Article(id=1242140406226432552, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242140382134350274, articleNumber=null, orderNo=14, doi=10.3981/j.issn.1000-7857.2023.07.012, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1655654400000, receivedDateStr=2022-06-20, revisedDate=1669305600000, revisedDateStr=2022-11-25, acceptedDate=null, acceptedDateStr=null, onlineDate=1682587642177, onlineDateStr=2023-04-27, pubDate=1681315200000, pubDateStr=2023-04-13, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1682587642177, onlineIssueDateStr=2023-04-27, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774078950692, creator=sys-migrate, updateTime=1774078950692, updator=sys-migrate, issue=Issue{id=1242140382134350274, tenantId=1146029695717560320, journalId=1146031591421210625, year='2023', volume='41', issue='7', pageStart='1', pageEnd='127', issueExtLink='null', onlineDate='null', pubDate='1681315200000', pubDateStr='2023-04-13', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774078944950, creator='sys-migrate', updateTime=1774078944950, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=114, endPage=126, ext={EN=ArticleExt(id=1242140413411275323, articleId=1242140406226432552, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Isolation and identification of indoor and outdoor culturable microorganisms in Wenchang Spacecraft Launch Site, columnId=1150494644690366681, journalTitle=Science & Technology Review, columnName=Papers, runingTitle=null, highlight=null, articleAbstract=Microorganisms in space station may threaten the health of astronauts and reliability of equipment and affect longterm on-orbit operation of the space station. AIT(assembly, integration, testing) center, transportation and launch environment of spacecraft are the main microbial pollution sources of China's space station. Therefore, it is necessary to conduct comprehensive microbial monitoring of the plant and outdoor environment of Wenchang launch site. Samplings were collected by Petri plate gravitational settling from spacecraft assembly and test clean-room and other five surrounding areas. The strains were identified using traditional culture-based and Sanger dideoxy sequencing technology. Analysis of isolated strains showed that Bacillus sp. and Pseudomonas sp. were the dominant airborne bacterial genus whereas Curvularia sp., Aspergillus sp., and Penicillium sp. dominated the fungal population. Some of the dominant microbes have potential microbial corrosion hazards to the equipment and structures of the space station. The results can provide bacteria and fungi references for space microbial corrosion research in China and facilitate targeted biological safety prevention., authors=LIU Qianqian¹, YAO Qiong², WANG Ning², ZHAO Yong², BAI Miaomiao¹, FENG Yali¹, CHEN Nana¹, XIAO Kui^1*, authorsList=LIU Qianqian, YAO Qiong, WANG Ning, ZHAO Yong, BAI Miaomiao, FENG Yali, CHEN Nana, XIAO Kui, authorCompany=1. Institute of Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China
2. Xichang Satellite Launch Center, Xichang 615000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=GCgT3Y+g2HQmy3W9JfesVQ==, pdfFileSize=2962378, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目（51971032，52071019）), CN=ArticleExt(id=1242140410315874397, articleId=1242140406226432552, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=文昌航天发射场室内外可培养微生物的分离与鉴定, columnId=1242117055294537731, journalTitle=科技导报, columnName=论文, runingTitle=null, highlight=null, articleAbstract=空间站中的微生物可能会威胁到宇航员的健康和设备的可靠性，影响空间站的长期在轨运行。航天器的AIT厂房（总装、集成、测试）、运输和发射环境是中国空间站主要的地面微生物污染源，采用自然沉降法收集了文昌发射场AIT厂房、加注厂房、员工宿舍、发射塔半封闭厂坪、发射塔通风井、环境试验站 6个区域的空气微生物样本，并运用传统的培养法和Sanger测序法对菌株进行了分离鉴定。通过对生物样品的分离纯化，共获得了35株细菌和57株真菌。菌株的基因序列分析表明优势细菌属有芽孢杆菌属（Bacillus）和假单胞菌属（Pseudomonas），优势真菌属有弯孢属（Curvularia）、曲霉属（Aspergillus）和青霉属（Penicillium）。部分优势菌对空间站内部的系统和设备有潜在的微生物腐蚀危害。, authors=刘倩倩¹，姚琼²，王宁²，赵永²，白苗苗¹，冯亚丽¹，陈娜娜¹，肖葵^1*, authorsList=刘倩倩，姚琼，王宁，赵永，白苗苗，冯亚丽，陈娜娜，肖葵, authorCompany=1. 北京科技大学新材料技术研究院腐蚀与防护中心，北京 100083
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