Article(id=1242131574536348342, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242131541137109894, articleNumber=null, orderNo=16, doi=10.3981/j.issn.1000-7857.2014.h1.015, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1376064000000, receivedDateStr=2013-08-10, revisedDate=1385740800000, revisedDateStr=2013-11-30, acceptedDate=null, acceptedDateStr=null, onlineDate=1397006562860, onlineDateStr=2014-04-09, pubDate=1391788800000, pubDateStr=2014-02-08, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1397006562860, onlineIssueDateStr=2014-04-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774076845054, creator=sys-migrate, updateTime=1774076845054, updator=sys-migrate, issue=Issue{id=1242131541137109894, tenantId=1146029695717560320, journalId=1146031591421210625, year='2014', volume='32', issue='4-5', pageStart='1', pageEnd='128', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774076837090, creator=sys-migrate, updateTime=1774076837090, updator=sys-migrate, preIssue=null, nextIssue=null, ext=null, issueFiles=null}, startPage=91, endPage=94, ext={EN=ArticleExt(id=1242131578365747920, articleId=1242131574536348342, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Establishment and Application of High-throughput Screening Model for SHP Inhibitors, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=The aims of this study is to set up a high throughput screening model for SHP2 inhibitors and screen the potential inhibitors. By the E. coil expression system GST-SHP2 fusion protein is cloned and over expressed. High-purity SHP2 protein is purified though GSTSepharose column. After establishing the high through screening system with the colorimetric assay of SHP2, a library of 48000 pure compounds is screened using the 384 micro- plate. Among them 75 compounds have inhibitory effects over 50%. Ultimately, three inhibitors are identified as SHP2 inhibitors with high activity. The high throughput screening is a highly sensitive, inexpensive, and operationally simple assay method in identifying SHP2 inhibitors., correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=F1OsvN0ch+M6gAXxAT4UIA==, pdfFileSize=631125, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. Department of General Thoracic Surgery, Lanzhou General Hospital of Lanzhou Military Command, Lanzhou 730050, China;
2. Key Lab of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science of Lanzhou University, Lanzhou 730020, China;
3. National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Science, Shanghai 201203, China, fund=null, authors=GAO Wei¹, SHAO Wei², SU Gang², LI Jia³, XIE Xiaodong², authorsList=GAO Wei, SHAO Wei, SU Gang, LI Jia, XIE Xiaodong), CN=ArticleExt(id=1242131577392673732, articleId=1242131574536348342, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=蛋白酪氨酸磷酸酶SHP2抑制剂高通量筛选模型的建立和应用, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为了建立体外蛋白酪氨酸磷酸酶SHP2抑制剂的高通量筛选模型，筛选潜在的SHP2抑制剂，通过应用大肠杆菌系统克隆表达GST-SHP2 融合蛋白，经过GST-Sepharose 亲和层析分离得到纯化的GST-SHP2 蛋白，建立384 孔板的高通量筛选模型，对48000个有明确结构的小分子化合物进行体外筛选，筛选出75个活性化合物对SHP2的抑制作用大于50%，确定3个化合物具有较高的抑制活性。该筛选模型灵敏、稳定，对SHP2抑制剂药物研发打下了基础。, correspAuthors=null, authorNote=高炜，主任医师，研究方向为肿瘤临床及肿瘤遗传学，电子信箱：329921879@qq.com, correspAuthorsNote=谢小冬，xdxie@lzu.edu.cn, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=zX/2uGKj+62HroCs8Rtkpg==, pdfFileSize=631125, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. 兰州军区总医院普胸外科, 兰州 730050;
2. 兰州大学基础医学院, 甘肃省新药临床前研究重点实验室, 兰州 730020;
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