Article(id=1305136397803876956, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136265016398371, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.01.05, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1718208000000, receivedDateStr=2024-06-13, revisedDate=1726588800000, revisedDateStr=2024-09-18, acceptedDate=null, acceptedDateStr=null, onlineDate=1789098365497, onlineDateStr=2026-09-11, pubDate=1736870400000, pubDateStr=2025-01-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789098365497, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789098365497, creator=13701087609, updateTime=1789098365497, updator=13701087609, issue=Issue{id=1305136265016398371, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='1', pageStart='1', pageEnd='140', issueExtLink='null', onlineDate='null', pubDate='1736870400000', pubDateStr='2025-01-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789098333839, creator='13701087609', updateTime=1789103362193, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305157355486859379, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136265016398371, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305157355491053684, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136265016398371, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=45, endPage=51, ext={EN=ArticleExt(id=1305136398005203549, articleId=1305136397803876956, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Evaluation of Molecular Markers for Wheat Stripe Rust Resistance Genes Yr5, Yr9 and Yr18, columnId=1305133119129023428, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

The traits of Yr5, Yr9 (1B/1R), and Yr18 genes play an important role in wheat breeding in China, and the molecular markers STS9/10 of Yr5, AF1/4, D15, 20H of Yr9 (1B/1R) and csLV34, cssfr1~cssfr5 of Yr18 were effectively tested by applying near isogenic lines under background of Avocet S and lines with known Yr genes. The results showed that the specific molecular markers of Yr5 gene STS9/10, Yr9 (1B/1R) gene AF1/4, D15 and 20H could accurately identify the corresponding resistance genes in different genetic background materials. Yr18 gene molecular marker cssfr2 could accurately detect the materials not-carrying Yr18, and detect allelic variation of Yr18 in Avocet S*6/Yr5, Avocet S*6/Yr24, and Avocet S*6/Yr27. There fore, the linkage marker STS9/10 of Yr5 gene, the molecular markers AF1/4, D15 and 20H of Yr9 (1B/1R) gene can effectively detect the target gene, and the combination of several molecular markers of Yr18 gene not only effectively detected the target gene but also identified the allelic variation of this site.

, authors=Zhenyu SUN1, Liang HUANG2, Miaomiao HUANG3, Taiguo LIU2, authorsList=Zhenyu SUN, Liang HUANG, Miaomiao HUANG, Taiguo LIU, authorCompany=null, correspAuthors=Taiguo LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305136398311387742, articleId=1305136397803876956, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=小麦抗条锈基因Yr5Yr9Yr18分子标记的特异性评估, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

Yr5Yr9(1B/1R)、Yr18基因的自身性状或连锁性状在中国小麦育种中具有重要的应用前景。本研究运用以Avocet S为背景的单基因近等基因系及其对应基因的载体品种,对Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记AF1/4、D15、20H,Yr18基因的分子标记csLV34、cssfr1~cssfr5进行有效性验证。结果表明,Yr5基因的特异性分子标记STS9/10、Yr9(1B/1R)基因的特异性分子标记AF1/4、D15和20H均能够准确识别不同遗传背景材料中的相应抗性基因;Yr18基因分子标记cssfr2能够准确检测Yr18的非载体材料,并检测出Avocet S*6/Yr5、Avocet S*6/Yr24、Avocet S*6/Yr27三个材料具有Yr18的等位变异。这说明Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记AF1/4、D15、20H能对目的基因进行有效检测,Yr18基因的几个分子标记结合使用不仅能够对目的基因进行有效检测,而且能够识别该位点的等位变异。

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E-mail: (孙振宇)

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小麦抗条锈基因Yr5Yr9Yr18分子标记的特异性评估
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孙振宇 1 , 黄亮 2 , 黄苗苗 3 , 刘太国 2
麦类作物学报 | 遗传育种 2025,45(1): 45-51
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麦类作物学报 |遗传育种 2025 , 45 (1) : 45 -51
小麦抗条锈基因Yr5Yr9Yr18分子标记的特异性评估
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孙振宇1 , 黄亮2, 黄苗苗3, 刘太国2
作者信息
  • 1.甘肃省农业科学院植物保护研究所,甘肃 兰州 730070
  • 2.中国农业科学院植物保护研究所,植物病虫害综合治理全国重点实验室,北京 100193
  • 3.青海大学农林科学院,青海 西宁 810016
通讯作者:
E-mail: (刘太国)
作者简介:

E-mail: (孙振宇)

Evaluation of Molecular Markers for Wheat Stripe Rust Resistance Genes Yr5, Yr9 and Yr18
Zhenyu SUN1 , Liang HUANG2, Miaomiao HUANG3, Taiguo LIU2
Affiliations
  • 1.Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070 China
  • 2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193 China
  • 3.Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai 810016, China
出版时间: 2025-01-15 doi: 10.7606/j.issn.1009-1041.2025.01.05
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Yr5Yr9(1B/1R)、Yr18基因的自身性状或连锁性状在中国小麦育种中具有重要的应用前景。本研究运用以Avocet S为背景的单基因近等基因系及其对应基因的载体品种,对Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记AF1/4、D15、20H,Yr18基因的分子标记csLV34、cssfr1~cssfr5进行有效性验证。结果表明,Yr5基因的特异性分子标记STS9/10、Yr9(1B/1R)基因的特异性分子标记AF1/4、D15和20H均能够准确识别不同遗传背景材料中的相应抗性基因;Yr18基因分子标记cssfr2能够准确检测Yr18的非载体材料,并检测出Avocet S*6/Yr5、Avocet S*6/Yr24、Avocet S*6/Yr27三个材料具有Yr18的等位变异。这说明Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记AF1/4、D15、20H能对目的基因进行有效检测,Yr18基因的几个分子标记结合使用不仅能够对目的基因进行有效检测,而且能够识别该位点的等位变异。

抗条锈基因  /  分子标记  /  特异性

The traits of Yr5, Yr9 (1B/1R), and Yr18 genes play an important role in wheat breeding in China, and the molecular markers STS9/10 of Yr5, AF1/4, D15, 20H of Yr9 (1B/1R) and csLV34, cssfr1~cssfr5 of Yr18 were effectively tested by applying near isogenic lines under background of Avocet S and lines with known Yr genes. The results showed that the specific molecular markers of Yr5 gene STS9/10, Yr9 (1B/1R) gene AF1/4, D15 and 20H could accurately identify the corresponding resistance genes in different genetic background materials. Yr18 gene molecular marker cssfr2 could accurately detect the materials not-carrying Yr18, and detect allelic variation of Yr18 in Avocet S*6/Yr5, Avocet S*6/Yr24, and Avocet S*6/Yr27. There fore, the linkage marker STS9/10 of Yr5 gene, the molecular markers AF1/4, D15 and 20H of Yr9 (1B/1R) gene can effectively detect the target gene, and the combination of several molecular markers of Yr18 gene not only effectively detected the target gene but also identified the allelic variation of this site.

Stripe rust resistance genes  /  Molecular markers  /  Specificity verification
孙振宇, 黄亮, 黄苗苗, 刘太国. 小麦抗条锈基因Yr5Yr9Yr18分子标记的特异性评估. 麦类作物学报, 2025 , 45 (1) : 45 -51 . DOI: 10.7606/j.issn.1009-1041.2025.01.05
Zhenyu SUN, Liang HUANG, Miaomiao HUANG, Taiguo LIU. Evaluation of Molecular Markers for Wheat Stripe Rust Resistance Genes Yr5, Yr9 and Yr18[J]. Journal of Triticeae Crops, 2025 , 45 (1) : 45 -51 . DOI: 10.7606/j.issn.1009-1041.2025.01.05
  • 国家自然科学基金项目(31660498)
  • 国家小麦产业技术体系项目(CARS-03)
  • 青海省自然科学基金项目(2022-ZJ-978Q)
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doi: 10.7606/j.issn.1009-1041.2025.01.05
  • 接收时间:2024-06-13
  • 首发时间:2026-09-11
  • 出版时间:2025-01-15
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  • 收稿日期:2024-06-13
  • 修回日期:2024-09-18
基金
国家自然科学基金项目(31660498)
国家小麦产业技术体系项目(CARS-03)
青海省自然科学基金项目(2022-ZJ-978Q)
作者信息
    1.甘肃省农业科学院植物保护研究所,甘肃 兰州 730070
    2.中国农业科学院植物保护研究所,植物病虫害综合治理全国重点实验室,北京 100193
    3.青海大学农林科学院,青海 西宁 810016

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E-mail: (刘太国)
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https://castjournals.cast.org.cn/joweb/mlzwxb/CN/10.7606/j.issn.1009-1041.2025.01.05
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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