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科技导报
|专题论文
2018
, 36
(16) :
39
-45
微流控芯片在单细胞捕获中的应用
全屏
吴春卉1,2 , 姜有为1,2 , 程鑫1
作者信息
1. 南方科技大学材料科学与工程系, 深圳 518055;
通讯作者:
程鑫(通信作者),教授,研究方向为半导体加工、微流控芯片,电子信箱:chengx@sustc.edu.cn
作者简介:
吴春卉,副研究员,研究方向为微流控芯片,单细胞操控与分析,电子信箱:wuch@sustc.edu.cn
Microfluidic chips for single-cell trapping
WU Chunhui1,2 , JIANG Youwei1,2 , CHENG Xin1
Affiliations
1. Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China;
出版时间: 2018-08-28
doi: 10.3981/j.issn.1000-7857.2018.16.004
文章导航
单细胞捕获是单细胞水平研究的前提和重要组成部分。微流控芯片通常具有与细胞尺寸相当的微通道结构，并能操控纳升至皮升级的极小体积流体，非常适用于高通量的单细胞捕获，加上微流控芯片能够将其他多种操作单元集成在一起，为单细胞分析提供了一种效率高、消耗低的研究平台。概述并对比了多种涉及流体力学、光、电、磁、声等领域的微流控单细胞捕获技术的原理和应用，展望了其未来的研究方向。
微流控芯片
/
单细胞捕获
/
液滴微流控
/
介电电泳
/
光镊
In recent years, the application of the microfluidics in the single cell analysis has attracted more and more attention. The singlecell trapping is the basic and key component for the analysis at the single-cell level. The microfluidic chips usually have single-cell matched microstructures and could achieve the control of extremely small liquid volumes at the nanoliter and picoliter scale. Thus, the microfluidic chips are particularly suitable for the specific or high-throughput single-cell trapping. Moreover, the components for the posttrapping analysis of single cells could be integrated on the chip to build a high-efficiency and low-cost microfluidic single-cell analysis platform. This paper reviews and compares several microfluidic single-cell trapping methods, including hydrodynamic, optical, electrical, magnetic, and acoustic techniques. Future research in the single-cell trapping and analysis on the microfluidic chips is also discussed.
microfluidic chip
/
single-cell trapping
/
droplet microfluidics
/
dielectrophoresis
/
optical tweezer
吴春卉, 姜有为, 程鑫.
微流控芯片在单细胞捕获中的应用.
科技导报,
2018
, 36
(16)
: 39
-45
.
DOI: 10.3981/j.issn.1000-7857.2018.16.004
WU Chunhui, JIANG Youwei, CHENG Xin.
Microfluidic chips for single-cell trapping[J].
Science & Technology Review ,
2018
, 36
(16)
: 39
-45
.
DOI: 10.3981/j.issn.1000-7857.2018.16.004
2018年第36卷第16期
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文章信息
doi: 10.3981/j.issn.1000-7857.2018.16.004
接收时间：2018-05-31
首发时间：2018-08-29
出版时间：2018-08-28
收稿日期：2018-05-31
修回日期：2018-07-29
通讯作者:
程鑫(通信作者),教授,研究方向为半导体加工、微流控芯片,电子信箱:chengx@sustc.edu.cn
https://castjournals.cast.org.cn/joweb/kjdb/CN/10.3981/j.issn.1000-7857.2018.16.004
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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