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articleAbstract=从长期受对硝基酚(para-nitrophenol,PNP)污染的土壤中,分离到一株能够降解对硝基酚并以其作为唯一碳源和氮源生长的菌株,命名为PN-1。经16S rDNA 序列分析,初步鉴定该菌株为假单胞菌(Pseudomona)。通过研究其对PNP 的降解、重金属以及抗生素抗性、植物促生等特性,得到如下结果:该菌株对PNP 的最大耐受质量浓度为200 mg/L,具有卡那霉素和氯霉素抗性,对4 种重金属Cd2+、Pb2+、Zn2+和Cr6+的最低抑制浓度(minimum inhibitory concentration,MIC)分别为700、500、800 和100 mg/L,同时具有产吲哚乙酸(IAA)和铁载体、1-氨基环丙烷-1-羧酸(ACC)脱氨酶活性以及溶磷能力。通过用荧光蛋白标记后能在土壤中正常存活,具有能与植物联合修复PNP 污染土壤的潜力,有较好的使用前景。, authors=任何军, 王聪, authorsList=任何军, 王聪, authorCompany=吉林大学环境与资源学院, 长春 130061, correspAuthors=null, authorNote=任何军,副教授,研究方向为微生物技术,电子信箱:renhejun@126.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=r5BTR3Qy/KuD8byvxs+9Cg==, pdfFileSize=3170118, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, 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一株对硝基酚降解菌的筛选及其植物促生特性
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一株对硝基酚降解菌的筛选及其植物促生特性
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任何军, 王聪
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    吉林大学环境与资源学院, 长春 130061
Isolation of a para-nitrophenol degrading bacterium and characterization of its plant growth-promoting properties
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出版时间: 2016-01-28 doi: 10.3981/j.issn.1000-7857.2016.2.039
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从长期受对硝基酚(para-nitrophenol,PNP)污染的土壤中,分离到一株能够降解对硝基酚并以其作为唯一碳源和氮源生长的菌株,命名为PN-1。经16S rDNA 序列分析,初步鉴定该菌株为假单胞菌(Pseudomona)。通过研究其对PNP 的降解、重金属以及抗生素抗性、植物促生等特性,得到如下结果:该菌株对PNP 的最大耐受质量浓度为200 mg/L,具有卡那霉素和氯霉素抗性,对4 种重金属Cd2+、Pb2+、Zn2+和Cr6+的最低抑制浓度(minimum inhibitory concentration,MIC)分别为700、500、800 和100 mg/L,同时具有产吲哚乙酸(IAA)和铁载体、1-氨基环丙烷-1-羧酸(ACC)脱氨酶活性以及溶磷能力。通过用荧光蛋白标记后能在土壤中正常存活,具有能与植物联合修复PNP 污染土壤的潜力,有较好的使用前景。
对硝基酚  /  重金属  /  抗生素  /  植物促生特性  /  荧光蛋白标记
A para-nitrophenol (PNP) degrading strain, named PN-1, was isolated from the PNP-contaminated soil. PN-1 utilized PNP as the sole carbon and nitrogen source during the cultivation. Based on the result of 16S rDNA gene sequence, the strain was preliminarily designated as Pseudomona. Experiments were carried out to investigate the degradation of PNP, heavy metal, antibiotic, plant growthpromoting (PGP) properties and GFP-tagging of PN-1. The results showed that the maximum tolerant dose of TM to PNP was 200 mg/L, and the MICs for the following four heavy metals were 700 mg/L for Cd2+, 500 mg/L for Pb2+, 800 mg/L for Zn2+, and 100 mg/L for Cr6+. What is more, PN-1 could grow in media with chloromycetin and ampicillin. PGP traits of PN-1 included IAA, siderophore production, ACC deaminase activity and phosphate solubilizing activity. GFP-tagged PN-1 could survive and bioremediate PNP-contaminated soil with plants.
paranitrophenol  /  heavy metal  /  antibiotic  /  plant growth promotion properties  /  GFP-tagging
任何军, 王聪. 一株对硝基酚降解菌的筛选及其植物促生特性. 科技导报, 2016 , 34 (2) : 231 -236 . DOI: 10.3981/j.issn.1000-7857.2016.2.039
REN Hejun, WANG Cong. Isolation of a para-nitrophenol degrading bacterium and characterization of its plant growth-promoting properties[J]. Science & Technology Review, 2016 , 34 (2) : 231 -236 . DOI: 10.3981/j.issn.1000-7857.2016.2.039
2016年第34卷第2期
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doi: 10.3981/j.issn.1000-7857.2016.2.039
  • 接收时间:2015-06-04
  • 首发时间:2016-02-04
  • 出版时间:2016-01-28
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  • 收稿日期:2015-06-04
  • 修回日期:2015-09-24
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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