Article(id=1322559877113823420, tenantId=1146029695717560320, journalId=1225396423026438145, issueId=1322559853093048851, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1740672000000, receivedDateStr=2025-02-28, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1793252446677, onlineDateStr=2026-10-29, pubDate=1771948800000, pubDateStr=2026-02-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793252446677, onlineIssueDateStr=2026-10-29, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793252446677, creator=13701087609, updateTime=1793252446677, updator=13701087609, issue=Issue{id=1322559853093048851, tenantId=1146029695717560320, journalId=1225396423026438145, year='2026', volume='46', issue='2', pageStart='1', pageEnd='326', issueExtLink='null', onlineDate='null', pubDate='1771948800000', pubDateStr='2026-02-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1793252440951, creator='13701087609', updateTime=1793252699964, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322560939526828556, tenantId=1146029695717560320, journalId=1225396423026438145, issueId=1322559853093048851, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322560939526828557, tenantId=1146029695717560320, journalId=1225396423026438145, issueId=1322559853093048851, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=239, endPage=250, ext={EN=ArticleExt(id=1322559877294178493, articleId=1322559877113823420, tenantId=1146029695717560320, journalId=1225396423026438145, language=EN, title=Optimization of Industrial-Grade Culture Medium Ratio in Microbial Mineralization Based on Response Surface Methodology, columnId=null, journalTitle=Mining Research and Development, columnName=null, runingTitle=null, highlight=null, articleAbstract=

In order to reduce the cost of microbially induced carbonate precipitation (MICP), the effects of industrial-grade bovine bone protein peptone dosages, soya peptone dosages, sodium chloride dosages and urea dosages on the microbial quantity OD₆₀₀ value and urease activity of Sporosarcina pasteurii cultured for 16 hours were studied. Response surface methodology was used to optimize the optimal ratio of industrial culture medium. And the microbial quantity OD₆₀₀ value and urease activity of the bacterial solution under the optimal ratio were detected. After the bacterial solution cultured for 16 hours reacting with different concentrations of cementing solution, the amount of calcium carbonate produced by the two reactions was measured, and the calcium carbonate crystals was analyzed using scanning electron microscopy (SEM). The results show that the optimal medium ratio is 25 g/L of bovine bone protein peptone, 7.5 g/L of soya peptone, 5 g/L of industrial sodium chloride, 22.2 g/L of industrial urea, and 1 000 mL of pure water. The OD₆₀₀ value of Sporosarcina pasteurii liquid cultured for 16 hours under this ratio is 1.672, and the urease activity is 0.037 mmol/(L·min), both of which are within the prediction error, indicating feasibility of the results. The content of calcium carbonate generated by cementation under the two media is not much different, and the cementation effect of industrial-grade culture medium bacterial solution is stronger than that of analytical pure culture medium bacterial solution. The research results can provide a low-cost medium ratio scheme for the large-scale engineering application of MICP.

, authors=Zhijun ZHANG^{1, 2}, Hao BAI^{1, 2}, Meizhong HUANG³, Huaimiao ZHENG^{1, 2}, Yakun TIAN^{1, 2}, Lin HU^{1, 2}, Lingling WU^{1, 2}, authorsList=Zhijun ZHANG, Hao BAI, Meizhong HUANG, Huaimiao ZHENG, Yakun TIAN, Lin HU, Lingling WU, authorCompany=null, correspAuthors=Lingling WU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322559877541642430, articleId=1322559877113823420, tenantId=1146029695717560320, journalId=1225396423026438145, language=CN, title=基于响应曲面法的微生物矿化工业级培养基配比优选, columnId=1226462295417598973, journalTitle=矿业研究与开发, columnName=矿山安全与环保, runingTitle=null, highlight=null, articleAbstract=

为了降低微生物诱导碳酸钙沉淀（MICP）的成本，研究了工业级的牛骨蛋白胨掺量、大豆蛋白胨掺量、氯化钠掺量、尿素掺量对培养16 h的巴氏芽孢杆菌微生物OD₆₀₀值和脲酶活性的影响。利用响应曲面法优选工业级培养基的最佳配比。对最佳配比下的菌液进行微生物OD₆₀₀值和脲酶活性检测。将培养16 h的菌液与不同浓度的胶结液反应，对两者反应的碳酸钙生成量进行测定，并对碳酸钙晶体进行扫描电子显微镜（SEM）分析。结果表明：最佳配比为牛骨蛋白胨25 g/L、大豆蛋白胨7.5 g/L、工业氯化钠5 g/L、工业尿素22.2 g/L及纯水1 000 mL。该配比下培养16 h的巴氏芽孢杆菌菌液的OD₆₀₀值为1.672，脲酶活性为0.037 mmol/（L·min），均在预测值误差之内，结果具有可行性。两种培养基下的胶结生成的碳酸钙量相差不大，且工业级培养基菌液的胶结效果强于分析纯培养基菌液。研究结果可为MICP的工程大范围应用提供一个成本较低的培养基配比方案。

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张志军（1978—），男，河北邢台人，博士，教授，主要研究方向为矿山岩土灾害预测与控制。E-mail:zzj181@163.com

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张志军（1978—），男，河北邢台人，博士，教授，主要研究方向为矿山岩土灾害预测与控制。E-mail:zzj181@163.com

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