Article(id=1305139526662377877, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305139508656230632, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2026.02.05, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1750608000000, receivedDateStr=2025-06-23, revisedDate=1760976000000, revisedDateStr=2025-10-21, acceptedDate=null, acceptedDateStr=null, onlineDate=1789099111475, onlineDateStr=2026-09-11, pubDate=1771084800000, pubDateStr=2026-02-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789099111475, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789099111475, creator=13701087609, updateTime=1789099111475, updator=13701087609, issue=Issue{id=1305139508656230632, tenantId=1146029695717560320, journalId=1303687221787852879, year='2026', volume='46', issue='2', pageStart='F2', pageEnd='278', issueExtLink='null', onlineDate='null', pubDate='1771084800000', pubDateStr='2026-02-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789099107183, creator='13701087609', updateTime=1789103172485, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305156559844176362, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305139508656230632, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305156559844176363, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305139508656230632, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=187, endPage=198, ext={EN=ArticleExt(id=1305139530328199574, articleId=1305139526662377877, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Identification of Powdery Mildew Resistance Genes in Wheat, columnId=1305133119129023428, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici, is a devastating foliar disease that significantly threatens wheat production. In this study, molecular marker and Sanger sequencing were adopted to analyse the composition or sequence variation of 17 cloned or common powdery mildew (Pm) resistance genes in 40 wheat varieties mainly from Yuncheng city. Molecular markers were used to identify the 15 Pm loci, and the results showed that five loci (Pm2, Pm6, Pm8, Pm30 and Pm52) were identified as single gene or gene pyramiding. The frequency of Pm2, Pm6, Pm8, and Pm30 in the tested varieties was 35%, 20%, 20%, and 10%, respectively. Five varieties (Yunmai 2008, Jimai 22, Zhongmai 30, GA20015, and Liangxing 99) have both Pm2 and Pm6, and combination of Pm8 and Pm30 was only detected in Yunhei 1619 and Jinzimai 33. To analyse the variation of partial resistance gene Pm46, a pair of specific primers was designed to amplify the full-length genomic sequence of Pm46 in the 40 varieties. A predicted 4 708 bp DNA fragment was obtained in 34 varieties whereas no amplicon was obtained from the remaining six colored wheat varieties. By marker analysis, the six varieties all carry 4Ag (4E) chromosome of blue-grained wheat, which is the donor of ThMYC4E. Pm46 gene was sequenced in the 34 varieties and 3 haplotypes were identified, all of which encode susceptible proteins. Additionally, allelic variation analysis of Pm5 gene in the tested materials revealed that only Linnuo 178 carries the resistant allele Pm5b, while the resistant allele Pm5e was not detected. The phenotypic identification results show that germplasm carrying the Pm6 and germplasm carrying the blue-grained wheat 4Ag chromosome exhibit good resistance to the local powdery mildew races in Yuncheng, which can be further used for future wheat genetic improvement.

, authors=Feng LI, Yue CAI, Zhiyong ZHAO, Wenbin REN, Jianchun FAN, Anhong ZHANG, authorsList=Feng LI, Yue CAI, Zhiyong ZHAO, Wenbin REN, Jianchun FAN, Anhong ZHANG, authorCompany=null, correspAuthors=Anhong ZHANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305139530533720471, articleId=1305139526662377877, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=小麦品种（系）抗白粉病基因的鉴定, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

小麦白粉病是严重危害小麦生产的重要病害。本研究采用分子标记和基因测序技术对40份小麦品种（系）共17个已克隆或常用抗白粉病基因的组成及序列变异进行了分析。供试材料15个基因位点（Pm1、Pm2、Pm3、Pm4、Pm6、Pm8、Pm12、Pm17、Pm21、Pm24、Pm30、Pm38、Pm52、Pm60、Pm69）的标记检测结果显示，在5个位点上检测到已知抗白粉病基因Pm2、Pm6、Pm8、Pm30和Pm52，以单基因或基因聚合形式存在，其中Pm2、Pm6、Pm8、Pm30的阳性率分别达35%、20%、20%和10%。同时携带Pm2和Pm6的材料有5个，分别为运麦2008、济麦22、中麦30、GA20015和良星99；同时携带Pm8和Pm30的材料是运黑1619和晋紫麦33。使用基因全长特异引物对40份小麦材料中的广谱抗病基因Pm46进行高保真扩增后发现，有34份材料能够扩增出稳定单一的目标条带（4 708 bp），其余6个无扩增产物的材料均为特色小麦。经分子标记检测，这6份材料均携带4Ag（4E）染色体，是蓝粒基因Ba1（ThMYC4E）的供体。通过对34份材料的Pm46基因进行基因测序及单倍型分析，共检测到3种单倍型，均为感病等位基因。利用测序和分子标记辅助选择对供试材料中的Pm5基因进行了等位变异分析，只有临糯178携带Pm5的抗病等位基因Pm5b，未检测到抗病等位基因Pm5e。经表型鉴定，携带提莫菲维小麦Pm6和携带蓝粒小麦4Ag染色体的种质对运城本地白粉菌生理小种表现出较好的抗病性，能够进一步用于未来的小麦遗传改良。

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