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吴茱萸超微饮片ITS序列指纹图谱的研究
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吴茱萸超微饮片ITS序列指纹图谱的研究
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李顺祥1,2,蔡光先1,2,张平3,韦里俊1,2,3,黄丹2
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    1. 湖南省中医药研究院 长沙2. 湖南中医药大学 长沙 3湖南师范大学 长沙
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李顺祥
Study on ITS Sequence Fingerprint of Ultramicro Fructus Evodiae
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    出版时间: 2007-03-10
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    为探求吴茱萸超微饮片的品种鉴别方法,以来自不同产地的5个吴茱萸样品、7个疏毛吴茱萸和3个石虎样品制成的超微饮片为实验材料,用分子克隆方法获得并测定其ITS序列。标记它们的ITS1,5.8 s,ITS2的全长序列,构建了吴茱萸的ITS序列指纹图谱,得出吴茱萸超微饮片3个不同植物来源种内相似度在98%以上;吴茱萸和其变种疏毛吴茱萸及石虎之间的ITS序列有显著的差异,可达27%,而疏毛吴茱萸和石虎的序列差异较小,但是在两组序列的对比中,发现有8个特异位点,显示了这两个亲缘关系比较相近的药材的区别。以ITS序列测定与分析可作为吴茱萸超微饮片的品种鉴定和质量控制的方法。
    中药学  /  超微饮片  /  吴茱萸  /  ITS序列  /  品种鉴定
    To study the identification and evaluation methods of Ultramicro Fructus Evodiae, samples of Ultramicro Fructus Evodiae were taken, respectively, from fifteen kinds of Evodia Rutaecarpa (Juss.) Benth., and its varietas, their total DNAs were extracted and cloned, and the ITS sequencee were determined by molecular biological techniques. The ITS1, 5.8S and ITS2 sequences of ultramicro Fructus evodiae from three plant species were obtained. The inner similitude of same species was above 98%, but the diversity among these sequences was about 27%. Analysis of ITS sequence fingerprint can provide an effective criterion for the identification of Ultramicro prepared herbs.
    李顺祥;蔡光先;张平;韦里俊;;黄丹. 吴茱萸超微饮片ITS序列指纹图谱的研究. 科技导报, 2007 , 25 (0705) : 42 -46 .
    . Study on ITS Sequence Fingerprint of Ultramicro Fructus Evodiae[J]. Science & Technology Review, 2007 , 25 (0705) : 42 -46 .

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    • 收稿日期:2008-12-16
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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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