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It was found to be involved in the abiotic stress responsesof crops. A genome-wide survey of BAM genes in 5 grass species was performed, including rice, maize, sorghum, Setaria, and Brachypodium, by describing their phylogenetic relationships, functional divergence, and adaptive evolution. The phylogeny classified the gramineous BAM genes into 10 clusters of orthologous genes (COGs), and the genes in the same COG shared the syntenic region. Functional divergence analysis provided statistical evidence that both the shift in the evolutionary rate pattern and cluster-specific alterations of amino acid physiochemical properties contributed to COG-specific functional evolution of BAM genes in grasses. In addition, 3 COGs were found to be influenced by positive selection through maximum likelihood analysis. The expression patterns of rice BAM genes were investigated, and the resultsrevealed that they were differentially expressed under the treatments of abiotic stresses. These observations may provide useful references for further functional detection of BAM genes in grasses., authors=YANG Zefeng, XU Shuhui, WANG Yifan, ZHANG Enying, XU Chenwu, authorsList=YANG Zefeng, XU Shuhui, WANG Yifan, ZHANG Enying, XU Chenwu, authorCompany=Jiangsu Key Laboratory of Crop Genetics and Physiology; Key Laboratory of the Ministry of Education for Plant Functional Genomics; Agricultural College, Yangzhou University, Yangzhou 225009, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=6RqfOJjuEAVEeQu3AYVxzA==, pdfFileSize=5846792, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242132704205026071, articleId=1242132701684249357, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=禾本科植物β-淀粉酶基因家族分子进化及响应非生物胁迫的表达模式分析, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=β-淀粉酶(beta-amylase,BAM)是一类关键的淀粉水解酶,在禾谷类作物生长发育过程中起着重要作用,与植物多种非生物胁迫响应相关.本研究通过系统发育分析,将水稻、玉米、高粱、谷子、二穗短柄草5 种禾本科植物中共54 个BAM 基因分为10 个同源基因簇,每个同源基因簇都涵盖了这5 个物种,因此推测在禾本科祖先物种中至少含有10 个BAM 基因,并且在禾本科植物分化后没有发生明显的基因丢失事件.基于对编码蛋白质序列的功能分化分析,表明同源基因簇间存在明显的进化速率的差异.对10 个同源基因簇进行了适应性进化检测,发现有3 个同源簇在禾本科植物的进化过程中经历了适应性进化.此外,对水稻β-淀粉酶的表达分析发现,一些β-淀粉酶具有组织特异性表达特征,并且至少有5 个水稻的β-淀粉酶基因具有受到非生物逆境的胁迫而表现出不同的表达模式.本研究结果为进一步探讨禾本科BAM 基因的生物学功能提供了一定的理论基础., authors=杨泽峰, 徐暑晖, 王一凡, 张恩盈, 徐辰武, authorsList=杨泽峰, 徐暑晖, 王一凡, 张恩盈, 徐辰武, authorCompany=扬州大学农学院;教育部植物功能基因组学重点实验室;江苏省作物遗传生理重点实验室, 扬州 225009, correspAuthors=null, authorNote=杨泽峰,副教授,研究方向为植物功能基因组学与生物信息学,电子信箱:zfyang@yzu.edu.cn, correspAuthorsNote=徐辰武,教授,研究方向为数量遗传学与生物信息学,电子信箱:qtls@yzu.edu.cn, copyrightStatement=null, 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禾本科植物β-淀粉酶基因家族分子进化及响应非生物胁迫的表达模式分析
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杨泽峰, 徐暑晖, 王一凡, 张恩盈, 徐辰武
科技导报 | 研究论文 2014,32(31): 29-36
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科技导报 |研究论文 2014 , 32 (31) : 29 -36
禾本科植物β-淀粉酶基因家族分子进化及响应非生物胁迫的表达模式分析
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杨泽峰, 徐暑晖, 王一凡, 张恩盈, 徐辰武
作者信息
通讯作者:
徐辰武,教授,研究方向为数量遗传学与生物信息学,电子信箱:qtls@yzu.edu.cn
作者简介:
杨泽峰,副教授,研究方向为植物功能基因组学与生物信息学,电子信箱:zfyang@yzu.edu.cn
Molecluar Evolution and Expression Patterns Under Abiotic Stresses of Beta-amylase Gene Family in Grasses
YANG Zefeng, XU Shuhui, WANG Yifan, ZHANG Enying, XU Chenwu
Affiliations
    Jiangsu Key Laboratory of Crop Genetics and Physiology; Key Laboratory of the Ministry of Education for Plant Functional Genomics; Agricultural College, Yangzhou University, Yangzhou 225009, China
出版时间: 2014-11-08 doi: 10.3981/j.issn.1000-7857.2014.31.002
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β-淀粉酶(beta-amylase,BAM)是一类关键的淀粉水解酶,在禾谷类作物生长发育过程中起着重要作用,与植物多种非生物胁迫响应相关.本研究通过系统发育分析,将水稻、玉米、高粱、谷子、二穗短柄草5 种禾本科植物中共54 个BAM 基因分为10 个同源基因簇,每个同源基因簇都涵盖了这5 个物种,因此推测在禾本科祖先物种中至少含有10 个BAM 基因,并且在禾本科植物分化后没有发生明显的基因丢失事件.基于对编码蛋白质序列的功能分化分析,表明同源基因簇间存在明显的进化速率的差异.对10 个同源基因簇进行了适应性进化检测,发现有3 个同源簇在禾本科植物的进化过程中经历了适应性进化.此外,对水稻β-淀粉酶的表达分析发现,一些β-淀粉酶具有组织特异性表达特征,并且至少有5 个水稻的β-淀粉酶基因具有受到非生物逆境的胁迫而表现出不同的表达模式.本研究结果为进一步探讨禾本科BAM 基因的生物学功能提供了一定的理论基础.
β-淀粉酶  /  同源基因簇  /  功能分化  /  正选择作用
Beta-amylase (BAM) plays a central role in the complete degradation of starch tometabolisable or fermentable sugars during the germination or maltingof cereal grains. It was found to be involved in the abiotic stress responsesof crops. A genome-wide survey of BAM genes in 5 grass species was performed, including rice, maize, sorghum, Setaria, and Brachypodium, by describing their phylogenetic relationships, functional divergence, and adaptive evolution. The phylogeny classified the gramineous BAM genes into 10 clusters of orthologous genes (COGs), and the genes in the same COG shared the syntenic region. Functional divergence analysis provided statistical evidence that both the shift in the evolutionary rate pattern and cluster-specific alterations of amino acid physiochemical properties contributed to COG-specific functional evolution of BAM genes in grasses. In addition, 3 COGs were found to be influenced by positive selection through maximum likelihood analysis. The expression patterns of rice BAM genes were investigated, and the resultsrevealed that they were differentially expressed under the treatments of abiotic stresses. These observations may provide useful references for further functional detection of BAM genes in grasses.
beta-amylase  /  cluster of orthologous genes  /  functional divergence  /  positive selection
杨泽峰, 徐暑晖, 王一凡, 张恩盈, 徐辰武. 禾本科植物β-淀粉酶基因家族分子进化及响应非生物胁迫的表达模式分析. 科技导报, 2014 , 32 (31) : 29 -36 . DOI: 10.3981/j.issn.1000-7857.2014.31.002
YANG Zefeng, XU Shuhui, WANG Yifan, ZHANG Enying, XU Chenwu. Molecluar Evolution and Expression Patterns Under Abiotic Stresses of Beta-amylase Gene Family in Grasses[J]. Science & Technology Review, 2014 , 32 (31) : 29 -36 . DOI: 10.3981/j.issn.1000-7857.2014.31.002

    高等学校博士学科点专项科研基金项目(20123250110001);国家重点基础研究发展计划(973计划)项目(2011CB100100);国家自然科学基金项目(31371632,31200943,31171187);江苏省自然科学基金项目(BK2012261);江苏省高校自然科学研究重大项目(14KJA210005);江苏省"青蓝工程"科技创新团队项目

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doi: 10.3981/j.issn.1000-7857.2014.31.002
  • 接收时间:2014-09-16
  • 首发时间:2014-11-15
  • 出版时间:2014-11-08
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  • 收稿日期:2014-09-16
  • 修回日期:2014-10-08
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徐辰武,教授,研究方向为数量遗传学与生物信息学,电子信箱:qtls@yzu.edu.cn
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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