Article(id=1242134571286864268, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242134531768131873, articleNumber=null, orderNo=25, doi=10.3981/j.issn.1000-7857.2016.03.007, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1440000000000, receivedDateStr=2015-08-20, revisedDate=1447689600000, revisedDateStr=2015-11-17, acceptedDate=null, acceptedDateStr=null, onlineDate=1456466424597, onlineDateStr=2016-02-26, pubDate=1455292800000, pubDateStr=2016-02-13, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1456466424597, onlineIssueDateStr=2016-02-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774077559536, creator=sys-migrate, updateTime=1774077559536, updator=sys-migrate, issue=Issue{id=1242134531768131873, tenantId=1146029695717560320, journalId=1146031591421210625, year='2016', volume='34', issue='3', pageStart='1', pageEnd='96', issueExtLink='null', onlineDate='null', pubDate='1455292800000', pubDateStr='2016-02-13', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774077550114, creator='sys-migrate', updateTime=1774077550114, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=85, endPage=90, ext={EN=ArticleExt(id=1242134574625530264, articleId=1242134571286864268, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Regulation of PPARγ and CIDEC expressions in Ad36 induced adipocyte differentiation, columnId=1150494644690366681, journalTitle=Science & Technology Review, columnName=Papers, runingTitle=null, highlight=null, articleAbstract=To investigate the regulation role of Ad36 on PPARγ and CIDEC in Ad36 induced adipocyte differentiation,human adiposederived mesenchymal stem cells(hAMSC)are infected with adenovirus and the adipocyte induced by Ad36 are identified using RT-qPCR and oil red experiments. The expressions of PPARγ and CIDEC genes are determined by RT-qPCR and Western Blotting in Ad36 induced hAMSC. Using GW9662, the PPARγ inhibitor inhibits the expression of PPARγ, then the CIDEC gene expression is detected by Western Blotting in the Ad36 induced hAMSC. Ad36 induced hAMSC differentiates into human adipocyte in vitro, the glucose content in media is reduced and TG content in cells is increased significantly during the differentiation compared to the control group (P<0.05), and the expressions of PPARγ and CIDEC genes are upregulated gradually compared to the control group (P<0.05). CIDEC protein expression is downregulated after treating Ad36 induced human adipocyte by GW9662, the PPARγ specific inhibitor, compared to Ad36 induced group (P<0.05). In this study it is found that Ad36 upregulates CIDEC gene expression through PPARγ in Ad36 induced human adipocytes., authors=AISA Yiliyasi, LIANG Xiaodi, JIAO Yi, GONG Xian, NUERMAIMAITI Nuerbiye, LU Jianfei, GUAN Yaqun, authorsList=AISA Yiliyasi, LIANG Xiaodi, JIAO Yi, GONG Xian, NUERMAIMAITI Nuerbiye, LU Jianfei, GUAN Yaqun, authorCompany=Department of Biochemistry and Molecular Biology, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830011, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=fLEhHtPd0tvOsYGijWNnxg==, pdfFileSize=2163898, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242134573581148562, articleId=1242134571286864268, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=腺病毒36型对PPARγ和CIDEC在脂肪细胞分化过程的调节作用, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为探讨腺病毒36 型在人脂肪细胞分化过程中对PPARγ及CIDEC 基因表达的调节作用,利用腺病毒感染人脂肪源性间充质干细胞(hAMSC)、油红O 染色和RT-qPCR 鉴定Ad36 诱导hAMSC 分化为脂肪细胞模型;葡萄糖氧化酶法和甘油三酯终点法测定人类腺病毒36 型(Ad36)诱导hAMSC 分化为脂肪细胞的过程中培养基葡萄糖浓度及细胞甘油三酯含量;RT-qPCR、Western Blotting 方法检测Ad36 诱导的人脂肪细胞中,PPARγ和CIDEC 蛋白表达水平的变化;用PPARγ特异性抑制剂GW9662 抑制PPARγ表达后,Western Blotting 方法检测Ad36 诱导的人脂肪细胞中CIDEC 蛋白质的表达。Ad36 诱导的hAM-SC 定向分化成人脂肪细胞,分化过程中培养基葡萄糖含量较对照组显著降低(P<0.05),细胞内甘油三酯含量较对照组显著升高(P<0.05),PPARγ和CIDEC 基因表达水平较对照组显著升高(P<0.05),在诱导第6 天表达水平最高,在使用GW9662 抑制PPARγ蛋白质表达后,CIDEC 蛋白质表达水平较对照组显著降低(P<0.05)。从细胞水平证实,Ad36 诱导人脂肪细胞分化过程中,Ad36 通过PPARγ上调CIDEC 基因的表达水平。, authors=伊力亚斯·艾萨, 梁小弟, 焦谊, 弓弦, 努尔比耶·努尔麦麦提, 陆剑飞, 关亚群, authorsList=伊力亚斯·艾萨, 梁小弟, 焦谊, 弓弦, 努尔比耶·努尔麦麦提, 陆剑飞, 关亚群, authorCompany=新疆医科大学基础医学院生物化学与分子生物学教研室, 乌鲁木齐 830011, correspAuthors=null, authorNote=伊力亚斯·艾萨,硕士研究生,研究方向为肥胖和脂代谢,电子信箱:1027992370@qq.com, correspAuthorsNote=关亚群(通信作者),教授,研究方向为肥胖与代谢综合征,电子信箱:yaqunguan@163.com, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=EG2uFf6ostr32zyGrdQbIA==, pdfFileSize=2163898, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目(30960363))}, authors=null, keywords=[Keyword(id=1242134573216244110, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242134571286864268, language=CN, orderNo=1, keyword=人脂肪源性间充质干细胞), Keyword(id=1242134573300130191, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242134571286864268, language=CN, orderNo=1, keyword=腺病毒36), Keyword(id=1242134573379821968, tenantId=1146029695717560320, 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