Article(id=1305136130832228726, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136112096272647, articleNumber=null, orderNo=null, doi=10.7606/j.issn.1009-1041.2025.04.02, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1710691200000, receivedDateStr=2024-03-18, revisedDate=1713801600000, revisedDateStr=2024-04-23, acceptedDate=null, acceptedDateStr=null, onlineDate=1789098301846, onlineDateStr=2026-09-11, pubDate=1744646400000, pubDateStr=2025-04-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789098301846, onlineIssueDateStr=2026-09-11, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789098301846, creator=13701087609, updateTime=1789098301846, updator=13701087609, issue=Issue{id=1305136112096272647, tenantId=1146029695717560320, journalId=1303687221787852879, year='2025', volume='45', issue='4', pageStart='F2', pageEnd='562', issueExtLink='null', onlineDate='null', pubDate='1744646400000', pubDateStr='2025-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789098297379, creator='13701087609', updateTime=1789103273005, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1305156981438832926, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136112096272647, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1305156981438832927, tenantId=1146029695717560320, journalId=1303687221787852879, issueId=1305136112096272647, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=432, endPage=439, ext={EN=ArticleExt(id=1305136131918553463, articleId=1305136130832228726, tenantId=1146029695717560320, journalId=1303687221787852879, language=EN, title=Screening of Culture Medium and Concentration of Colchicine for Construction of Wheat DH Lines and Their HMW-GS Composition Analysis, columnId=1305133119129023428, journalTitle=Journal of Triticeae Crops, columnName=Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

In order to improve the construction of wheat DH lines, the F₁ generation of 11 wheat hybrid combinations was used as materials to compare and analyze the induction efficiency of wheat anthers under two media (NPB-99 and CHB) and the chromosome doubling efficiency of haploids under three concentrations of colchicine treatments (4, 6 and 8 g·L^-1). The high-molecular-weight glutenin subunits (HMW-GS) of the obtained DH lines were identified by SDS-PAGE. The results showed that the anther induction efficiency of CHB medium was better than that of NPB-99 medium. The concentration of 6 g·L^-1 colchicine was more efficient in inducing haploid doubling. A total of 16 lines carrying prior HMW-GS combinations (1/7+8/5+10 or 1/7+9/5+10) were identified from the 33 DH lines, with good baking quality, which could provide genetic resources for subsequent wheat quality breeding research.

, authors=Zhenzhen BI¹, Yan ZHENG¹, Hui LI¹, Xiaojian LI¹, Wen LIU¹, Xuerong MU¹, Na ZHU¹, Jiale ZHENG¹, Yulong SONG¹, Donghong MIN¹, Xiaohong ZHANG², authorsList=Zhenzhen BI, Yan ZHENG, Hui LI, Xiaojian LI, Wen LIU, Xuerong MU, Na ZHU, Jiale ZHENG, Yulong SONG, Donghong MIN, Xiaohong ZHANG, authorCompany=null, correspAuthors=Donghong MIN, Xiaohong ZHANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1305136134149923192, articleId=1305136130832228726, tenantId=1146029695717560320, journalId=1303687221787852879, language=CN, title=小麦DH系创制中培养基、秋水仙浓度的筛选及其HMW-GS组成分析, columnId=1305133119284212678, journalTitle=麦类作物学报, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=

为了提高小麦DH系创制效率，以11个小麦杂交组合的F₁代为材料，比较分析了两种培养基（NPB-99和CHB）下小麦花药的诱导效率以及三种浓度秋水仙素处理（4、6和8 g·L^-1）下单倍体的染色体加倍效率，并利用SDS-PAGE对获得的DH株系进行优质亚基检测。结果表明，CHB培养基花药诱导效率优于NPB-99培养基。在三种秋水仙素浓度中，6 g·L^-1诱导单倍体加倍的效率最高。在所获得的33个DH系中共鉴定出16个株系携带优质亚基组合（1/7+8/5+10或1/7+9/5+10），且具有较好的烘焙品质，可作为小麦品质育种的种质资源。

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