Article(id=1307627880012341617, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627835330417093, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.08.05, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1721577600000, receivedDateStr=2024-07-22, revisedDate=1726070400000, revisedDateStr=2024-09-12, acceptedDate=null, acceptedDateStr=null, onlineDate=1789692381146, onlineDateStr=2026-09-18, pubDate=1755187200000, pubDateStr=2025-08-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789692381146, onlineIssueDateStr=2026-09-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789692381146, creator=13701087609, updateTime=1789692381146, updator=13701087609, issue=Issue{id=1307627835330417093, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='8', pageStart='F2', pageEnd='1146', issueExtLink='null', onlineDate='null', pubDate='1755187200000', pubDateStr='2025-08-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789692370493, creator='13701087609', updateTime=1789723574163, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1307758714606346994, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627835330417093, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1307758714610541299, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627835330417093, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1033, endPage=1040, ext={EN=ArticleExt(id=1307627880180113778, articleId=1307627880012341617, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Pathogenic Identification and Evolutionary Analysis of Barley Yellow Dwarf Virus Strainson Wheat in Jiangsu, columnId=1307627250845770215, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breedin, runingTitle=null, highlight=null, articleAbstract=

An investigation on wheat disease in Jiangsu Province was carried out and the samples showed that the tip of the flag leaf expanded to the leaf base and became yellow, and the yellowing accounted for about 1/3 to 1/2 of the whole leaf, which was very similar with the symptoms induced by barley yellow dwarf viruses (BYDV). In order to detect and identify the possible virus associated with the disease, the samples were collected from eight locations in Jiangsu province. A total of 261 samples were detected with various wheat viruses using RT-PCR and 182 samples were found positive when a pair of degenerate primers for BYDV was used. The result of sequence analysis revealed that the virus shared the highest sequence identities with BYDV-PAV. The positive samples were further detected using specific primers for BYDV-PAV coat protein gene (CP) and the results showed that all samples were positive. Further phylogenetic analysis based on the sequence of BYDV-PAV CP gene revealed that the virus detected shared the highest sequence identities with the isolates from Korea. This is the first report of barley yellow dwarf virus-PAV in Wheat grown in Jiangsu province, China.

, authors=Yan DONG1, Shuo LI1, Qian MIAO1, Daoran JIN1, Rongming YANG2, Yinghua JI1, authorsList=Yan DONG, Shuo LI, Qian MIAO, Daoran JIN, Rongming YANG, Yinghua JI, authorCompany=null, correspAuthors=Yinghua JI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307627880716984691, articleId=1307627880012341617, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=江苏地区小麦上大麦黄矮病毒种类鉴定及其进化分析, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

在对江苏小麦病害进行调查时发现疑似感染大麦黄矮病毒(barley yellow dwarf viruses, BYDVs)的病样,病株旗叶由叶尖向叶基扩展变黄,黄化面积占全叶的1/3~1/2。为明确其感染的病毒种类,对田间采集的样品进行检测和鉴定。结果表明,经利用BYDVs通用引物进行检测,采集江苏省8个地点的261份样品中有182份样品扩增到了目的条带。通过序列测定,8个地点样品中的病毒种类均为BYDV-PAV。利用BYDV-PAV外壳蛋白基因(CP)的特异引物对阳性样品进行检测,结果全部为阳性;经对克隆的CP基因序列聚类分析,江苏8个地点的BYDV与韩国的BYDV-PAV株系相对近缘。这是首次发现江苏小麦上的大麦黄矮病毒为PAV株系。

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江苏地区小麦上大麦黄矮病毒种类鉴定及其进化分析
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董岩 1 , 李硕 1 , 缪倩 1 , 靳道然 1 , 杨荣明 2 , 季英华 1
麦类作物学报 | 遗传育种 2025,45(8): 1033-1040
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麦类作物学报 |遗传育种 2025 , 45 (8) : 1033 -1040
江苏地区小麦上大麦黄矮病毒种类鉴定及其进化分析
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董岩1 , 李硕1, 缪倩1, 靳道然1, 杨荣明2, 季英华1
作者信息
  • 1.江苏省农业科学院植物保护研究所,江苏 南京 210014
  • 2.江苏省植物保护植物检疫站,江苏 南京 210014
通讯作者:
E-mail: (季英华)
作者简介:

E-mail: (董岩)

Pathogenic Identification and Evolutionary Analysis of Barley Yellow Dwarf Virus Strainson Wheat in Jiangsu
Yan DONG1 , Shuo LI1, Qian MIAO1, Daoran JIN1, Rongming YANG2, Yinghua JI1
Affiliations
  • 1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
  • 2.Plant Protection and Quarantine Station of Jiangsu Province, Nanjing, Jiangsu 210014, China
出版时间: 2025-08-15 doi: 10.7606/j.issn.1009-1041.2025.08.05
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在对江苏小麦病害进行调查时发现疑似感染大麦黄矮病毒(barley yellow dwarf viruses, BYDVs)的病样,病株旗叶由叶尖向叶基扩展变黄,黄化面积占全叶的1/3~1/2。为明确其感染的病毒种类,对田间采集的样品进行检测和鉴定。结果表明,经利用BYDVs通用引物进行检测,采集江苏省8个地点的261份样品中有182份样品扩增到了目的条带。通过序列测定,8个地点样品中的病毒种类均为BYDV-PAV。利用BYDV-PAV外壳蛋白基因(CP)的特异引物对阳性样品进行检测,结果全部为阳性;经对克隆的CP基因序列聚类分析,江苏8个地点的BYDV与韩国的BYDV-PAV株系相对近缘。这是首次发现江苏小麦上的大麦黄矮病毒为PAV株系。

小麦  /  大麦黄矮病毒PAV株系  /  江苏

An investigation on wheat disease in Jiangsu Province was carried out and the samples showed that the tip of the flag leaf expanded to the leaf base and became yellow, and the yellowing accounted for about 1/3 to 1/2 of the whole leaf, which was very similar with the symptoms induced by barley yellow dwarf viruses (BYDV). In order to detect and identify the possible virus associated with the disease, the samples were collected from eight locations in Jiangsu province. A total of 261 samples were detected with various wheat viruses using RT-PCR and 182 samples were found positive when a pair of degenerate primers for BYDV was used. The result of sequence analysis revealed that the virus shared the highest sequence identities with BYDV-PAV. The positive samples were further detected using specific primers for BYDV-PAV coat protein gene (CP) and the results showed that all samples were positive. Further phylogenetic analysis based on the sequence of BYDV-PAV CP gene revealed that the virus detected shared the highest sequence identities with the isolates from Korea. This is the first report of barley yellow dwarf virus-PAV in Wheat grown in Jiangsu province, China.

Wheat  /  Barley yellow dwarf viruses-PAV  /  Jiangsu
董岩, 李硕, 缪倩, 靳道然, 杨荣明, 季英华. 江苏地区小麦上大麦黄矮病毒种类鉴定及其进化分析. 麦类作物学报, 2025 , 45 (8) : 1033 -1040 . DOI: 10.7606/j.issn.1009-1041.2025.08.05
Yan DONG, Shuo LI, Qian MIAO, Daoran JIN, Rongming YANG, Yinghua JI. Pathogenic Identification and Evolutionary Analysis of Barley Yellow Dwarf Virus Strainson Wheat in Jiangsu[J]. Journal of Triticeae Crops, 2025 , 45 (8) : 1033 -1040 . DOI: 10.7606/j.issn.1009-1041.2025.08.05
  • 国家重点研发计划项目(2023YFD1400300)
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doi: 10.7606/j.issn.1009-1041.2025.08.05
  • 接收时间:2024-07-22
  • 首发时间:2026-09-18
  • 出版时间:2025-08-15
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  • 收稿日期:2024-07-22
  • 修回日期:2024-09-12
基金
国家重点研发计划项目(2023YFD1400300)
作者信息
    1.江苏省农业科学院植物保护研究所,江苏 南京 210014
    2.江苏省植物保护植物检疫站,江苏 南京 210014

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E-mail: (季英华)
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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