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The result shows that the Senegal bichir Shh gene is of 1 263 bp in length，with 421 amino acids. From a comparison by homology, it can be seen that lungfish and Senegal bichir share the highest homology of 82.5%. A secondary structure analysis shows Shh albumen consists of 30.71% of α helix, 21.67% of extended line and 47.62% of random coil, without β slice. To construct the three dimensional structure of Shh protein by homology using http://www.expasy.org/swissmod/SWISS-MODEL.html one establishes a good foundation for further studies on Shh gene functions in the embryonic development and the limb bud schema., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=IB0OS8SeJ58RPZ5m5AmkOQ==, pdfFileSize=1126374, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242118435941978639, articleId=1242118433547031042, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=多鳍鱼Shh基因克隆与同源性分析, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=根据已报道的软骨鱼、硬骨鱼、两栖类、鸟类、哺乳类和人的Sonic hedgehog (Shh)基因序列，在Shh基因保守区设计一对简并性引物，利用PCR技术从多鳍鱼cDNA中扩增Shh基因。结果显示，多鳍鱼Shh基因全长1 263bp，编码421个氨基酸。通过同源性比较发现，多鳍鱼与肺鱼的同源性最高，为82.5%，二级结构分析表明，Shh蛋白含有30.71%的α螺旋，21.67%的扩展线条和47.62%的无规卷曲，不含β片层；并以蛋白质结构数据库（PDB）中已解析的Shh蛋白的三维结晶结构(3D)为模板，利用http://www.expasy.org/swissmod/SWISS-MODEL.html同源模建Shh蛋白的三维结构，为进一步研究Shh基因在胚胎体节发育和肢芽图式形成中的功能奠定了基础。, authors=张昌盛1，2,刘群1,刘湘涛2,翟红2,王俊杰2,孙永华2, authorsList=张昌盛;刘群;刘湘涛;翟红;王俊杰;孙永华, authorCompany=1中国农业大学动物医学院 北京 2北京自然博物馆 北京, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=YXcUwlym/uHV0x3eP8IMjg==, pdfFileSize=1126374, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, 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