Article(id=1250863269171376599, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242131072943723237, articleNumber=null, orderNo=null, doi=10.3981/j.issn.1000-7857.2013.32.010, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1376323200000, receivedDateStr=2013-08-13, revisedDate=1379952000000, revisedDateStr=2013-09-24, acceptedDate=null, acceptedDateStr=null, onlineDate=1384704000000, onlineDateStr=2013-11-18, pubDate=1384704000000, pubDateStr=2013-11-18, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1384704000000, onlineIssueDateStr=2013-11-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1776158643283, creator=sys-migrate, updateTime=1776158643283, updator=sys-migrate, issue=Issue{id=1242131072943723237, tenantId=1146029695717560320, journalId=1146031591421210625, year='2013', volume='31', issue='32', pageStart='3', pageEnd='96', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774076725467, creator=sys-migrate, updateTime=1774076725467, updator=sys-migrate, preIssue=null, nextIssue=null, ext=null, issueFiles=null}, startPage=64, endPage=67, ext={EN=ArticleExt(id=1250863275794186601, articleId=1250863269171376599, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Influence of LIPUS on Proliferation, Differentiation and Mineralization of MC3T3-E1 Cells, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=The aim of the present study is to investigate the effectiveness of low-intensity pulsed ultrasound(LIPUS) on cell proliferation, differentiation and mineralizationusing of MC3T3-E1 cell line. The MC3T3-E1 cells were exposed to LIPUS with different parameters(frenquency and intensity). The cell proliferations, differentiation, and mineralization were determined by CCK-8 method, alkaline phosphatase detection kit, and alizarin red staining, respectively. The ultrasound intensity of 30mW/cm² and 40mW/cm² could promote cell proliferation, while 50mW/cm² inhibit it. The lower intensity group under 1.5MHz and 1.7MHz present better alkaline phosphatase activity. The 1.5MHz and 40 mW/cm² groups showed better mineralization effect than others. LIPUS can promote MC3T3-E1 on cell proliferation, differentiation and mineralization. LIPUS may act as a new method forprevention and treatment of osteoporosis., correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=777plDtaaWtdxT2wPSaWBA==, pdfFileSize=832319, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. Department of Biopharmaceuticals, Beijing University of Chinese Medicine, Beijing 100102, China;2. Department of Engineering Physics, Tsinghua University, Beijing 100084, China;3. Department of Laboratory Animal Quality Testing, National Institutes for Food and Drug Control, Beijing 100050, China, fund=null, authors=WANG Ran¹, MA Jun¹, ZHAN Xin¹, XIN Min¹, WANG Jie², WANG Xiaowen², TANG Jintian², YUE Bingfei^1,3, authorsList=WANG Ran;MA Jun;ZHAN Xin;XIN Min;WANG Jie;WANG Xiaowen;TANG Jintian;YUE Bingfei;), CN=ArticleExt(id=1250863273889968612, articleId=1250863269171376599, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=低强度脉冲超声对MC3T3-E1细胞增殖、分化和矿化的影响, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为了研究低强度脉冲超声促进MC3T3-E1细胞增殖、分化和矿化的有效性,初步筛选频率和强度参数,分别对细胞施加不同参数(频率、强度)的超声,采取CCK-8法检测细胞的增殖情况,碱性磷酸酶检测试剂盒检测分化效果,茜素红染色观察矿化效果。发现30mW/cm²和40mW/cm²的超声对细胞产生促增殖作用,50mW/cm²抑制增殖;1.5MHz和1.7MHz的较低强度组均较对照组的ALP活性有增加;1.5MHz,40mW/cm²的超声组矿化效果较其他组好。研究结果表明,低强度的脉冲超声可以提高成骨细胞株MC3T3-E1增殖、分化和矿化能力,可能是防治骨质疏松的可行手段之一。, correspAuthors=null, authorNote=null, correspAuthorsNote=岳秉飞,研究员,研究方向为比较医学,电子信箱:y6784@126.com, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=Xenjq3ilbUsu6zEp4XdAsA==, pdfFileSize=832319, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. 北京中医药大学生物制药系, 北京 100102;2. 清华大学工程物理系, 北京 100084;3. 中国食品药品检定研究院实验动物资源研究所, 北京 100050, fund=null, authors=王然¹, 马俊¹, 詹欣¹, 辛敏¹, 王婕², 王晓文², 唐劲天², 岳秉飞^1,3, authorsList=王然;马俊;詹欣;辛敏;王婕;王晓文;唐劲天;岳秉飞;)}, authors=[Author(id=1277299994596085881, tenantId=1146029695717560320, journalId=null, articleId=1250863269171376599, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, 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