Article(id=1242135591698113116, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242135568784630300, articleNumber=null, orderNo=16, doi=10.3981/j.issn.1000-7857.2016.24.011, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1448294400000, receivedDateStr=2015-11-24, revisedDate=1479744000000, revisedDateStr=2016-11-22, acceptedDate=null, acceptedDateStr=null, onlineDate=1486437841177, onlineDateStr=2017-02-07, pubDate=1482854400000, pubDateStr=2016-12-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1486437841177, onlineIssueDateStr=2017-02-07, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774077802820, creator=sys-migrate, updateTime=1774077802820, updator=sys-migrate, issue=Issue{id=1242135568784630300, tenantId=1146029695717560320, journalId=1146031591421210625, year='2016', volume='34', issue='24', pageStart='1', pageEnd='143', issueExtLink='null', onlineDate='null', pubDate='1482854400000', pubDateStr='2016-12-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774077797358, creator='sys-migrate', updateTime=1774077797358, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=80, endPage=86, ext={EN=ArticleExt(id=1242135604473963172, articleId=1242135591698113116, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Apoptosis-inducing effects of rare earth substituted phosphotungstic acid containing 5-fluorouracil on HeLa cells, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=As a kind of inorganic metal-oxide cluster compound, polyoxometalates' potential antitumor activities have gained much attention. In this paper, the bioactivities of a series of rare earth substituted phosphotungstic acids containing 5-fluorouracil, K9 (C4 H4 FN2 O2 )2 La(PW11 O39 )2 ·18H2 O, K9 (C4 H4 FN2 O2 )2 Ce(PW11 O39 )2 ·23H2 O, K9 (C4 H4 FN2 O2 )2 Nd(PW11 O39 )2 ·25H2 O, K9 (C4 H4 FN2 O2 )2 Sm(PW11 O39 )2 ·11H2 O and K9 H(C4 H4 FN2 O2 )Eu(PW11 O39 )2 ·11H2 O (abbr. FLnPW, Ln=La, Ce, Nd, Sm, Eu) on HeLa cell are investigated. 5-fluorouracil (abbr. 5-FU) is used as positive control, and phosphotungstate containing 5-fluroracil K11C4 H4 FN2 O2 (PW11 O39 )·7H2 O (abbr. FPW) and phosphotungstic acid H3 PW12 O40 (abbr. PW) are also tested. Morphological analysis shows that typical characteristics of apoptosis appear after treatment with the above compounds (besides PW), and nuclear chromatin is highly concentrated and marginalized. Flow cytometry indicates that S phase cell cycle arrest is induced by compounds (besides PW), whose FPW shows higher S-phase arrest activity than 5-FU, and that the FCePW, FNdPW and FEuPW groups show S phase and G2 /M phase arrests simultaneously. Flow cytometry also shows that all compounds except PW induce apoptosis in HeLa cells, and that the apoptosis-inducing activity order is FLnPW > FPW > 5-FU. Caspase 3 detection shows that caspase 3 activity of HeLa cell is enhanced after treatment with compounds, and the caspase 3 activity order is FLnPW>FPW>5-FU, where the FCePW and FEuPW groups show significant higher activities. These results show that the above compounds containing 5-FU group possess cell cycle arrest activity, apoptosis-inducing activity and caspase 3 inducing activity, while PW could only cause cell death because of acidity. It is suggested that 5-FU group and rare earth elements play most important roles in the antitumor activity, and FLnPW could inhibit cell proliferation by inducing cell cycle arrest, activating the caspase 3-dependent apoptosis pathway., authors=GAN Qiang1 , LIU Xia2 , authorsList=GAN Qiang, LIU Xia, authorCompany=1. State Key Laboratory of Explosion Science and Technology;Beijing Institute of Technology, Beijing 100081, China;9 （C4 H4 FN2 O2 ）2 La（PW11 O39 ）2 ·18H2 O，K9 （C4 H4 FN2 O2 ）2 Ce（PW11 O39 ）2 ·23H2 O，K9 （C4 H4 FN2 O2 ）2 Nd（PW11 O39 ）2 ·25H2 O，K9 （C4 H4 FN2 O2 ）2 Sm（PW11 O39 ）2 ·11H2 O和K9 H（C4 H4 FN2 O2 ）Eu（PW11 O39 ）2 ·11H2 O（FLnPW，Ln=La、Ce、Nd、Sm、Eu）对HeLa细胞凋亡和周期的影响，以5-氟尿嘧啶为阴性对照，同时比较了含5-氟尿嘧啶磷钨酸盐K11C4 H4 FN2 O2 （PW11 O39 ）·7H2 O（FPW）及磷钨酸H3 PW12 O40 （PW）的生物活性。细胞形态检测表明，化合物作用于HeLa细胞后均出现明显凋亡形态特征，细胞核染色质呈高度浓缩和边缘化现象（PW除外）。流式细胞周期检测表明，化合物作用后HeLa细胞均出现S期阻滞，与5-氟尿嘧啶相比，FPW作用后S期阻滞增强，而FCePW、FNdPW和FEuPW组同时出现S期和G2 /M期阻滞。流式细胞检测表明，化合物诱导HeLa细胞发生凋亡（PW除外），且诱导凋亡活性顺序为FLnPW > FPW > 5-氟尿嘧啶。Caspase 3检测表明，化合物作用后Caspase 3活性增强（PW除外），活性顺序与凋亡活性顺序相同，其中FCePW组和FEuPW组Caspase 3相对活性显著增强。实验结果表明，所考查化合物（PW除外）能诱导细胞周期阻滞、诱导凋亡活性以及激活Caspase 3，且FLnPW的活性均高于FPW和5-氟尿嘧啶，而PW只能使肿瘤细胞发生坏死，说明5-氟尿嘧啶和稀土元素对化合物的抗肿瘤活性发挥关键作用，FLnPW可能是通过诱导细胞周期阻滞以及激活Cas-pase 3细胞凋亡通路，实现显著抑制HeLa细胞增殖。, authors=甘强1 , 刘霞2 , authorsList=甘强, 刘霞, authorCompany=1. 北京理工大学爆炸科学与技术国家重点实验室, 北京 100081;
科技导报
| 研究论文 2016, 34(24): 80-86
含5-氟尿嘧啶稀土磷钨酸盐诱导细胞凋亡作用研究
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甘强1 , 刘霞2
作者信息
1. 北京理工大学爆炸科学与技术国家重点实验室, 北京 100081;
通讯作者:
刘霞(通信作者),教授,研究方向为多酸药物化学和光催化作用,电子信箱:liuxia6680@sina.com
Apoptosis-inducing effects of rare earth substituted phosphotungstic acid containing 5-fluorouracil on HeLa cells
Affiliations
出版时间: 2016-12-28
doi: 10.3981/j.issn.1000-7857.2016.24.011
文章导航
多金属氧酸盐作为一种无机金属-氧簇化合物，在抗肿瘤、抗病毒等药物化学领域引起广泛关注。研究了以下5种含5-氟尿嘧啶稀土磷钨酸盐K9 （C4 H4 FN2 O2 ）2 La（PW11 O39 ）2 ·18H2 O，K9 （C4 H4 FN2 O2 ）2 Ce（PW11 O39 ）2 ·23H2 O，K9 （C4 H4 FN2 O2 ）2 Nd（PW11 O39 ）2 ·25H2 O，K9 （C4 H4 FN2 O2 ）2 Sm（PW11 O39 ）2 ·11H2 O和K9 H（C4 H4 FN2 O2 ）Eu（PW11 O39 ）2 ·11H2 O（FLnPW，Ln=La、Ce、Nd、Sm、Eu）对HeLa细胞凋亡和周期的影响，以5-氟尿嘧啶为阴性对照，同时比较了含5-氟尿嘧啶磷钨酸盐K11C4 H4 FN2 O2 （PW11 O39 ）·7H2 O（FPW）及磷钨酸H3 PW12 O40 （PW）的生物活性。细胞形态检测表明，化合物作用于HeLa细胞后均出现明显凋亡形态特征，细胞核染色质呈高度浓缩和边缘化现象（PW除外）。流式细胞周期检测表明，化合物作用后HeLa细胞均出现S期阻滞，与5-氟尿嘧啶相比，FPW作用后S期阻滞增强，而FCePW、FNdPW和FEuPW组同时出现S期和G2 /M期阻滞。流式细胞检测表明，化合物诱导HeLa细胞发生凋亡（PW除外），且诱导凋亡活性顺序为FLnPW > FPW > 5-氟尿嘧啶。Caspase 3检测表明，化合物作用后Caspase 3活性增强（PW除外），活性顺序与凋亡活性顺序相同，其中FCePW组和FEuPW组Caspase 3相对活性显著增强。实验结果表明，所考查化合物（PW除外）能诱导细胞周期阻滞、诱导凋亡活性以及激活Caspase 3，且FLnPW的活性均高于FPW和5-氟尿嘧啶，而PW只能使肿瘤细胞发生坏死，说明5-氟尿嘧啶和稀土元素对化合物的抗肿瘤活性发挥关键作用，FLnPW可能是通过诱导细胞周期阻滞以及激活Cas-pase 3细胞凋亡通路，实现显著抑制HeLa细胞增殖。
多金属氧酸盐
/
5-氟尿嘧啶
/
抗肿瘤
/
凋亡
/
细胞周期阻滞
As a kind of inorganic metal-oxide cluster compound, polyoxometalates' potential antitumor activities have gained much attention. In this paper, the bioactivities of a series of rare earth substituted phosphotungstic acids containing 5-fluorouracil, K9 (C4 H4 FN2 O2 )2 La(PW11 O39 )2 ·18H2 O, K9 (C4 H4 FN2 O2 )2 Ce(PW11 O39 )2 ·23H2 O, K9 (C4 H4 FN2 O2 )2 Nd(PW11 O39 )2 ·25H2 O, K9 (C4 H4 FN2 O2 )2 Sm(PW11 O39 )2 ·11H2 O and K9 H(C4 H4 FN2 O2 )Eu(PW11 O39 )2 ·11H2 O (abbr. FLnPW, Ln=La, Ce, Nd, Sm, Eu) on HeLa cell are investigated. 5-fluorouracil (abbr. 5-FU) is used as positive control, and phosphotungstate containing 5-fluroracil K11C4 H4 FN2 O2 (PW11 O39 )·7H2 O (abbr. FPW) and phosphotungstic acid H3 PW12 O40 (abbr. PW) are also tested. Morphological analysis shows that typical characteristics of apoptosis appear after treatment with the above compounds (besides PW), and nuclear chromatin is highly concentrated and marginalized. Flow cytometry indicates that S phase cell cycle arrest is induced by compounds (besides PW), whose FPW shows higher S-phase arrest activity than 5-FU, and that the FCePW, FNdPW and FEuPW groups show S phase and G2 /M phase arrests simultaneously. Flow cytometry also shows that all compounds except PW induce apoptosis in HeLa cells, and that the apoptosis-inducing activity order is FLnPW > FPW > 5-FU. Caspase 3 detection shows that caspase 3 activity of HeLa cell is enhanced after treatment with compounds, and the caspase 3 activity order is FLnPW>FPW>5-FU, where the FCePW and FEuPW groups show significant higher activities. These results show that the above compounds containing 5-FU group possess cell cycle arrest activity, apoptosis-inducing activity and caspase 3 inducing activity, while PW could only cause cell death because of acidity. It is suggested that 5-FU group and rare earth elements play most important roles in the antitumor activity, and FLnPW could inhibit cell proliferation by inducing cell cycle arrest, activating the caspase 3-dependent apoptosis pathway.
polyoxometalates
/
5-fluororacil
/
antitumor
/
apoptosis
/
cell cycle arrest
甘强, 刘霞.
含5-氟尿嘧啶稀土磷钨酸盐诱导细胞凋亡作用研究.
科技导报,
2016
, 34
(24)
: 80
-86
.
DOI: 10.3981/j.issn.1000-7857.2016.24.011
GAN Qiang, LIU Xia.
Apoptosis-inducing effects of rare earth substituted phosphotungstic acid containing 5-fluorouracil on HeLa cells[J].
Science & Technology Review ,
2016
, 34
(24)
: 80
-86
.
DOI: 10.3981/j.issn.1000-7857.2016.24.011
2016年第34卷第24期
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文章信息
doi: 10.3981/j.issn.1000-7857.2016.24.011
接收时间：2015-11-24
首发时间：2017-02-07
出版时间：2016-12-28
收稿日期：2015-11-24
修回日期：2016-11-22
通讯作者:
刘霞(通信作者),教授,研究方向为多酸药物化学和光催化作用,电子信箱:liuxia6680@sina.com
https://castjournals.cast.org.cn/joweb/kjdb/CN/10.3981/j.issn.1000-7857.2016.24.011
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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