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科技导报
| 研究论文 2015, 33(7): 84-89
春小麦重组自交系及多种SSR标记构建遗传图谱
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董建力1 , 白海波1 , 朱永兴1 , 赵朋2 , 吕学莲1 , 李树华1 , 王中华2
作者信息
1. 宁夏农林科学院农业生物技术研究中心, 银川 750002;
2. 西北农林科技大学农学院, 杨陵 712100
通讯作者:
王中华,教授,研究方向为小麦抗逆生理及分子生物学,电子信箱:zhonghuawang@nwsuaf.edu.cn
Construction of wheat linkage map using a set of spring wheat recombinant inbred lines and different SSR markers
Affiliations
出版时间: 2015-04-13
doi: 10.3981/j.issn.1000-7857.2015.07.014
文章导航
以春小麦重组自交系(RIL)宁春4 号×宁春27 号为作图群体,利用SSR 标记构建小麦遗传连锁图谱。结果表明,用1001对SSR 引物选出亲本间表现多态性的引物307 对,多态性频率为30.7%。利用307 对多态性引物对RIL 群体进行分析,共检测到266 个多态性标记位点。通过χ 2 检测(P <0.05),有147 个SSR 标记表现为偏分离,偏分离率为55.3%,129 个偏向母本宁春4号,其偏分离位点主要分布在B 和D 基因组上。用Mapmaker 3.0 和Mapdraw 2.1 软件将266 个SSR 位点绘制在小麦遗传连锁图上,该图谱覆盖小麦基因组全长2187.79 cM,标记间的平均遗传距离为8.22 cM。
春小麦
/
重组自交系
/
SSR 标记
/
连锁图谱
The recombinant inbred lines (RILs) between Ningchun 4 and Ningchun 27 are used as the donors for the construction of the spring wheat genetic linkage map by different SSR markers. It is revealed that 307 pairs of the SSR markers in total are available to show the SSR polymorphism differences between the two parents among 1001 pairs of SSR markers originally selected. The polymorphism frequency is 30.7%. The 307 pairs of the SSR primers with the polymorphisms between Ningchun 4 and Ningchun 27 are applied to further analyze the RIL population, and 266 loci with polymorphism are detected by the primers mentioned above. The χ 2 test (P <0.05) shows that 147 SSR markers see a significant segregation distortion of 55.3%, mostly distributed on the B and D genomes. Among them, the segregated distortions of 129 SSR markers are resulted from the female parent of Ningchun 4. By using the software of Mapmaker 3.0 and Mapdraw 2.1, 266 SSR markers are assigned on the linkage genetic map of the wheat, which covers about 2187.79 cM of the wheat genome with an average distance of 8.22 cM between markers.
spring wheat
/
recombinant inbred lines
/
SSR markers
/
linkage map
董建力, 白海波, 朱永兴, 赵朋, 吕学莲, 李树华, 王中华.
春小麦重组自交系及多种SSR标记构建遗传图谱.
科技导报,
2015
, 33
(7)
: 84
-89
.
DOI: 10.3981/j.issn.1000-7857.2015.07.014
DONG Jianli, BAI Haibo, ZHU Yongxing, ZHAO Peng, LÜ Xuelian, LI Shuhua, WANG Zhonghua.
Construction of wheat linkage map using a set of spring wheat recombinant inbred lines and different SSR markers[J].
Science & Technology Review ,
2015
, 33
(7)
: 84
-89
.
DOI: 10.3981/j.issn.1000-7857.2015.07.014
2015年第33卷第7期
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文章信息
doi: 10.3981/j.issn.1000-7857.2015.07.014
接收时间:2014-10-14
首发时间:2015-05-04
出版时间:2015-04-13
收稿日期:2014-10-14
修回日期:2015-01-23
通讯作者:
王中华,教授,研究方向为小麦抗逆生理及分子生物学,电子信箱:zhonghuawang@nwsuaf.edu.cn
https://castjournals.cast.org.cn/joweb/kjdb/CN/10.3981/j.issn.1000-7857.2015.07.014
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2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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