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In order to reveal the nucleotide polymorphisms in the maize ELM1 gene and their associations with important agronomic traits, the sequences of the maize ELM1 gene are captured in 80 inbred lines in the present study, and the associations between the sequence variations of this gene and 2 plant types (including plant height and ear height) and 7 ear traits (ear length, ear diameter, cob diameter, ear weight, kernels per row, rows per ear and kernels per ear) are determined. A total of 85 variants, including 73 SNPs and 12 indels, are detected from the full sequences of this gene in the tested inbred lines. Although in the coding regions, the indel is not included, with the SNPs of CDS sequences, the maize ELM1 gene can be classified into 7 haplotypes, which encode 6 different ELM1 proteins. There are significant differences among inbred lines for all 9 plant types and ear traits. According to the results of the association analysis, one nonsynonymous SNP is found to be associated with the ear height, while two other nonsynonymous SNPs are associated with the kernels per row., authors=YANG Zefeng, ZHANG Enying, XU Shuhui, MAO Beili, PAN Liang, CHEN Qing, XU Chenwu, authorsList=YANG Zefeng, ZHANG Enying, XU Shuhui, MAO Beili, PAN Liang, CHEN Qing, XU Chenwu, authorCompany=Key Laboratory of the Ministry of Education for Plant Functional Genomics; Jiangsu Key Laboratory of Crop Genetics and Physiology; Agricultural College, Yangzhou University, Yangzhou 225009, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=zPCJKjTPCrKIDhSSEh0lPw==, pdfFileSize=2807113, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, 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玉米ELM1基因的序列变异及与株型和穗部相关性状的关联分析
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科技导报 | 研究论文 2014, 32(35): 78-84
玉米ELM1基因的序列变异及与株型和穗部相关性状的关联分析
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杨泽峰, 张恩盈, 徐暑晖, 毛蓓丽, 潘亮, 陈庆, 徐辰武
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    扬州大学农学院;教育部植物功能基因组学重点实验室;江苏省作物遗传生理重点实验室, 扬州 225009

通讯作者:

徐辰武,教授,研究方向为数量遗传学与生物信息学,电子信箱:qtls@yzu.edu.cn
Sequence Variations of the Maize ELM1 Gene and Their Association with Plant Types and Ear Traits
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出版时间: 2014-12-18 doi: 10.3981/j.issn.1000-7857.2014.35.009
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玉米elm1 突变体使得光敏色素载色体合成受阻并导致光敏色素活性下降,从而使得突变体植株表现出对光的不敏感性.为研究玉米ELM1 基因序列的多态性及其与主要农艺性状之间的关联,本研究对玉米ELM1 基因在80 个自交系中进行了目标序列重测序,并与株高和穗位高2 个株型性状以及穗长、穗粗、轴粗、穗重、行粒数、穗行数和穗粒数7 个穗部性状进行关联分析.ELM1 基因在供试玉米自交系中共有85 个变异,包括73 个SNP 和12 个Indel.尽管该基因的编码区不含Indel,但15 个SNP 变异位点依然可以将编码区划分成7 种单倍型,并编码6 种ELM1 蛋白质.关联分析发现,玉米ELM1 基因中1 个非同义突变位点与穗位高存在显著关联,另有2 个非同义突变位点与行粒数存在显著关联.
玉米  /  ELM1 基因  /  序列变异  /  株型和穗部性状  /  关联分析
With the deficiency of the synthesis for the phytochromechromophore in the maize elm1 mutant, the phytochrome activity might be reduced and the mutant plant might show characteristics of light insensitivity. In order to reveal the nucleotide polymorphisms in the maize ELM1 gene and their associations with important agronomic traits, the sequences of the maize ELM1 gene are captured in 80 inbred lines in the present study, and the associations between the sequence variations of this gene and 2 plant types (including plant height and ear height) and 7 ear traits (ear length, ear diameter, cob diameter, ear weight, kernels per row, rows per ear and kernels per ear) are determined. A total of 85 variants, including 73 SNPs and 12 indels, are detected from the full sequences of this gene in the tested inbred lines. Although in the coding regions, the indel is not included, with the SNPs of CDS sequences, the maize ELM1 gene can be classified into 7 haplotypes, which encode 6 different ELM1 proteins. There are significant differences among inbred lines for all 9 plant types and ear traits. According to the results of the association analysis, one nonsynonymous SNP is found to be associated with the ear height, while two other nonsynonymous SNPs are associated with the kernels per row.
maize  /  ELM1 gene  /  sequence variations  /  plant type and ear traits  /  association analysis
杨泽峰, 张恩盈, 徐暑晖, 毛蓓丽, 潘亮, 陈庆, 徐辰武. 玉米ELM1基因的序列变异及与株型和穗部相关性状的关联分析. 科技导报, 2014 , 32 (35) : 78 -84 . DOI: 10.3981/j.issn.1000-7857.2014.35.009
YANG Zefeng, ZHANG Enying, XU Shuhui, MAO Beili, PAN Liang, CHEN Qing, XU Chenwu. Sequence Variations of the Maize ELM1 Gene and Their Association with Plant Types and Ear Traits[J]. Science & Technology Review, 2014 , 32 (35) : 78 -84 . DOI: 10.3981/j.issn.1000-7857.2014.35.009
2014年第32卷第35期
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doi: 10.3981/j.issn.1000-7857.2014.35.009
  • 接收时间:2014-10-15
  • 首发时间:2014-12-25
  • 出版时间:2014-12-18
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  • 收稿日期:2014-10-15
  • 修回日期:2014-11-03
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徐辰武,教授,研究方向为数量遗传学与生物信息学,电子信箱:qtls@yzu.edu.cn
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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