Article(id=1242118871503671397, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242118860854330001, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1251043200000, receivedDateStr=2009-08-24, revisedDate=-2209017600000, revisedDateStr=1900-01-01, acceptedDate=null, acceptedDateStr=null, onlineDate=1123603200000, onlineDateStr=2005-08-10, pubDate=1123603200000, pubDateStr=2005-08-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1123603200000, onlineIssueDateStr=2005-08-10, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774073816414, creator=sys-migrate, updateTime=1774073816414, updator=sys-migrate, issue=Issue{id=1242118860854330001, tenantId=1146029695717560320, journalId=1146031591421210625, year='2005', volume='23', issue='0508', pageStart='1', pageEnd='81', issueExtLink='null', onlineDate='null', pubDate='1123603200000', pubDateStr='2005-08-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774073813878, creator='sys-migrate', updateTime=1774073813878, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=31, endPage=34, ext={EN=ArticleExt(id=1242118878210360009, articleId=1242118871503671397, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=A Preliminary Study on Transgenic Mouse Model of Human Lactoperoxidase, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=Human Lactoperoxidase gene (hLPO) has been cloned into pBC1 to construct pBC1-hLPO specialized expression vector in mammary glands. Human LPO gene was assembled by two gene fragments, which are 3 kilo base pairs in length and 27 kilo base pairs long respectively. The shorter one was amplified by long-distance PCR and the other was cut down by restriction AgeI, both based on the donor of PAC RP5-1171I10 plasmid that contains hLPO gene. Here, We established the transgenic mouse model by microinjection into fertilized eggs, and then obtained 5 transgenic mice (4male, 1female) out of 28 descendants, identified by PCR and Southern blot. The integration rate is 17.86%, and lactoperoxidase expression levels were determined by SDS-PAGE gel electrophoresis and Western blot. The hLPO recombinant protein band is not obvious as the antibodies are not very specific., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=5kFZyLg24OTMOOYKFRNMnA==, pdfFileSize=473785, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242118875693781100, articleId=1242118871503671397, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=转基因小鼠乳腺表达人乳过氧化物酶的初步研究, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=从人基因组PAC文库中筛选出人乳过氧化物酶(hLPO)基因,利用长片段PCR的方法获得hLPO基因5'端约3kb片段,通过酶切方法获得hLPO基因3'端约27kb片段,将这两部分拼接并克隆到乳腺特异性表达载体pBC1上,构建以山羊β-casein启动区指导的hLPO的转基因表达载体pBC1-hLPO。利用显微注射的方法获得28只F0代小鼠,经PCR检测和Southern杂交分析证实,有5只小鼠(4♂,1♀)为整合hLPO基因的转基因阳性小鼠,整合率为17.86%,整合拷贝数在1至5之间。利用SDS-PAGE凝胶电泳和Westernblot印迹分析F0、F1代共3只雌性转基因小鼠乳样,结果表明hLPO重组蛋白的特异条带不明显。, authors=余珊珊¹,王毅彰²,于舒洋¹,李宁¹, authorsList=余珊珊;王毅彰;于舒洋;李宁, authorCompany=1. 中国农业大学农业生物技术国家重点实验室 北京2. 安阳师范学院地理系 河南安阳, correspAuthors=null, authorNote=null, correspAuthorsNote=余珊珊, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=qwB33ALYnAvo2UqpQdJvYw==, pdfFileSize=473785, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=null, keywords=[Keyword(id=1242118875375010488, 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