麦类作物学报

小麦TaHsp90基因家族鉴定及其参与耐热的初步研究

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于雪琼 ¹ , 郭志刚 ² , 任众 ² , 崔超 ¹ , 刘湘 ¹ , 姚智翔 ¹ , 王菲 ¹ , 闻珊珊 ¹ , 杨明明 ¹

麦类作物学报 | 遗传育种 2026,46(10): 1290-1302

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麦类作物学报 |遗传育种 2026 , 46 (10) : 1290 -1302

小麦TaHsp90基因家族鉴定及其参与耐热的初步研究

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于雪琼¹, 郭志刚², 任众², 崔超¹, 刘湘¹, 姚智翔¹, 王菲¹, 闻珊珊¹, 杨明明¹

作者信息

^1.西北农林科技大学农学院，陕西杨凌　712100

^2.先正达（中国）投资有限公司，上海　200125

通讯作者:

E-mail：myang@nwafu.edu.cn（杨明明）

作者简介:

E-mail：18536495001@163.com（于雪琼）

Identification of the TaHsp90 Gene Family in Wheat and Its Preliminary Functional Analysis Involved in Thermotolerance

Xueqiong Yu¹, Zhigang Guo², Zhong Ren², Chao Cui¹, Xiang Liu¹, Zhixiang Yao¹, Fei Wang¹, Shanshan Wen¹, Mingming Yang¹

Affiliations

^1.College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China

^2.Syngenta (China) Investment Co., Ltd., Shanghai 200125, China

出版时间: 2026-10-15 doi: 10.7606/j.issn.1009-1041.2026.10.03

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摘要

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热激蛋白Hsp90（heat shock proteins 90）广泛参与植物生长发育进程，同时在植株耐热性调控与高温胁迫响应中具有重要作用。本研究通过生物信息学分析鉴定出18个小麦TaHsp90基因，根据其染色体位置分别命名为TaHsp90-2A～TaHsp90-7D-2，18个TaHsp90基因不均匀分布于小麦9条染色体上。该家族编码蛋白普遍包含10个保守基序。种内共线性分析得到23个共线性基因对。通过系统进化树分析所有Hsp90基因家族可划分为3个亚组。经启动子顺式作用元件分析，TaHsp90基因家族共包含31种顺式作用元件。根据单倍型分析结果，TaHsp90基因家族各成员在单倍型数量、分布频率与地理偏好上差异显著，呈现明显的适应性分化特征。TaHsp90家族在干旱、热胁迫及不同组织中呈现显著特异性表达，可分为4类胁迫响应模式与6类组织表达模式。通过代表基因热胁迫qRT-PCR分析，TaHsp90-2B对热响应更明显。正常条件下，携带TaHsp90-2B株系与其野生型无明显差异；42 ℃热胁迫下，携带TaHsp90-2B株系萎蔫倒伏程度较轻，SPAD值与SOD、POD、CAT活性显著高于野生型，表明该基因可显著增强小麦对热胁迫的耐受性。

关键词

小麦 / Hsp90基因 / 热胁迫 / 表达模式

Abstract

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Heat shock protein 90 (Hsp90) is widely involved in plant growth and development, and plays critical roles in regulating thermotolerance and responses to high-temperature stress in plants. In this study, 18 TaHsp90 genes were identified in wheat via bioinformatic analysis and designated TaHsp90-2A to TaHsp90-7D-2 according to their chromosomal locations. Chromosomal localization revealed that TaHsp90 genes were unevenly distributed across nine wheat chromosomes. Analysis of gene structures and conserved motifs divided this gene family into two structural types, all of which harbored ten conserved motifs. Intraspecific collinearity analysis of wheat identified 23 collinear gene pairs. Phylogenetic tree analysis classified the Hsp90 gene families of all tested species into three subgroups. Promoter cis-acting element detection identified a total of 31 types of cis-elements in the TaHsp90 family. Haplotype analysis indicated that individual TaHsp90 members exhibited significant differences in haplotype number, frequency, and geographical preference, with obvious signatures of adaptive differentiation. Expression profiling revealed distinct tissue-specific and drought-heat-induced expression patterns among TaHsp90 members, which could be categorized into four stress-response modes and six tissue-expression modes. Quantitative real-time PCR (qRT-PCR) of six representative genes with typical stress-responsive patterns demonstrated that TaHsp90-2B exhibited the most prominent heat-inducible expression. Under normal growth conditions, there were no obvious phenotypic differences between carrying TaHsp90-2B wheat lines and wild-type plants. However, after exposure to 42 ℃ heat stress, the overexpression lines exhibited milder wilting and lodging, accompanied by significantly higher SPAD values as well as SOD, POD, and CAT activities compared with the wild type. These results indicate that this gene can markedly improve wheat tolerance to heat stress.

Key words

Wheat / Hsp90 gene / Heat stress / Expression pattern

引用本文

于雪琼, 郭志刚, 任众, 崔超, 刘湘, 姚智翔, 王菲, 闻珊珊, 杨明明. 小麦TaHsp90基因家族鉴定及其参与耐热的初步研究. 麦类作物学报, 2026 , 46 (10) : 1290 -1302 . DOI: 10.7606/j.issn.1009-1041.2026.10.03

Xueqiong Yu, Zhigang Guo, Zhong Ren, Chao Cui, Xiang Liu, Zhixiang Yao, Fei Wang, Shanshan Wen, Mingming Yang. Identification of the TaHsp90 Gene Family in Wheat and Its Preliminary Functional Analysis Involved in Thermotolerance[J]. Journal of Triticeae Crops, 2026 , 46 (10) : 1290 -1302 . DOI: 10.7606/j.issn.1009-1041.2026.10.03

基金

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国家自然科学基金面上项目(32372199)
国家重点研发计划项目(2023YFF1000604)

参考文献

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参考文献引证文献

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2026年第46卷第10期

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doi: 10.7606/j.issn.1009-1041.2026.10.03

接收时间：2026-04-08
首发时间：2026-10-27
出版时间：2026-10-15

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收稿日期：2026-04-08
修回日期：2026-06-27

基金

国家自然科学基金面上项目(32372199)

国家重点研发计划项目(2023YFF1000604)

作者信息

^1.西北农林科技大学农学院，陕西杨凌　712100

^2.先正达（中国）投资有限公司，上海　200125

通讯作者:

E-mail：myang@nwafu.edu.cn（杨明明）

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https://castjournals.cast.org.cn/joweb/mlzwxb/CN/10.7606/j.issn.1009-1041.2026.10.03

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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