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It is a kind of brown sticky pulp thing after the concentration of extract liquor by the ethanol of defatted soybean meal. Soybean molasses contains a variety of functional components. It mainly includes soybean oligosaccharides, soybean isoflavone, and other physiologically active components, which have many beneficial effects on the human health. By using monosaccharides and polysaccharides with the method of fermentation, the concentration of functional polysaccharides is improved and the purpose of purifying glycogen is achieved. One strain of yeast, that is, Saccharomyces cerevisiae C that has the function of high consumption to sucrose, and high retention rate to stachyose and raffinose is screened from four stains of yeast and one strain of lactobacillus. The optimal condition for the fermentation of soybean molasses is initial molasses sugar concentration of 9%, initial pH value of 6.5, inoculation amount of 3% and fermentation at 30℃ for 24h. The retention rates in hydrolysates of soybean molasses are 0% for sucrose, 60.90% for functional oligosaccharide, and 24.46% for glucose and fructose. And repeat stability reaches at 95% under the optimal fermentation condition., authors=ZHAO Hua1, DUAN Shenglin2, HE Congfen1, WANG Ling1, SONG Jie1, WANG Xue2, CHEN Zhirong3, authorsList=ZHAO Hua;DUAN Shenglin;HE Congfen;WANG Ling;SONG Jie;WANG Xue;CHEN Zhirong, authorCompany=1. Beijing Key Laboratory of Plant Resources Research and Development; Beijing Technology and Business University, Beijing 100048, China;2. China National Research Institute of Food & Fermentation Industries, Beijing 100027, China;3. 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科技导报 |研究论文 2011 , 29 (19) : 24 -28
发酵法提纯大豆糖蜜中低聚糖的菌株筛选
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赵华1, 段盛林2, 何聪芬1, 王领1, 宋杰1, 王雪2, 陈志蓉3
作者信息
    1. 北京工商大学;北京市植物资源研究开发重点实验室,北京 100048;2. 中国食品发酵工业研究院,北京 100027;3. 中国食品药品检定研究院,北京 100050
通讯作者:
陈志蓉,助理研究员,研究方向为化妆品卫生化学,电子信箱:chenzr@sfda.gov.cn
Purification of Oligosaccharides Through Fermentation Technology with Screening of Strains
  • ZHAO Hua1, DUAN Shenglin2, HE Congfen1, WANG Ling1, SONG Jie1, WANG Xue2, CHEN Zhirong3
  • Affiliations
      1. Beijing Key Laboratory of Plant Resources Research and Development; Beijing Technology and Business University, Beijing 100048, China;2. China National Research Institute of Food & Fermentation Industries, Beijing 100027, China;3. National Institutes for Food and Drug Control, Beijing 100050, China
    出版时间: 2011-07-08 doi: 10.3981/j.issn.100-7857.2011.19.002
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    大豆糖蜜是大豆浓缩蛋白生产的副产物,是脱脂豆粕乙醇萃取液经浓缩后形成的一种棕色黏稠的浆状物,含有多种功能性成分,主要有大豆低聚糖、大豆异黄酮等生理活性物质,对人体健康有着许多有益的作用。本文运用发酵法消化大豆糖蜜中的单糖和多糖,提高功能性多糖浓度,达到糖原纯化的目的,对1种乳酸菌、4种酵母菌进行筛选试验,筛选出消耗蔗糖最多,同时水苏糖、棉子糖等低聚糖保留率最高的1种酵母菌,即酿酒酵母C。其最佳发酵条件为,糖蜜初始糖浓度9%,初始pH值6.5,接种酿酒酵母C的接种量3%,在30℃的条件下发酵24h。大豆糖蜜发酵液中各糖分的保留率为:蔗糖0%,功能性低聚糖60.90%,果糖和葡萄糖含量24.46%,发酵的重复稳定性高达95%以上。
    功能性低聚糖  /  大豆糖蜜  /  发酵
    Soybean molasses is the by-product of the production of concentrated proteins in soybeans. It is a kind of brown sticky pulp thing after the concentration of extract liquor by the ethanol of defatted soybean meal. Soybean molasses contains a variety of functional components. It mainly includes soybean oligosaccharides, soybean isoflavone, and other physiologically active components, which have many beneficial effects on the human health. By using monosaccharides and polysaccharides with the method of fermentation, the concentration of functional polysaccharides is improved and the purpose of purifying glycogen is achieved. One strain of yeast, that is, Saccharomyces cerevisiae C that has the function of high consumption to sucrose, and high retention rate to stachyose and raffinose is screened from four stains of yeast and one strain of lactobacillus. The optimal condition for the fermentation of soybean molasses is initial molasses sugar concentration of 9%, initial pH value of 6.5, inoculation amount of 3% and fermentation at 30℃ for 24h. The retention rates in hydrolysates of soybean molasses are 0% for sucrose, 60.90% for functional oligosaccharide, and 24.46% for glucose and fructose. And repeat stability reaches at 95% under the optimal fermentation condition.
    functional oligosaccharide  /  soybean molasses  /  fermentation
    赵华;段盛林;何聪芬;王领;宋杰;王雪;陈志蓉. 发酵法提纯大豆糖蜜中低聚糖的菌株筛选. 科技导报, 2011 , 29 (19) : 24 -28 . DOI: 10.3981/j.issn.100-7857.2011.19.002
    ZHAO Hua;DUAN Shenglin;HE Congfen;WANG Ling;SONG Jie;WANG Xue;CHEN Zhirong. Purification of Oligosaccharides Through Fermentation Technology with Screening of Strains[J]. Science & Technology Review, 2011 , 29 (19) : 24 -28 . DOI: 10.3981/j.issn.100-7857.2011.19.002

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    doi: 10.3981/j.issn.100-7857.2011.19.002
    • 接收时间:2010-10-13
    • 首发时间:2011-07-08
    • 出版时间:2011-07-08
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    • 收稿日期:2010-10-13
    • 修回日期:2011-05-11
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    陈志蓉,助理研究员,研究方向为化妆品卫生化学,电子信箱:chenzr@sfda.gov.cn
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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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