Article(id=1242132925873987660, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242132909226799308, articleNumber=null, orderNo=11, doi=10.3981/j.issn.1000-7857.2014.36.009, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1401033600000, receivedDateStr=2014-05-26, revisedDate=1413302400000, revisedDateStr=2014-10-15, acceptedDate=null, acceptedDateStr=null, onlineDate=1420771758047, onlineDateStr=2015-01-09, pubDate=1419696000000, pubDateStr=2014-12-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1420771758047, onlineIssueDateStr=2015-01-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774077167238, creator=sys-migrate, updateTime=1774077167238, updator=sys-migrate, issue=Issue{id=1242132909226799308, tenantId=1146029695717560320, journalId=1146031591421210625, year='2014', volume='32', issue='36', pageStart='1', pageEnd='128', issueExtLink='null', onlineDate='null', pubDate='1419696000000', pubDateStr='2014-12-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=null, issueType=-1, specialIssue=null, createTime=1774077163269, creator='sys-migrate', updateTime=1774077163269, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=59, endPage=64, ext={EN=ArticleExt(id=1242132929451733246, articleId=1242132925873987660, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Primer Screening and Establishment of AFLP Marked System of Pst Ⅰ and Mse Ⅰ Endonuclease Combination in Sunflower, columnId=null, journalTitle=Science & Technology Review, columnName=null, runingTitle=null, highlight=null, articleAbstract=To provide technical support for construction of genetic linkage map and molecular markers assisted breeding of sunflower, an optimized AFLP reaction system using double-digestion of Pst Ⅰ and Mse Ⅰ endonuclease has been established. The extraction method of genomic DNA, the DNA digestion and ligation system and several key factors affecting the PCR selective amplification are optimized. In addition, the primer combination which suits for analysis of AFLP is also screened in sunflower. The results show that the high quality of genomic DNA as PCR template can be extracted with the improved CTAB method, and that the DNA digestion and ligation can be used as a one-step process with the reaction time of 14 hours. In the optimized selection amplification system of total volume of 20 μL, the Mg²⁺ concentration is 2 mmol/L, dNTPs concentration is 0.3 mmol/L, the primers concentration is 0.6 mmol/L and the products of pre-amplification for selective amplification are diluted to 30 times. Moreover, 48 pairs stable and polymorphisms primer have been selected for AFLP analysis in sunflower., authors=ZHANG Yonghu, HOU Jianhua, LÜ Pin, YU Zhixian, authorsList=ZHANG Yonghu, HOU Jianhua, LÜ Pin, YU Zhixian, authorCompany=Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=M15A3POvC2De/yihByTofQ==, pdfFileSize=1324288, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242132927325221105, articleId=1242132925873987660, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=向日葵Pst Ⅰ和Mse Ⅰ内切酶组合AFLP标记体系的建立及引物筛选, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为建立并优化适合向日葵的Pst Ⅰ 和Mse Ⅰ 组合的AFLP 技术体系, 为下一步构建遗传连锁图谱及开展分子标记辅助育种奠定基础, 对向日葵基因组DNA 提取方法、酶切连接体系和选择性扩增程序的影响因素进行优化, 并对适合向日葵AFLP 分析的引物组合进行筛选。结果表明, 模板DNA 的提取采用改进CTAB 法, 酶切连接采用一步法反应14 h, 在选择性扩增体系20 μL中, Mg²⁺和dNTP 浓度分别为2 mmol/L 和0.3 mmol/L, 选扩引物的浓度为0.6 mmol/L, 预扩增产物最适稀释倍数为30 倍。同时筛选出了稳定且多态性丰富的48 对适合向日葵AFLP 分析的引物组合。, authors=张永虎, 侯建华, 吕品, 于志贤, authorsList=张永虎, 侯建华, 吕品, 于志贤, authorCompany=内蒙古农业大学农学院, 呼和浩特010018, correspAuthors=null, authorNote=张永虎, 博士研究生, 研究方向为作物抗性遗传改良, 电子信箱:zhangyonghu0815@126.com, correspAuthorsNote=侯建华, 教授, 研究方向为作物抗逆性状的遗传改良, 电子信箱:Houjh68@163.com, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=+IU9dcimqP1pKvZg4JNWrQ==, pdfFileSize=1324288, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, 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