Article(id=1242120686924271743, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242120678661489640, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1271174400000, receivedDateStr=2010-04-14, revisedDate=1282579200000, revisedDateStr=2010-08-24, acceptedDate=null, acceptedDateStr=null, onlineDate=1286899200000, onlineDateStr=2010-10-13, pubDate=1286899200000, pubDateStr=2010-10-13, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1286899200000, onlineIssueDateStr=2010-10-13, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774074249246, creator=sys-migrate, updateTime=1774074249246, updator=sys-migrate, issue=Issue{id=1242120678661489640, tenantId=1146029695717560320, journalId=1146031591421210625, year='2010', volume='28', issue='19', pageStart='3', pageEnd='140', issueExtLink='null', onlineDate='null', pubDate='1286899200000', pubDateStr='2010-10-13', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774074247277, creator='sys-migrate', updateTime=1774074247277, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=73, endPage=76, ext={EN=ArticleExt(id=1242120694645981214, articleId=1242120686924271743, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Preparation of Carboxyl Magnetic Fluid and Surface Modification by Bovine Serum Albumin, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=Magnetic nanoparticles are now used in magnetic-mediated hyperthermia for tumor treatment. Magnetic Fe3O4 nanoparticles with a mean diameter of 10~15nm, in the range of superparamagnetism, are normally prepared by the traditional method of co-precipitation from ferrous and ferric electrolyte solutions. This work presents a novel and efficient way to prepare a stable aqueous nanomagnetite dispersion. A stable ferrofluid containing Fe3O4 nanoparticles coated by carboxyl groups was synthesized via improved co-precipitation in the presence of polyacrylic acid. Furthermore, Bovine Serum Albumin (BSA) used as the model targeting molecule was conjugated with magnetic nanoparticles via activation by N-ethyl-N'-(3-dimethylamino-propyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide(NHS). The results indicate that the average diameter of PAA oligomers coated Fe3O4 particles was about 10nm, and had a good dispersion in aqueous phase. In addition, both quantitative analysis and fluorescent microscopy observation confirmed that BSA can be stably conjugated with carboxyl-Fe3O4 particles via EDC and NHS activation., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=wPGUeZiyHytXueokXLnTYg==, pdfFileSize=791566, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242120693714849928, articleId=1242120686924271743, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=羧基磁流体制备和牛血清白蛋白表面修饰, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=磁性纳米粒已被用作肿瘤磁感应热疗的介质，目前常用纳米Fe3O4，其经典制备方法是化学共沉淀法，得到具有超顺磁性的纳米Fe3O4粒径约为10～15nm。本文采用改进的化学共沉淀法制备聚丙烯酸（PAA）修饰的羧基纳米Fe3O4，通过EDC、NHS活化法偶联模型蛋白牛血清白蛋白（BSA），用BCA定量分析磁粒对BSA的偶联效率，并进一步偶联荧光标记的抗体，观察偶联效果。结果表明，改进的化学共沉淀法制备的羧基纳米粒粒径约10nm，水相分散性良好；定量分析和光学观察结果均显示，采用EDC、NHS活化法能使蛋白质与羧基化磁性颗粒稳定结合。本研究为靶向磁性纳米粒的制备提供一种新方法。, authors=^{闫 妍1,2，赵凌云1，王晓文1，张晓冬1，高福平1，唐劲天1}, authorsList=闫 妍;赵凌云;王晓文;张晓冬;高福平;唐劲天, authorCompany=1. 清华大学工程物理系；粒子技术与辐射成像教育部重点实验室，北京1000842. 北京中医药大学中药学院，北京100102, correspAuthors=null, authorNote=null, correspAuthorsNote=唐劲天, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=dCAflw02UdSyiIndinaWyA==, pdfFileSize=791566, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=null, keywords=[Keyword(id=1242120692875984899, 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