Article(id=1305136295567717115, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136200847740945, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2026.05.05, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1761840000000, receivedDateStr=2025-10-31, revisedDate=1768838400000, revisedDateStr=2026-01-20, acceptedDate=null, acceptedDateStr=null, onlineDate=1789098341123, onlineDateStr=2026-09-11, pubDate=1778774400000, pubDateStr=2026-05-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789098341123, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789098341123, creator=13701087609, updateTime=1789098341123, updator=13701087609, issue=Issue{id=1305136200847740945, tenantId=1146029695717560320, journalId=1303687221787852879, year='2026', volume='46', issue='5', pageStart='F2', pageEnd='706', issueExtLink='null', onlineDate='null', pubDate='1778774400000', pubDateStr='2026-05-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789098318540, creator='13701087609', updateTime=1789103109806, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305156296924222402, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136200847740945, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305156296924222403, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136200847740945, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=604, endPage=612, ext={EN=ArticleExt(id=1305136295941010172, articleId=1305136295567717115, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Cloning of TaDGAT and Analysis of Its Potential Function in Anther Development in Wheat, columnId=1305133119129023428, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

To explore the potential function of diacylglycerol acyltransferase (DGAT) in the development of anther, the wheat photothermo-sensitive male sterile line BS366 was used as the material. Based on previous transcriptome sequencing results, TaDGAT (TraesCS1A02G125300) was screened and cloned from BS366. Bioinformatics analysis was employed to predict the structural and functional characteristics of the TaDGAT gene and its encoded protein. The results indicated that the gene has an open reading frame (ORF) of 1 542 bp, encoding 513 amino acids. Based on protein structure, the protein was predicted to be an O-acyltransferase. Multiple sequence alignment and phylogenetic tree analysis revealed that TaDGAT is most closely related to those in wheat and barley. Additionally, miRNAs, such as miR398 and miR169, were found to potentially interact with TaDGAT, participating in lipid metabolism processes and thereby regulating anther development. Protein-protein interaction predictions identified key interactions with O-acyltransferase and other critical proteins in lipid metabolism pathways. Subcellular localization prediction indicated that TaDGAT is located in the cell membrane and Golgi apparatus, where it participates in the formation of biological membranes. Expression pattern analysis showed that the expression of TaDGAT in different tissues, the level in anthers is low, while it is highly expressed under sterile conditions. This also suggested that the gene is closely related to pollen formation. This study provides certain references for elucidating the function of the DGAT gene in wheat.

, authors=Changjing CHEN^{1, 2}, Shengkai YING², Yingying XU², Nuo ZHAI², Zihan LIU², Yongjie LIU², Liping ZHANG^{1, 2}, Xiangzheng LIAO², Qingpeng SUN¹, Jianfang BAI², authorsList=Changjing CHEN, Shengkai YING, Yingying XU, Nuo ZHAI, Zihan LIU, Yongjie LIU, Liping ZHANG, Xiangzheng LIAO, Qingpeng SUN, Jianfang BAI, authorCompany=null, correspAuthors=Qingpeng SUN, Jianfang BAI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305136296234611453, articleId=1305136295567717115, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=小麦TaDGAT基因克隆及其在花药发育中的潜在功能分析, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

为探究二酰甘油酰基转移酶（diacylglycerol acyltransferase, DGAT） 在小麦光温敏雄性不育系花药发育中的潜在功能，以小麦光温敏核雄性不育系BS366为材料，根据前期转录组测序结果筛选出TaDGAT（TraesCS1A02G125300）基因，在BS366中克隆获得拷贝序列，利用生物信息学分析预测TaDGAT基因及其编码蛋白质的结构与功能特征。结果表明，该基因开放阅读框（ORF）为1 542 bp，共编码513个氨基酸，根据蛋白质结构推测该蛋白为O-酰基转移酶。经过多序列比对与系统发育树分析，该基因（蛋白）在小麦与大麦中亲缘关系最近，且miR398、miR169等miRNAs可能与TaDGAT互作参与脂代谢过程，进而调控花药发育；该蛋白主要与O-酰基转移酶等脂质代谢途径中的关键蛋白互作。亚细胞定位预测TaDGAT位于细胞膜和高尔基体，参与生物膜的形成。经表达模式分析，TaDGAT在各组织均有表达；其在可育环境下的花药中表达不高，但在不育环境下则高表达，说明该基因与花粉形成密切相关。本试验结果为研究DGAT基因在小麦中的功能提供一定的参考。

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