Article(id=1242117940783419857, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242117924060725272, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1250006400000, receivedDateStr=2009-08-12, revisedDate=-2209017600000, revisedDateStr=1900-01-01, acceptedDate=null, acceptedDateStr=null, onlineDate=1155139200000, onlineDateStr=2006-08-10, pubDate=1155139200000, pubDateStr=2006-08-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1155139200000, onlineIssueDateStr=2006-08-10, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774073594514, creator=sys-migrate, updateTime=1774073594514, updator=sys-migrate, issue=Issue{id=1242117924060725272, tenantId=1146029695717560320, journalId=1146031591421210625, year='2006', volume='24', issue='0608', pageStart='1', pageEnd='94', issueExtLink='null', onlineDate='null', pubDate='1155139200000', pubDateStr='2006-08-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774073590529, creator='sys-migrate', updateTime=1774073590529, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=31, endPage=35, ext={EN=ArticleExt(id=1242117944184995978, articleId=1242117940783419857, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Differential Gene Expression of Uterine Cervix Cancer Cell Induced by Exposing to the Space Environment, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=We investigated the effect of special space environment on cell growth characteristics and malignant phenotype in the Caski human uterine cervix cancer cell line. Caski cell was carried in "Shenzhou Ⅳ" spacecraft and the returning cell was monocoloned. Strains numbered 44F10 was screened out since its increased cell proliferation and tumorigenesis. Moreover, its cell cycle was induced to progress from G1 to S phase, while strains 48A9 were opposite to 44F10 in cytological events. The difference between ground control groups and space groups was significant (P<0.05). Little is known about the underlying changes in gene expression. The purpose of this study was to investigate these changes. Oligonucleotide microarray containing 2 747 probesets was performed on 4410/ control cell and 48A9/ control cell. Total RNA from both cell types was isolated for reversed transcription cDNA probes; hybridization was performed with double pathway-specific gene expression profiling array membrane. Compared with ground control cell, 16 genes were detected to be differentially expressed in group 44F10. There are 36 genes exhibiting differential expression in group 48A9. Among these genes, a high proportion of genes were associated with apoptotic, cell cycle, cell proliferation and cell signal transduction. As expected, several growth-promoting genes were upregulated in group 44F10, while many genes associated with growth restriction were upregulated in group 48A9. The study provides that differential gene expression of uterine cervix cancer cell induced by exposing to the space environment can lead to alteration of phenotype., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=lK/TWgcSSj0LhTGsjyOQ0g==, pdfFileSize=208123, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242117943409049727, articleId=1242117940783419857, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=太空诱变宫颈癌细胞的差异表达基因初探, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=将搭载于“神舟四号”飞船飞行返地后的宫颈癌Caski细胞进行单克隆化,筛选出生长速度快于对照组、编号为44F10的细胞克隆,G1期细胞减少,S期细胞增多,成瘤能力增强;编号为48A9的细胞克隆细胞学行为与之相反,与对照组差异均有显著性(P<0.05)。为了解太空诱变肿瘤细胞生物学行为改变的机制,从分子水平入手研究经太空诱变的宫颈癌细胞和地面对照细胞的差异表达基因。应用含2747个人类肿瘤相关基因的Oligo双通道芯片研究差异表达基因。分别抽提44F10、48A9组和地面对照细胞的总RNA,逆转录cDNA并标记探针。将实验组和对照组cDNA探针混合,分别与同一张芯片杂交后,用不同的波长扫描荧光强度,从而筛选出差异基因。44F10组有16个基因呈现差异表达,48A9组有36个基因呈现差异表达。差异性表达主要涉及细胞凋亡、细胞增殖、细胞周期调控和信号转导的基因。促进细胞增殖的基因在44F10组中表达上调,而在48A9组中限制细胞增殖的基因表达上调。研究表明,太空诱变宫颈癌细胞的差异表达基因导致了细胞生物学行为的改变。, authors=杨丞,李官成, authorsList=杨丞;李官成, authorCompany=中南大学肿瘤研究所免疫室 长沙, correspAuthors=null, authorNote=null, correspAuthorsNote=杨丞, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=bapIgyoZ7Z7ova0rrjIV1g==, pdfFileSize=208123, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, 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