Article(id=1307627835825344965, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627835330417093, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.08.01, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1723564800000, receivedDateStr=2024-08-14, revisedDate=1736092800000, revisedDateStr=2025-01-06, acceptedDate=null, acceptedDateStr=null, onlineDate=1789692370611, onlineDateStr=2026-09-18, pubDate=1755187200000, pubDateStr=2025-08-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789692370611, onlineIssueDateStr=2026-09-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789692370611, creator=13701087609, updateTime=1789692370611, updator=13701087609, issue=Issue{id=1307627835330417093, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='8', pageStart='F2', pageEnd='1146', issueExtLink='null', onlineDate='null', pubDate='1755187200000', pubDateStr='2025-08-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789692370493, creator='13701087609', updateTime=1789723574163, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1307758714606346994, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627835330417093, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1307758714610541299, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1307627835330417093, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1003, endPage=1010, ext={EN=ArticleExt(id=1307627836009894342, articleId=1307627835825344965, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Creation of Chromosome Translocation Lines between Wheat and Aegilops uniaristata, columnId=1307627250845770215, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breedin, runingTitle=null, highlight=null, articleAbstract=

The 1N chromosome of Aegilops uniaristata contains superior genes essential for wheat breeding. To rapidly and accurately identify the 1N chromosome of Aegilops uniaristata from a wheat background, this study utilized 212 primer pairs to analyze Aegilops uniaristata, a set of wheat-Aegilops uniaristata addition lines, with a wheat material as control. The results showed that after digestion with Taq Ⅰ enzyme, the amplification products of primers TNAC1006, TNAC1017, TNAC1029, and TANC1031 yielded polymorphic fragments of 900, 1200, 1800, and 400 bp in Aegilops uniaristata and the wheat-Aegilops uniaristata 1N addition lines, respectively. These polymorphic fragments were not observed in other tested materials, and thus they can serve as specific molecular markers for detecting 1N chromosome in a wheat background. Using these established molecular markers, the wheat-Aegilops uniaristata 1N addition lines and the F₂ population of Chinese Spring hybrids were screened. Simultaneously, dual-color FISH with Oligo-pSc119.2-1 and Oligo-pTa535-1 as probes was used to identify the corresponding individual plants. It was found that plants N91 and N350 contain heterozygous 1NS·1NS isochromosomes; N46 contains a heterozygous 1N+1NL·1NL chromosome; and N319 contains a heterozygous 1BS·1NS translocation chromosome. Further molecular marker and FISH analysis of the 207 self-pollinated progeny from these four plants yielded only one homozygous wheat-Aegilops uniaristata 1NS·1NS translocation line. To investigate the reason for the extremely low proportion of homozygous translocation lines obtained, a test cross was conducted using the 1BS·1NS+1B heterozygous line and Jimai 38 as materials. The results confirmed that 1BS·1NS is transmitted only through female gametes.

, authors=Wenping GONG, Xiaolu WANG, Ran HAN, Aifeng LIU, Haosheng LI, Jianjun LIU, Wenjing XU, Cheng LIU, authorsList=Wenping GONG, Xiaolu WANG, Ran HAN, Aifeng LIU, Haosheng LI, Jianjun LIU, Wenjing XU, Cheng LIU, authorCompany=null, correspAuthors=Wenjing XU, Cheng LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307627836433519047, articleId=1307627835825344965, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=小麦-单芒山羊草染色体易位系创制, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

单芒山羊草1N染色体上含有小麦育种所需的优异基因，为快速准确鉴定小麦背景中的单芒山羊草1N染色质，本研究利用合成的212对引物对单芒山羊草、一套小麦-单芒山羊草附加系和小麦对照进行分析。结果表明，引物TNAC1006、TNAC1017、TNAC1029和TANC1031的扩增产物经Taq Ⅰ酶切后，在单芒山羊草和小麦-单芒山羊草1N附加系中分别获得长度为900、1 200、1 800和400 bp的多态性片段，而在其他供试材料中未获得这些多态性片段，因此它们可作为特异分子标记用于小麦背景中的1N染色质检测。利用建立分子标记筛选小麦-单芒山羊草1N附加系和中国春杂交F₂群体，同时用以Oligo-pSc119.2-1和Oligo-pTa535-1为探针的双色FISH鉴定相应单株，结果发现，单株N91和N350含有杂合1NS·1NS等臂染色体，N46含有杂合1N+1NL·1NL染色体，N319含有杂合1BS·1NS易位染色体。进一步对上述4个单株的207个自交后代单株进行分子标记和FISH鉴定，结果仅获得1份小麦-单芒山羊草1NS·1NS纯合易位系。为研究清楚获得纯合易位系比例极低的原因，以1BS·1NS+1B杂合系与济麦38为材料进行测交，结果证实1BS·1NS仅通过雌配子传递。

, authors=宫文萍, 汪晓潞, 韩冉, 刘爱峰, 李豪圣, 刘建军, 徐文竞, 刘成, authorsList=宫文萍, 汪晓潞, 韩冉, 刘爱峰, 李豪圣, 刘建军, 徐文竞, 刘成, authorCompany=null, correspAuthors=徐文竞, 刘成, authorNote=, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=yzTnLA6xNEsT8elpD1h/mg==, magXml=1dRJCHlc24VvBdhtIwE1VQ==, pdfUrl=null, pdf=E53XJdvvlbdnDNEGOXFH1w==, pdfFileSize=1118104, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=V3/vwmKrK8h9zn2/kNqP2A==, mapNumber=null, fund=null)}, authors=[Author(id=1307627911817748997, tenantId=1146029695717560320, journalId=1303687221787852879, articleId=1307627835825344965, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=gongwenping911@126.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307627911889052167, tenantId=1146029695717560320, journalId=1303687221787852879, articleId=1307627835825344965, authorId=1307627911817748997, language=EN, stringName=Wenping GONG, firstName=Wenping, middleName=null, lastName=GONG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Crop Research Institute, Shandong Academy of Agricultural Sciences/National Engineering Research Center of Wheat and Maize, National Key Laboratory of Wheat Breeding/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley, Ministry of Agriculture/Shandong Wheat Technology Innovation Center, Jinan, Shandong 250100, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307627911960355336, tenantId=1146029695717560320, journalId=1303687221787852879, articleId=1307627835825344965, authorId=1307627911817748997, language=CN, stringName=宫文萍, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=山东省农业科学院作物研究所/小麦玉米国家工程研究中心/小麦育种全国重点实验室/农业部黄淮北部小麦生物学与遗传育种重点实验室/山东省小麦技术创新中心，山东 济南 250100, bio={"content":"

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