Article(id=1242131885430747900, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242131883128074998, articleNumber=null, orderNo=3, doi=10.3981/j.issn.1000-7857.2014.12.001, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1390752000000, receivedDateStr=2014-01-27, revisedDate=1395072000000, revisedDateStr=2014-03-18, acceptedDate=null, acceptedDateStr=null, onlineDate=1399947317170, onlineDateStr=2014-05-13, pubDate=1398614400000, pubDateStr=2014-04-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1399947317170, onlineIssueDateStr=2014-05-13, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774076919172, creator=sys-migrate, updateTime=1774076919172, updator=sys-migrate, issue=Issue{id=1242131883128074998, tenantId=1146029695717560320, journalId=1146031591421210625, year='2014', volume='32', issue='12', pageStart='1', pageEnd='96', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774076918628, creator=sys-migrate, updateTime=1774076918628, updator=sys-migrate, preIssue=null, nextIssue=null, ext=null, issueFiles=null}, startPage=15, endPage=18, ext={EN=ArticleExt(id=1242131891436991251, articleId=1242131885430747900, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Different Fluorescent Protein Gene to Label Streptomyces lydicus A01, columnId=1242116903087440448, journalTitle=Science & Technology Review, columnName=Spescial Issues, runingTitle=null, highlight=null, articleAbstract=Streptomyces lydicus A01 is isolated from the soil of the suburban vegetable field in Beijing (China), and it shows a strong inhibitory activity against several plant pathogenic fungi. In order to detect the activity of the erythromycin promoter (ermE^*) in Streptomyces lydicus A01, and to obtain the labeled strain for further research, the egfp 720 bp and rfp 678 bp segments are inserted into the expression vector pIB139 separately, the recombinant vectors pIB139-EGFP and pIB139-RFP are successfully obtained. The pIB139-EGEP and pIB139-REP are transformed into Streptomyces lydicus A01 strain by intergeneric conjugation. Transformants are identified by the fluorescence observation with microscope. It is shown that the two recombinant vectors are successfully integrated into the strain A01, and that the ermE^* promoter could induce egfp and rfp gene expression in Streptomyces lydicus A01., correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=NJms27LH+rDW2OAfvKwa5g==, pdfFileSize=1116017, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. College of Life Science, Henan Normal University, Xinxiang 453007, China;
2. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
3. China Green Food Development Center, Beijing 100081, China, fund=null, authors=LIU Hui¹, WU Huiling², LIU Weicheng², LI Jinjin², CHEN Baohua³, WANG Linsong¹, authorsList=LIU Hui, WU Huiling, LIU Weicheng, LI Jinjin, CHEN Baohua, WANG Linsong), CN=ArticleExt(id=1242131890308723468, articleId=1242131885430747900, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=不同荧光蛋白基因标记利迪链霉菌, columnId=1242116902361830275, journalTitle=科技导报, columnName=专题论文, runingTitle=null, highlight=null, articleAbstract=利迪链霉菌（Streptomyces lydicus）A01 是一株分离自北京郊区对植物病原真菌具有广谱抑制作用的放线菌，在植物真菌病害防治中具有良好的应用前景。为了检测红霉素启动子在利迪链霉菌A01 中的活性，为后期对菌株A01 进行遗传改造提供技术支持，同时对生防菌A01 进行遗传标记以研究和阐明其在生态环境中的生物学行为规律，本实验采用两亲本接合的方法，将增强绿色荧光蛋白（EGFP）和红色荧光蛋白（RFP）基因片段克隆到携带红霉素启动子（ermE^*）的链霉菌表达载体pIB139 中，成功构建了以egfp 和rfp 为报告基因的重组载体pIB139-EGFP 和pIB139-RFP，并转化利迪链霉菌A01，突变株在荧光显微镜下观察到较强的绿色荧光和红色荧光，同时PCR 鉴定结果正确。这表明重组载体pIB139-EGFP 和pIB139-RFP 成功转入菌株A01，并且ermE^*启动子成功启动egfp 和rfp 基因表达。, correspAuthors=null, authorNote=刘慧，研究方向为生物化学与分子生物学，电子信箱：luckystar513@163.com, correspAuthorsNote=王林嵩，教授，研究方向为酶的结构与功能，电子信箱：wls@htu.cn, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=9CbJ7tkUfQ2VOm8wLytRRA==, pdfFileSize=1116017, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. 河南师范大学生命科学学院, 新乡 453007;
2. 北京市农林科学院植物保护环境保护研究所, 北京 100097;
3. 中国绿色食品发展中心, 北京 100081, fund=北京市自然科学基金项目（6101001）；北京市农林科学院科技创新专项（KJCX201101001）；公益性行业（农业）科研专项（200903049-07）, authors=刘慧¹, 吴慧玲², 刘伟成², 李锦锦², 陈宝桦³, 王林嵩¹, authorsList=刘慧, 吴慧玲, 刘伟成, 李锦锦, 陈宝桦, 王林嵩)}, authors=null, keywords=[Keyword(id=1242131888593253126, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242131885430747900, language=CN, orderNo=1, keyword=利迪链霉菌), Keyword(id=1242131889960596231, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242131885430747900, language=CN, orderNo=1, keyword=绿色荧光蛋白), Keyword(id=1242131890057065225, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242131885430747900, language=CN, orderNo=1, keyword=红色荧光蛋白), Keyword(id=1242131890161922827, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242131885430747900, language=CN, orderNo=1, keyword=红霉素启动子), Keyword(id=1242131891030143759, tenantId=1146029695717560320, journalId=1146031591421210625, 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