Article(id=1305136009063190841, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305135949499884251, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2026.06.06, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1758124800000, receivedDateStr=2025-09-18, revisedDate=1761667200000, revisedDateStr=2025-10-29, acceptedDate=null, acceptedDateStr=null, onlineDate=1789098272815, onlineDateStr=2026-09-11, pubDate=1781452800000, pubDateStr=2026-06-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789098272815, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789098272815, creator=13701087609, updateTime=1789098272815, updator=13701087609, issue=Issue{id=1305135949499884251, tenantId=1146029695717560320, journalId=1303687221787852879, year='2026', volume='46', issue='6', pageStart='F2', pageEnd='850', issueExtLink='null', onlineDate='null', pubDate='1781452800000', pubDateStr='2026-06-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789098258614, creator='13701087609', updateTime=1789103086332, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305156198454547374, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305135949499884251, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305156198454547375, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305135949499884251, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=754, endPage=767, ext={EN=ArticleExt(id=1305136009277100346, articleId=1305136009063190841, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=SSR Marker Based Analysis for Identification and Genetic Diversity of Hulless Barley Varieties, columnId=1305133119129023428, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

To establish a molecular identification system and DNA fingerprint database for hulless barley (Hordeum vulgare L. var. nudum Hook. f.) varieties in China, this study aimed to screen a set of SSR core primers suitable for national variety identification standards, thereby providing technical support for variety authentication, management, and new variety protection. Based on previously reported SSR markers of hulless barley, PCR amplification, marker screening, and validation were performed across diverse varieties, followed by analyses of genetic diversity and discrimination efficiency. Using 155 hulless barley accessions, 200 SSR primer pairs were subjected to polymorphism screening. Twenty-one core primers were selected based on criteria including clear and easily interpretable peak patterns, high polymorphism (mean PIC=0.68), amplification stability, and even chromosomal distribution. Reference varieties (e.g., Yunqing 4, Diqing 4, and Kunlun 8) were employed to validate the stability and reliability of these primers. The constructed DNA fingerprint database for 155 accessions detected 137 alleles, ranging from 2 to 10 per locus (mean=6.52). The polymorphism information content (PIC) values varied between 0.39 and 0.85 (mean=0.68), with 95.24% of loci classified as highly polymorphic (PIC>0.5). These 21 primers achieved a discrimination efficiency of 99.991%, distinguishing 153 out of 155 accessions. Cluster analysis, population structure analysis, and principal component analysis classified the 154 accessions into three distinct groups, revealing rich genetic diversity and simple genetic backgrounds in 45.81% of the accessions. The SSR-based identification system developed in this study demonstrated high accuracy and practical applicability, enabling authenticity verification, auxiliary screening of approximate varieties in DUS testing, and intellectual property protection. Furthermore, the DNA fingerprint database and genetic diversity analysis provide valuable molecular-level insights for future hulless barley breeding programs.

, authors=Cailing TENG^{1, 2}, Xiaohong YANG^{1, 2}, Ruonan JIN³, Kaixi ZHANG³, Yongying FA^{1, 2}, Chunfang ZHAO⁴, Abdelfattah Mohammed Abdelfattah Nagy⁵, Ruixi HAN³, Yanfang LIU^{1, 2}, authorsList=Cailing TENG, Xiaohong YANG, Ruonan JIN, Kaixi ZHANG, Yongying FA, Chunfang ZHAO, Abdelfattah Mohammed Abdelfattah Nagy, Ruixi HAN, Yanfang LIU, authorCompany=null, correspAuthors=Ruixi HAN, Yanfang LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305136009532952892, articleId=1305136009063190841, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=基于SSR分子标记的青稞品种鉴定及遗传多样性分析, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

为了筛选一套适合中国青稞品种鉴定标准的SSR核心引物，并建立青稞品种分子鉴定体系和DNA指纹数据库，基于国内外已报道的青稞SSR标记，对155个青稞品种进行PCR扩增和标记筛选验证，并分析其遗传多样性和鉴别效率。结果表明，从200对SSR引物中，筛选出峰型简单易读取、多态性好、扩增稳定性高、染色体上分布均匀的核心引物21对，用云青4号、迪青4号、昆仑8号等31个参照品种验证了21对核心引物的稳定性和可靠性。利用21对核心引物构建了155个青稞品种的指纹数据库，并检测到137个等位变异，每对引物等位变异的范围为2~10个，平均值为6.52个；PIC值范围为0.39~0.85，平均值为0.68，高多态性位点占比95.24%；21对核心引物可区分155个品种中的153个品种，引物鉴别效率为99.991%。通过聚类分析、群体结构分析和主成分分析，将154个青稞品种分为3个类群，品种间遗传多样性丰富，45.81%的品种遗传背景简单。由此可见，基于SSR分子标记建立的青稞品种鉴定体系鉴定效果好，应用性强，可用于青稞品种真实性鉴定、辅助DUS测试近似品种筛选、新品种权保护等领域；155个青稞品种的DNA指纹数据库和遗传多样性分析结果可为未来青稞育种提供一定参考。

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