麦类作物学报

629份河南省小麦种质的条锈病抗性评价及抗病基因分析

PDF下载

杜习军 ¹ , 赵明忠 ¹ , 陈艳艳 ¹ , 王松峰 ¹ , 高崇 ¹ , 巩晨 ¹ , 胡琳 ¹ , 许为钢 ¹^,² , 齐学礼 ¹

麦类作物学报 | 遗传育种 2026,46(10): 1271-1279

收起

麦类作物学报 |遗传育种 2026 , 46 (10) : 1271 -1279

629份河南省小麦种质的条锈病抗性评价及抗病基因分析

全屏

杜习军¹, 赵明忠¹, 陈艳艳¹, 王松峰¹, 高崇¹, 巩晨¹, 胡琳¹, 许为钢¹^,², 齐学礼¹

作者信息

^1.河南省作物分子育种研究院/河南省小麦种质资源与遗传改良重点实验室/神农种业实验室，河南郑州　450002

^2.河南农业大学农学院，河南郑州　450046

通讯作者:

E-mail：xueliqi888@163.com（齐学礼）

作者简介:

E-mail：duxijun2015@126.com（杜习军）

zmingzhongz@163.com（赵明忠）

Evaluation of Stripe Rust Resistance and Analysis of Resistance Genes in 629 Henan Wheat Germplasms

Xijun Du¹, Mingzhong Zhao¹, Yanyan Chen¹, Songfeng Wang¹, Chong Gao¹, Chen Gong¹, Lin Hu¹, Weigang Xu¹^,², Xueli Qi¹

Affiliations

^1.Henan Academy of Crops Molecular Breeding/Key Laboratory for Wheat Germplasm Resources and Genetic Improvement in Henan Province/The Shennong Laboratory, Zhengzhou, Henan 450002, China

^2.College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450046, China

出版时间: 2026-10-15 doi: 10.7606/j.issn.1009-1041.2026.10.01

文章导航

摘要

收起

近年来，由于条锈菌小种CYR34的流行，河南省小麦条锈病发生呈现上升趋势。为明确河南省小麦对CYR34等优势小种的抗性，本研究鉴定了629份河南省小麦种质苗期和成株期对CYR34等优势小种的抗性，并利用KASP分子标记对抗性基因Yr5、Yr7、Yr15、Yr18、Yr78、Yr81、YrZH84和YrZM1354进行检测，系统评价其抗性水平与遗传基础。结果表明，供试的629份小麦种质对CYR34等当前流行小种的抗性水平整体偏低，育成品种的成株期抗性显著弱于农家种。利用分子标记未检测到携带Yr5、Yr15、Yr81的品种；成株期抗性基因Yr18在农家种中检出频率为93.03%，而在育成品种中仅占1.95%；仅育成品种携带YrZM1354、YrZH84。通过遗传效应分析，Yr18对小麦抗条锈性表型贡献最大，而YrZM1354单基因效应最强，聚合YrZH84与YrZM1354可提升苗期抗条锈性。基于以上结果，建议河南小麦抗病育种应回补Yr18，加速利用YrZM1354等新抗源，推进多基因聚合育种，以拓宽抗病遗传基础，培育持久抗病新品种。

关键词

小麦条锈病 / KASP标记 / 分子检测 / 基因聚合 / 抗病育种

Abstract

收起

In recent years, the occurrence of wheat stripe rust in Henan Province has shown an increasing trend due to the prevalence race CYR34 of the stripe rust. To clarify the resistance of wheat germplasms in Henan against dominant races including CYR34, this study systematically evaluated the resistance and genetic basis of 629 local wheat germplasms at both seedling and adult plant stages. Stripe rust resistance genes (Yr5, Yr7, Yr15, Yr18, Yr78, Yr81, YrZH84, and YrZM1354) were genotyped using KASP markers. The results revealed a generally low resistance level in the tested wheat population to currently prevalent races including CYR34, with cultivars exhibiting significantly weaker adult-plant stage resistance than landraces. The adult-plant stage resistance gene Yr18 was detected at a frequency of 93.03% in the landraces, whereas it was found in only 1.95% of cultivars. Meanwhile, cultivars carried new resistant genes YrZM1354 and YrZH84. Further genetic effect analysis showed that Yr18 contributed the most to the wheat population’s phenotypic resistance, while YrZM1354 had the strongest single-gene effect. Combining YrZH84 and YrZM1354 can enhance seedling resistance. Based on these testing results, it is recommended that wheat disease resistance breeding in Henan should focus on reintroducing Yr18, accelerating the deployment of new genes such as YrZM1354, and promoting multi-gene pyramiding to broaden the genetic basis of resistance and develop durable-resistant varieties. This study provides valuable resistant source materials, genetic insights, and strategic guidance for wheat disease resistance breeding in Henan Province.

Key words

Wheat stripe rust / KASP marker / Molecular detection / Gene pyramiding / Disease-resistant breeding

引用本文

杜习军, 赵明忠, 陈艳艳, 王松峰, 高崇, 巩晨, 胡琳, 许为钢, 齐学礼. 629份河南省小麦种质的条锈病抗性评价及抗病基因分析. 麦类作物学报, 2026 , 46 (10) : 1271 -1279 . DOI: 10.7606/j.issn.1009-1041.2026.10.01

Xijun Du, Mingzhong Zhao, Yanyan Chen, Songfeng Wang, Chong Gao, Chen Gong, Lin Hu, Weigang Xu, Xueli Qi. Evaluation of Stripe Rust Resistance and Analysis of Resistance Genes in 629 Henan Wheat Germplasms[J]. Journal of Triticeae Crops, 2026 , 46 (10) : 1271 -1279 . DOI: 10.7606/j.issn.1009-1041.2026.10.01

基金

收起

国家自然科学基金青年项目(32501915)
河南省科技研发计划联合基金（优势学科培育类）培育项目(242301420124)
河南省农业科学院博士科研启动费项目(2026BX16; 2026BX15)
河南省农业科学院优秀青年科技基金项目(2024YQ09)
国家小麦产业技术体系专项(CARS-03-7)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

[1]

Tester M, Langridge P. Breeding technologies to increase crop production in a changing world[J]. Science, 2010, 327(5967): 818.

[2]

Wellings C R. Global status of stripe rust: A review of historical and current threats[J]. Euphytica, 2011, 179(1): 129.

[3]

Chen X M. Epidemiology and control of stripe rust[Puccinia striiformis f. sp. tritici] on wheat[J]. Canadian Journal of Plant Pathology, 2005, 27(3): 314.

[4]

Du X J, Xu W G, Peng C J, et al. Identification and validation of a novel locus, Qpm-3BL, for adult plant resistance to powdery mildew in wheat using multilocus GWAS[J]. BMC Plant Biology, 2021, 21(1): 357.

[5]

于思勤, 彭红, 李金锁, 等. 2017年河南省小麦条锈病流行的原因分析及应对措施[J]. 中国植保导刊, 2017, 37(12): 34. Yu S Q, Peng H, Li J S, et al. Analysis on wheat stripe rust epidemic causes and its countermeasure in Henan Province in 2017[J]. China Plant Protection, 2017, 37(12): 34.

[6]

张传伟, 巩中军, 郭姝辰, 等. 近30年河南省小麦病虫草害发生演变特点分析[J]. 中国植保导刊, 2022, 42(3): 29. Zhang C W, Gong Z J, Guo S C, et al. Spatial patterns and temporal trends of wheat pests in Henan Province in the past 30 years[J]. China Plant Protection, 2022, 42(3): 29.

[7]

Flor H H. Host-parasite interaction in flax rust-its genetics and other implications[J]. Phytopathology, 2025, 115(8V): 680.

[8]

Flor H H. Current status of the gene-for-gene concept[J]. Annual Review of Phytopathology, 1971, 9: 275.

[9]

Lan C X, Zhang Y L, Herrera-Foessel S A, et al. Identification and characterization of pleiotropic and co-located resistance loci to leaf rust and stripe rust in bread wheat cultivar Sujata[J]. Theoretical and Applied Genetics, 2015, 128(3): 549.

[10]

Chen X M. Review article: High-temperature adult-plant resistance, key for sustainable control of stripe rust[J]. American Journal of Plant Sciences, 2013, 4(3): 608.

[11]

Li J B, Dundas I, Dong C M, et al. Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat[J]. Theoretical and Applied Genetics, 2020, 133(4): 1095.

[12]

Wan A M, Wang X J, Kang Z S, et al. Variability of the stripe rust pathogen[M]//Stripe Rust. Dordrecht: Springer Netherlands, 2017: 35.

[13]

Feng J Y, Wang M N, See D R, et al. Characterization of novel gene Yr79 and four additional quantitative trait loci for all-stage and high-temperature adult-plant resistance to stripe rust in spring wheat PI 182103[J]. Phytopathology, 2018, 108(6): 737.

[14]

Nsabiyera V, Bariana H S, Qureshi N, et al. Characterisation and mapping of adult plant stripe rust resistance in wheat accession Aus27284[J]. Theoretical and Applied Genetics, 2018, 131(7): 1459.

[15]

Jin H L, Zhang H P, Zhao X Y, et al. Identification of a suppressor for the wheat stripe rust resistance gene Yr81 in Chinese wheat landrace Dahongpao[J]. Theoretical and Applied Genetics, 2023, 136(4): 67.

[16]

Pakeerathan K, Bariana H, Qureshi N, et al. Identification of a new source of stripe rust resistance Yr82 in wheat[J]. Theoretical and Applied Genetics, 2019, 132(11): 3169.

[17]

Sharma D, Avni R, Gutierrez-Gonzalez J, et al. A single NLR gene confers resistance to leaf and stripe rust in wheat[J]. Nature Communications, 2024, 15: 9925.

[18]

Wan A M, Chen X M. Virulence characterization of Puccinia striiformis f. sp. tritici using a new set of Yr single-gene line differentials in the United States in 2010[J]. Plant Disease, 2014, 98(11): 1534.

[19]

Yuan C L, Jiang H, Wang H G, et al. Distribution, frequency and variation of stripe rust resistance loci Yr10, Lr34/Yr18 and Yr36 in Chinese wheat cultivars[J]. Journal of Genetics and Genomics, 2012, 39(11): 587.

[20]

Li Z F, Zheng T C, He Z H, et al. Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B[J]. Theoretical and Applied Genetics, 2006, 112(6): 1098.

[21]

Du X J, Hu L, Xu W G, et al. Mapping, validation, and development of markers for the major adult plant stripe rust resistance locus QYr. hnk-7DL in Henan wheat[J]. BMC Plant Biology, 2025, 25(1): 808.

[22]

焦文娟, 白斌, 谢克莱·依拉木, 等. 295份国内外小麦种质资源条锈病抗性评价及抗病基因分子检测[J]. 作物学报, 2025, 51(11): 2886. Jiao W J, Bai B, Xiekelai Y L M, et al. Evaluation of stripe rust resistance in 295 domestic and foreign wheat germplasm resources and molecular detection of resistance genes[J]. Acta Agronomica Sinica, 2025, 51(11): 2886.

[23]

Gessese M, Bariana H, Wong D, et al. Molecular mapping of stripe rust resistance gene Yr81 in a common wheat Landrace Aus27430[J]. Plant Disease, 2019, 103(6): 1166.

[24]

Wu J H, Zeng Q D, Wang Q L, et al. SNP-based pool genotyping and haplotype analysis accelerate fine-mapping of the wheat genomic region containing stripe rust resistance gene Yr26[J]. Theoretical and Applied Genetics, 2018, 131(7): 1481.

[25]

Line R F, Qayoum A. Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968—87: 1788[R]. US Department of Agriculture, Agricultural Research Service, 1992.

[26]

Ma Z Q, Sorrells M E, Tanksley S D. RFLP markers linked to powdery mildew resistance genes PM₁, Pm2, Pm3, and Pm4 in wheat[J]. Genome, 1994, 37(5): 871.

[27]

Marchal C, Zhang J P, Zhang P, et al. BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust[J]. Nature Plants, 2018, 4(9): 662.

[28]

Ramirez-Gonzalez R H, Segovia V, Bird N, et al. RNA-Seq bulked segregant analysis enables the identification of high-resolution genetic markers for breeding in hexaploid wheat[J]. Plant Biotechnology Journal, 2015, 13(5): 613.

[29]

Neelam K, Brown-Guedira G, Huang L. Development and validation of a breeder-friendly KASPar marker for wheat leaf rust resistance locus Lr₂₁[J]. Molecular Breeding, 2013, 31(1): 233.

[30]

Dong Z Z, Hegarty J M, Zhang J L, et al. Validation and characterization of a QTL for adult plant resistance to stripe rust on wheat chromosome arm 6BS (Yr78)[J]. Theoretical and Applied Genetics, 2017, 130(10): 2127.

[31]

Gessese M, Bariana H, Wong D, et al. Molecular mapping of stripe rust resistance gene Yr81 in a common wheat landrace Aus27430[J]. Plant Disease, 2019, 103(6): 1166.

[32]

Li Z F, Zheng T C, He Z H, et al. Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B[J]. Theoretical and Applied Genetics, 2006, 112(6): 1098.

2026年第46卷第10期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7606/j.issn.1009-1041.2026.10.01

接收时间：2026-02-11
首发时间：2026-10-27
出版时间：2026-10-15

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2026-02-11
修回日期：2026-04-01

基金

国家自然科学基金青年项目(32501915)

河南省科技研发计划联合基金（优势学科培育类）培育项目(242301420124)

河南省农业科学院博士科研启动费项目(2026BX16; 2026BX15)

河南省农业科学院优秀青年科技基金项目(2024YQ09)

国家小麦产业技术体系专项(CARS-03-7)

作者信息

^1.河南省作物分子育种研究院/河南省小麦种质资源与遗传改良重点实验室/神农种业实验室，河南郑州　450002

^2.河南农业大学农学院，河南郑州　450046

通讯作者:

E-mail：xueliqi888@163.com（齐学礼）

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/mlzwxb/CN/10.7606/j.issn.1009-1041.2026.10.01

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT