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桑叶金花茶中酚类物质动态变化及其抗氧化活性
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科技导报 |研究论文 2015 , 33 (7) : 95 -99
桑叶金花茶中酚类物质动态变化及其抗氧化活性
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王吉成1, 刘轩2, 邹先伟2, 李倩2, 唐劲天2, 翟延君1
作者信息
    1. 辽宁中医药大学药学院, 大连 116600;
    2. 清华大学工程物理系, 北京 100084
通讯作者:
翟延君,教授,研究方向为中药品质评价与鉴定,电子信箱:lnzyzyj@sohu.com
作者简介:
王吉成,硕士研究生,研究方向为中药品质评价与鉴定,电子信箱:419723822@qq.com
Dynamic change and antioxidant activity of phenolics in the mulberry leaf and Jinhua tea
  • WANG Jicheng1, LIU Xuan2, ZOU Xianwei2, LI Qian2, TANG Jintian2, ZHAI Yanjun1
  • Affiliations
      1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;
      2. Department of Engineering Physics, Tsinghua University, Beijing 100084, China
    出版时间: 2015-04-13 doi: 10.3981/j.issn.1000-7857.2015.07.016
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    为探讨桑叶固体双向发酵过程中水溶性酚类物质含量的动态变化及其抗氧化活性,应用三氯化铝比色法、Folin-酚法测定桑叶固体双向发酵物中水溶性总黄酮及总酚酸含量,并以清除1-1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)、铁还原能力3 个指标,评价桑叶固体双向发酵过程中体外抗氧化活性的变化情况。结果发现,随着发酵时间的延长,桑叶固体双向发酵物中水溶性总黄酮、总酚酸均呈先增加后减少的趋势。其中发酵14 d 水溶性黄酮质量分数最多为2.16 mg/g,20 d 水溶性总酚酸质量分数最多为1.48 mg/g。对于体外3 个抗氧化指标而言,整体表现为:14 d>7 d>11 d>20 d>40 d 。
    固体双向发酵  /  桑叶金花茶  /  总黄酮  /  总酚酸  /  体外抗氧化
    This paper aims to investigate the dynamic change and antioxidant activity of water soluble phenolic substances in mulberry leaves and Eurotium cristatum solid bi-directional fermentation process. The contents of the water soluble total flavonoids and total phenolics were measured by using AlCl3 method and Folin-Ciocalteu method. At the same time, the antioxidant activity changes of the solid bi-directional fermentation process were evaluated by three in vitro experiments including DPPH assay, ABTS+ assay and ferric-reducing power assay. The results showed that during the fermentation process, the water soluble total flavonoids and total phenolics decreased after the initial increase. The water soluble flavonoid content after 14 days' fermentation was the highest, being 2.16 mg/g, and the water soluble phenolics content after 20 days' fermentation was the highest, being 1.48 mg/g. The antioxidant activity displayed an order of 14 d>7 d>11 d>20 d>40 d.
    solid bi-directional fermentation  /  mulberry leaves and Jinhua tea  /  total flavonoids  /  total phenolics  /  in vitro antioxidant activity
    王吉成, 刘轩, 邹先伟, 李倩, 唐劲天, 翟延君. 桑叶金花茶中酚类物质动态变化及其抗氧化活性. 科技导报, 2015 , 33 (7) : 95 -99 . DOI: 10.3981/j.issn.1000-7857.2015.07.016
    WANG Jicheng, LIU Xuan, ZOU Xianwei, LI Qian, TANG Jintian, ZHAI Yanjun. Dynamic change and antioxidant activity of phenolics in the mulberry leaf and Jinhua tea[J]. Science & Technology Review, 2015 , 33 (7) : 95 -99 . DOI: 10.3981/j.issn.1000-7857.2015.07.016

      中国博士后科学基金特别资助项目(2014T70068)

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    doi: 10.3981/j.issn.1000-7857.2015.07.016
    • 接收时间:2014-12-23
    • 首发时间:2015-05-04
    • 出版时间:2015-04-13
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    • 收稿日期:2014-12-23
    • 修回日期:2015-03-13
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    通讯作者:

    翟延君,教授,研究方向为中药品质评价与鉴定,电子信箱:lnzyzyj@sohu.com
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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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