麦类作物学报

小麦抗白粉病基因PmHYM的精细定位与功能标记开发

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林芝欣 ¹^,²^,³ , 陈雨心 ¹^,²^,³ , 亢宁宁 ¹^,²^,³ , 陈芳 ¹^,²^,³ , 许峰 ¹ , 付必胜 ²^,³ , 吴纪中 ²^,³

麦类作物学报 | 遗传育种 2026,46(7): 885-896

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麦类作物学报 |遗传育种 2026 , 46 (7) : 885 -896

小麦抗白粉病基因PmHYM的精细定位与功能标记开发

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林芝欣¹^,²^,³, 陈雨心¹^,²^,³, 亢宁宁¹^,²^,³, 陈芳¹^,²^,³, 许峰¹, 付必胜²^,³, 吴纪中²^,³

作者信息

^1．安徽科技工程大学农学院，安徽滁州 233100

^2．生物育种钟山实验室，江苏南京 210014

^3．江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室，江苏南京 210014

通讯作者:

E-mail: xuf@ahstu.edu.cn（许峰）；

fbs1006@126.com（付必胜）

作者简介:

E-mail: 2429298377@qq.com（林芝欣）

Fine Mapping and Development of Functional Markers for the Powdery Mildew Resistance Gene PmHYM in Wheat

Zhixin LIN¹^,²^,³, Yuxin CHEN¹^,²^,³, Ningning KANG¹^,²^,³, Fang CHEN¹^,²^,³, Feng XU¹, Bisheng FU²^,³, Jizhong WU²^,³

Affiliations

^1.Anhui Science and Technology University, Chuzhou, Anhui 233100, China

^2.Zhongshan Biological Breeding Laboratory, Nanjing, Jiangsu 210014, China

^3.Institute of Crop Germplasm and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China

出版时间: 2026-07-15 doi: 10.7606/j.issn.1009-1041.2026.07.05

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摘要

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由禾本科布氏白粉菌小麦专化型（Blumeria graminis f. sp. tritici，Bgt）引起的小麦白粉病严重影响小麦的产量与品质，挖掘新的抗白粉病基因并开发功能标记对小麦抗病分子育种具有重要意义。本研究先以红蚰麦为抗病亲本、宁麦资119为感病亲本构建F₂遗传分离群体，整合抗性鉴定表型数据、诊断性分子标记开发与重组体分析，对小麦抗白粉病基因PmHYM进行精细定位；通过序列比对、染色体分选测序、表达模式及拷贝数检测，解析候选基因的序列特征；基于功能SNP开发KASP标记，通过回交转育评估基因育种潜力。结果表明，PmHYM精细定位至7BL染色体的Xmp1410~Xmp1396标记之间，0.5 cM遗传区间内，对应中国春参考基因组的310 kb物理区间，该区间预测有2个卷曲螺旋-核苷酸结合-富亮氨酸重复（coiled-coil nucleotide-binding leucine-rich repeat type, CNL）类候选基因；红蚰麦携带的这2个候选基因序列与复壮30的完全一致；红蚰麦与复壮30对白粉菌生理小种B16抗谱存在差异，该差异与基因序列、拷贝数变异无关；开发了PmHYM高通量诊断性KASP分子标记Xmp1394，可快速追踪PmHYM；获得了携带PmHYM的转育植株，农艺性状与轮回亲本相比无明显差异，具备较高的育种利用潜力。

关键词

小麦白粉病 / 遗传标记 / KASP标记

Abstract

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Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), severely influences wheat yield and grain quality. Mining novel powdery mildew resistance (Pm) genes and developing functional markers is of great significance for molecular breeding of disease-resistant wheat. In this study, an F₂ segregating population was first constructed using Hongyoumai as the resistant parent and Ningmaizi 119 as the susceptible parent. By integrating phenotypic data from resistance identification, diagnostic molecular marker development, and recombinant analysis, the wheat powdery mildew resistance gene PmHYM was finely mapped. Subsequently, sequence characteristics of the candidate genes were analyzed through sequence alignment, flow sorting and chromosome sequencing, expression pattern analysis, and copy number variation detection. Finally, KASP markers were developed based on functional SNP, and the breeding potential of the gene was evaluated via backcross breeding. The results showed that PmHYM was fine-mapped to a 0.5 cM genetic interval between markers Xmp1410 and Xmp1396 on chromosome 7BL, corresponding to a 310 kb physical interval in the Chinese Spring reference genome. Two coiled-coil nucleotide-binding leucine-rich repeat (CNL)-type proteins were predicted within this region. The sequences of these two genes in Hongyoumai were identical to those in Fuzhuang 30. The difference in the resistance spectrum to Bgt isolate B16, between Hongyoumai and Fuzhuang 30 was independent of gene sequence variations or copy number variations. A high-throughput diagnostic KASP marker Xmp1394 for PmHYM was successfully developed, enabling rapid tracking of PmHYM. Backcross-derived lines carrying PmHYM exhibited no significant differences in agronomic traits compared with the recurrent parent, suggesting as high potential for breeding application.

Key words

Blumeria graminus / Genetic marker / KASP marker

引用本文

林芝欣, 陈雨心, 亢宁宁, 陈芳, 许峰, 付必胜, 吴纪中. 小麦抗白粉病基因PmHYM的精细定位与功能标记开发. 麦类作物学报, 2026 , 46 (7) : 885 -896 . DOI: 10.7606/j.issn.1009-1041.2026.07.05

Zhixin LIN, Yuxin CHEN, Ningning KANG, Fang CHEN, Feng XU, Bisheng FU, Jizhong WU. Fine Mapping and Development of Functional Markers for the Powdery Mildew Resistance Gene PmHYM in Wheat[J]. Journal of Triticeae Crops, 2026 , 46 (7) : 885 -896 . DOI: 10.7606/j.issn.1009-1041.2026.07.05

基金

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国家自然科学基金项目(31971889)
生物育种钟山实验室项目(ZSBBL-KY2023-02-1)
江苏省重点研发计划（现代农业）项目(BE2022346)

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排序方式：

2026年第46卷第7期

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文章信息

doi: 10.7606/j.issn.1009-1041.2026.07.05

接收时间：2025-12-12
首发时间：2026-09-11
出版时间：2026-07-15

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出版历史

收稿日期：2025-12-12
修回日期：2026-04-07

基金

国家自然科学基金项目(31971889)

生物育种钟山实验室项目(ZSBBL-KY2023-02-1)

江苏省重点研发计划（现代农业）项目(BE2022346)

作者信息

^1．安徽科技工程大学农学院，安徽滁州 233100

^2．生物育种钟山实验室，江苏南京 210014

^3．江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室，江苏南京 210014

通讯作者:

E-mail: xuf@ahstu.edu.cn（许峰）；

fbs1006@126.com（付必胜）

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https://castjournals.cast.org.cn/joweb/mlzwxb/CN/10.7606/j.issn.1009-1041.2026.07.05

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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