Article(id=1307627333297393831, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627319351333857, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.10.04, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1740326400000, receivedDateStr=2025-02-24, revisedDate=1747497600000, revisedDateStr=2025-05-18, acceptedDate=null, acceptedDateStr=null, onlineDate=1789692250799, onlineDateStr=2026-09-18, pubDate=1760457600000, pubDateStr=2025-10-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789692250799, onlineIssueDateStr=2026-09-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789692250799, creator=13701087609, updateTime=1789692250799, updator=13701087609, issue=Issue{id=1307627319351333857, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='10', pageStart='F2', pageEnd='1440', issueExtLink='null', onlineDate='null', pubDate='1760457600000', pubDateStr='2025-10-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789692247474, creator='13701087609', updateTime=1789722059268, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1307752359971545845, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627319351333857, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1307752359971545846, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627319351333857, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1324, endPage=1330, ext={EN=ArticleExt(id=1307627333578412200, articleId=1307627333297393831, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Characterization and Activity Identification of Thioredoxin TaTrxh10 in Wheat, columnId=1307627250845770215, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breedin, runingTitle=null, highlight=null, articleAbstract=

Thioredoxins are a class of redox regulatory proteins widely present in plants, which play crucial roles in plant growth and development. To investigate the biological functions of the wheat thioredoxin TaTrxh10, we systematically analyzed its sequence characteristics, three-dimensional structure, and gene expression patterns. The protein was expressed in a prokaryotic system, and its disulfide bond reduction activity was validated in vitro. Sequence analysis revealed that wheat TaTrxh10 comprises three family members encoded by three homoeologous genes: TaTrxh10-A, TaTrxh10-B, and TaTrxh10-D. All three TaTrxh10 family members consist of 143 amino acids, with a sequence identity of 94.4%, and contain the classic thioredoxin conserved domain and catalytic active center (WCGPC). Protein structural analysis demonstrated that TaTrxh10 can fold into a sandwich-like spatial structure composed of five β-sheets and four α-helices. Gene expression analysis indicated that the three homoeologous genes of TaTrxh10 are expressed at very low levels in roots, stems, and leaves, and at relatively lower levels in the ovary and mature seeds; however, they are highly expressed in mature pollens. The thioredoxin reduction activity assay using DTNB demonstrated that all three family members of TaTrxh10 possess the ability to catalyze disulfide bond reduction, but their reduction activities varied among the members. In reaction systems with lower thioredoxin concentrations, TaTrxh10-D exhibited the highest catalytic activity, followed by TaTrxh10-B. These results indicate that wheat TaTrxh10 represents a class of active redox regulatory proteins specifically expressed in pollen, which provides important insights for further exploration of redox-dependent developmental regulatory mechanisms in wheat.

, authors=Zhongfa JIAO1, Xiaoteng WANG1, Shuaijing DING1, Yujie WU1, Shuhan JING1, Li ZHANG1, Xiaodan MENG1, Fanrong MENG2, Yongchun LI1, authorsList=Zhongfa JIAO, Xiaoteng WANG, Shuaijing DING, Yujie WU, Shuhan JING, Li ZHANG, Xiaodan MENG, Fanrong MENG, Yongchun LI, authorCompany=null, correspAuthors=Yongchun LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307627333901373609, articleId=1307627333297393831, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=小麦硫氧还蛋白TaTrxh10的特征及活性鉴定, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

硫氧还蛋白是一类在植物中普遍存在的氧化还原调控蛋白,在植物生长发育过程中发挥重要调控功能。为了探讨小麦硫氧还蛋白TaTrxh10的生物学功能,系统分析了该蛋白的序列特征、三维结构及其基因表达模式,并将该蛋白原核表达后进行了其对二硫键还原活性的体外鉴定。蛋白序列特征分析显示,小麦TaTrxh10包含3个成员,分别由3个同源基因TaTrxh10-A、TaTrxh10-B和TaTrxh10-D编码;3个成员均包含143个氨基酸,序列一致性为94.4%,且具有经典的硫氧还蛋白保守结构域和催化活性中心(WCGPC)。蛋白高级结构分析显示,TaTrxh10可形成由5个β-折叠和4个α-螺旋组成的三明治型特定空间结构。基因表达特性分析发现,TaTrxh10的3个同源基因在小麦根、茎和叶中的表达量极低,在子房和成熟种子中有较低水平的表达,而在成熟花粉中高表达。利用DTNB进行的硫氧还蛋白还原活性鉴定表明,TaTrxh10的3个成员均具有催化二硫键还原的功能,不过其还原活性在3个成员间存在一定差异;在较低浓度硫氧还蛋白的反应体系中,TaTrxh10-D的催化活性最高,TaTrxh10-B次之。以上结果表明,小麦TaTrxh10是一类在花粉中特异表达的有活性的氧化还原调控蛋白,这为进一步探讨小麦中依赖于氧化还原的发育调控机制提供了重要信息。

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小麦硫氧还蛋白TaTrxh10的特征及活性鉴定
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焦中发 1 , 王晓腾 1 , 丁帅静 1 , 吴玉杰 1 , 景姝涵 1 , 张莉 1 , 孟晓丹 1 , 孟凡荣 2 , 李永春 1
麦类作物学报 | 遗传育种 2025,45(10): 1324-1330
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麦类作物学报 |遗传育种 2025 , 45 (10) : 1324 -1330
小麦硫氧还蛋白TaTrxh10的特征及活性鉴定
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焦中发1 , 王晓腾1, 丁帅静1, 吴玉杰1, 景姝涵1, 张莉1, 孟晓丹1, 孟凡荣2, 李永春1
作者信息
  • 1.河南农业大学农学院/国家小麦工程技术研究中心,河南 郑州 450046
  • 2.河南农业大学生命科学学院,河南 郑州 450046
通讯作者:
E-mail: (李永春)
作者简介:

E-mail: (焦中发)

Characterization and Activity Identification of Thioredoxin TaTrxh10 in Wheat
Zhongfa JIAO1 , Xiaoteng WANG1, Shuaijing DING1, Yujie WU1, Shuhan JING1, Li ZHANG1, Xiaodan MENG1, Fanrong MENG2, Yongchun LI1
Affiliations
  • 1.College of Agronomy/National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, Henan 450046, China
  • 2.College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan 450046, China
出版时间: 2025-10-15 doi: 10.7606/j.issn.1009-1041.2025.10.04
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硫氧还蛋白是一类在植物中普遍存在的氧化还原调控蛋白,在植物生长发育过程中发挥重要调控功能。为了探讨小麦硫氧还蛋白TaTrxh10的生物学功能,系统分析了该蛋白的序列特征、三维结构及其基因表达模式,并将该蛋白原核表达后进行了其对二硫键还原活性的体外鉴定。蛋白序列特征分析显示,小麦TaTrxh10包含3个成员,分别由3个同源基因TaTrxh10-A、TaTrxh10-B和TaTrxh10-D编码;3个成员均包含143个氨基酸,序列一致性为94.4%,且具有经典的硫氧还蛋白保守结构域和催化活性中心(WCGPC)。蛋白高级结构分析显示,TaTrxh10可形成由5个β-折叠和4个α-螺旋组成的三明治型特定空间结构。基因表达特性分析发现,TaTrxh10的3个同源基因在小麦根、茎和叶中的表达量极低,在子房和成熟种子中有较低水平的表达,而在成熟花粉中高表达。利用DTNB进行的硫氧还蛋白还原活性鉴定表明,TaTrxh10的3个成员均具有催化二硫键还原的功能,不过其还原活性在3个成员间存在一定差异;在较低浓度硫氧还蛋白的反应体系中,TaTrxh10-D的催化活性最高,TaTrxh10-B次之。以上结果表明,小麦TaTrxh10是一类在花粉中特异表达的有活性的氧化还原调控蛋白,这为进一步探讨小麦中依赖于氧化还原的发育调控机制提供了重要信息。

小麦  /  硫氧还蛋白  /  序列特征  /  原核表达  /  还原活性

Thioredoxins are a class of redox regulatory proteins widely present in plants, which play crucial roles in plant growth and development. To investigate the biological functions of the wheat thioredoxin TaTrxh10, we systematically analyzed its sequence characteristics, three-dimensional structure, and gene expression patterns. The protein was expressed in a prokaryotic system, and its disulfide bond reduction activity was validated in vitro. Sequence analysis revealed that wheat TaTrxh10 comprises three family members encoded by three homoeologous genes: TaTrxh10-A, TaTrxh10-B, and TaTrxh10-D. All three TaTrxh10 family members consist of 143 amino acids, with a sequence identity of 94.4%, and contain the classic thioredoxin conserved domain and catalytic active center (WCGPC). Protein structural analysis demonstrated that TaTrxh10 can fold into a sandwich-like spatial structure composed of five β-sheets and four α-helices. Gene expression analysis indicated that the three homoeologous genes of TaTrxh10 are expressed at very low levels in roots, stems, and leaves, and at relatively lower levels in the ovary and mature seeds; however, they are highly expressed in mature pollens. The thioredoxin reduction activity assay using DTNB demonstrated that all three family members of TaTrxh10 possess the ability to catalyze disulfide bond reduction, but their reduction activities varied among the members. In reaction systems with lower thioredoxin concentrations, TaTrxh10-D exhibited the highest catalytic activity, followed by TaTrxh10-B. These results indicate that wheat TaTrxh10 represents a class of active redox regulatory proteins specifically expressed in pollen, which provides important insights for further exploration of redox-dependent developmental regulatory mechanisms in wheat.

Wheat  /  Thioredoxin  /  Sequence characterization  /  Prokaryotic expression  /  Reduction activity
焦中发, 王晓腾, 丁帅静, 吴玉杰, 景姝涵, 张莉, 孟晓丹, 孟凡荣, 李永春. 小麦硫氧还蛋白TaTrxh10的特征及活性鉴定. 麦类作物学报, 2025 , 45 (10) : 1324 -1330 . DOI: 10.7606/j.issn.1009-1041.2025.10.04
Zhongfa JIAO, Xiaoteng WANG, Shuaijing DING, Yujie WU, Shuhan JING, Li ZHANG, Xiaodan MENG, Fanrong MENG, Yongchun LI. Characterization and Activity Identification of Thioredoxin TaTrxh10 in Wheat[J]. Journal of Triticeae Crops, 2025 , 45 (10) : 1324 -1330 . DOI: 10.7606/j.issn.1009-1041.2025.10.04
  • 河南省重大科技专项(241100110300)
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doi: 10.7606/j.issn.1009-1041.2025.10.04
  • 接收时间:2025-02-24
  • 首发时间:2026-09-18
  • 出版时间:2025-10-15
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  • 收稿日期:2025-02-24
  • 修回日期:2025-05-18
基金
河南省重大科技专项(241100110300)
作者信息
    1.河南农业大学农学院/国家小麦工程技术研究中心,河南 郑州 450046
    2.河南农业大学生命科学学院,河南 郑州 450046

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E-mail: (李永春)
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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