Article(id=1307627252485747439, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627246831817005, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.12.03, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1741622400000, receivedDateStr=2025-03-11, revisedDate=1753718400000, revisedDateStr=2025-07-29, acceptedDate=null, acceptedDateStr=null, onlineDate=1789692231532, onlineDateStr=2026-09-18, pubDate=1765728000000, pubDateStr=2025-12-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789692231532, onlineIssueDateStr=2026-09-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789692231532, creator=13701087609, updateTime=1789692231532, updator=13701087609, issue=Issue{id=1307627246831817005, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='12', pageStart='F2', pageEnd='1718', issueExtLink='null', onlineDate='null', pubDate='1765728000000', pubDateStr='2025-12-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789692230184, creator='13701087609', updateTime=1789721989753, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1307752068756824724, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627246831817005, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1307752068756824725, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627246831817005, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1606, endPage=1612, ext={EN=ArticleExt(id=1307627252678685424, articleId=1307627252485747439, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Analysis of the Genetic Composition of High-Quality Weak Gluten Wheat Variety Neimai 416 using FISH and 660K Array, columnId=1307627250845770215, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breedin, runingTitle=null, highlight=null, articleAbstract=

To elucidate the chromosomal and molecular genetic composition of the premium weak-gluten wheat variety Neimai 416, fluorescence in situ hybridization (FISH) and 660K wheat SNP array were employed to analyze Neimai 416 and its parental lines She 06-245 and J1094. The results showed that FISH analysis identified 42 chromosomes in the root tip cells of Neimai 416, with stable inheritance. The Oligo-pSc119.2 FISH signals on chromosomes 4A, 5A, 1B, and 6B differed from those of the maternal parent She 06-245, and except for 1B, also differed from the paternal parent J1094. The 660K SNP array results revealed that the maternal parent She 06-245 contributed 66.51% of the genetic material to Neimai 416, while the paternal parent J1094 contributed 33.49%, indicating a genetic bias towards the maternal parent in Neimai 416. At the genome level, the maternal parent She 06-245 exhibited higher genetic contributions to the A, B, and D genomes (73.84%, 60.73%, and 61.64%, respectively) compared to the paternal parent J1094. Chromosomally, except for chromosomes 1B, 6B, 4D, and 7D, She 06-245 showed greater genetic contributions than J1094 across all other chromosomes, with particularly great contributions on 7A (91.98%) and 3D (90.32%). Functional marker analysis revealed that Neimai 416 integrates multiple genes of high thousand-kernel weight (TaGS2B1, TaGW2-6B, TaGS-D1, TaSus2-2A, TaCwi, and TaGS2A1), along with superior alleles associated with disease resistance (COMT3B, 1-FEH-6B, TaDREB1, Vp1B1, and Lr34) and agronomic traits (Rht-D1b and TamybR_B1b). This study demonstrates that Neimai 416 exhibits chromosomal stability, a pronounced maternal-biased inheritance pattern, and carries multiple high thousand-kernel weight genes and resistance-related superior genes.

, authors=Shuxian GUAN, Huiyue HUANG, Renquan WANG, Xiangquan WANG, Jiezhi YANG, Yanxia SONG, Liping HUANG, Min YANG, authorsList=Shuxian GUAN, Huiyue HUANG, Renquan WANG, Xiangquan WANG, Jiezhi YANG, Yanxia SONG, Liping HUANG, Min YANG, authorCompany=null, correspAuthors=Huiyue HUANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307627253022618353, articleId=1307627252485747439, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=基于FISH和660K芯片的优质弱筋小麦品种内麦416的遗传构成解析, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

为探明优质弱筋小麦品种内麦416的染色体和分子遗传构成,利用荧光原位杂交(FISH)和660K芯片对内麦416及其双亲射06-245和J1094进行分析。结果表明,内麦416根尖细胞含有42条染色体,遗传稳定,4A、5A、1B和6B染色体的Oligo-pSc119.2 FISH信号与母本射06-245有差异,除1B外,与亲本J1094也有差异。通过660K芯片结果显示,母本射06-245对内麦416的遗传贡献率为66.51%,父本J1094的遗传贡献率为33.49%,说明内麦416具有遗传偏亲现象;在基因组水平,母本射06-245对A、B和D 3个基因组的遗传贡献率(分别为73.84%、60.73%和61.64%)高于父本J1094;在染色体水平,除1B、6B、4D和7D外,其余染色体的遗传贡献率均表现为射06-245高于J1094,其中7A、3D的遗传贡献率分别高达91.98%和90.32%。经功能标记鉴定,内麦416包含6个高千粒重基因(TaGS2B1、TaGW2-6B、TaGS-D1、TaSus2-2A、TaCwi、TaGS2A1),还携带有与抗性(COMT3B、1-FEH-6B、TaDREB1、Vp1B1、Lr34)和农艺性状(Rht-D1b、TamybR_B1b)等相关的优异基因。由此可见,内麦416染色体具有遗传稳定性,表现出明显的母本遗传偏亲特征,且携带多个高千粒重基因及与抗性相关的优良基因。

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基于FISH和660K芯片的优质弱筋小麦品种内麦416的遗传构成解析
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关淑仙 , 黄辉跃 , 汪仁全 , 王相权 , 杨杰智 , 宋艳霞 , 黄丽萍 , 杨敏
麦类作物学报 | 遗传育种 2025,45(12): 1606-1612
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麦类作物学报 |遗传育种 2025 , 45 (12) : 1606 -1612
基于FISH和660K芯片的优质弱筋小麦品种内麦416的遗传构成解析
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E-mail: (关淑仙)

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关淑仙 , 黄辉跃 , 汪仁全, 王相权, 杨杰智, 宋艳霞, 黄丽萍, 杨敏
作者信息
  • 四川省内江市农业科学院,四川 内江 641000
通讯作者:
E-mail: (黄辉跃)
作者简介:

E-mail: (关淑仙)

Analysis of the Genetic Composition of High-Quality Weak Gluten Wheat Variety Neimai 416 using FISH and 660K Array
Shuxian GUAN , Huiyue HUANG , Renquan WANG, Xiangquan WANG, Jiezhi YANG, Yanxia SONG, Liping HUANG, Min YANG
Affiliations
  • Neijiang Academy of Agricultural Sciences, Neijiang, Sichuan 641000, China
出版时间: 2025-12-15 doi: 10.7606/j.issn.1009-1041.2025.12.03
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为探明优质弱筋小麦品种内麦416的染色体和分子遗传构成,利用荧光原位杂交(FISH)和660K芯片对内麦416及其双亲射06-245和J1094进行分析。结果表明,内麦416根尖细胞含有42条染色体,遗传稳定,4A、5A、1B和6B染色体的Oligo-pSc119.2 FISH信号与母本射06-245有差异,除1B外,与亲本J1094也有差异。通过660K芯片结果显示,母本射06-245对内麦416的遗传贡献率为66.51%,父本J1094的遗传贡献率为33.49%,说明内麦416具有遗传偏亲现象;在基因组水平,母本射06-245对A、B和D 3个基因组的遗传贡献率(分别为73.84%、60.73%和61.64%)高于父本J1094;在染色体水平,除1B、6B、4D和7D外,其余染色体的遗传贡献率均表现为射06-245高于J1094,其中7A、3D的遗传贡献率分别高达91.98%和90.32%。经功能标记鉴定,内麦416包含6个高千粒重基因(TaGS2B1、TaGW2-6B、TaGS-D1、TaSus2-2A、TaCwi、TaGS2A1),还携带有与抗性(COMT3B、1-FEH-6B、TaDREB1、Vp1B1、Lr34)和农艺性状(Rht-D1b、TamybR_B1b)等相关的优异基因。由此可见,内麦416染色体具有遗传稳定性,表现出明显的母本遗传偏亲特征,且携带多个高千粒重基因及与抗性相关的优良基因。

内麦416  /  FISH  /  660K芯片  /  遗传构成

To elucidate the chromosomal and molecular genetic composition of the premium weak-gluten wheat variety Neimai 416, fluorescence in situ hybridization (FISH) and 660K wheat SNP array were employed to analyze Neimai 416 and its parental lines She 06-245 and J1094. The results showed that FISH analysis identified 42 chromosomes in the root tip cells of Neimai 416, with stable inheritance. The Oligo-pSc119.2 FISH signals on chromosomes 4A, 5A, 1B, and 6B differed from those of the maternal parent She 06-245, and except for 1B, also differed from the paternal parent J1094. The 660K SNP array results revealed that the maternal parent She 06-245 contributed 66.51% of the genetic material to Neimai 416, while the paternal parent J1094 contributed 33.49%, indicating a genetic bias towards the maternal parent in Neimai 416. At the genome level, the maternal parent She 06-245 exhibited higher genetic contributions to the A, B, and D genomes (73.84%, 60.73%, and 61.64%, respectively) compared to the paternal parent J1094. Chromosomally, except for chromosomes 1B, 6B, 4D, and 7D, She 06-245 showed greater genetic contributions than J1094 across all other chromosomes, with particularly great contributions on 7A (91.98%) and 3D (90.32%). Functional marker analysis revealed that Neimai 416 integrates multiple genes of high thousand-kernel weight (TaGS2B1, TaGW2-6B, TaGS-D1, TaSus2-2A, TaCwi, and TaGS2A1), along with superior alleles associated with disease resistance (COMT3B, 1-FEH-6B, TaDREB1, Vp1B1, and Lr34) and agronomic traits (Rht-D1b and TamybR_B1b). This study demonstrates that Neimai 416 exhibits chromosomal stability, a pronounced maternal-biased inheritance pattern, and carries multiple high thousand-kernel weight genes and resistance-related superior genes.

Neimai 416  /  Fluorescence in situ hybridization  /  660K array  /  Genetic composition
关淑仙, 黄辉跃, 汪仁全, 王相权, 杨杰智, 宋艳霞, 黄丽萍, 杨敏. 基于FISH和660K芯片的优质弱筋小麦品种内麦416的遗传构成解析. 麦类作物学报, 2025 , 45 (12) : 1606 -1612 . DOI: 10.7606/j.issn.1009-1041.2025.12.03
Shuxian GUAN, Huiyue HUANG, Renquan WANG, Xiangquan WANG, Jiezhi YANG, Yanxia SONG, Liping HUANG, Min YANG. Analysis of the Genetic Composition of High-Quality Weak Gluten Wheat Variety Neimai 416 using FISH and 660K Array[J]. Journal of Triticeae Crops, 2025 , 45 (12) : 1606 -1612 . DOI: 10.7606/j.issn.1009-1041.2025.12.03
  • 四川省“十四五”生物育种重大科技专项(2022ZDZX0014)
  • 四川省“十四五”农作物及畜禽育种攻关项目(2021YFYZ0002-5)
  • 国家现代农业产业技术体系四川麦类作物创新团队项目(SCCXTD-2024-11)
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doi: 10.7606/j.issn.1009-1041.2025.12.03
  • 接收时间:2025-03-11
  • 首发时间:2026-09-18
  • 出版时间:2025-12-15
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  • 收稿日期:2025-03-11
  • 修回日期:2025-07-29
基金
四川省“十四五”生物育种重大科技专项(2022ZDZX0014)
四川省“十四五”农作物及畜禽育种攻关项目(2021YFYZ0002-5)
国家现代农业产业技术体系四川麦类作物创新团队项目(SCCXTD-2024-11)
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    四川省内江市农业科学院,四川 内江 641000

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E-mail: (黄辉跃)
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https://castjournals.cast.org.cn/joweb/mlzwxb/CN/10.7606/j.issn.1009-1041.2025.12.03
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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