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2. State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;
3. Fujian Chaoda Modern Agriculture Technology Research Institute, Fuzhou 350003, China;
4. College of Materials Science and Engineering, Fuzhou University, Fuzhou 350108, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=AR2/U7YN9MEkEZ/GPEEdmQ==, pdfFileSize=2146910, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242133330922123766, articleId=1242133326941729260, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=耐低温降解生物质废弃物复合菌剂的混料设计优化, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为了筛选制备耐低温高效降解农业生物质废弃物的复合菌剂,在已获得的耐低温木质纤维素降解菌群A25-3 的基础上,采用混料设计(mixture design)对复合菌比例进行优化,通过测定菌群A25-3、酿酒酵母(Saccharomyces cerevisiae)和绿色木霉(Trichoderma viride)不同比例搭配对纤维素酶(CMCase、滤纸酶和外切葡聚糖酶)、蛋白酶和α-淀粉酶活性的影响,建立了各菌株(群)搭配比例与试验指标之间的回归方程。使用Design Expert 8.0 软件的优化功能对满足所有期望的响应值进行优化,得到复合菌的最优配比(质量分数)为绿色木霉37.24%,菌群A25-3 67.76%。对该最优比例进行验证试验,得到的结果与预测值基本一致。秸秆降解试验表明,固体复合菌剂可高效降解秸秆,有效提高堆肥体系的温度,在低温环境下的堆肥和秸秆腐熟具有很好的应用前景。, authors=罗立津1,2,3, 万立2, 陈宏1, 徐福乐3, 贾纬1, 聂毅磊1, 温翠莲4, authorsList=罗立津, 万立, 陈宏, 徐福乐, 贾纬, 聂毅磊, 温翠莲, authorCompany=1. 福建省微生物研究所, 福建省新药(微生物)筛选重点实验室, 福州350007;
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3. 福建超大现代农业科技研究所, 福州350003;
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耐低温降解生物质废弃物复合菌剂的混料设计优化
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科技导报 | 研究论文 2015, 33(8): 24-29
耐低温降解生物质废弃物复合菌剂的混料设计优化
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罗立津1,2,3, 万立2, 陈宏1, 徐福乐3, 贾纬1, 聂毅磊1, 温翠莲4
作者信息
    1. 福建省微生物研究所, 福建省新药(微生物)筛选重点实验室, 福州350007;
    2. 中国农业大学农业生物技术国家重点实验室;中国农业大学生物学院, 北京100193;
    3. 福建超大现代农业科技研究所, 福州350003;
    4. 福州大学材料科学与工程学院, 福州350108

通讯作者:

温翠莲,博士,研究方向为环境友好生态材料,电子信箱:clwen@fzu.edu.cn
Optimal mixture design of preparation of cold-adapted multiple species inoculant for biomass waste degradation
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出版时间: 2015-04-28 doi: 10.3981/j.issn.1000-7857.2015.08.003
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为了筛选制备耐低温高效降解农业生物质废弃物的复合菌剂,在已获得的耐低温木质纤维素降解菌群A25-3 的基础上,采用混料设计(mixture design)对复合菌比例进行优化,通过测定菌群A25-3、酿酒酵母(Saccharomyces cerevisiae)和绿色木霉(Trichoderma viride)不同比例搭配对纤维素酶(CMCase、滤纸酶和外切葡聚糖酶)、蛋白酶和α-淀粉酶活性的影响,建立了各菌株(群)搭配比例与试验指标之间的回归方程。使用Design Expert 8.0 软件的优化功能对满足所有期望的响应值进行优化,得到复合菌的最优配比(质量分数)为绿色木霉37.24%,菌群A25-3 67.76%。对该最优比例进行验证试验,得到的结果与预测值基本一致。秸秆降解试验表明,固体复合菌剂可高效降解秸秆,有效提高堆肥体系的温度,在低温环境下的堆肥和秸秆腐熟具有很好的应用前景。
混料设计  /  复合菌剂  /  纤维素酶  /  耐低温  /  生物质废弃物
In this paper, an optimum mixture design is used for the preparation of cold-adapted multiple species inoculant. Based on the Design Expert software, a quadratic model is established as a function of the component fractions of species, such as the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3, on the enzyme activity of the CMCase, the FPase, the cellobiohydrolase, the proteinase and the α-amylase. The response values satisfying all expectations are optimized,and the most excellent combinations of the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3 are 0%, 37.24% and 67.76%, respectively. The result of the verification experiment on the formulation is consistent with the prediction. The straw degradation experiments show that the multiple species inoculant has a high level of degradation efficiency for the straw at a low temperature. It suggests that there is a great application prospect of the multiple species inoculant under cold conditions.
mixture design  /  multiple species inoculant  /  cellulase  /  cold-adapted  /  biomass waste
罗立津, 万立, 陈宏, 徐福乐, 贾纬, 聂毅磊, 温翠莲. 耐低温降解生物质废弃物复合菌剂的混料设计优化. 科技导报, 2015 , 33 (8) : 24 -29 . DOI: 10.3981/j.issn.1000-7857.2015.08.003
LUO Lijin, WAN Li, CHEN Hong, XU Fule, JIA Wei, NIE Yilei, WEN Cuilian. Optimal mixture design of preparation of cold-adapted multiple species inoculant for biomass waste degradation[J]. Science & Technology Review, 2015 , 33 (8) : 24 -29 . DOI: 10.3981/j.issn.1000-7857.2015.08.003
2015年第33卷第8期
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doi: 10.3981/j.issn.1000-7857.2015.08.003
  • 接收时间:2015-02-10
  • 首发时间:2015-05-05
  • 出版时间:2015-04-28
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  • 收稿日期:2015-02-10
  • 修回日期:2015-03-26
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温翠莲,博士,研究方向为环境友好生态材料,电子信箱:clwen@fzu.edu.cn
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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