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科技导报
|研究论文
2010
, 28
(03) :
51
-54
应用16SrDNA序列分析对砾石接触氧化反应器中细菌多样性
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付丽丽
作者信息
辽宁石油化工大学石油天然气工程学院，辽宁抚顺 113001
通讯作者:
付丽丽
Analysis of Bacterial Diversity of Gravel Contact Oxidation Reactor by 16SrDNA Sequencing
Affiliations
出版时间: 2010-02-13
文章导航
运用分子生物学手段，对砾石接触反应器内的典型区域，包括石球表面的生物膜、石球内部污泥及反应器底部的沉淀污泥，通过DNA提取、PCR扩增、纯化与回收、TA克隆、序列测定，基因比对等方法，对反应器内的细菌多样性进行分析。结果表明，系统中主要存在好氧呼吸菌群及厌氧发酵菌群两大类，优势菌群为以呼吸代谢为主的假单胞菌属、以发酵为主要代谢方式的拟杆菌/噬纤维菌菌属和纤毛菌属。同时好氧菌群中还发育有α蛋白菌、β蛋白菌、γ蛋白菌、硝化螺菌属、土壤杆菌属、衣原体属、葡萄糖杆菌属及褐色高温单孢菌属细菌；厌氧菌群中则发育属于螺旋体属、δ蛋白菌、反硝化菌、乳杆菌属；此外，使生物量的增长减少的各种慢速生长菌群也是本系统内的一大特色菌群。生物多样性的存在，是污泥减量化研究的关键。
Microbes are important members in a biological treatment system, especially, in the gravel contact oxidation reactor filled with crushed stone globular aggregates as carriers, which is discussed in this paper. By means of molecular biology techniques, for the gravel contact reactor, in its typical area, including the surface biological membrane of gravel, internal sludge of gravel and bottom sludge of the reactor, after DNA extraction, PCR and cloning, 16SrDNA sequence determination, the bacterial diversity is analyzed. The results indicate that the reactor bacterial communities are composed of aerobic bacteria group and anaerobic bacteria group. The dominant members are respiratory metabolized microbes Pseudomonas and fermentative metabolized microbes Bacteroidetes bacterium/Cytophaga. Non-dominant members in the aerobic group include α-, β-, and γ-Proteobacterium, Nitrosospira, Chlamydia, Gluconobacter and Agrobacterium. Anaerobic members are Spirochaeta Sp., delta preobacterium, Denitrifying bacterium. In addition, to reduce the growth of biomass, the slow-growth bacterial group is also a feature group in this reactor. The existence of biodiversity is the key of the sludge reduction.
bacterial diversity
/
16SrDNA
/
gravel contact Oxidation
付丽丽.
应用16SrDNA序列分析对砾石接触氧化反应器中细菌多样性.
科技导报,
2010
, 28
(03)
: 51
-54
.
.
Analysis of Bacterial Diversity of Gravel Contact Oxidation Reactor by 16SrDNA Sequencing[J].
Science & Technology Review ,
2010
, 28
(03)
: 51
-54
.
2010年第28卷第03期
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文章信息
接收时间：2010-01-06
首发时间：2010-02-13
出版时间：2010-02-13
收稿日期：2010-01-06
修回日期：2010-01-20
https://castjournals.cast.org.cn/joweb/kjdb/CN/1242119529866789041
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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