Article(id=1307627442336715336, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627419200942839, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.06.01, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1714838400000, receivedDateStr=2024-05-05, revisedDate=1717516800000, revisedDateStr=2024-06-05, acceptedDate=null, acceptedDateStr=null, onlineDate=1789692276796, onlineDateStr=2026-09-18, pubDate=1749916800000, pubDateStr=2025-06-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789692276796, onlineIssueDateStr=2026-09-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789692276796, creator=13701087609, updateTime=1789692276796, updator=13701087609, issue=Issue{id=1307627419200942839, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='6', pageStart='F2', pageEnd='856', issueExtLink='null', onlineDate='null', pubDate='1749916800000', pubDateStr='2025-06-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789692271280, creator='13701087609', updateTime=1789723632162, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1307758957783704401, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627419200942839, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1307758957783704402, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627419200942839, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=711, endPage=718, ext={EN=ArticleExt(id=1307627442550624841, articleId=1307627442336715336, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Analysis on the Function of the Wheat Sugar Transport Protein TaSTP3 Based on the Transcriptome, columnId=1307627250845770215, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breedin, runingTitle=null, highlight=null, articleAbstract=

In order to investigate the sugar transport pathway involved in wheat TaSTP3 gene and its role in wheat-stripe rust interactions, TaSTP3-RNAi transgenic wheat T2 generation plants and Fielder wheat plants were used for RNA-seq, to screen for differentially expressed genes (DEGs) and to use GO and KEGG analyses to speculate on the possible involvement of the TaSTP3 gene in the regulatory pathway and the number of genes involved. The results showed that there were 7 660 DEGs, of which 3 194 were up-regulated and 4 466 were down-regulated. GO functional enrichment revealed that the DEGs were mainly concentrated in the metabolism and synthesis of sugars, carbohydrates, and cellulose; KEGG enrichment analysis showed that the expression of genes in the metabolism and synthesis of sugars, amino acids and lipids changed significantly after TaSTP3 gene silencing, and more genes were enriched in the phytohormone signaling and phenylpropanoid biosynthesis pathways. In summary, it is hypothesized that TaSTP3 alters the distribution of sugars in stripe rust-wheat intercropping by transporting sugars, and in this way transmits molecular signals to affect the metabolism of other nutrients, phytohormone transduction, and photosynthesis, so that target genes interacting with TaSTP3 can be searched from the above pathways, and their involvement in the regulatory pathways can be verified.

, authors=Zhen LIU1, 3, Baoyu HUAI2, 3, Zhensheng KANG2, 3, Jie LIU1, 3, authorsList=Zhen LIU, Baoyu HUAI, Zhensheng KANG, Jie LIU, authorCompany=null, correspAuthors=Jie LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307627442856809034, articleId=1307627442336715336, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=基于转录组分析小麦糖转运蛋白TaSTP3的功能, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

为探究小麦TaSTP3基因参与的糖转运途径及其在小麦与条锈菌互作中的作用,以TaSTP3的RNA沉默转基因小麦T2代植株及其野生型Fielder小麦植株为材料进行转录组(RNA-seq)测序,筛选差异表达基因并进行GO和KEGG富集分析,推测TaSTP3基因可能参与的调控通路和涉及的基因数量。结果表明,有7 660个差异表达基因,其中3 194个上调表达,4 466个下调表达。GO功能富集发现差异表达基因主要集中在糖类、碳水化合物及纤维素的代谢与合成;经KEGG富集分析,TaSTP3基因沉默后糖类、氨基酸和脂质的代谢与合成中的基因表达量发生显著变化,并且还有较多基因富集到植物激素信号转导和苯丙烷类物质生物合成通路。综上所述,推测TaSTP3通过运输糖类物质来改变糖类在条锈菌与小麦互作中的分布情况,以此传递分子信号来影响其他营养物质的代谢、植物激素转导和光合作用。因此,今后可以从上述通路中寻找与TaSTP3互作的靶标基因,并对其参与的调控通路展开验证。

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基于转录组分析小麦糖转运蛋白TaSTP3的功能
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刘榛 1, 3 , 怀宝玉 2, 3 , 康振生 2, 3 , 刘杰 1, 3
麦类作物学报 | 遗传育种 2025,45(6): 711-718
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麦类作物学报 |遗传育种 2025 , 45 (6) : 711 -718
基于转录组分析小麦糖转运蛋白TaSTP3的功能
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刘榛1, 3 , 怀宝玉2, 3, 康振生2, 3, 刘杰1, 3
作者信息
  • 1.西北农林科技大学生命科学学院,陕西 杨凌 712100
  • 2.西北农林科技大学植物保护学院,陕西 杨凌 712100
  • 3.作物抗逆与高效生产全国重点实验室,陕西 杨凌 712100
通讯作者:
E-mail: (刘杰)
作者简介:

E-mail: (刘榛)

Analysis on the Function of the Wheat Sugar Transport Protein TaSTP3 Based on the Transcriptome
Zhen LIU1, 3 , Baoyu HUAI2, 3, Zhensheng KANG2, 3, Jie LIU1, 3
Affiliations
  • 1.College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China
  • 2.College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China
  • 3.State Key Laboratory for Crop Stress Resistance and High Efficiency Production, Yangling, Shaanxi 712100, China
出版时间: 2025-06-15 doi: 10.7606/j.issn.1009-1041.2025.06.01
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为探究小麦TaSTP3基因参与的糖转运途径及其在小麦与条锈菌互作中的作用,以TaSTP3的RNA沉默转基因小麦T2代植株及其野生型Fielder小麦植株为材料进行转录组(RNA-seq)测序,筛选差异表达基因并进行GO和KEGG富集分析,推测TaSTP3基因可能参与的调控通路和涉及的基因数量。结果表明,有7 660个差异表达基因,其中3 194个上调表达,4 466个下调表达。GO功能富集发现差异表达基因主要集中在糖类、碳水化合物及纤维素的代谢与合成;经KEGG富集分析,TaSTP3基因沉默后糖类、氨基酸和脂质的代谢与合成中的基因表达量发生显著变化,并且还有较多基因富集到植物激素信号转导和苯丙烷类物质生物合成通路。综上所述,推测TaSTP3通过运输糖类物质来改变糖类在条锈菌与小麦互作中的分布情况,以此传递分子信号来影响其他营养物质的代谢、植物激素转导和光合作用。因此,今后可以从上述通路中寻找与TaSTP3互作的靶标基因,并对其参与的调控通路展开验证。

小麦  /  TaSTP3  /  RNA干扰  /  转录组

In order to investigate the sugar transport pathway involved in wheat TaSTP3 gene and its role in wheat-stripe rust interactions, TaSTP3-RNAi transgenic wheat T2 generation plants and Fielder wheat plants were used for RNA-seq, to screen for differentially expressed genes (DEGs) and to use GO and KEGG analyses to speculate on the possible involvement of the TaSTP3 gene in the regulatory pathway and the number of genes involved. The results showed that there were 7 660 DEGs, of which 3 194 were up-regulated and 4 466 were down-regulated. GO functional enrichment revealed that the DEGs were mainly concentrated in the metabolism and synthesis of sugars, carbohydrates, and cellulose; KEGG enrichment analysis showed that the expression of genes in the metabolism and synthesis of sugars, amino acids and lipids changed significantly after TaSTP3 gene silencing, and more genes were enriched in the phytohormone signaling and phenylpropanoid biosynthesis pathways. In summary, it is hypothesized that TaSTP3 alters the distribution of sugars in stripe rust-wheat intercropping by transporting sugars, and in this way transmits molecular signals to affect the metabolism of other nutrients, phytohormone transduction, and photosynthesis, so that target genes interacting with TaSTP3 can be searched from the above pathways, and their involvement in the regulatory pathways can be verified.

Wheat  /  TaSTP3  /  RNAi  /  Transcriptome
刘榛, 怀宝玉, 康振生, 刘杰. 基于转录组分析小麦糖转运蛋白TaSTP3的功能. 麦类作物学报, 2025 , 45 (6) : 711 -718 . DOI: 10.7606/j.issn.1009-1041.2025.06.01
Zhen LIU, Baoyu HUAI, Zhensheng KANG, Jie LIU. Analysis on the Function of the Wheat Sugar Transport Protein TaSTP3 Based on the Transcriptome[J]. Journal of Triticeae Crops, 2025 , 45 (6) : 711 -718 . DOI: 10.7606/j.issn.1009-1041.2025.06.01

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doi: 10.7606/j.issn.1009-1041.2025.06.01
  • 接收时间:2024-05-05
  • 首发时间:2026-09-18
  • 出版时间:2025-06-15
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  • 收稿日期:2024-05-05
  • 修回日期:2024-06-05
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    1.西北农林科技大学生命科学学院,陕西 杨凌 712100
    2.西北农林科技大学植物保护学院,陕西 杨凌 712100
    3.作物抗逆与高效生产全国重点实验室,陕西 杨凌 712100

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E-mail: (刘杰)
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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