Article(id=1305136154148364800, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136112096272647, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.04.12, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1709913600000, receivedDateStr=2024-03-09, revisedDate=1715011200000, revisedDateStr=2024-05-07, acceptedDate=null, acceptedDateStr=null, onlineDate=1789098307406, onlineDateStr=2026-09-11, pubDate=1744646400000, pubDateStr=2025-04-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789098307406, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789098307406, creator=13701087609, updateTime=1789098307406, updator=13701087609, issue=Issue{id=1305136112096272647, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='4', pageStart='F2', pageEnd='562', issueExtLink='null', onlineDate='null', pubDate='1744646400000', pubDateStr='2025-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789098297379, creator='13701087609', updateTime=1789103273005, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305156981438832926, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136112096272647, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305156981438832927, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136112096272647, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=528, endPage=535, ext={EN=ArticleExt(id=1305136155993858562, articleId=1305136154148364800, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Species Identification and Pathogenicity Analysis of the Pathogen of Fusarium Crown Rot of Wheat in Shaanxi and Shanxi Provinces, columnId=1305136155880612353, journalTitle=Journal of Triticeae Crops, columnName=Disease and Pest Contro, runingTitle=null, highlight=null, articleAbstract=

In order to study the species composition and differences in pathogenicity of Fusarium crown rot of wheat pathogens in Shaanxi and Shanxi Provinces, 663 strains displaying crown rot symptoms were systematically collected from 35 sampling sites in Shaanxi and Shanxi provinces in the spring of 2023. Subsequently, five strains were randomly selected from each sampling site for pathogen isolation, and morphological observation and the molecular identification of ITS and trichothecene biosynthetic 1(Tri1) sequences were carried out. 138 strains were isolated from 175 cultures, and 85 strains of Fusarium spp. were identified by morphological and sequence analysis: including 72 strains of Fusarium pseudograminearum (84.71%), 7 strains of F. tricinctum (8.24%), 3 strains of F. graminearum (3.53%), 2 strains of F. avenaceum (2.35%), and 1 strain of F. verticillioidis (1.18%). The results showed that F. pseudograminearum was identified as the dominant pathogen causing Fusarium crown rot of wheat in Shaanxi and Shanxi Provinces. Furthermore, 32 strains of F. pseudograminearum were randomly selected to identify the pathogenicity of wheat seedlings. It was found that there were significant differences in pathogenicity among different strains. In addition, the Tri1 gene specific marker was developed in this study. Although the polymorphism of Tri1 did not directly correspond to the difference in pathogenicity, the marker effectively distinguished between F. pseudograminearum and F. graminearum. In summary, this study confirmed that the dominant strain of Fusarium crown rot of wheat in Shaanxi and Shanxi Provinces were F. pseudograminearum, and the isolates of F. pseudograminearum from different samples had significant differences in pathogenicity at seedling stage of wheat.

, authors=Jiaqi LI1, Caiye WANG1, Liuyun XI1, Fuli XIA1, Jiale ZHANG1, Chunlian LI2, Feizhou XIE3, Xiangjie YIN4, Weiliang QI5, Yantian ZHU4, Fengping YUAN1, Zhensheng KANG1, Dejun HAN2, Qingdong ZENG1, authorsList=Jiaqi LI, Caiye WANG, Liuyun XI, Fuli XIA, Jiale ZHANG, Chunlian LI, Feizhou XIE, Xiangjie YIN, Weiliang QI, Yantian ZHU, Fengping YUAN, Zhensheng KANG, Dejun HAN, Qingdong ZENG, authorCompany=null, correspAuthors=Qingdong ZENG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305136156773999108, articleId=1305136154148364800, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=陕西省和山西省小麦茎基腐病病原菌鉴定及其致病力分析, columnId=1305136156069356035, journalTitle=麦类作物学报, columnName=病虫害防治, runingTitle=null, highlight=null, articleAbstract=

为了解陕西和山西两省小麦茎基腐病害的病原菌种类组成及其致病力的差异,于2023年春季在这两个省份的35个采样点采集了663份具有茎基腐病症状的病株,对每个采样点随机选择的5株样品进行病菌分离,并开展了形态学及ITS和真菌毒素trichothecene biosynthetic 1(Tri1)序列的分子鉴定,同时随机选取32份假禾谷镰孢菌进行小麦苗期致病力鉴定。结果表明,从175份培养物分离得到138株菌系,菌系的形态学及序列特征分析鉴定得到85株镰孢菌系,其中假禾谷镰孢菌Fusarium pseudograminearum 72株(占比84.71%),三线镰孢菌F. tricinctum7株(占比8.24%),禾谷镰孢菌F. graminearum3株(占比3.53%),燕麦镰孢菌F. avenaceum2株(占比2.35%),拟轮枝镰孢菌F. verticillioides 1株(占比1.18%)。不同菌系之间致病力存在显著差异。此外,利用本研究开发的Tri1基因特异性标记能够有效地区分假禾谷镰孢菌和禾谷镰孢菌。由此可见,陕西和山西两省小麦茎基腐病优势病原菌均为假禾谷镰孢菌,不同样本分离得到的假禾谷镰孢菌菌株对小麦幼苗的致病力存在显著差异。

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陕西省和山西省小麦茎基腐病病原菌鉴定及其致病力分析
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李佳琪 1 , 王彩叶 1 , 奚柳芸 1 , 夏富丽 1 , 张嘉乐 1 , 李春莲 2 , 谢飞舟 3 , 尹祥杰 4 , 祁伟亮 5 , 朱艳天 4 , 袁凤平 1 , 康振生 1 , 韩德俊 2 , 曾庆东 1
麦类作物学报 | 病虫害防治 2025,45(4): 528-535
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麦类作物学报 |病虫害防治 2025 , 45 (4) : 528 -535
陕西省和山西省小麦茎基腐病病原菌鉴定及其致病力分析
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李佳琪1 , 王彩叶1, 奚柳芸1, 夏富丽1, 张嘉乐1, 李春莲2, 谢飞舟3, 尹祥杰4, 祁伟亮5, 朱艳天4, 袁凤平1, 康振生1, 韩德俊2, 曾庆东1
作者信息
  • 1.西北农林科技大学植物保护学院/作物抗逆与高效生产全国重点实验室,陕西 杨凌 712100
  • 2.西北农林科技大学农学院,陕西 杨凌 712100
  • 3.陕西省植物保护工作总站,陕西 西安 710003
  • 4.山西省植物保护植物检疫中心,山西 太原 030001
  • 5.陇东学院,甘肃 庆阳 74500
通讯作者:
E-mail: (曾庆东)
作者简介:

E-mail: (李佳琪)

Species Identification and Pathogenicity Analysis of the Pathogen of Fusarium Crown Rot of Wheat in Shaanxi and Shanxi Provinces
Jiaqi LI1 , Caiye WANG1, Liuyun XI1, Fuli XIA1, Jiale ZHANG1, Chunlian LI2, Feizhou XIE3, Xiangjie YIN4, Weiliang QI5, Yantian ZHU4, Fengping YUAN1, Zhensheng KANG1, Dejun HAN2, Qingdong ZENG1
Affiliations
  • 1.College of Plant Protection/State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling, Shaanxi 712100, China
  • 2.College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China
  • 3.Plant Protection Station of Shaanxi Province, Xi'an, Shaanxi 710003, China
  • 4.Plant Protection and Quarantine Station of Shanxi Province, Taiyuan, Shanxi 030001, China
  • 5.Longdong University, Qingyang, Gansu 745000, China
出版时间: 2025-04-15 doi: 10.7606/j.issn.1009-1041.2025.04.12
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为了解陕西和山西两省小麦茎基腐病害的病原菌种类组成及其致病力的差异,于2023年春季在这两个省份的35个采样点采集了663份具有茎基腐病症状的病株,对每个采样点随机选择的5株样品进行病菌分离,并开展了形态学及ITS和真菌毒素trichothecene biosynthetic 1(Tri1)序列的分子鉴定,同时随机选取32份假禾谷镰孢菌进行小麦苗期致病力鉴定。结果表明,从175份培养物分离得到138株菌系,菌系的形态学及序列特征分析鉴定得到85株镰孢菌系,其中假禾谷镰孢菌Fusarium pseudograminearum 72株(占比84.71%),三线镰孢菌F. tricinctum7株(占比8.24%),禾谷镰孢菌F. graminearum3株(占比3.53%),燕麦镰孢菌F. avenaceum2株(占比2.35%),拟轮枝镰孢菌F. verticillioides 1株(占比1.18%)。不同菌系之间致病力存在显著差异。此外,利用本研究开发的Tri1基因特异性标记能够有效地区分假禾谷镰孢菌和禾谷镰孢菌。由此可见,陕西和山西两省小麦茎基腐病优势病原菌均为假禾谷镰孢菌,不同样本分离得到的假禾谷镰孢菌菌株对小麦幼苗的致病力存在显著差异。

小麦茎基腐病  /  病原菌鉴定  /  假禾谷镰孢菌  /  致病力分析

In order to study the species composition and differences in pathogenicity of Fusarium crown rot of wheat pathogens in Shaanxi and Shanxi Provinces, 663 strains displaying crown rot symptoms were systematically collected from 35 sampling sites in Shaanxi and Shanxi provinces in the spring of 2023. Subsequently, five strains were randomly selected from each sampling site for pathogen isolation, and morphological observation and the molecular identification of ITS and trichothecene biosynthetic 1(Tri1) sequences were carried out. 138 strains were isolated from 175 cultures, and 85 strains of Fusarium spp. were identified by morphological and sequence analysis: including 72 strains of Fusarium pseudograminearum (84.71%), 7 strains of F. tricinctum (8.24%), 3 strains of F. graminearum (3.53%), 2 strains of F. avenaceum (2.35%), and 1 strain of F. verticillioidis (1.18%). The results showed that F. pseudograminearum was identified as the dominant pathogen causing Fusarium crown rot of wheat in Shaanxi and Shanxi Provinces. Furthermore, 32 strains of F. pseudograminearum were randomly selected to identify the pathogenicity of wheat seedlings. It was found that there were significant differences in pathogenicity among different strains. In addition, the Tri1 gene specific marker was developed in this study. Although the polymorphism of Tri1 did not directly correspond to the difference in pathogenicity, the marker effectively distinguished between F. pseudograminearum and F. graminearum. In summary, this study confirmed that the dominant strain of Fusarium crown rot of wheat in Shaanxi and Shanxi Provinces were F. pseudograminearum, and the isolates of F. pseudograminearum from different samples had significant differences in pathogenicity at seedling stage of wheat.

Fusarium crown rot  /  Pathogen identification  /  Fusarium pseudograminearum  /  Pathogenicity analysis
李佳琪, 王彩叶, 奚柳芸, 夏富丽, 张嘉乐, 李春莲, 谢飞舟, 尹祥杰, 祁伟亮, 朱艳天, 袁凤平, 康振生, 韩德俊, 曾庆东. 陕西省和山西省小麦茎基腐病病原菌鉴定及其致病力分析. 麦类作物学报, 2025 , 45 (4) : 528 -535 . DOI: 10.7606/j.issn.1009-1041.2025.04.12
Jiaqi LI, Caiye WANG, Liuyun XI, Fuli XIA, Jiale ZHANG, Chunlian LI, Feizhou XIE, Xiangjie YIN, Weiliang QI, Yantian ZHU, Fengping YUAN, Zhensheng KANG, Dejun HAN, Qingdong ZENG. Species Identification and Pathogenicity Analysis of the Pathogen of Fusarium Crown Rot of Wheat in Shaanxi and Shanxi Provinces[J]. Journal of Triticeae Crops, 2025 , 45 (4) : 528 -535 . DOI: 10.7606/j.issn.1009-1041.2025.04.12
  • 小麦茎基腐病抗病性鉴定
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doi: 10.7606/j.issn.1009-1041.2025.04.12
  • 接收时间:2024-03-09
  • 首发时间:2026-09-11
  • 出版时间:2025-04-15
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  • 收稿日期:2024-03-09
  • 修回日期:2024-05-07
基金
小麦茎基腐病抗病性鉴定
作者信息
    1.西北农林科技大学植物保护学院/作物抗逆与高效生产全国重点实验室,陕西 杨凌 712100
    2.西北农林科技大学农学院,陕西 杨凌 712100
    3.陕西省植物保护工作总站,陕西 西安 710003
    4.山西省植物保护植物检疫中心,山西 太原 030001
    5.陇东学院,甘肃 庆阳 74500

通讯作者:

E-mail: (曾庆东)
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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