGjg==, figureFileBig=ONc6LUjkO5vTdrpYlDBy+g==, tableContent=null), ArticleFig(id=1242113357642269358, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=CN, label=图3, caption=各国小型空间植物种植装置, figureFileSmall=4bXrRJqtEJ0bwaWliBNGjg==, figureFileBig=ONc6LUjkO5vTdrpYlDBy+g==, tableContent=null), ArticleFig(id=1242113357717766832, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=EN, label=Table 1, caption=

Comparison and analysis of existing life support systems

, figureFileSmall=null, figureFileBig=null, tableContent=

系统	再生保障技术	评价
不可再生生保系统	固废处理：返回后丢弃； 废液处理：返回后丢弃； 气体再生：随飞船携带不可再生	水、气、食物均不可再生。废物不能再利用，只适合短期近地空间探索
物理化学再生生保系统	固废处理：返回后丢弃； 废液处理：过滤、吸附等物化法净化； 气体再生：电解法再生氧气	水、气能够部分再生，但水资源会被逐渐消耗。食物完全不可再生，能满足长时间近地空间活动需要
生物再生生保系统	固废处理：堆肥或焚烧矿化； 废液处理：植物、微生物净化废水； 气体再生：植物、微生物再生净化气体	水、气体、食物能够再生，系统内所有元素都会参与到内部的物质循环，来满足人类长期太空生存需要

), ArticleFig(id=1242113357776487090, tenantId=1146029695717560320, journalId=1146032081894723586, articleId=1157393073237053622, language=CN, label=表1, caption=

现有生保系统的比较和分析

, figureFileSmall=null, figureFileBig=null, tableContent=

系统	再生保障技术	评价
不可再生生保系统	固废处理：返回后丢弃； 废液处理：返回后丢弃； 气体再生：随飞船携带不可再生	水、气、食物均不可再生。废物不能再利用，只适合短期近地空间探索
物理化学再生生保系统	固废处理：返回后丢弃； 废液处理：过滤、吸附等物化法净化； 气体再生：电解法再生氧气	水、气能够部分再生，但水资源会被逐渐消耗。食物完全不可再生，能满足长时间近地空间活动需要
生物再生生保系统	固废处理：堆肥或焚烧矿化； 废液处理：植物、微生物净化废水； 气体再生：植物、微生物再生净化气体	水、气体、食物能够再生，系统内所有元素都会参与到内部的物质循环，来满足人类长期太空生存需要

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