Article(id=1242117959590674626, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242117952087069208, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1249142400000, receivedDateStr=2009-08-02, revisedDate=-2209017600000, revisedDateStr=1900-01-01, acceptedDate=null, acceptedDateStr=null, onlineDate=1152460800000, onlineDateStr=2006-07-10, pubDate=1152460800000, pubDateStr=2006-07-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1152460800000, onlineIssueDateStr=2006-07-10, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774073598997, creator=sys-migrate, updateTime=1774073598997, updator=sys-migrate, issue=Issue{id=1242117952087069208, tenantId=1146029695717560320, journalId=1146031591421210625, year='2006', volume='24', issue='0607', pageStart='1', pageEnd='94', issueExtLink='null', onlineDate='null', pubDate='1152460800000', pubDateStr='2006-07-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774073597209, creator='sys-migrate', updateTime=1774073597209, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=54, endPage=58, ext={EN=ArticleExt(id=1242117963625595104, articleId=1242117959590674626, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Experimental Research of the Treatment of Chronic Damaged Liver by Transplantation of Mouse Embryonic Germ Cells, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=The EGCs were proliferated and labeled with 5-bromo-2-deoxyuridine (BrdU), then transplanted into the acute damaged liver by CCL4 through tail vein. 2 and 4 weeks later, the liver was extracted and 10μm-cryostat continuous sections were obtained. The existing and differentiation of the transplanted cells were identified by immunohistochemistry, immunoflorescence double staining and PAS histochemistry for BrdU and hepatic-specific albumin (ALB), and the glycogen. The BrdU positive cells were found in the chronic damaged liver, some cells were BrdU and ALB double positive and PAS positive staining , the structures of damaged liver were also significantly improved. We demonstrated that the transplanted EGCs could be incorporated into the chronic damaged liver and differentiated into hepatocytes in this microenvironment, and its transplantation also could compensate for chronic liver failure. These results suggest that EGCs can be used for potential cellular hepatoplasty to treat damaged l...更多iver., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=3U+vtgDlN+Z/xHYMxX6JFA==, pdfFileSize=707803, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242117961385841222, articleId=1242117959590674626, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=小鼠胚芽干细胞同种移植治疗慢性肝脏损伤的实验研究, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=将分离和体外扩增的小鼠胚芽干细胞,用BrdU标记后,经尾静脉同种移植于CCL4制备慢性肝损伤小鼠体内。分别于移植后2w、4w取出肝脏,进行连续冰冻切片,用免疫组织化学和免疫荧光双重染色检测移植细胞在受体小鼠肝内的存活、分布和肝细胞特异性白蛋白的表达;用PAS染色检测移植细胞的糖原代谢;HE染色观察移植动物肝组织的结构变化。探索胚芽干细胞在慢性损伤肝脏中的存活、分化和治疗作用。实验结果表明:胚芽干细胞经静脉移植后可以进入损伤肝脏中,并分化为肝样细胞和肝小叶内的其他细胞,并明显改善损伤肝脏的组织结构,具有较好的治疗作用。, authors=刘晓萍¹,高英茂²,邴鲁军²,郭菲菲¹,徐珞¹, authorsList=刘晓萍;高英茂;邴鲁军;郭菲菲;徐珞, authorCompany=1. 青岛大学医学院组织胚胎学教研室 山东青岛2. 山东大学医学院组织胚胎学教研室 济南, correspAuthors=null, authorNote=null, correspAuthorsNote=高英茂, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=hGurxsEF9hfSBsUW46F47g==, pdfFileSize=707803, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=[Author(id=1299571019803554318, tenantId=1146029695717560320, journalId=null, articleId=1242117959590674626, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={}, companyList=null)], keywords=[Keyword(id=1242117960978993725, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=CN, orderNo=1, keyword=胚芽干细胞), Keyword(id=1242117961041908287, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=CN, orderNo=1, keyword=细胞移植), Keyword(id=1242117961155154498, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=CN, orderNo=1, keyword=慢性肝脏损伤), Keyword(id=1242117961243234885, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=CN, orderNo=1, keyword=小鼠), Keyword(id=1242117961843015899, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=EN, orderNo=1, keyword=embryonic germ cells), Keyword(id=1242117961910124764, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=EN, orderNo=1, keyword=cell transplantation), Keyword(id=1242117961981427933, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=EN, orderNo=1, keyword=damaged liver), Keyword(id=1242117962056925406, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242117959590674626, language=EN, orderNo=1, keyword=mouse)], refs=null, funds=null, companyList=null, figs=null, attaches=null, journal=Journal(id=1125356956822126595, delFlag=0, nameCn=科技导报, nameEn=Science & Technology Review, nameHistory1=null, nameHistory2=null, issn=1000-7857, eissn=, cn=11-1421/N, coden=null, periodic=3, language=CN, oaType=0, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=wfghvu3bhh/dKxuZ+ucVHA==, journalPrice=null, startedYear=null, abbrevIsoEn=Sci Technol Rev, journalRemark=null, publicationField=null, createdTime=null, updatedTime=1784015846012, createdBy=null, updatedBy=13041195026, firstLetterCn=K, firstLetterEn=K, subjectCode=Natural Sciences, subjectName=自然科学, subjectCodeEn=Natural Sciences, subjectNameEn=null, picCn=wfghvu3bhh/dKxuZ+ucVHA==, picEn=yjSfclmpNm7ihn9NbTZ69g==, jcr=null, cjcr=null, exts=[JournalExt(id=1283818766098219763, language=CN, name=科技导报, nameHistory1=null, nameHistory2=null, managedBy=中国科学技术协会, sponsoredBy=中国科学技术协会, publishedBy=科技导报社, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=http://www.kjdb.org/CN/home, createdTime=1784015846037, updatedTime=1784015846037, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=http://www.kjdb.org/CN/column/column7.shtml, submissionAuthorUrl=https://kjdbauthor.cast.org.cn/webm, submissionEditorUrl=https://kjdbeditor.cast.org.cn/webm/, submissionReviewUrl=https://kjdbauthor.cast.org.cn/webm, submissionCeEditorUrl=https://kjdbeditor.cast.org.cn/webm/, submissionAeEditorUrl=https://kjdbeditor.cast.org.cn/webm/, option={"copyright":""}), JournalExt(id=1283818766144357108, language=EN, name=Science & Technology Review, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=http://www.kjdb.org/EN/home, createdTime=1784015846048, updatedTime=1784015846048, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=http://www.kjdb.org/EN/column/column7.shtml, submissionAuthorUrl=https://kjdbauthor.manuscriptcloud.com/login, submissionEditorUrl=https://kjdbeditor.manuscriptcloud.com/login, submissionReviewUrl=https://kjdbauthor.manuscriptcloud.com/login, submissionCeEditorUrl=https://kjdbeditor.manuscriptcloud.com/login, submissionAeEditorUrl=https://kjdbeditor.manuscriptcloud.com/login, option={"copyright":""})], databaseList=null, tenantJournalId=1146031591421210625, websiteList=[Website(id=1146104741081231361, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1146031591421210625, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/kjdb/CN, language=CN, createTime=1751182263881, createBy=18614031015, updateTime=1751778001962, updateBy=18614031015, name=科技导报, tplId=1146099689490845704, title=科技导报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1148021146403992296, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146104741081231361, code=articleTextType, value=kx, createTime=1751639170504, updateTime=1751639170504, creator=18614031015, updator=18614031015), WebsiteProps(id=1148021146378826469, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146104741081231361, code=banner, value=null, createTime=1751639170498, updateTime=1751639170498, creator=18614031015, updator=18614031015), WebsiteProps(id=1148021146366243556, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146104741081231361, code=logo, value=https://castjournals.cast.org.cn/joweb/kjdb/CN/file/pic?fileId=9GHSf7eGlIPH0Tv/OOdstA==, createTime=1751639170495, updateTime=1751639170495, creator=18614031015, updator=18614031015), WebsiteProps(id=1148021146395603687, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146104741081231361, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/kjdb/CN/file/pic, createTime=1751639170502, updateTime=1751639170502, creator=18614031015, updator=18614031015), WebsiteProps(id=1148021146387215078, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146104741081231361, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1751639170500, updateTime=1751639170500, creator=18614031015, updator=18614031015)]), Website(id=1146105254833139715, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1146031591421210625, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/kjdb/EN, language=EN, createTime=1751182386363, createBy=18614031015, updateTime=1753500121937, updateBy=18614031015, name=科技导报, tplId=1146101810881728533, title=Science & Technology Review, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1155838567709528217, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146105254833139715, code=articleTextType, value=kx, createTime=1753502988984, updateTime=1753502988984, creator=18614031015, updator=18614031015), WebsiteProps(id=1155838567692750998, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146105254833139715, code=banner, value=null, createTime=1753502988980, updateTime=1753502988980, creator=18614031015, updator=18614031015), WebsiteProps(id=1155838567688556693, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146105254833139715, code=logo, value=https://castjournals.cast.org.cn/joweb/kjdb/EN/file/pic?fileId=9GHSf7eGlIPH0Tv/OOdstA==, createTime=1753502988979, updateTime=1753502988979, creator=18614031015, updator=18614031015), WebsiteProps(id=1155838567705333912, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146105254833139715, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/kjdb/EN/file/pic, createTime=1753502988983, updateTime=1753502988983, creator=18614031015, updator=18614031015), WebsiteProps(id=1155838567701139607, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1146105254833139715, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1753502988982, updateTime=1753502988982, creator=18614031015, updator=18614031015)])], journalTitle=科技导报, weixinUrl=null, journalUrl=null, iacademicId=null, status=1, seqNo=null, journalTitleEn=Science & Technology Review, journalPhotoCn=wfghvu3bhh/dKxuZ+ucVHA==, journalPhotoEn=yjSfclmpNm7ihn9NbTZ69g==, journalFirstLetter=K, journalRecommend=null, journalNew=null, journalCollection=1, jcrJf=null, cjcrJf=0.91, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/kjdb/CN/Y2006/V24/I0607/54, detailUrlEn=https://castjournals.cast.org.cn/joweb/kjdb/EN/Y2006/V24/I0607/54, pdfUrlCn=https://castjournals.cast.org.cn/joweb/kjdb/CN/PDF/Y2006/V24/I0607/54, pdfUrlEn=https://castjournals.cast.org.cn/joweb/kjdb/EN/PDF/Y2006/V24/I0607/54, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1152460800000, fullTextJson=null, articleText=null, reference=null)