Article(id=1242128992032063526, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242128972264309708, articleNumber=null, orderNo=null, doi=10.3981/j.issn.1000-7857.2012.17.012, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1321200000000, receivedDateStr=2011-11-14, revisedDate=1336579200000, revisedDateStr=2012-05-10, acceptedDate=null, acceptedDateStr=null, onlineDate=1339948800000, onlineDateStr=2012-06-18, pubDate=1339948800000, pubDateStr=2012-06-18, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1339948800000, onlineIssueDateStr=2012-06-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774076229339, creator=sys-migrate, updateTime=1774076229339, updator=sys-migrate, issue=Issue{id=1242128972264309708, tenantId=1146029695717560320, journalId=1146031591421210625, year='2012', volume='30', issue='17', pageStart='3', pageEnd='95', issueExtLink='null', onlineDate='null', pubDate='1339948800000', pubDateStr='2012-06-18', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774076224626, creator='sys-migrate', updateTime=1774076224626, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=73, endPage=79, ext={EN=ArticleExt(id=1242128997371412551, articleId=1242128992032063526, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Analytical Method for Etheno DNA Adducts, columnId=1242116810380743325, journalTitle=Science & Technology Review, columnName=Reviews, runingTitle=null, highlight=null, articleAbstract=In order to deeply study etheno-DNA adducts, the variety of DNA oxidative damages, repair mechanisms, and their products are summarized. The generative mechanism in vivo and analytical method of etheno-DNA adducts, including 1, N⁶-ethenoadenine (εAde), 1, N²- ethenoguanine (εGua), and 3, N⁴-ethenocytosine (εCyt) are reviewed. The current analytical methods of etheno-DNA adducts have been discussed, which include Immunoaffinity Chromatography/³²P-postlabelling technique (IC-³²P), Gas Chromatogeraph-Mass Spectrometer (GC-MS). and Liquid Chromatography-tandem Mass Spectrometer (LC-MS/MS). IC-³²P has the excellent performance on the sensibility, but when determining etheno-DNA adducts, complex operations and many steps have been involved. As analytical devices, the characteristics of mass spectrometer make the device obtain ideal sensibility and specificity. GC-MS method has the lower limit of determination than that for LC-MS/MS method. As sample derivatization is needed, the sample usually cost more for the method of GC-MS during the pre-handling; LC-MS/MS method offers many practical advantages on the pre-handing, stability, selectivity, and sensibility. These advantages could enhance the efficiency of samples analysis. LC-MS/MS is the ideal analytical method for the research on etheno-DNA adducts. The mechanism of DNA oxidative damage is inconclusive. As the biomarkers of DNA oxidative damages, etheno-DNA adducts possess significant meanings on the risk evaluation of lipid peroxidation., authors=TIAN Yongfeng^1,2, HOU Hongwei¹, LIU Yong², HU Qingyuan¹, WANG An², authorsList=TIAN Yongfeng;HOU Hongwei;LIU Yong;HU Qingyuan;WANG An, authorCompany=1. China National Tobacco Quality Supervision and Test Center, Zhengzhou 450001, China ;2. Institute of Optics and Fine Mechanics, Chinese Academy of Science Anhui, Hefei 230031, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=IKZiV7id2QkT46xq1uHGJQ==, pdfFileSize=1651401, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242128996511580222, articleId=1242128992032063526, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=乙烯基DNA加合物检测技术研究, columnId=1242116809164390686, journalTitle=科技导报, columnName=综述文章, runingTitle=null, highlight=null, articleAbstract=为了深入的研究乙烯基DNA加合物,本文综述了DNA氧化损伤的种类、修复机制和产物以及3种乙烯基DNA加合物：乙烯基腺嘌呤(1,N⁶-ethenoadenine,εAde)、乙烯基鸟嘌呤(1,N²-ethenoguanine,εGua)和乙烯基胞嘧啶(3,N⁴-ethenocytosine,εCyt)的体内生成机制。讨论了目前检测乙烯基DNA加合物的方法,如³²P-标记法,气相色谱-质谱法,液相色谱-串联质谱法等。³²P-标记法在检测灵敏度方面表现较优；气相色谱-质谱法分析样品需要衍生化,因此易导致样品在前处理过程中损失；液相色谱-串联质谱法具有前处理方法简单、稳定性好、选择性强、灵敏度高等优点。乙烯基DNA加合物作为生物标志物对评价生物体脂质过氧化具有重要意义。液相色谱-串联质谱法是目前在检测乙烯基DNA加合物方面较为理想的方法。, authors=田永峰^1,2, 侯宏卫¹, 刘勇², 胡清源¹, 王安², authorsList=田永峰;侯宏卫;刘勇;胡清源;王安, authorCompany=1. 国家烟草质量监督检验中心,郑州 450001;2. 中国科学院安徽光学精密机械研究所,合肥 230031, correspAuthors=null, authorNote=null, correspAuthorsNote=胡清源(中国科协所属全国学会个人会员登记号E531720008M),研究员,研究方向为烟草化学和生物标志物,电子信箱：huqy@ztri.com.cn, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=44hF+mImCqkAZVP9ahsb1g==, pdfFileSize=1651401, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, 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