Article(id=1305139518068248896, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305139508656230632, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2026.02.01, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1741708800000, receivedDateStr=2025-03-12, revisedDate=1743523200000, revisedDateStr=2025-04-02, acceptedDate=null, acceptedDateStr=null, onlineDate=1789099109427, onlineDateStr=2026-09-11, pubDate=1771084800000, pubDateStr=2026-02-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789099109427, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789099109427, creator=13701087609, updateTime=1789099109427, updator=13701087609, issue=Issue{id=1305139508656230632, tenantId=1146029695717560320, journalId=1303687221787852879, year='2026', volume='46', issue='2', pageStart='F2', pageEnd='278', issueExtLink='null', onlineDate='null', pubDate='1771084800000', pubDateStr='2026-02-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789099107183, creator='13701087609', updateTime=1789103172485, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305156559844176362, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305139508656230632, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305156559844176363, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305139508656230632, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=141, endPage=153, ext={EN=ArticleExt(id=1305139519825662273, articleId=1305139518068248896, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Construction of Expression Profiling during Grain Filling Stage and Analysis of Grain Color-Related Genes in Blue-Grained Wheat, columnId=1305133119129023428, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

The aleurone layer of blue-grained wheat is rich in anthocyanins, which offer numerous health benefits to humans. To elucidate the molecular mechanisms underlying anthocyanin synthesis and regulation in the aleurone layer, this study compared the blue-grained wheat material E10-2B with its white-grained counterpart E10-2W using agronomic trait evaluation, molecular markers, liquid array technology, and transcriptome analysis. The results showed that compared to E10-2W, E10-2B exhibited a 15.31% reduction in plant height, indicating a dwarfing trait. However, its panicle length, 1 000-grain weight, and grain length decreased by 17.93%, 5.99%, and 4.40%, respectively. Molecular marker and liquid chip analyses revealed that E10-2B contains the complete genome of common wheat, along with the 4E chromosome from Thinopyrum ponticum. The grain color of E10-2B undergoes a transformation between 20 and 25 days post-anthesis, with 25 days post-anthesis identified as the critical time point for anthocyanin accumulation. Transcriptome sequencing analysis identified 748 and 5 246 differentially expressed genes at 20 and 25 days post-flowering, respectively. KEGG enrichment analysis indicated that the differentially expressed genes at 25 days post-flowering were significantly enriched in pathways related to phenylpropanoid, flavonoid, and anthocyanin biosynthesis, suggesting activation of the anthocyanin synthesis pathway at this stage. To further identify key genes in the anthocyanin synthesis pathway, we analyzed transcriptome data from the blue-grained wheat material Blue 1 and its white-grained control White 1, available in public databases. Comparative analysis focused on the expression patterns of anthocyanin synthesis-related genes in the two blue-grained materials (E10-2B and Blue 1). A total of 77 and 21 up-regulated anthocyanin synthesis-related genes were identified in E10-2B and Blue 1, respectively, including chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and MYB transcription factors. Notably, 15 genes showed shared up-regulation in both blue-grained materials, accounting for 19.48% of the up-regulated genes in E10-2B. Among these, three flavonoid 3′,5′-hydroxylase genes (F3′5′H) and three flavonoid 3-O-glucosyltransferase genes (UFGT) exhibited high expression levels in both materials. These co-upregulated genes likely play a pivotal role in the differential anthocyanin accumulation between blue and white wheat.

, authors=Zuchun LI, Chenxi HUANG, Jingli FAN, Hao WANG, Jianing ZHAO, Yue GUAN, Xiaoqi ZHAO, Changyou WANG, Xinlun LIU, Jixin ZHAO, Tingdong LI, Chunhuan CHEN, Pingchuan DENG, Wanquan JI, authorsList=Zuchun LI, Chenxi HUANG, Jingli FAN, Hao WANG, Jianing ZHAO, Yue GUAN, Xiaoqi ZHAO, Changyou WANG, Xinlun LIU, Jixin ZHAO, Tingdong LI, Chunhuan CHEN, Pingchuan DENG, Wanquan JI, authorCompany=null, correspAuthors=Wanquan JI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305139520031183170, articleId=1305139518068248896, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=蓝粒小麦籽粒灌浆期表达谱构建及粒色相关基因分析, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

蓝粒小麦的籽粒糊粉层中富含大量的花青素，对人类健康具有多种益处。为了解析其糊粉层中花青素的合成与调控的分子机制，利用农艺性状评估、分子标记、液相芯片技术及转录组分析等方法对蓝粒材料E10-2B与白粒材料E10-2W进行了比较。结果表明，相较于E10-2W，E10-2B的株高、穗长、千粒重和粒长分别降低了15.31%、17.93%、5.99%和4.40%。从分子标记和液相芯片分析结果看，E10-2B不仅含有完整的普通小麦染色体组，还附加了十倍体长穗偃麦草的4E染色体。E10-2B籽粒颜色在花后20~25 d间发生转变，花后25 d是花青素积累的关键时间点。通过转录组测序分析，花后20和25 d在E10-2B中分别出现了748和5 246个差异基因。经KEGG富集分析，花后25 d的差异基因富集到苯丙烷、类黄酮、花青素生物合成途径等通路，花青素合成通路此时被激活。通过参考公共数据库中蓝粒小麦材料Blue 1及其白粒对照White 1的转录组数据，两个蓝粒材料（B10-2B和Blue 1）中分别有77和21个花青素合成相关基因上调表达，涉及查尔酮合酶（CHS）、黄烷酮3-羟化酶（F3H）、MYB转录因子等。值得注意的是，两个蓝粒材料中有15个基因共同上调表达，占B10-2B上调基因的19.48%，其中3个F3′5′H基因（类黄酮3′，5′-羟化酶基因）和3个UFGT基因（类黄酮3-O-葡糖基转移酶基因）在两个蓝粒材料中均表现出高表达。这些共同上调的基因可能在蓝粒与白粒小麦的花青素积累差异中起关键作用。

, authors=李祖春, 黄晨曦, 范京蠡, 王浩, 赵嘉宁, 管悦, 赵肖琦, 王长有, 刘新伦, 赵继新, 李停栋, 陈春环, 邓平川, 吉万全, authorsList=李祖春, 黄晨曦, 范京蠡, 王浩, 赵嘉宁, 管悦, 赵肖琦, 王长有, 刘新伦, 赵继新, 李停栋, 陈春环, 邓平川, 吉万全, authorCompany=null, correspAuthors=吉万全, authorNote=, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=OkaGCMVtI7EltO+Nl0zPtw==, magXml=afgOv7/31QMUOf8+4HUyeg==, pdfUrl=null, pdf=L4cQDK2JyTsSqzVn9+nuGQ==, pdfFileSize=6516150, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=lGQYq/yyhprT3KaljlWofQ==, mapNumber=null, fund=null)}, authors=[Author(id=1305139520333173063, tenantId=1146029695717560320, journalId=1303687221787852879, articleId=1305139518068248896, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=2022050128@nwafu.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1305139520412864841, tenantId=1146029695717560320, journalId=1303687221787852879, articleId=1305139518068248896, authorId=1305139520333173063, language=EN, stringName=Zuchun LI, firstName=Zuchun, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1305139520563859786, tenantId=1146029695717560320, journalId=1303687221787852879, articleId=1305139518068248896, authorId=1305139520333173063, language=CN, stringName=李祖春, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=西北农林科技大学农学院，陕西 杨凌 712100, bio={"content":"

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E-mail: 2022050128@nwafu.edu.cn（李祖春）

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