Article(id=1305136214613451740, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136200847740945, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2026.05.01, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1758729600000, receivedDateStr=2025-09-25, revisedDate=1768752000000, revisedDateStr=2026-01-19, acceptedDate=null, acceptedDateStr=null, onlineDate=1789098321822, onlineDateStr=2026-09-11, pubDate=1778774400000, pubDateStr=2026-05-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789098321822, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789098321822, creator=13701087609, updateTime=1789098321822, updator=13701087609, issue=Issue{id=1305136200847740945, tenantId=1146029695717560320, journalId=1303687221787852879, year='2026', volume='46', issue='5', pageStart='F2', pageEnd='706', issueExtLink='null', onlineDate='null', pubDate='1778774400000', pubDateStr='2026-05-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789098318540, creator='13701087609', updateTime=1789103109806, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305156296924222402, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136200847740945, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305156296924222403, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136200847740945, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=567, endPage=575, ext={EN=ArticleExt(id=1305136214839944157, articleId=1305136214613451740, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Analysis of Structural Variations of 1RS in Wheat-Rye 1BL·1RS Translocation Cultivars(Lines), columnId=1305133119129023428, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

To explore the structural differences of 1RS arms in wheat-rye 1BL·1RS translocation cultivars (lines), the 1BL·1RS translocation cultivar Nanmai 618 was used as parent to hybridize with 13 1BL·1RS translocation cultivars (lines), including Aikang 58, Chuannong 17 and Lankao Aizao 8, as well as the 7DL·1RS translocation cultivar Lumai 8. A total of 14 types of F1 plants were obtained. High-GC content oligonucleotide probes (Oligo-119.2-2, 200G, 250G) and high-AT content oligonucleotide probes (Oligo-119.2-3, 200T, 250T) were designed based on the tandem repeats pSc119.2, pSc200, and pSc250. These probes were used in non-denaturing fluorescence in situ hybridization (ND-FISH) analysis for identifying the metaphase chromosomes of root tips from these F1 plants. The results showed that, except for Oligo-119.2-3 which produced no signal, the other five probes generated signals with varying intensities on 1RS chromosome arms from different sources. The signal intensities of probes 200G and 200T on 1RS exhibited consistent variation trends, while the variations reflected by 250G and 250T were inconsistent. For example, 250T showed distinct signal intensities on 1RS from different sources, whereas 250G displayed similar signal intensities on the corresponding 1RS arms. These findings indicated that there were differences in the GC and AT base contents of the pSc250 tandem repeated family in 1RS among different cultivars. According to the variation in signal intensity of the probes on 1RS, the 1RS arms in these 15 wheat cultivars (lines) could be classified into four structural types. The results of this study revealed the structural variations of 1RS, which enriches the understanding of 1BL·1RS translocation chromosomes in wheat cultivars (lines) in China.

, authors=Jingqi GUO1, 2, Ziying XIONG1, Shulan FU1, Huaiyu ZHANG2, Zongxiang TANG1, authorsList=Jingqi GUO, Ziying XIONG, Shulan FU, Huaiyu ZHANG, Zongxiang TANG, authorCompany=null, correspAuthors=Huaiyu ZHANG, Zongxiang TANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305136215079019486, articleId=1305136214613451740, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=小麦-黑麦1BL·1RS易位品种(系)中的1RS结构变异探析, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

为了探究小麦-黑麦1BL·1RS易位品种(系)中的1RS结构差异,以1BL·1RS易位品种南麦618为亲本,分别与矮抗58、川农17、兰考矮早8等13个1BL·1RS易位品种(系)和1个7DL·1RS易位品种鲁麦8号杂交,获得14组F1植株。使用根据串联重复序列pSc119.2、pSc200和pSc250设计的高GC含量寡核苷酸探针Oligo-119.2-2、200G、250G和高AT含量寡核苷酸探针Oligo-119.2-3、200T、250T,对F1植株根尖中期染色体进行ND-FISH分析。结果表明,除Oligo-119.2-3无信号外,其余5个探针都在不同来源的1RS上产生不同强弱信号。探针200G和200T在1RS上产生的信号呈现一致的强弱变化,而250G和250T体现的变异不一致。250T在不同来源1RS上的信号强弱不同,而250G在相应1RS上的信号强度相似。这说明不同品种中1RS的pSc250家族串联重复序列的GC和AT碱基含量存在差异。根据探针在1RS上的信号强弱变化,可以将这15个小麦品种(系)中的1RS分为4种结构类型。本研究结果显示了1RS的结构多样性,丰富了对中国小麦品种(系)中1BL·1RS易位染色体的认识。

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小麦-黑麦1BL·1RS易位品种(系)中的1RS结构变异探析
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郭婧琦 1, 2 , 熊自颖 1 , 符书兰 1 , 张怀渝 2 , 唐宗祥 1
麦类作物学报 | 遗传育种 2026,46(5): 567-575
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麦类作物学报 |遗传育种 2026 , 46 (5) : 567 -575
小麦-黑麦1BL·1RS易位品种(系)中的1RS结构变异探析
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郭婧琦1, 2 , 熊自颖1 , 符书兰1, 张怀渝2 , 唐宗祥1
作者信息
  • 1.四川农业大学农学院,四川 成都 611130
  • 2.四川农业大学生命科学学院,四川 雅安 625000
通讯作者:
E-mail: (唐宗祥)
(张怀渝)
作者简介:

E-mail: (郭婧琦)

(熊自颖)

Analysis of Structural Variations of 1RS in Wheat-Rye 1BL·1RS Translocation Cultivars(Lines)
Jingqi GUO1, 2 , Ziying XIONG1 , Shulan FU1, Huaiyu ZHANG2 , Zongxiang TANG1
Affiliations
  • 1.College of Agriculture, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
  • 2.College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan 625000, China
出版时间: 2026-05-15 doi: 10.7606/j.issn.1009-1041.2026.05.01
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为了探究小麦-黑麦1BL·1RS易位品种(系)中的1RS结构差异,以1BL·1RS易位品种南麦618为亲本,分别与矮抗58、川农17、兰考矮早8等13个1BL·1RS易位品种(系)和1个7DL·1RS易位品种鲁麦8号杂交,获得14组F1植株。使用根据串联重复序列pSc119.2、pSc200和pSc250设计的高GC含量寡核苷酸探针Oligo-119.2-2、200G、250G和高AT含量寡核苷酸探针Oligo-119.2-3、200T、250T,对F1植株根尖中期染色体进行ND-FISH分析。结果表明,除Oligo-119.2-3无信号外,其余5个探针都在不同来源的1RS上产生不同强弱信号。探针200G和200T在1RS上产生的信号呈现一致的强弱变化,而250G和250T体现的变异不一致。250T在不同来源1RS上的信号强弱不同,而250G在相应1RS上的信号强度相似。这说明不同品种中1RS的pSc250家族串联重复序列的GC和AT碱基含量存在差异。根据探针在1RS上的信号强弱变化,可以将这15个小麦品种(系)中的1RS分为4种结构类型。本研究结果显示了1RS的结构多样性,丰富了对中国小麦品种(系)中1BL·1RS易位染色体的认识。

小麦  /  黑麦  /  1BL·1RS易位  /  串联重复序列  /  染色体结构变异

To explore the structural differences of 1RS arms in wheat-rye 1BL·1RS translocation cultivars (lines), the 1BL·1RS translocation cultivar Nanmai 618 was used as parent to hybridize with 13 1BL·1RS translocation cultivars (lines), including Aikang 58, Chuannong 17 and Lankao Aizao 8, as well as the 7DL·1RS translocation cultivar Lumai 8. A total of 14 types of F1 plants were obtained. High-GC content oligonucleotide probes (Oligo-119.2-2, 200G, 250G) and high-AT content oligonucleotide probes (Oligo-119.2-3, 200T, 250T) were designed based on the tandem repeats pSc119.2, pSc200, and pSc250. These probes were used in non-denaturing fluorescence in situ hybridization (ND-FISH) analysis for identifying the metaphase chromosomes of root tips from these F1 plants. The results showed that, except for Oligo-119.2-3 which produced no signal, the other five probes generated signals with varying intensities on 1RS chromosome arms from different sources. The signal intensities of probes 200G and 200T on 1RS exhibited consistent variation trends, while the variations reflected by 250G and 250T were inconsistent. For example, 250T showed distinct signal intensities on 1RS from different sources, whereas 250G displayed similar signal intensities on the corresponding 1RS arms. These findings indicated that there were differences in the GC and AT base contents of the pSc250 tandem repeated family in 1RS among different cultivars. According to the variation in signal intensity of the probes on 1RS, the 1RS arms in these 15 wheat cultivars (lines) could be classified into four structural types. The results of this study revealed the structural variations of 1RS, which enriches the understanding of 1BL·1RS translocation chromosomes in wheat cultivars (lines) in China.

Wheat  /  Rye  /  1BL·1RS translocation  /  Tandem repeats  /  Chromosome structural variation
郭婧琦, 熊自颖, 符书兰, 张怀渝, 唐宗祥. 小麦-黑麦1BL·1RS易位品种(系)中的1RS结构变异探析. 麦类作物学报, 2026 , 46 (5) : 567 -575 . DOI: 10.7606/j.issn.1009-1041.2026.05.01
Jingqi GUO, Ziying XIONG, Shulan FU, Huaiyu ZHANG, Zongxiang TANG. Analysis of Structural Variations of 1RS in Wheat-Rye 1BL·1RS Translocation Cultivars(Lines)[J]. Journal of Triticeae Crops, 2026 , 46 (5) : 567 -575 . DOI: 10.7606/j.issn.1009-1041.2026.05.01
  • 国家自然科学基金项目(32070373)
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doi: 10.7606/j.issn.1009-1041.2026.05.01
  • 接收时间:2025-09-25
  • 首发时间:2026-09-11
  • 出版时间:2026-05-15
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  • 收稿日期:2025-09-25
  • 修回日期:2026-01-19
基金
国家自然科学基金项目(32070373)
作者信息
    1.四川农业大学农学院,四川 成都 611130
    2.四川农业大学生命科学学院,四川 雅安 625000

通讯作者:

E-mail: (唐宗祥)
(张怀渝)
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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