麦类作物学报

TaNAC2基因对小麦低温耐受性和农艺性状的影响

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郝淑女 ¹^,² , 于宛可 ² , 罗佳 ¹^,² , 马新 ¹^,² , 杨柏松 ² , 王含 ² , 杨淑颖 ² , 张慧慧 ² , 王豆 ² , 房智羽 ² , 刘含章 ² , 张茹萱 ¹^,² , 马守才 ¹ , 孙果忠 ²

麦类作物学报 | 遗传育种 2026,46(10): 1303-1312

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麦类作物学报 |遗传育种 2026 , 46 (10) : 1303 -1312

TaNAC2基因对小麦低温耐受性和农艺性状的影响

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郝淑女¹^,², 于宛可², 罗佳¹^,², 马新¹^,², 杨柏松², 王含², 杨淑颖², 张慧慧², 王豆², 房智羽², 刘含章², 张茹萱¹^,², 马守才¹, 孙果忠²

作者信息

^1.西北农林科技大学农学院，陕西杨凌　712100

^2.作物分子育种国家工程研究中心，中国农业科学院作物科学研究所，北京　100081

通讯作者:

E-mail：sunguozhong@caas.cn（孙果忠）

mashoucai@nwsuaf.edu.cn（马守才）

作者简介:

E-mail：haoshunv514@126.com（郝淑女）

18963109926@163.com（于宛可）

Effect of the TaNAC2 Gene on Freeze Tolerance and Agronomic Traits of Wheat

Shunü Hao¹^,², Wanke Yu², Jia Luo¹^,², Xin Ma¹^,², Baisong Yang², Han Wang², Shuying Yang², Huihui Zhang², Dou Wang², Zhiyu Fang², Hanzhang Liu², Ruxuan Zhang¹^,², Shoucai Ma¹, Guozhong Sun²

Affiliations

^1.College of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China

^2.National Engineering Research Center for Crop Molecular Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

出版时间: 2026-10-15 doi: 10.7606/j.issn.1009-1041.2026.10.04

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摘要

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NAC转录因子在协调植物生长发育和逆境胁迫反应中发挥着重要的调控作用。为探究TaNAC2基因在小麦低温胁迫响应和生长发育中的功能，本研究构建了以Fielder为转化受体的TaNAC2转基因和基因编辑株系，在培养箱和温室环境下进行了低温胁迫耐受性、生长发育和农艺性状的比较。结果表明，在-5 ℃低温胁迫处理10 h后，与Fielder相比，其T₃代转基因株系OE8、OE11、OE14的TaNAC2基因相对表达水平均极显著提高（P<0.01），相对电导率显著降低（P<0.05）；幼苗的存活率和冻害指数分别升高降低，但均无显著差异。T₄代基因编辑株系GE45（aabbdd）的TaNAC2基因相对表达水平显著降低（P<0.05），幼苗的存活率降低，相对电导率和冻害指数升高，此3个竹理指标也均无显著差异；GE91（aaBBDD）的TaNAC2基因相对表达水平、相对电导率、幼苗存活率和冻害指数均与Fielder相近。在温室盆栽条件下，与Fielder相比，除OE11的播种至抽穗天数显著降低（P<0.05）外，OE8、OE14、GE45、GE91的株高、分蘖、穗粒数等农艺性状均无显著差异。综上所述，TaNAC2基因正向调控小麦的低温胁迫耐受性，但对农艺性状无明显影响。

关键词

小麦 / TaNAC2 / 低温胁迫 / 农艺性状 / 基因过表达 / 基因编辑

Abstract

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NAC transcription factors are pivotal in regulating plant growth, development, and stress responses. This study aims to investigate the role of the TaNAC2 gene in wheat's adaptation to low-temperature stress and its growth development. To achieve this, we developed TaNAC2 gene overexpression and gene-edited lines using Fielder as the transformation recipient. Subsequently, we evaluated cold stress tolerance, growth development, and agronomic traits in both controlled incubator and greenhouse. The results showed that after 10 hours of low-temperature stress treatment at -5 ℃, compared to Fielder, the relative expression level of TaNAC2 genes in the T₃ overexpression lines OE8, OE11, and OE14 was significantly increased (P<0.01), while relative conductivity was significantly reduced (P<0.05); seedling survival rates increased and frost damage indices decreased, but neither showed significant differences. In the T₄-generation gene-edited line GE45 (aabbdd), the relative expression level of the TaNAC2 gene was significantly reduced (P<0.05); seedling survival rate decreased, and relative conductivity and frost injury index increased, but none of these showed significant differences; in GE91 (aaBBDD), the relative expression level of the TaNAC2 gene, relative conductivity, seedling survival rate, and frost injury index were all similar to Fielder. Under greenhouse pot conditions, compared with Fielder, except that the days from sowing to heading of OE11 were significantly reduced (P<0.05), there were no significant differences in plant height, tiller number, and spikelet number of OE8, OE14, GE45, and GE91. In summary, the TaNAC2 gene positively regulates wheat's tolerance to low-temperature stress, but has no obvious effect on agronomic traits.

Key words

Triticum aestivum L. / TaNAC2 / Low temperature stress / Agronomic traits / Gene over expression / Gene editing

引用本文

郝淑女, 于宛可, 罗佳, 马新, 杨柏松, 王含, 杨淑颖, 张慧慧, 王豆, 房智羽, 刘含章, 张茹萱, 马守才, 孙果忠. TaNAC2基因对小麦低温耐受性和农艺性状的影响. 麦类作物学报, 2026 , 46 (10) : 1303 -1312 . DOI: 10.7606/j.issn.1009-1041.2026.10.04

Shunü Hao, Wanke Yu, Jia Luo, Xin Ma, Baisong Yang, Han Wang, Shuying Yang, Huihui Zhang, Dou Wang, Zhiyu Fang, Hanzhang Liu, Ruxuan Zhang, Shoucai Ma, Guozhong Sun. Effect of the TaNAC2 Gene on Freeze Tolerance and Agronomic Traits of Wheat[J]. Journal of Triticeae Crops, 2026 , 46 (10) : 1303 -1312 . DOI: 10.7606/j.issn.1009-1041.2026.10.04

基金

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国家重大科技专项(2023ZD0402402)
财政部和农业农村部小麦产业技术体系项目(CARS-03)
国家重点研发计划项目(2021YFD1200600)

参考文献

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2026年第46卷第10期

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文章信息

doi: 10.7606/j.issn.1009-1041.2026.10.04

接收时间：2026-04-06
首发时间：2026-10-27
出版时间：2026-10-15

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出版历史

收稿日期：2026-04-06
修回日期：2026-06-19

基金

国家重大科技专项(2023ZD0402402)

财政部和农业农村部小麦产业技术体系项目(CARS-03)

国家重点研发计划项目(2021YFD1200600)

作者信息

^1.西北农林科技大学农学院，陕西杨凌　712100

^2.作物分子育种国家工程研究中心，中国农业科学院作物科学研究所，北京　100081

通讯作者:

E-mail：sunguozhong@caas.cn（孙果忠）

mashoucai@nwsuaf.edu.cn（马守才）

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https://castjournals.cast.org.cn/joweb/mlzwxb/CN/10.7606/j.issn.1009-1041.2026.10.04

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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